somatic mutations of the apc gene in other human cancers

... reamplifying the corresponding fragment and repeating the sequencing procedure using both forward and reverse primers in the samples fixed in Bouin's solution Regarding the codon 81, 75% of the samples ... (12 of 17) of the formalin-fixed samples, and 38% (10 of 26) of the Bouin's fixed tissues Thus, the VHL gene was entirely sequenced (ie 100% of the coding sequence analysed) for a total of 26 ... http://www.occup-med.com/content/2/1/13 Figure Sequence chromatograms of the mutations identified in the VHL gene in this study Sequence chromatograms of the mutations identified in the VHL gene in this study R and T are DNA from...

Ngày tải lên: 20/06/2014, 00:20

... assess the possible functional effect of a sequence variation in the coding regions of the ATM gene resulting in an amino-acid substitution in the protein sequence The online tool scores the effect ... illustration of the ATM gene The distribution of the variations detected in the studied materials along the gene is shown in the upper panel with exonic variants indicated on top of the gene and intronic ... patterns of the ATM gene have revealed low recombination and extensive LD spanning the whole gene, in particular in the 3'- end of the gene, with few haplotypes representing the majority of chromosomes...

Ngày tải lên: 09/08/2014, 10:21

... deletion is in the third exon of the gene and is a frameshift mutation resulting in the production of an inactive, truncated protein Other mutations associated with increased muscling have also ... However, these two groups were very similar, supporting the view that the calf’s myostatin genotype is more in uential than that of its dam in determining the difficulty of calving The objective of ... significance using a likelihood ratio test comprising the log-likelihoods from analyses including and excluding the component from the model Other factors considered in the model were interactions of farm...

Ngày tải lên: 09/08/2014, 18:21

... of manganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study Rather, the involvement of manganese in the ... R2F-protein of the C ammoniagenes ribonucleotide reductase were transformants from the reintroduction of the nrdF gene into the strain of its origin description [4] after the development of an efficient ... that of other class RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E We consider the first option unlikely Under these circumstances, the...

Ngày tải lên: 15/02/2014, 01:20

... 6) for the remaining introns Finally, the 3¢ UTRs of the guinea pig CYP11B genes show only 42% similarity whereas the 3¢UTR of the guinea pig CYP11B2 gene shares up to 72% identity with the corresponding ... position 366 while all other proteins of the CYP11B family including CYP11B2 of the guinea pig have a basic residue (arginine or lysine, respectively) at the corresponding position These two basic residues ... function of the hormonal system We first cloned the genes of the CYP11B family of the guinea pig The 11bhydroxylase of the guinea pig showed higher substrate specificity than the aldosterone synthase In...

Ngày tải lên: 22/02/2014, 07:20

... box of desA (nucleotides 7–12 of the iron box), explaining the regulation of desA expression by binding of DmdR1 to the iron box The same overlapping was found in S pilosus DmdR1 binds to the ... for the treatment of iron overload during metabolic alterations in humans Initial work on the biosynthesis of desferrioxamine B in S pilosus indicated that the first step in the desferrioxamine ... Regulation of the desferrioxamine gene cluster B Fig Binding of the DmdR1 protein to the desA promoter region of either S pilosus (left) or S coelicolor (right) at increasing protein concentrations The...

Ngày tải lên: 16/03/2014, 11:20

... promoter promoter integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated integrated in in in in in in in in in in in in attB attB ... downstream of pyrG were used to generate PCR products covering the entire pyrG gene downstream of synthetic promoters Expression from pyrG in the wild type is regulated by the concentration of CTP in the ... of the CTP synthase decient strain CJ295 (pyrG::ISS1), which also carries a mutation in the cdd gene encoding cytidine deaminase (Fig 1) in order to prevent degradation of cytidine added to the...

Ngày tải lên: 16/03/2014, 16:20

... homologue of the H subunit from the reaction centre of photosynthetic bacteria This is in line with the effect of the protein on the herbicide binding in PSII [34] and also in line with the data in ... on the stromal side of the PsbH protein are involved in the bicarbonate binding From this point of view it is interesting that our results indicated destabilization of the bicarbonate binding in ... as indicated by inhibition of the D1± D2 adduct formation in the IC7 strain The PsbH protein affects the bicarbonate binding on the acceptor side of PSII Fig Identi®cation of the PsbH protein...

Ngày tải lên: 17/03/2014, 11:20

... activity in HepG2 cells The relative positions of the MREs within the promoter region of the WD gene are indicated in Fig The four MREs are located in the proximal region of the WD gene promoter ... presence of MREs in the promoter of the WD gene suggests that MREs and their cognate binding proteins function in the regulation of WD gene expression Because the T1 and C3 nucleotides within the ... arrowheads Fig Microsequencing analysis of the MREa-binding proteins (A) The N-terminal sequence of the 82-kDa band was identical to amino-acid positions 6–16 of the Ku-80 protein (B) The 70-kDa band did...

Ngày tải lên: 17/03/2014, 23:20

... treatment of A549 cells with increasing concentrations of the Sp1 inhibitor mithramycin A (Fig 3B), which not only interferes with the transcription of genes containing GC-rich regions in their promoters, ... difference in the C-terminal domain of TAp73c is that, owing to the excision of exon 11 during alternative splicing, it lacks most of the Glu ⁄ Pro-rich domain and the Pro-rich domain, which are ... alternative splicing in the 3¢-end (leading to the formation of a, b, c, d, e, f and g isoforms) [8–12]; (b) the use of an extrinsic promoter (P1) and an alternative, intrinsic promoter (P2) in the 5¢-end...

Ngày tải lên: 29/03/2014, 09:20

... region of the SERPINE1 gene [32,33] The protein products of the SERPINE1 gene play important roles in TGFβ-mediated biological processes such as fibrosis and wound healing [34] The induction of SERPINE1 ... of the molecules involved in angiogenesis, such as VEGF, FLT1, NRP1, NRP2, ANGPT2, JAG1, ARTS1, TNFRSF12A Also the gene expression of a group of insulin-like growth factor binding proteins, including ... involving the SMAD, a family of proteins similar to the http://www.translational-medicine.com/content/7/1/12 gene products of the Drosophila gene "mothers against decapentaplegic" (Mad) and the...

Ngày tải lên: 18/06/2014, 15:20

... variability was further highlighted by the finding that, in many of the positions, the variant amino acid in the SRV-2 isolate was an amino acid found in either or both of the more distantly related SRV1 ... polymerase during the PCR amplification step These data revealed no evidence for the presence of multiple strains of SRV-2 within a single individual We further analyzed the genetic variation within an ... polymerase errors Genetic variation of SRV-2 within an individual infected macaque In order to determine the genetic variation of SRV-2 within the same animal, multiple clones of a PCR amplification...

Ngày tải lên: 19/06/2014, 08:20

... variability was further highlighted by the finding that, in many of the positions, the variant amino acid in the SRV-2 isolate was an amino acid found in either or both of the more distantly related SRV1 ... polymerase during the PCR amplification step These data revealed no evidence for the presence of multiple strains of SRV-2 within a single individual We further analyzed the genetic variation within an ... polymerase errors Genetic variation of SRV-2 within an individual infected macaque In order to determine the genetic variation of SRV-2 within the same animal, multiple clones of a PCR amplification...

Ngày tải lên: 20/06/2014, 04:20

... prevalence of canine intestinal parasites in 1997, 2002 and 2007 Parasites Giardia intestinalis Group 1a b Others Isospora spp Group 1a b Others Toxocara canis Group 2a Othersb Ancylostoma caninum ... presented to individual veterinary clinics regardless of illness history and living in the Hachinohe area of Tohoku region in Aomori prefecture of Japan, in 1997, 2002 and 2007, respectively Hachinohe ... of intestinal parasites in private household dogs had decreased compared to 10 years ago, since the knowledge of owners regarding canine intestinal parasites and parasitic zoonoses has been increased...

Ngày tải lên: 07/08/2014, 23:22

... concentration decreased the percentage of somatic embryos that expressed secondary embryogenesis None of these studies investigated the histological origin and structural organisation of the somatic embryos ... embryo at the beginning of cotyledonary Secondary somatic embryogenesis in Quercus robur stage, which was excised from one acorn collected in July 1989 in the region of Heillecourt (Lorraine, NE ... 3.2.1 Indirect secondary embryogenesis The initial evidence of indirect secondary embryogenesis consisted in cell divisions occurring within the cortical tissue (figure 2-1) that provoked the swelling...

Ngày tải lên: 08/08/2014, 14:20

... to the log of the optic density The sum of the logarithms of the pixel values was used to estimate the amount of nucleic acid in a band The fold increase was compared based on the expression of ... remove the ubiquitin moiety from a target protein The deubiquitinating enzymes are also involved in processing of poly-ubiquitin precursors into ubiquitin monomers, targeting or salvage of proteasomal ... expressed bands into pCR-TRAP vectors The clones were screened according to the size of the inserts, using PCR and the primer pair that flanked the cloning site of the pCR-TRAP vector and then sequenced...

Ngày tải lên: 08/08/2014, 17:20

... analysis of p53 mRNA and protein in this family in order to further elucidate the consequences of the mutation in the expression of p53 and the possible mechanism of carcinogenesis in carriers of the ... 133 (in exon 5), which at the DNA level is not affected in the case reported on here, making possible the existence of the Δ133p53 isoforms, which could participate in the carcinogenesis of the ... negative, inactivating the p53 protein generated by the wildtype allele and blocking activities such as induction of apoptosis This could explain the pathogenicity of the insertion/duplication in this...

Ngày tải lên: 09/08/2014, 04:21

... scattering of the data in the non-24CAs group was similar to that in the 24CAs group This indicated that the functional behavior of the samples included in the non24CAs group, in spite of the inclusion ... CD154 gene As with other cytokine genes, the 3' UTR of the CD154 mRNA contains binding sites for RNA–protein complexes that are responsible for the lability of the mRNA It has been found that the ... replications of a single individual to estimate the variation inherent in the assay, but it is likely that a significant amount of the scattering in the data is produced by interindividual variations in...

Ngày tải lên: 09/08/2014, 10:21

... (BCL2L1) for The relative amounts of the genes of interest and the b2M reference gene were measured in two independent assays The 2(-ΔΔCT) method was used to present the data of the genes of interest ... between the BCL11B gene and the other four genes of interest in the healthy controls Discussion Increasing numbers of translocations involving the BCL11B locus [8,10,11] or high levels of BCL11B ... electrophoresis analysis The size of the PCR products of the b2M gene used for the BCL11B reference is 332 bp (line 1, 2) and that used for the four genes of interest is 145 bp (line 4-11) Line 3: DNA ladder...

Ngày tải lên: 10/08/2014, 22:21

... strains However, little information is available regarding other biological characteristics of the Meq-null virus In the present study, we examined the oncogenic potential of GX0101ΔMeq The results ... Figure Incidence of mortality in chickens inoculated with bac-GX0101 and GX0101ΔMeq Chickens were inoculated with 1000 PFU of the indicated viruses when they were one-day-old and maintained in isolation ... GX0101ΔMeq exhibited the same replication rate in CEF as bac-GX0101 To determine whether the deletion of the Meq gene has any effect on replication of GX0101ΔMeq in vitro, the growth rate of GX0101ΔMeq...

Ngày tải lên: 11/08/2014, 21:21