special topic amino acids and polyamino acids peptides and proteins

amino acids, peptides and proteins in organic chemistry

Ngày tải lên : 02/04/2014, 15:59

... another by the number and sequence of the constituent amino acids Generally, a molecule comprised of few amino acids is called an oligopeptide and that with many amino acids is a polypeptide ... molecular interactions and thermodynamic control Thousands of proteins /peptides are assembled through the combination of only 20 amino acids (referred to as coded or proteinogenic amino acids) Post-translational ... Medicinal Chemistry of a-Hydroxy-b -Amino Acids 189 Zyta Ziora, Mariusz Skwarczynski, and Yoshiaki Kiso Introduction 189 a-Hydroxy-b -Amino Acids 189 a-Hydroxy-b -Amino Acids Occurring in Natural Products...

538
498
1

barrett - amino acids and peptides (cambridge, 2004)

Ngày tải lên : 04/06/2014, 16:05

... of polypeptides 1.8 ‘Non-protein amino acids , alias ‘non-proteinogenic amino acids or ‘non-coded amino acids 1.9 Coded amino acids, non-natural amino acids and peptides in nutrition and food ... Sources and roles of amino acids and peptides 1.2 Deﬁnitions 1.3 ‘Protein amino acids , alias ‘the coded amino acids 1.4 Nomenclature for ‘the protein amino acids , alias ‘the coded amino acids ... Detection of separated amino acids and peptides 4.18.1 Detection of amino acids and peptides separated by HPLC and by other liquid-based techniques 4.18.2 Detection of amino acids and peptides separated...

241
228
0

Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients

Ngày tải lên : 12/09/2015, 09:55

... system Some of the transmitters and receptors involved in pain transmission or modulation, especially peptides, excitatory amino acids (EAA) and inhibitory amino acids (IAA) are widely distributed ... using HPLC for amino acids in CSF was developed, which was then subsequently applied to quantitatively analyze all physiological amino acids especially the nine pain-related amino acids (asparagine, ... classification and molecular mechanisms of pain, role of amino acids and known peptides in pain perception mechanisms 36 Chapter Two A Simple Quantitative HPLC method for measuring Physiologic amino acids...

218
264
0

Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

Ngày tải lên : 06/03/2014, 11:20

... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino ... of the fulllength proteins Recombinant SUR2A and SUR2B hydrolyzed MgATP slowly, with maximal turnover 2656 rates of 6.1 · 10)3 s)1 and 2.3 · 10)3 s)1, and Km values of 373 and 38 lm, respectively ... [8] This may also reﬂect construct differences: our NBD1 is 31 amino acids longer at the N-terminus, and our NBD2A is 26 amino acids shorter at the N-terminus, than those of Park et al [8] To...

9
620
0

Báo cáo khoa học: Amino acids Thr56 and Thr58 are not essential for elongation factor 2 function in yeast potx

Ngày tải lên : 07/03/2014, 05:20

... base without amino acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids ... speciﬁc amino acids in bacterial EFG [26–29] To date, there are only a few reports in which this technique has been used to acquire information on the importance of speciﬁc amino acids and amino ... ammonium sulphate) The medium was supplemented with uracil (20 lgÆmL)1) and the appropriate amino acids: histidine (20 lgÆmL)1) and leucine (60 lgÆmL)1) as indicated Galactose (2% weight by volume)...

13
424
0

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

Ngày tải lên : 07/03/2014, 15:20

... tail (amino acids 1–18), the helical transmembrane region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and ... Vibrio aminopeptidase and the alignment of partial amino acid sequences from human GCPII, human transferrin receptor, yeast aminopeptidase Y, S griseus aminopeptidase, and Caenorhabditis elegans ... clearly show that the amino acids at the extreme C-terminus of GCPII are crucial for the hydrolytic activity of the enzyme and, furthermore, that no more than 60 amino acids can be deleted from...

9
414
0

Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx

Ngày tải lên : 17/03/2014, 09:20

... several amino acids may be essential for hepatocellular taurocholate uptake because mutations in these amino acids caused lack of transport; the amino acids in question are Asp115, Glu257, and Cys266 ... Turku, Finland) Results Search for conserved negatively charged amino acids, cysteines and C-terminal threonines by sequence identity An alignment of the amino acid sequence of nine SBAT proteins, ... blocks of amino acids Mutated amino acids are highlighted in gray The resulting mutants are shown in boxes, with deletions indicated (del) Fig Mutations of negatively charged conserved amino acids...

11
367
0

Báo cáo Y học: Amino acids 3–13 and amino acids in and ﬂanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

Ngày tải lên : 17/03/2014, 10:20

... of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and 25 increased the interaction capacity All alanine substitutions of amino acids ... positions 15 and 37, the putative a-helix is ﬂanked by proline residues Within the helix, two candidate FxxFF protein interaction motifs (F is any hydrophobic amino acid residue and x is any amino acid ... The ligand-dependent interaction between AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and in Ó FEBS 2002 Interaction between androgen...

12
597
0

Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

Ngày tải lên : 22/03/2014, 21:21

... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids ... negatively charged amino acids, and instead preferred aliphatic or noncharged amino acids at this position The identiﬁcation of Arg143 and Lys192 as P2¢ preference-determining amino acids facilitates...

13
424
0

Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc

Ngày tải lên : 23/03/2014, 10:21

... Expression and puriﬁcation of wild-type and mutant enzymes Full-length and truncated CpcE and CpcF proteins were expressed with N-terminal His- and S-tags using the pET-30a vector The proteins ... full-length cpcF, P5 and P2 for cpcE(42–276), P1 and P6 for cpcE(1–272), P1 and P7 for cpcE(1–274), P1 and P8 for cpcE(1–237), P9 and P4 for cpcF(21–213), P10 and P4 for cpcF(10–213), P3 and P11 for cpcF(1–160), ... showed more homology: a 10 amino acid truncation reduced the activity to 26%, and it was lost completely when 20 amino acids were truncated Among the 10 N-terminal amino acids, I9 is highly conserved...

13
436
0

Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt

Ngày tải lên : 30/03/2014, 01:20

... The high levels of glutamine and glutamate (nitrogen-rich ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc ... ()61TTATTT)56 and )37TTATTT)32), GLU2 [)506TTATTT)501 and )90TTATTT)85 ()strand)], GLT ()311TATAAAT)305), carA ()277TATATAA)271 and )188TTATTT)183), and carB [)361TTATTT)356 and )143TTATTT)138 ()strand)] ... the biosynthesis and transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological...

16
384
0

Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx

Ngày tải lên : 11/08/2014, 23:21

... effect of branched-chain amino acids and arginine on glucose disposal and performance was also investigated Nine well-trained male wrestlers participated in trials in a random order Each trial ... The peak and average power in the matches was similar in the trials After the supplementation, CHO and CHO+AA trial showed significantly higher glucose and insulin, and lower glycerol and non-esterified ... [38] Since whey protein hydrolyate containes approximately 13.4% amino acids as BCAA and arginine [17], we selected 0.1 g amino acids/ kg/hr in this study A limitation of this study is that muscle...

46
298
0

Báo cáo y học: " Albumin dialysis improves hepatic encephalopathy and decreases circulating phenolic aromatic amino acids in patients with alcoholic hepatitis and severe liver failure" doc

Ngày tải lên : 13/08/2014, 11:23

... tests, arterial ammonia levels, and the concentration of total amino acids, branched amino acids, phenolic aromatic amino acids and tryptophan by high-performance liquid chromatography Briefly, ... total, branched and aromatic amino acids Results are shown in patients with alcoholic hepatitis (AH) and pruritus (control) Circulating levels of total, branched and aromatic amino acids before ... • Total amino acid and phenolic aromatic amino acids diminished and the Fischer ratio increased in patients with alcoholic hepatitis treated with MARS • Changes in amino acid levels and the Fischer...

8
338
0

Iron(III) and copper(II) complexes bearing 8 quinolinol with amino acids mixed ligands synthesis, characterization and antibacterial investigation

Ngày tải lên : 02/09/2015, 13:46

... primary ligand can exhibit biological activity (Noorulla and Sreenivasulu, 2011; Singh et al., 2010; Freeman, 1973; Che and Siu, 2010; Podunavac-Kuzmanovic and Cvetkovic, 2007) and an amino acid ... Iron(III) and copper(II) complexes bearing 8-quinolinol with amino- acids mixed ligands 745 0.5 χmT / cm3Kmol -1 0.4 0.3 0.2 0.1 0.0 20 40 60 80 100 T/K Figure IR spectra of the ligands and complexes ... Hydroxyquinoline (HQ) and amino acids (AA) mixed ligands with Fe(III) and Cu(II) ions producing four bioactive metal complexes have been synthesized The results of spectroscopy, elemental analyses and molar...

6
473
0

Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions

Ngày tải lên : 05/10/2015, 13:53

... domains and important amino acid residues for MRP4 function 45 1.7.1 Substrate binding domains .45 1.7.2 Identification of the key amino acids .47 1.8 Resistance to purine and ... growth and viability To gain insight into the role of key amino acid residues of MRP4 protein for transport organic anions and resistance to chemotherapeutic compounds, three aromatic amino acids ... 20 proteins (Table 1.1) known in both eukaryotic and prokaryotic organisms ABC proteins transport a wide spectrum of substrate including ions, phospholipids, steroids, polysaccharides, amino acids...

139
544
0

Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids

Ngày tải lên : 25/10/2013, 08:20

... C.C., and Won, C.Y (1999) Amino acid-based bioanalogous polymers Synthesis, and study of regular poly(ester amides) based on bis(α -amino acid) α,ω-alkylene diesters, and aliphatic dicarboxylic acids ... Gomurashvili, Z., Katsarava, R., and Kricheldorf, H.R (2000) Amino acid based bioanalogous polymers Synthesis and study of new poly(ester amide)s composed of hydrophobic α -amino acids and dianhydrohexitoles ... and Katsarava, R (1994) Heterochain polymers based on natural amino acids Synthesis and enzymatic hydrolysis of regular poly(ester amide)s based on bis(L-phenylalanine)α,ω-alkylene diesters and...

26
348
0

Microbial Production of Amino Acids in Japan

Ngày tải lên : 26/10/2013, 02:20

... other amino acids Today a whole array of amino acids are produced by microbial methods and used in the fields of medicine and food technology, and in the chemical industry Estimated output and ... Production of Amino Acids in Japan Table Amino acid production in Japan and the world in 1996 Amino acid Method Japan Glycine l-Alanine dl-Alanine l-Aspertate l-Aspergine l-Arginine and analogs ... succinylase, succinyl diaminopimalate desuccinylase, diaminopimelate dehydrogenase, and diaminopimelate decarboxylase The host-vector system of Corynebacterium was already established and the introduction...

15
758
5

Tài liệu Proteins và Amino Acids trong thức ăn thủy sản pptx

Ngày tải lên : 26/01/2014, 18:20

... lượng) ng) Enzymes (các proteins hồng cầu ảnh hưởng điều ng nh ng hòa biến dưỡng) Plasma proteins (các proteins di động thể ng miễn dòch thể ) Hormones (vai trò điều hoà) Proteins thành phần nh ... ester hoá Protein trích ly: peptides, protein biến đổi tính chất nhiệt hay acid hóa ng Proteins liên kết Nucleoproteins: protein + nucleic acid (e.g., seed germs) glycoproteins: protein + COH group ... động cân protein sinh tổng hợp tiêu hao protein cho lượng chức sinh học khác Oxy hóa amino acids Các amino acids bò khữ gốc NH3 phản ứng (oxy hóa) hay chuyển gốc amin Khữ gốc NH3 phương thức chuyển...

36
850
2

Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc

Ngày tải lên : 19/02/2014, 16:20

... L-Met and TPL being 95.23 mM and 2.64 unitsÆmL)1, respectively One-milliliter aliquots were withdrawn after 108, 250, 360, 568, and 754 and treated as above The theoretical values of isotopic ... process of isotopic exchange in amino acids may be described by the kinetic Scheme The attainable degree of the exchange is determined by the isotopic purity of D2O, which is high, and the equilibrium ... transformation Values of KSH and kf(H) correspond to KD and kf for the reaction of nondeuterated substrate, and KSD, kf(D) and kr(D) are equal, respectively, to KD, kf and kr for the reaction of...

7
532
0

Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx

Ngày tải lên : 07/03/2014, 00:20

... involved in the action of amino acids on gene expression Amino acid(s) manipulations Factor(s) implied Inhibiting effect resulting from the presence of amino acids Pooled amino acids deprivation Increased ... the effects of amino acids In addition to ATF/C/EBP factors and speciﬁc AARE sequences, a few other transcription factors and their corresponding cis elements are modulated by amino acids (Table ... C/EBPb, C/EBPc, FoxO3A and Gadd45 mRNA levels Stimulating effect resulting from the presence of amino acids Dietary protein restriction Inhibited HNF-4 and NF1 binding Mixed amino acids addition Increased...

19
361
0