inputs from cell lines and primary tumors of astrocytic origin

... suppression of cell growth of human T -cell leukemia virus type Iinfected T -cell lines and primary adult T -cell leukemia cells Int J Cancer 2006, 118:765-772 21 Kunnumakkara AB, Anand P, Aggarwal ... primary CD34 AML cells (A) Primary CD34+ cells isolated from BMMCs or PBMCs of AML patients and healthy donors were isolated and subjected to flow cytometry to determine the purity of CD34+ cells ... USA) Cell lines, primary samples, and cell culture KG1a and Kasumi-1 cell lines were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ) (Braunschweig, Germany) and...

Ngày tải lên: 18/06/2014, 19:20

Ngày tải lên: 13/08/2014, 01:20

... the cell growth of HTLV-1-infected T -cell lines and primary ATL cells Next we examined the effect of AG490 on the growth of HTLV-1-infected T -cell lines and primary ATL cells HTLV1-infected T -cell ... in HTLV-1-infected T -cell lines and primary ATL cells (A and B) HTLV-1-infected T -cell lines were treated with increasing concentrations of AG490 for 24 h (C) Primary ATL cells were treated with ... HTLV-1-infected T -cell lines Figure HTLV-1-infected T -cell lines and primary ATL cells AG490 inhibits constitutive activation of Jak and Stat in AG490 inhibits constitutive activation of Jak and Stat...

Ngày tải lên: 13/08/2014, 09:21

... Figure 1) Cells from location B are those surgically removed and cells from location C are the cells left behind after surgery One piece of the debrided tissue was sent for routine pathology and other ... as measurement of growth factor production and response, expression of cell cycle proteins, and cell morphology, hold a piece of the puzzle as to why certain wounds not heal Part of the challenge ... containing cells strains derived from chronic wounds By using the methodology as described here, researchers can produce their own cell lines from chronic wounds in a standard fashion These cell lines...

Ngày tải lên: 18/06/2014, 15:20

... epithelial ovarian tumor cell lines OVCAR-3, SKOV-3, and CAOV-3 were purchased from ATCC RT-PCR Total RNA was isolated from MOVCAR cell lines using the Qiagen RNeasy® Mini kit and μg of total RNA was ... background fluorescence of the isotype control, our results for all MOVCAR cell lines showed a clear expression of intracellular Muc16 and only minimal presence of Muc16 on the cell surface (Figs ... Specificity of 618F for human MUC16 Specificity of 618F for human MUC16 (A) Purity of human MUC16 isolated from spent media of OVCAR-3 cells as determined by Coomassie Blue (1) and silver staining (2) of...

Ngày tải lên: 20/06/2014, 07:20

... Department of Biochemistry who, through the peculiarities and profundities of weekly seminar, have expanded the globe of my scientific exploration I thank every single member of the Clapp and Yang ... neurofibromas are slowly progressing, heterogeneous solid tumors comprised of Schwann cells, fibroblasts, vascular cells, and infiltrating hematopoietic cells, predominantly degranulating mast cells ... progression, and severity of NF1-associated MPD Mek-Erk Signaling in Mast Cells SCF regulates mast cell and hematopoietic progenitor cell cytopoiesis, proliferation, survival, and cytokine synthesis, and...

Ngày tải lên: 24/08/2014, 12:53

... The dose- and time-response curves of TQ treatment in several breast cancer cell lines and a normal breast cell line .46 Figure 3.1.2 Growth inhibition rate of the combination of TQ andchemotherapeutic ... isoforms (α, β, γ and δ) Usually, ERK1/2 is involved in cell proliferation and differentiation, while JNK and p38 cascades are activated by cellular stresses However, depending on cell lines and ... Objectives and overview of study 26 1.8.1 1.8.2 Objectives of study 26 Overview of study 27 MATERIALS AND METHODS 30 2.1 Chemicals and antibodies 30 2.2 Cell lines ...

Ngày tải lên: 10/09/2015, 09:09

... (t) and FS (t) are the earnings from term transformation of bank i and of the bond portfolio, respectively Combining (11) and (12), we see that a bank’s earnings from term transformation depend ... the course of time, depending on the current and past shape of the term structure Savings banks and cooperative banks are said to rely heavily on earnings from term transformation And, in fact, ... specific and regulatory effects are far more important For savings and cooperative banks, earnings from term transformation are an important source of interest income, and timely changes in earnings from...

Ngày tải lên: 16/02/2014, 06:20

... included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) and maintained in our ... regulation of cancer cell proliferation and growth For instance, let-7 can inhibit proliferation of lung and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c ... expression of miR-22 and ERa in breast cancer cell lines ERa is potentially involved in miR-22-mediated repression of ERa-positive breast cancer cell growth To investigate the role of ERa in miR-22-mediated...

Ngày tải lên: 22/03/2014, 21:20

... galectins [24]; (b) tested as ligand for surface receptors of different types of tumor cells; and (c) injected into circulation with monitoring of the time course of biodistribution The data set ... binding was determined for the B- and T-lymphoblastoid cells among the set of leukemia ⁄ lymphoma lines and to the mammary carcinoma cells among the carcinoma lines, when measuring staining FEBS ... instead of the neoglycoprotein and an inhibition of staining using glycoinhibitors (75 mM lactose and mg asialofetuinÆmL)1); (D) document lack of label ⁄ carrier protein-dependent binding and the...

Ngày tải lên: 30/03/2014, 16:20

Ngày tải lên: 04/05/2014, 14:27

... proposed and carried out projects of studies on Phytophthora diseases such as quick and low death of black pepper, quick death of durian, bud root of pineapple, wilt death of litchi, downy mildew of ... isolation from roots ranged from 15.0 to 26% 3.3 Isolation of Phytophthora caused root rot and bud rot of pineapple 3.3.1 Effect of media and sample types on Phytophthora isolation The result of experiment ... obtained from isolation of Phytophthora on black pepper, this fungus has been isolated in several plants (Table 8) 3.2 Isolation of Phytophthora from soil and root of several crops Table Effect of...

Ngày tải lên: 22/06/2014, 12:20

... analysed using Image Pro Plus Software to determine the average size of the foam cells The average cell sizes of the samples were determined from measurements of 30 different cells in the obtained micrograph ... of cells per unit volume and the average cell size An increase in the blowing agent concentration resulted in smaller, finer, and more uniform cells The decomposition of high concentrations of ... 13–14 Journal of Physical Science, Vol 20(1), 13–25, 2009 [ X ] phys = 2.4.3 17 (6) 2M c Number of cells per unit volume The number of cells per unit volume, the cell density, of the vulcanised...

Ngày tải lên: 07/08/2014, 14:20

... isolates from NAT/Crawford, WI/2006 and/ 2007 This is likely evidence for segment reassortment Phylogenetic analysis of the NSs and NSm genes from SI+ and I+ mosquitoes The multiple functions of the ... LACV SI+ and previously published isolates and between LACV NSm sequences from I+ and SI+ mosquitoes LACV NSm nucleotide and amino acid differences between LACV SI+ and I+ mosquitoes from one ... 18 of 27 Figure Molecular evolution of LACV: Comparison of segregating sites, haplotypes, and singletons between the three RNA segments of SI+ isolates and previously published isolates (7a) and...

Ngày tải lên: 12/08/2014, 04:20

... European Consensus Conference definition of ARDS [20] and relied upon the presence of PaO2/FiO2 (arterial partial pressure of oxygen/fraction of inspired oxygen) of less than or equal to 200 mm Hg, ... exposure and the development of incident ARDS Univariate descriptive statistics were generated for the ARDS and No ARDS groups Continuous data were summarized as mean and standard deviation, and the ... alterations in severity of illness Moreover, unlike the studies by Kahn and colleagues [10] and Gong and colleagues [9], ours is derived from a multi-institutional registry, and this underscores...

Ngày tải lên: 13/08/2014, 03:21

... numbers of offspring which develop rapidly and allow reproducibility of experimental settings and real-time analysis of development The transparency of the embryos together with the development of ... CNCCs and enables to visualize cells in pharyngeal arches (from Lister et al., 2006 ) (C) Lateral and ventral schematic drawings of cranial skeleton, the color code matches the originating cells ... Lrp5 and and thereby prevent the formation of the heterotrimeric complex of Wnt, Fz and Lrp5/6 (Semenov et al., 2001 and 2005; Li et al., 2002) 18 1.5 Canonical Wnt signaling in neural crest cells...

Ngày tải lên: 10/09/2015, 08:43

... level of Bubr1 and Mad2 after knockdown of p73 105 Fig 3.28: Different mechanism of action between p73 and Bubr1 and Mad2 in regulation of mitotic cell death 107 Fig 3.29: Knockdown of Bubr1 and ... 2.2.3.1 Cell death assays 2.2.3.2 Cell cycle and phospho-histone-H3 analysis 2.2.4 Immunofluorescence and confocal microscopy 2.2.5 Protein isolation and analysis 2.2.5.1 Total cell lysate 2.2.5.2 Cell ... the knockdown of Bubr1 and Mad2 in H1299 cells 103 Fig 3.26: Reduction in percentage of population of taxol treated cells undergoing apoptosis in p73, Bubr1 and Mad2 knockdown cells 104 Fig 3.27:...

Ngày tải lên: 13/09/2015, 21:21

... performance of unshielded wood pole transmission lines, and it includes the effect of weak links and non-linearities such as corona, wavefront distortion and the voltage-time behavior of the insulation ... 1.1.2 Review of previous work on calculating the lightning performance of distribution lines 1.1.3 Review of Liew’s work on dynamic model of impulse characteristics of concentrated earths ... Fig 6.2 Profiles of α as current rises and decays 76 Fig 6.3 Flowchart of the program to compute the earth resistivity 81 Fig 6.4 Sparking radius and the percentage of return...

Ngày tải lên: 08/11/2015, 17:33

... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro Results ... volume of the assays was mL [50] IC50 values were determined using linear regression analysis of the dose–response curves Cell culture and cytotoxicity assay HaCaT cells, HEL cells and HEp-2 cells...

Ngày tải lên: 23/03/2014, 07:20