some useful mathematical tables

... particularly useful in dening individualized therapy which may prove to be critical in the treatment of cancer Other Clinical Applications Stem cell-derived hepatocytes would also be useful as in ... dispase digestion, most 12.5-day fetal liver cells are dissociated into single cells (inset ), but some remain in small clusters (arrows) (b) After immunomagnetic separation in the presence of Ca2+, ... clinical applicability Liver stem cells provide a potentially limitless source of cells that would be useful for a variety of clinical applications These stem cells or hepatocytes generated from them...

Ngày tải lên: 29/05/2014, 17:06

... and neural stem cells [12] These observations indicate that mild hypoxia may be a useful tool for expansion of some stem cells for clinical use in vitro at low cost However, the molecular mechanisms ... hypoxia-induced stabilization and activation of HIF1a, which is degraded by the ubiquitin–proteasome system under normoxic conditions [21] It has been reported that a hypoxic environment is essential ... NPCs T Zhao et al ability of hypoxia to induce proliferation These suggest that mild hypoxia is a useful measure for expansion of embryonic NPCs in vitro and that HIF-1a is the causative molecule...

Ngày tải lên: 30/03/2014, 04:20

... migrational ability to VEGF and SDF-1 has been a more refined EPC definition [34] However there is still some controversy as to the precise phenotype of the EPC, since the term implies only ability to ... proteinaceous pulmonary edema fluid and lack of oxygen uptake ability [58] In this condition there has been some speculation that circulating EPC may be capable of restoring injured lung endothelium For example, ... infarct remodeling [78] One caveat of these studies is that definition of EPC was variable, or in some cases a confounding effect of coadministered cells with regenerative potential may be present...

Ngày tải lên: 18/06/2014, 15:20

... cells for autotransplantation Lancet 1989, 2(8663):580-5 Leone AM, Galiuto L, Garramone B, et al: Usefulness of granulocyte colony-stimulating factor in patients with a large anterior wall acute...

Ngày tải lên: 18/06/2014, 16:20

... first months CNIs, such as ciclosporin A or tacrolimus, are the mainstay of immunosuppression, sometimes in combination with mycophenolic acid (MPA) Further treatment options are also available, ... and achieve a state of long-term transplant acceptance completely without immunosuppression for some recipients To apply MAPCs in the clinic, we think that the calcineurin inhibitor-free “bottom-up”...

Ngày tải lên: 18/06/2014, 22:20

... first months CNIs, such as ciclosporin A or tacrolimus, are the mainstay of immunosuppression, sometimes in combination with mycophenolic acid (MPA) Further treatment options are also available, ... and achieve a state of long-term transplant acceptance completely without immunosuppression for some recipients To apply MAPCs in the clinic, we think that the calcineurin inhibitor-free “bottom-up”...

Ngày tải lên: 20/06/2014, 04:20

... first event per patient was counted The number of events may exceed the cumulative total because some events may have occurred in the same patient MI denotes myocardial infarction † This category ... atherosclerosis and initiate a cascade of events that culminates in acute myocardial infarction Some triggering phenomena may exert a single, transient effect on the pathophysiologic process, ... myocardial infarction: initial formation of plaque in a focal area of the artery; progression of some plaques to become vulnerable to disruption; precipitation of plaque disruption through either...

Ngày tải lên: 27/06/2014, 00:20

... widely recognized neurological syndromes Despite the clear evidence of the neurotoxicity of at least some forms of chemotherapy, studies on the effects of chemotherapeutic compounds on brain cells ... contrast with effects on Olig2+/BrdU+ populations in the CC, our analyses raise the possibility of a somewhat enhanced loss of DCX+ cells from among the BrdU+ population in both the SVZ and DG (Figure ... long-lasting depletion of populations required for cell replenishment Nonetheless, the fact that some BrdU-incorporating cells remained in all brain regions examined raises the question of whether...

Ngày tải lên: 06/08/2014, 18:21

... culture expanded osteoarthritic cartilage cells To study the possible multilineage capacity of some OC derived cells, we differentiated these cell cultures toward the osteogenic, adipogenic and ... adipogenic lineage as early as weeks after induction (Fig 5g,5h) Using PCR, the expression of peroxisome proliferator-activated receptor-γ demonstrated adipogenic differentiation by progenitor marker ... the markers CD9, CD44, CD54, CD90 and CD166 The amount of total triple positive cells varied to some extent between the selected combinations, indicating that the subpopulations – although exhibiting...

Ngày tải lên: 09/08/2014, 01:23

Ngày tải lên: 09/08/2014, 07:20

Ngày tải lên: 09/08/2014, 10:20

... sequences and are listed in Table 1: (a) adipogenesis genes: lipoprotein lipase (LPL) and peroxisome proliferator-activated receptor-gamma (PPARγ), (b) osteogenesis genes: alkaline phosphatase ... stimulated with IFN-γ (100 ng/mL) for 72 hours in DMEM supplemented with L-tryptophan (100 μg/mL) In some experiments, a neutralizing antibody (anti-IFN-γR1, 1.5 μg/mL; R&D Systems, Inc.) was added ... glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; OC, osteocalcin; PPARγ, peroxisome proliferator-activated receptor-gamma Page of 12 (page number not for citation purposes) Available...

Ngày tải lên: 09/08/2014, 10:23

... Stuhlmann H, Koup RA, Landau NR: Homozygous defect in HIV-1 coreceptor accounts for resistance of some multiply exposed individuals to HIV-1 infection Cell 1996, 86:367-377 Goila R, Banerjea AC:...

Ngày tải lên: 10/08/2014, 05:20

... diluted in blocking buffer and incubated for hrs at room temperature in the dark condition.), In some cases, nucleus was co-stained with DAPI (4’-6-diamidino-2-phenylindole) staining solution (1:100,...

Ngày tải lên: 10/08/2014, 05:21

... gene expressions, we performed qRT-PCR assays We detected mRNA levels of Sall4 isoforms as well as some important HSC/HPC regulatory genes at day post lentiviral transduction Cells were collected ... that more than 95% of the separated cells were Lin-/Sca-1+ cells by flow cytometric analysis In some cases, fluorescence-activated cell sorting of Lin-/Sca-1+/c-Kit+ (LSK) was performed on the...

Ngày tải lên: 10/08/2014, 21:23

... rafts in human epidermal keratinocytes and internalised into early endosomes then transferred to late endosomes or lysosomes [14] For our Figure (a) Geometric structure of the CdSe QD with one...

Ngày tải lên: 11/08/2014, 00:22

... from pulmonary inflammation and fibrosis at day (not shown; [12]) At this time point, we found some EGFP+ cells positive for SP-C (Figure 3A), but nearly all were negative for the myofibroblast-specific ... 30-40%; Additional file 1, Figure S3A) Analysis of fibrotic lungs (d21) demonstrated that around some EGFP + cells became aSMA+ myofibroblasts, but not SP-C + type II epithelial or btubulin IV-expressing ... transplanted into C57Bl/6 mice by intratracheal injection hours or days after bleomycin challenge Some of engrafted EGFP+ cells injected hours after bleomycin treatment were SP-C-positive (A), but...

Ngày tải lên: 12/08/2014, 14:20

... in blood the mature endothelial cells may originate from sloughing off the vessel wall following some form of vascular insult, higher numbers of circulating EPC seem consistently associated with ... persistent fibrotic changes if circulating EPC numbers remained low during pneumonia, therefore suggest some role in the evolution or repair of such tissular injury [56] In healthy adults, the concentration ... specimens The formalin-fixed tissue was paraffin-embedded and cut at µm to 10 µm (as given in detail in some experiments) Slides were heated in an incubator at 70°C for 30 before they were deparaffinized...

Ngày tải lên: 12/08/2014, 15:20

Ngày tải lên: 12/08/2014, 15:21

... muscle, fat, pancreas, skin, and nervous system, and, interestingly, in cartilage [1-5] Although some of the above non-cartilage MPCs are accessible more easily and in higher numbers, MPCs resident ... of the cells and an overestimation of the percentage of MPCs in native OA cartilage [4] This is somewhat unlikely since the percentage of CD166 + cells decreased from approximately 87% immediately ... mediator in cell adhesion, growth, and migration [27] Indeed, CD166+ cells from OA patients were somewhat more potent in osteogenesis and were the only cells capable of undergoing chondrogenesis...

Ngày tải lên: 12/08/2014, 15:23