species cell lines and animal breeds
Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by quantitative RT-PCR with U6 as an internal standard miR-22...
- 11
- 237
- 0
báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... than 100 molecular and cellular impairments, such as inadequate angiogenesis [7], impaired innervation [8], impaired cellular migration [9] and abnormal keratinocyte activation and differentiation[10] ... primary cell cultures originating from actual patients and establishing cellular tests that can help evaluate potential therapy on target wound cells In this report, we demonstrate that cells grown ... Figure 1) Cells from location B are those surgically removed and cells from location C are the cells left behind after surgery One piece of the debrided tissue was sent for routine pathology and other...
- 9
- 487
- 0
Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... AML cells are 10-15-fold more resistant to DNR than CD34- AML cells [8] CD34+ KG1a and TF-1 AML cell lines are 30-40 fold more resistant to mitoxantrone than more mature HL-60 and U937 cells, and ... AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin selectively induced apoptosis in KG1a and Kasumi1 cell lines, as well as in primary CD34+ AML cells, ... suppression of cell growth of human T -cell leukemia virus type Iinfected T -cell lines and primary adult T -cell leukemia cells Int J Cancer 2006, 118:765-772 21 Kunnumakkara AB, Anand P, Aggarwal...
- 15
- 415
- 0
báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... MOVCAR cell lines showed a clear expression of intracellular Muc16 and only minimal presence of Muc16 on the cell surface (Figs 6a–d) Similar results were obtained with both the 618F and 653F ... epithelial ovarian tumor cell lines OVCAR-3, SKOV-3, and CAOV-3 were purchased from ATCC RT-PCR Total RNA was isolated from MOVCAR cell lines using the Qiagen RNeasy® Mini kit and μg of total RNA...
- 7
- 430
- 0
Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... the cell growth of HTLV-1-infected T -cell lines and primary ATL cells Next we examined the effect of AG490 on the growth of HTLV-1-infected T -cell lines and primary ATL cells HTLV1-infected T -cell ... AG490-treated cells Thus, AG490 is both anti-proliferative and pro-apoptotic in HTLV-1infected T -cell lines Figure and primary ATL cells AG490 reduces cell growth of HTLV-1-infected T -cell lines AG490 ... HTLV-1-infected T -cell lines Figure HTLV-1-infected T -cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat...
- 10
- 271
- 0
Evaluation of thymoquinone for cytotoxic activity against human breast cancer cell lines and tumor xenograft in nude mice
... (JNK1/2/3) and p38 isoforms (α, β, γ and δ) Usually, ERK1/2 is involved in cell proliferation and differentiation, while JNK and p38 cascades are activated by cellular stresses However, depending on cell ... p38 siRNA and control siRNA-A were purchased from Santa Cruz Biotechnology 2.2 Cell lines MCF-7, MDA-MB-231 and BT-474 breast cancer cell lines as well as MCF10A normal breast epithelial cell line ... MCF-7 and MDA-MB-231 breast cancer cells (Colin et al., 2010) In addition, the cytotoxicity of 15d-PGJ2 in 786-O, Caki-2 and ACHN human renal cancer cell lines was mediated in caspasedependent and...
- 176
- 386
- 0
Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... Molecular Probes Homeostasis of ROS in q0 cells (Eur J Biochem 271) 3647 Cell lines and culture conditions The q+ wild-type osteosarcoma cells (143B) and the q0 cells derived from 143B were a gift ... decreased in bone and muscle q0 cells compared with their parental q+ lines Fig GPx and GR activities in q+ and q0 cells from osteosarcoma (bone), rhabdomyosarcoma (muscle) and lung carcinoma ... Antioxidant defences of q0 cell lines GSH-GSSG and GR We measured GSH and GSSG in exponentially growing cells, as GSH content changes in the growth and lag phases [51] In all q0 cells studied, GSH was...
- 11
- 412
- 0
Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos, MDAMB231, MCF7, HeLa and ... primary HFF cells and different tumor cell lines Similar to protein levels, both Cdt1 and geminin mRNA was markedly increased in all the tumor cell lines tested compared with the primary HFF cells ... geminin and actin (as loading control) are shown in serum deprived NIH3T3 cells, and and 12 h following serum re-addition, and compared with mRNA levels in proliferating cells Both Cdt1 and geminin...
- 11
- 485
- 0
Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... IL-2R subunits and HLA glycoproteins in T leukemia and lymphoma cell lines (A) Representative FRET efficiency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell basis, using ... T cell lines by flow cytometry The IL-2R a and cc chains were found constitutively expressed in several (6–10) thousands of copies in all cell lines, except in MT-1, which is deficient in a and ... that of the a and c chains on all the four T cell types (‡ 105 per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface...
- 10
- 499
- 0
Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results ... 48 Time (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell...
- 9
- 331
- 0
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... propagated in 1% of initially transfected cells [4] EBV plasmid replication has been demonstrated for a large variety of human cell lines; primate and canine cell lines may also be used [5] It has been ... plasmids and clonal isolation, which makes setting-up an inducible cell line a protracted (at least weeks if one buys cell lines prepared for the final round, or 12 weeks if one starts from scratch) and ... is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow cytometry 293 cells (ATCC CRL-1573), a transformed cell line derived from human embryonic kidney, were...
- 8
- 331
- 0
Báo cáo " RECOMBINANT PROTEIN PRODUCTION: EXPRESSION SYSTEMS AND ANIMAL CELL TECHNOLOGY " potx
... cell lines Although the mammalian cell culture system grows slowly, with lower cell density and lower production rate, compared to that of other cell lines (Chun et al., 2001), mammalian cell lines, ... immunoglobulins, and thrombolytics (Chun et al., 2001) ANIMAL CELL TECHNOLOGY Animal cell lines CHO (Chinese hamster ovary) 162 Kỷ yếu hội nghị Khoa học Môi trường Công nghệ sinh học năm 2011 Mammalian cell ... disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins Due to low yield and complicated and costly cultivation, therefore animal cells...
- 7
- 466
- 0
animal cell electroporation and electrofusion protocols
... intra- and extracellular volumes probably occurs, and can lead to chemical imbalances Depending on the ratio of intra- and extracellular volume, the composition of the extracellular medium, and ... sample, cells vary in size and shape, as well as in the dielectric and conductive properties of cellular components, such that the critical field and membrane relaxation time differ from cell to cell ... below the equilibrium value N, = Vcellcext (3740) Here N, is the number of molecules taken up by a single cell, Vcell is the cell volume, and c,,, is the extracellular concentration in a large...
- 358
- 334
- 0
Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... metformin, from DM, HS and CN for the care of melanoma cell lines and cell viability studies and from BC-A for the optimization and conduct of cell viability, cell cycle, apoptosis and phosphoflow studies: ... of μM vemurafenib and 10 mM metformin of all melanoma cell lines tested The green cell lines harbor BRAFV600E, red shows NRASQ61 mutant cell lines, and black the wild type cell line M257 Propidium ... combination on cell proliferation and viability a) Example of growth curves of BRAFV600E and NRASQ61 mutant melanoma cell lines BRAFV600E cell lines included M263 (synergistic) and M308 (antagonistic)...
- 13
- 518
- 0
Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... Page of 20 Methods Cell Lines and Cell Culture Tumor cell lines were obtained from the American Type Culture Collection (ATCC) or were gifted to the Epstein laboratory Tumor cell line authenticity ... fibroblast cell lines (induction negative control), and cytokine-induced MDSC (GM-CSF + IL-6, suppressive control) were run in parallel for comparison Irradiated tumor cell lines and T cell depleted ... Induction and functional characterization of canonical CD33+ MDSC by human tumor cell lines A, HNSCC-induced MDSC inhibit autologous T cell proliferation and IFNg production A subset of HNSCC cell lines...
- 20
- 575
- 0
Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... from screening cell lines in cellular proliferation assays and from cell cycle analyses Cell lines were classified into one of three categories based on the time when the majority of cells contained ... on both time of response and degree of cell death 20/91 (22%) cell lines were designated sensitive and 39/91 (43%) cell lines were designated resistant (where sensitive and resistant is defined...
- 10
- 618
- 0
Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx
... I and type II exhibit different transformation and outgrowth potentials [33] Jijoye and P3HR1 are EBV-producing cell lines, Akata cells can be induced to produce EBV, and Raji and Namalwa cells ... lytic EBV genes in BL cell lines were lower than in B95.8, but among the BL cell lines tested, transcription was high in producer cell lines (permissive) such as Jijoye and P3HR1 and low (up to 10-fold) ... in the selected BL cell lines than in B95.8 BZLF1 transcription ratios varied between 0.1 and 0.6 (a sixfold range) and were very low in all BL cell lines In Namalwa and Raji cells, transcription...
- 15
- 426
- 0
- head and neck cancer cell lines hpv
- what does the fossil record tell us about plant and animal species
- karyotype and fluorescent in situ hybridization analysis of human embryonic stem cell and induced pluripotent stem cell lines
- exotic species and fungi interactions with fungal plant and animal communities
- 10 available cell lines and models
- inputs from cell lines and primary tumors of astrocytic origin
- dhea antiviral properties in cell cultures and animal models
- species cell selection and justification
- translational stem cell research and animal use examining ethical issues and opportunities
- macrophages macrophage cell lines and human and mouse b cells
- plasmids cell lines and viruses
- samples cell lines and dna extraction
- plasmids cell lines and reagents
- animals cell lines and antibodies
- cell lines and growth condition
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25