species cell lines and animal breeds

... ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by quantitative RT-PCR with U6 as an internal standard miR-22...

Ngày tải lên: 22/03/2014, 21:20

... than 100 molecular and cellular impairments, such as inadequate angiogenesis [7], impaired innervation [8], impaired cellular migration [9] and abnormal keratinocyte activation and differentiation[10] ... primary cell cultures originating from actual patients and establishing cellular tests that can help evaluate potential therapy on target wound cells In this report, we demonstrate that cells grown ... Figure 1) Cells from location B are those surgically removed and cells from location C are the cells left behind after surgery One piece of the debrided tissue was sent for routine pathology and other...

Ngày tải lên: 18/06/2014, 15:20

... AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 fold more resistant to mitoxantrone than more mature HL-60 and U937 cells, and ... AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin selectively induced apoptosis in KG1a and Kasumi1 cell lines, as well as in primary CD34+ AML cells, ... suppression of cell growth of human T -cell leukemia virus type Iinfected T -cell lines and primary adult T -cell leukemia cells Int J Cancer 2006, 118:765-772 21 Kunnumakkara AB, Anand P, Aggarwal...

Ngày tải lên: 18/06/2014, 19:20

... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... MOVCAR cell lines showed a clear expression of intracellular Muc16 and only minimal presence of Muc16 on the cell surface (Figs 6a–d) Similar results were obtained with both the 618F and 653F ... epithelial ovarian tumor cell lines OVCAR-3, SKOV-3, and CAOV-3 were purchased from ATCC RT-PCR Total RNA was isolated from MOVCAR cell lines using the Qiagen RNeasy® Mini kit and μg of total RNA...

Ngày tải lên: 20/06/2014, 07:20

Ngày tải lên: 13/08/2014, 01:20

... the cell growth of HTLV-1-infected T -cell lines and primary ATL cells Next we examined the effect of AG490 on the growth of HTLV-1-infected T -cell lines and primary ATL cells HTLV1-infected T -cell ... AG490-treated cells Thus, AG490 is both anti-proliferative and pro-apoptotic in HTLV-1infected T -cell lines Figure and primary ATL cells AG490 reduces cell growth of HTLV-1-infected T -cell lines AG490 ... HTLV-1-infected T -cell lines Figure HTLV-1-infected T -cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat...

Ngày tải lên: 13/08/2014, 09:21

... (JNK1/2/3) and p38 isoforms (α, β, γ and δ) Usually, ERK1/2 is involved in cell proliferation and differentiation, while JNK and p38 cascades are activated by cellular stresses However, depending on cell ... p38 siRNA and control siRNA-A were purchased from Santa Cruz Biotechnology 2.2 Cell lines MCF-7, MDA-MB-231 and BT-474 breast cancer cell lines as well as MCF10A normal breast epithelial cell line ... MCF-7 and MDA-MB-231 breast cancer cells (Colin et al., 2010) In addition, the cytotoxicity of 15d-PGJ2 in 786-O, Caki-2 and ACHN human renal cancer cell lines was mediated in caspasedependent and...

Ngày tải lên: 10/09/2015, 09:09

... Molecular Probes Homeostasis of ROS in q0 cells (Eur J Biochem 271) 3647 Cell lines and culture conditions The q+ wild-type osteosarcoma cells (143B) and the q0 cells derived from 143B were a gift ... decreased in bone and muscle q0 cells compared with their parental q+ lines Fig GPx and GR activities in q+ and q0 cells from osteosarcoma (bone), rhabdomyosarcoma (muscle) and lung carcinoma ... Antioxidant defences of q0 cell lines GSH-GSSG and GR We measured GSH and GSSG in exponentially growing cells, as GSH content changes in the growth and lag phases [51] In all q0 cells studied, GSH was...

Ngày tải lên: 16/03/2014, 18:20

... tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos, MDAMB231, MCF7, HeLa and ... primary HFF cells and different tumor cell lines Similar to protein levels, both Cdt1 and geminin mRNA was markedly increased in all the tumor cell lines tested compared with the primary HFF cells ... geminin and actin (as loading control) are shown in serum deprived NIH3T3 cells, and and 12 h following serum re-addition, and compared with mRNA levels in proliferating cells Both Cdt1 and geminin...

Ngày tải lên: 07/03/2014, 16:20

... IL-2R subunits and HLA glycoproteins in T leukemia and lymphoma cell lines (A) Representative FRET efficiency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell basis, using ... T cell lines by flow cytometry The IL-2R a and cc chains were found constitutively expressed in several (6–10) thousands of copies in all cell lines, except in MT-1, which is deficient in a and ... that of the a and c chains on all the four T cell types (‡ 105 per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface...

Ngày tải lên: 17/03/2014, 23:20

... against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results ... 48 Time (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell...

Ngày tải lên: 23/03/2014, 07:20

... propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; primate and canine cell lines may also be used [5] It has been ... plasmids and clonal isolation, which makes setting-up an inducible cell line a protracted (at least weeks if one buys cell lines prepared for the final round, or 12 weeks if one starts from scratch) and ... is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow cytometry 293 cells (ATCC CRL-1573), a transformed cell line derived from human embryonic kidney, were...

Ngày tải lên: 23/03/2014, 09:21

... cell lines Although the mammalian cell culture system grows slowly, with lower cell density and lower production rate, compared to that of other cell lines (Chun et al., 2001), mammalian cell lines, ... immunoglobulins, and thrombolytics (Chun et al., 2001) ANIMAL CELL TECHNOLOGY Animal cell lines  CHO (Chinese hamster ovary) 162 Kỷ yếu hội nghị Khoa học Môi trường Công nghệ sinh học năm 2011 Mammalian cell ... disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins  Due to low yield and complicated and costly cultivation, therefore animal cells...

Ngày tải lên: 28/03/2014, 09:20

... intra- and extracellular volumes probably occurs, and can lead to chemical imbalances Depending on the ratio of intra- and extracellular volume, the composition of the extracellular medium, and ... sample, cells vary in size and shape, as well as in the dielectric and conductive properties of cellular components, such that the critical field and membrane relaxation time differ from cell to cell ... below the equilibrium value N, = Vcellcext (3740) Here N, is the number of molecules taken up by a single cell, Vcell is the cell volume, and c,,, is the extracellular concentration in a large...

Ngày tải lên: 11/04/2014, 09:32

... metformin, from DM, HS and CN for the care of melanoma cell lines and cell viability studies and from BC-A for the optimization and conduct of cell viability, cell cycle, apoptosis and phosphoflow studies: ... of μM vemurafenib and 10 mM metformin of all melanoma cell lines tested The green cell lines harbor BRAFV600E, red shows NRASQ61 mutant cell lines, and black the wild type cell line M257 Propidium ... combination on cell proliferation and viability a) Example of growth curves of BRAFV600E and NRASQ61 mutant melanoma cell lines BRAFV600E cell lines included M263 (synergistic) and M308 (antagonistic)...

Ngày tải lên: 18/06/2014, 19:20

... Page of 20 Methods Cell Lines and Cell Culture Tumor cell lines were obtained from the American Type Culture Collection (ATCC) or were gifted to the Epstein laboratory Tumor cell line authenticity ... fibroblast cell lines (induction negative control), and cytokine-induced MDSC (GM-CSF + IL-6, suppressive control) were run in parallel for comparison Irradiated tumor cell lines and T cell depleted ... Induction and functional characterization of canonical CD33+ MDSC by human tumor cell lines A, HNSCC-induced MDSC inhibit autologous T cell proliferation and IFNg production A subset of HNSCC cell lines...

Ngày tải lên: 18/06/2014, 19:20

... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... from screening cell lines in cellular proliferation assays and from cell cycle analyses Cell lines were classified into one of three categories based on the time when the majority of cells contained ... on both time of response and degree of cell death 20/91 (22%) cell lines were designated sensitive and 39/91 (43%) cell lines were designated resistant (where sensitive and resistant is defined...

Ngày tải lên: 18/06/2014, 22:20

... I and type II exhibit different transformation and outgrowth potentials [33] Jijoye and P3HR1 are EBV-producing cell lines, Akata cells can be induced to produce EBV, and Raji and Namalwa cells ... lytic EBV genes in BL cell lines were lower than in B95.8, but among the BL cell lines tested, transcription was high in producer cell lines (permissive) such as Jijoye and P3HR1 and low (up to 10-fold) ... in the selected BL cell lines than in B95.8 BZLF1 transcription ratios varied between 0.1 and 0.6 (a sixfold range) and were very low in all BL cell lines In Namalwa and Raji cells, transcription...

Ngày tải lên: 20/06/2014, 01:20