specific biological activity of lacquer urushi polysaccharides

... the biological activity and to study the effect of reducing the chain length of peptides on Ó FEBS 2004 Design of peptides for T-cell adhesion inhibition (Eur J Biochem 271) 2879 biological activity, ... bonds between NH of Thr5 and CO of Arg2, as well as NH of Arg2 and CO of Thr5 Structure of cyclic hexapeptides – cYT peptide The NMR data of the cYT peptide were indicative of its flexible nature ... conformation of the peptide are important for biological activity Truncation of the cVY peptide to the hexapeptide cYT resulted in drastically reduced biological activity suggesting the lack of stable...

Ngày tải lên: 19/02/2014, 13:20

... presence of minute amounts of endotoxins The comparison of LPS and MfGl-II in the LAL assay shows, as expected, activity of LPS in the range of down to 10 pgÆmL)1, whereas MfGl-II has only activity ... determination of TNF-a in duplicate at two different dilutions The data are representative of three independent measurements LAL assay The LAL test is based on the property of lysates of amebocytes of the ... added Endotoxin activity determination by the chromogenic Limulus test Endotoxin activity of the glycolipids was determined by a quantitative kinetic assay [27] based on the reactivity of Gram-negative...

Ngày tải lên: 21/02/2014, 00:20

... 25-deoxyecysone Inhibition of 3-dehydroecdysone synthesis was not measured, but this is a minor secretory product of Carcinus YO [11] Loss of sensitivity of YO to the inhibitory influence of crude SG extracts ... The reduction of biological activity was also noted when the influence of MIH and CHH upon GMP levels in YO was considered For Carcinus, cGMP seems to be a particularly important part of the MIH ... YO, levels of cGMP were similar in each YO of individual crabs Fig Effects of MIH and CHH upon ecdysteroid synthesis by YO in vitro Upper graph shows the moult stage dependent inhibition of ecdysteroid...

Ngày tải lên: 21/02/2014, 00:20

... [17] Upon transfection of five cell types with the intact pTTV, DpnI-resistant (i.e replicating) DNA was detected However, rescued forms of the TTV genome Biological activity of a full-length TTV ... molecular and cell biological studies of this peculiar human virus For this purpose, we have cloned and sequenced the full-length genome of TTV genotype 6, and used it to detect the key biological functions ... product of approximately 3.7 kb was detected; data not shown) The circularization was further confirmed by digestion of the DNA samples with pairs of restriction enzymes that cut on both sides of the...

Ngày tải lên: 16/03/2014, 05:20

... down-regulation of the nociceptin/orphanin FQ receptor to be the mechanism of biological activity of compounds I and II The antisense pretreatment remained without any influence on the basal beating rate of ... the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of I or II on day and after seeding The basal beating rate of the CM on day of culture was 191 ... the mean of 30–40 cells or cell cluster ± SEM Fig Influence of 10)5 M nociception on the beating rate of CM measured on day of culture without and with pretreatment with either 0.2 lM of II or...

Ngày tải lên: 23/03/2014, 13:20

... vicinity of cultured cells provoked a wave of calcium in the cytoplasm of astrocytes (Fig 3A) while ejection of culture medium alone had no effect (Fig 3B) Comparison of the amplitude of the response ... conformation of peptides without altering the side chain motifs that are often involved in their biological activity [13,14] In several cases, cyclization has been found to enhance the potency of peptides ... of a potent endozepine antagonist (Eur J Biochem 268) 6049 Fig RP-HPLC monitoring of N- and C-terminal deprotections and on-resin cyclization of the linear precursor of cyclo128 OP Aliquots of...

Ngày tải lên: 24/03/2014, 04:21

... in vitro evaluation of their biological activity, using ALP activity as a marker, revealed alterated effects for mutants of epitope and epitope as well But only alterations of epitope lead to ... (O) within central areas of the scaffold In the present study the inhibitory activity of the BMP-2 double mutant A34D/D53A could be demonstrated in vivo by inhibition of a specific osteoinductive ... Origin of the proteins The developement and expression of the utilized proteins in a bacterial expression system was performed by the department of physiologic chemistry II, University of Würzburg,...

Ngày tải lên: 11/08/2014, 20:20

... 1, 2.5 lg of GST-ExoS(wt); lane 2, 1.25 lg of GST-ExoS(wt); lane 3, 0.75 lg of GST-ExoS(wt); lane 4, 2.5 lg of GST-ExoS(L426A); lane 5, 1.25 lg of GST-ExoS(L426A); lane 6, 0.75 lg of GST-ExoS(L426A); ... 2.5 lg of GST-ExoS(D427A); lane 8, 1.25 lg of GST-ExoS(D427A); lane 9, 0.75 lg of GST-ExoS(D427A); lane 10, 2.5 lg of GST-ExoS(L428A); lane 11, 1.25 lg of GST-ExoS(L428A); lane 12, 0.75 lg of GST-ExoS(L428A); ... antibodies It should be noted that we did not investigate binding of different 14-3-3 isoforms or the specificity of different 14-3-3 isoform binding in this study, as we used a pan-14-3-3 antibody...

Ngày tải lên: 19/02/2014, 08:20

... a remarkable increase of scattered light, of the order of 10 times that of the pure solvent, indicating the occurrence of some aggregation An additional fourfold increase of the scattered light ... radius of b-CN(1–25)0P is 150 nm versus the 60 nm of b-CN(1–25)4P However, the relative concentration of aggregates is about 2.5% that of b-CN(1–25)4P, regardless of the presence or absence of Ca2+ ... correspond to those of the dose biological response (Fig 3), showing that at mmolÆL)1 Ca2+, where the maximal biological activity is reached, there is the highest concentration of CPP aggregates...

Ngày tải lên: 16/03/2014, 05:20

... Variation of this composition such as a reduction of the number of charges or the number of acyl chains, a change in their distribution, or degree of saturation, results in a dramatic reduction of biological ... Chemical synthesis of a structure and immunoreactivity of the lipopolysaccharbiosynthetic precursor of lipid A Bull Chem Soc Jpn 60, ide of the deep rough mutant I-69 Rd- ⁄ b+ of Haemo2197–2204 ... molecular shape of the composing molecules [5] We have previously shown that a peculiar molecular shape of the lipid A portion of endotoxin is a prerequisite for the expression of endotoxic activity...

Ngày tải lên: 23/03/2014, 13:20

... observations provide strong evidence in support of the importance of specific mp-cp interactions in natural infections of maize Specific binding of MP and CP has been demonstrated in vitro and ... were assessed in terms of infectivity and symptomatology, and evidence of biologically-important specific interactions between the MSV MP and CP is presented Results Viability of chimaeric genomes ... aim of identifying host specificity determinants Although none of these chimaeras were able to systemically infect host plants of either parental virus, the study demonstrated the importance of...

Ngày tải lên: 20/06/2014, 01:20

... observation that deletion of Nlinked glycosylation in gD (YP11mu) does not affect biological activities of CHV gD Although the role of other two potential N-linked glycosylation sites of CHV gD remains ... this 12 bp deletion was specific for YP11mu strain Expression of CHV gDs in COS-7 cells: Expression of CHV gD (YP11mu) in COS-7 cells was examined by IFA using MAbs All of the MAbs against CHV ... pME-fgD-transfected cells, any specific band was not detected (Fig 2A lane3) Expression of CHV gDs in insect cells: Expression of CHV gD (YP11mu) in Sf9 cells was examined by IFA using MAbs All of the MAbs against...

Ngày tải lên: 07/08/2014, 11:21

... For oligoresveratrol, that activity as hydroxyl radical scavenger was due to the existence of phenol ring, stability of molecular structure and existence of double bonds of olefinic unit Phenol ... cytotoxic activity of the samples against HeLa-S3 cell measured as LC50 were provided in Table HeLa-S3, a continuous cell line that lived as adherent cell, is a cell derivate of ephythell cell of human ... investigation of cytotoxic activity of the samples was held against Raji cell (Table 4), the cell that resembles lymphoblast cell found by R.J.V Pulvertaft (1963) from Burkitt’s lymphoma at the left of...

Ngày tải lên: 07/08/2014, 14:20

... diversity of collagen families makes development of a comprehensive, single model system challenging A system capable of emulating specific variables, such as the structure and biological activity, of ... TGase Identification of the reactive glutamyl residue in APQQEA substrate Specific recognition of EDGFFKI by tissue TGase In situ localization of endogenous transglutaminase activity using EDGFFKI ... conformation- and sequence -specific, the absence of which resulted in a marked loss of cell recognition Furthermore, a specific sequence spanning residues 2800-2807 of human fibrillin-1 (EDGFFKI)...

Ngày tải lên: 12/09/2015, 08:20

... progression in eight different types of cancer, and evaluated the anticancer activity of the PR domain against human hepatoma HuH7 cells through both direct administration of recombinant His6-tagged PR ... was performed using GraphPad InStat (GraphPad Software, San Diego, California, USA) Anticancer activity of PR domain by cDNA transfection The cloning of the PR domain (residues 13-193) has been ... respectively Western blot of PR domain The expression of the PR domain was examined after 24 hr of transfection with pcDNA/HisMax/PR and the empty vector, respectively Supernatants (30 g) of the cell lysates...

Ngày tải lên: 25/10/2012, 11:15

... organization of nitrifying bacterial populations and the in situ activity of these populations within the biofilms was discussed Microsensors For analysis of microbial structure and function (activity) of ... steady-state microprofiles of O2, NH4+, NO2-, and NO3- in the autotrophic nitrifying biofilm (B) The distribution and magnitude of the estimated specific rates of net consumption and production of NH4+, ... performed Fixation and Cryosectioning of Biofilm Samples Biofilm samples were taken at regular time intervals during the periods of the biofilm development The biofilm samples were fixed with freshly...

Ngày tải lên: 05/09/2013, 08:40

... complexity of cell wall Silver ion (Ag+) is a bactericidal agent (Pedahzur et al., 1995) The binding of Ag+ and respiratory enzymes initiates changes in permeability of plasma membranes of bacteria ... standard deviation of independent triplicates - 49 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Effect of Ag content in photocatalysts Bactericidal activities of the three photocatalysts ... deviation of independent triplicates - 50 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Increasing the concentration of photocatalysts did not increase the bactericidal activity...

Ngày tải lên: 05/09/2013, 09:08

... weeks of pre-incubation period, indicating the availability and activities of microorganism in river water However, as shown in Fig 3, there was no considerable change of DOC in the mixture of DOM ... of DOM of WWTP effluents and pre-incubated river water within the first week of incubation Slight DOC reduction (14%) was observed after one month of incubation In contrast to the results of the ... treatment of wastewater by activated sludge process has shifted the DOM composition to more biologically recalcitrant compounds in terms of zinc complexation On the other hand, a further examination of...

Ngày tải lên: 05/09/2013, 10:15

... structure of the available inhibitors of cathepsin D [19], a lysosomal protease of mammalian cells, and chalcones and phenothiazines have been assayed as inhibitors of falcipain-2 [20,21] Inhibition of ... Discussion Inhibition of proteases by MA It has been previously proposed that MA may inhibit the activity of proteases of T gondii [14] and HIV [12,31] To ascertain the inhibition range of MA on the different ... proteolytic hydrolysis of haemoglobin as a source of amino acids constitutes one of the essential processes which take place along the intra-erythrocytic stage of the parasite Degradation of haemoglobin...

Ngày tải lên: 14/02/2014, 14:20

... hydrolytic activity towards ATP (Fig 1A) The binding of a magnesium cofactor has long been established as essential for the activity of canonical GTP-binding proteins [29,31] The precise role of Mg2+ ... determine the requirement for magnesium of the GTPase activity of TorD, the reaction was performed in varying amounts of MgCl2 (Fig 1B) The GTPase activity of TorD without added MgCl2 is negligible, ... co-ordination of the two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12] The concentration of GTP in the cytoplasm of...

Ngày tải lên: 16/02/2014, 09:20