specificity within the ets family of transcription factors
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
Ngày tải lên :
21/02/2014, 03:20
... which transcription factor of the Ets- family is involved in the regulation The members of the Ets- family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T ... upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene We identified two transcription ... sequence was detected in EMSA These results suggest that the transcription of the mouse integrin a3 subunit gene is regulated by the Ets- family transcription factors in these cells A homology search...
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Báo cáo y học: "The AP-2 family of transcription factors." potx
Ngày tải lên :
14/08/2014, 16:20
... the development of these structures [7,31-33] In zebrafish, the two AP-2 family members, tfap2a and tfap2b [34], are coexpressed in the neural tube, the ectoderm and the pronephric ducts of the ... http://genomebiology.com/2005/6/13/246 The lethal phenotypes of the AP-2 mutants generated so far have precluded an analysis of the roles of AP-2 transcription factors in adult tissues We and others are currently exploiting the ... modulating the transactivation capability or DNA-binding affinity of the other AP-2 family members The crystal structure of the AP-2 proteins has not yet been solved Localization and function AP-2 transcription...
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Báo cáo khoa học: Structure of FocB – a member of a family of transcription factors regulating fimbrial adhesin expression in uropathogenic Escherichia coli pdf
Ngày tải lên :
15/03/2014, 23:20
... member of the PapB family of fimbrial transcriptional factors The members of the PapB family identified so far exist in both the E coli and Salmonella species and are all involved in the regulation of ... between base pair steps in the DNA is the main cause of the extreme DNA-bending ability of these proteins [48] Also, LacI [49,50] and PurR [51], members of the LacI family, bend their operator DNA Both ... In many respects, the roles of the FaeB and FanB proteins in transcriptional regulation are similar to that of PapB in the regulation of the pap operon [20,21] UPEC strains often carry several...
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Báo cáo khoa học: The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw potx
Ngày tải lên :
30/03/2014, 16:20
... with activation of FRM2 transcription, but also with growth in increasing concentrations of cadmium but not of H2O2 Because of the high degree of homology to Yap1, the role of the Yap2 cysteine ... the formation of an intermolecular bond occurs between the Orp1 Cys36 and the Cys598 of Yap1 leading to its activation The subsequent formation of the Yap1 Cys303-Cys598 disulfide bond masks the ... different forms of stress highlighting, in particular, those in which the Yap family of basic-leucine zipper (b-ZIP) transactivators play a role The Yap protein family The Yap family of b-ZIP proteins...
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Báo cáo khoa học: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction doc
Ngày tải lên :
23/03/2014, 10:20
... factors [12] One of the key determinants of the activation of genes that are under the control of hormone response units is the order and time at which the transcription factors bind to their response ... liver-enriched factors FoxA and C ⁄ EBP, they differ in their recruitment of these common transcription factors Although it is still unclear what determines these differences in the assembly of the transcription ... that these features underlie the absence of binding of transcription factors (Fig 3) and the lack of CPS expression in Rat-1 cells In vivo footprinting of FTO-2B hepatoma cells, on the other...
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Báo cáo khoa học: Conformational stability of neuroglobin helix F – possible effects on the folding pathway within the globin family potx
Ngày tải lên :
30/03/2014, 01:20
... intervals Therefore, the cleavage location is partially dependent on the incubation time of the substrate with the protease, at least within the limits of the experimental protocol Cleavage at the ... processes of the apo form of the protein (apoNgb) as a result of the formation of an intermolecular disulfide bond The preparation of apoNgb was obtained from the corresponding holoprotein by the removal ... proteases The limited proteolysis pattern also led to the establishment that no other potential cleavage sites of apoMb undergo hydrolysis, as a consequence of the stability of the helical fold of the...
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báo cáo khoa học: "A single amino acid change within the R2 domain of the VvMYB5b transcription factor modulates affinity for protein partners and target promoters selectivity" docx
Ngày tải lên :
11/08/2014, 11:21
... branches of flavonoid biosynthesis are under the transcriptional control of different MYBs proteins [15-21] Among them, two MYB transcription factors, VvMYB5a and VvMYB5b, contribute to the transcriptional ... http://www.biomedcentral.com/1471-2229/11/117 Page of 14 Figure Structure of the R2R3 domain of different MYB proteins (A) Protein sequence alignment of the R2R3 domain of grapevine MYB transcription factors regulating the flavonoid ... transcriptional regulation of the flavonoid pathway have been published (reviewed in [8,10]) Most of them emphasized the pivotal role of MYB transcription factors in the control of this metabolic pathway...
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Analysis of transcription factors in living human cells with the help of split ubiquitin system
Ngày tải lên :
29/09/2015, 13:01
... of the 69 (86%) known motifs and 53 of the 105 (50%) new motifs The TATA Box is the binding site for the TATA-binding protein (TBP) In some genes, the transcription initiation site consists of ... from these model organisms The accuracy and coverage of the interactions predicted depend on the quality of the original model organism protein and the ability to identify the human orthologs of ... Protein-protein interactions are crucial for the formation of complexes involved in the regulation of transcription Interactions of the human linker histone hH1 with the other core histone proteins, hH2A,...
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Tài liệu The Unix Family of Operating pdf
Ngày tải lên :
21/12/2013, 11:15
... Versions of Unix There are several versions of Unix Until a few years ago, there were two main versions: the line of Unix releases that started at AT&T (the latest is System V Release 4), and another ... keyboard and then press the RETURN key The lefthand column tells you what will happen After you try the commands, you’ll have a better idea of the ones you want to learn more about You can then get ... Our examples show the bash shell and the GNOME and KDE window environments We’ve chosen them because they’re popular and make good examples, not because we think they’re always the best.” If you...
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Tài liệu 77 Introduction to the TMS320 Family of Digital Signal Processors docx
Ngày tải lên :
25/12/2013, 06:16
... “opcode” of the instruction The opcode is the unique identifier of the instruction The next four bits, 1000, are the shift of the operand Then there is one bit (zero in this case) to signal that the ... simplified form the key features of the TMS320C30 The major parts of the DSP processor are the memory, the CPU, the peripherals, and the direct memory access (DMA) unit Each of these parts will ... ALU of the TMS320C30 The heart of the TMS320C30 is the CPU consisting, primarily, of the multiplier and the ALU The CPU configuration is shown in Fig 77.16 which depicts the multiplier and the...
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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt
Ngày tải lên :
18/02/2014, 18:20
... proliferator-activated receptors are members of the nuclear hormone receptor family, the largest family of transcription factors [14] Three distinct members of the PPAR subfamily have been reported: a, d ... modulating transcription factor activity, such as control of their synthesis or activity, including the inhibition of protein–protein interactions in the activating signaling cascade of the transcription ... activity of AP-1 and increased expression of Fos ⁄ Jun [30] Thus, AP-1 proteins have significant pathological roles in RA Other transcription factors Other transcription factors implicated in the pathogenesis...
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Tài liệu Báo cáo khoa học: The most C-terminal tri-glycine segment within the polyglycine stretch of the pea Toc75 transit peptide plays a critical role for targeting the protein to the chloroplast outer envelope membrane ppt
Ngày tải lên :
19/02/2014, 07:20
... frequently in these regions Together they account for 17% of residues within this stretch We replaced the critical tri-glycine within the pea Toc75 transit peptide with repeats of these three FEBS ... investigate the importance of certain residues within the polyglycine stretch for targeting specificity First, we aimed to test whether or not the entire polyglycine stretch within the residues 91–100 of ... targeting of Toc75 remains unknown In this report, we extended the analysis of the polyglycine stretch of the Toc75 transit peptide in order to better understand the targeting mechanism of the chloroplast...
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Tài liệu Báo cáo khoa học: Proteasome involvement in the degradation of the Gq family of Ga subunits pptx
Ngày tải lên :
20/02/2014, 03:20
... activation of Ga16 and Gaq subunits Therefore the lack of change in the rate of degradation cannot be due to lack of receptor-activation of the alpha subunits Given that the activation of G proteins ... Accordingly, the levels of Ga16 decay were the same in the presence or absence of carbachol (Fig 4C) in cells expressing the M2R Also the activated mutant of A B C D Fig Functional assay of the Ga ... forms of Gaq and Ga16 have the same behavior as the wild-type forms argues in favor of the need of other proteins to stabilize the proteins in the membrane The interaction of Ga subunits with Gbc...
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Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
Ngày tải lên :
21/02/2014, 15:20
... differences in the C-terminal part of the enzyme (Fig 2A) where 13 of 44 amino acids are different Therefore, chimeric enzymes with the N-terminal part from the human and the C-terminal part from the murine ... were constructed by use of the EcoRV and SacI sites in the cDNA and were then used to transform 293-cells The region responsible for the different degree of association of the human and murine enzyme ... about one tenth of the specific activity of the oligomeric complex [15] For the recombinant human enzyme the nonassociated form also appeared to be dimers of the 138 kDa subunit, since their Mr was...
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Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
Ngày tải lên :
08/03/2014, 22:20
... parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest known branch of the cathepsin B family [23] Its phylogeny confirms that the ... concentrating the allergen according to the isoelectric point (pI) of the molecule A pH gradient from to 11 was established for the characterization of Phl p After completion of the run, the individual ... further explore the biochemical function of group I allergens Expression of glycosylated and nonglycosylated rPhl p reveals differing stability of these allergens The expression of rPhl p in the...
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Báo cáo khoa học: The ROQUIN family of proteins localizes to stress granules via the ROQ domain and binds target mRNAs pdf
Ngày tải lên :
15/03/2014, 11:20
... above the relevant lanes, and the absence of any added protein is indicated by ()) The position of the free RNA and of the loading slots is indicated to the right of each gel A B abolished by the ... granules is shared by the MNAB protein encoded by the other member of the mammalian Rc3h gene family, Rc3h2 Stress granule localization is likely to be critical for the function of these proteins, ... series of GFP fusions with the various motifs of Roquin, and by selectively deleting domains from the full-length protein, we determined that the ROQ domain in the N-terminus of the protein is the...
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Báo cáo khoa học: A structural overview of the PDI family of proteins docx
Ngày tải lên :
15/03/2014, 23:20
... Specifically, the side chains of Leu343 and Trp347, which are part of the linker between the b¢ and a¢ domains, are inserted into the PDI family of proteins hydrophobic cavity of the b¢ domain The binding ... Representation of the full-length ERp57 structure with the region recognized as the x-linker colored in red (B) The N-terminal part of the x-linker folds against the b¢ domain in the structures of the ERp57 ... conformations in the structures of the isolated b¢ domain and full-length proteins In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turns back to contact the...
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Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot
Ngày tải lên :
16/03/2014, 02:20
... they in the EPHOPT complex (Fig 3) In addition, the backbone atoms of the Trp– Table Kinetic data The upper panel shows the effect of varying amino acids at the +2 position of the substrate The ... flexibility of these two regions, several other kinase resi˚ dues within A of the EPHOPT substrate are also shown These residues are clearly fixed in their orientation regardless of whether substrate ... especially the b1–b2 loop (G loop) and aC regions Crystallization of the complex between the EphA3 kinase domain and the EPHOPT peptide did not result in the full ordering of the AL; instead, the N-terminal...
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Báo cáo khoa học: "ON THE INTONATION OF MONO- AND DI-SYLLABIC WORDS WITHIN THE DISCOURSE FRAMEWORK OF CONVERSATIONAL GAMES ON THE INTONATION OF MONO- AND DI-SYLLABIC WORDS WITHIN THE DISCOURSE FRAMEWORK OF CONVERSATIONAL GAMES " pot
Ngày tải lên :
17/03/2014, 08:20
... and has to tell another person how to draw the path onto a similar map Neither participant can see the other's map The nature of the map task is such that from the conversations the speaker's intentions ... moves within themselves: right, okay, yes, no, mmhmm, and nhhuh In addition, I am categorizing the cases where these words form part of a move They typically surface as of the 12 moves in the games ... elicited moves) segment The data yield the following results s: 42% of rises (5 of 11) appear as connecting moves, 30% of levels (13 of 44) as continuing moves, and 69% of falls (9 of 13) as segmenting...
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Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx
Ngày tải lên :
23/03/2014, 12:20
... mechanisms [42,44] The study of the effect of pH on enzyme inactivation allows the calculation of the pKa of the His176 side chain involved in the inactivation reaction The rate of inactivation ... nitrogen atoms of the backbone of the residue preceding the catalytic Cys The conservation of this interaction is suggestive of its Fig The nal model of the clostripain catalytic domain The ribbon ... nonapeptide The nal residue of the resultant a-chain, Arg181, and the rst residue of the b-chain, Ala191, are also labelled (italics) The magenta colouring towards the bottom of the gure marks the position...
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