solid lipid nanoparticles interaction with cells cytokine production and enzymatic degradation

... pattern is very common with SLNs and was reported by many researchers [38, 39] Recently, Rahman et al [40] studied compositional variations and the interaction of the solid lipid nanoparticles formulation ... vitro and cell uptake studies for targeting of ligand anchored nanoparticles for colon tumors Eur J Pharm Sci 2008; 35: 404-416 39 Liu J, Gong T, Wang C, et al Solid lipid nanoparticles loaded with ... BDH Laboratories (Poole, England) All other reagents and chemicals were of analytical grade Preparation of solid lipid nanoparticles Weighed amounts of soyalecithin and Dynasan were dissolved in...

Ngày tải lên: 25/10/2012, 11:22

... phenotype, e.g., cells rounded up and became semidetached from the Petri dish Both loose and semidetached cytotoxic cells were washed free from bacteria and transferred to a new Petri dish and incubated ... anti-ExoS antibodies (D) and (E) Cells were lysed and samples were separated by SDS ⁄ PAGE Western blot analysis was performed on immunoblotted filters with anti-Ras (D) and with anti-Erk (E) antibodies ... analysis of HeLa cells infected with variants of ExoS HeLa cells in the presence of 0.1% arabinose, were infected with: (B) and (F) YPIII (pMF384) expressing arabinose induced ExoS(wt); (C) and (G) YPIII(pMF516)...

Ngày tải lên: 19/02/2014, 08:20

... tRNA and tmRNA alanylation under standard reaction conditions and 50 °C should be close to 0.8 s)1 and 0.03 s)1, respectively This is to be compared with kcat values of 0.93 s)1 [27], 1.1 s)1 and ... mobility shift study of the interaction between EF-Tu*GDPNP and uncharged tmRNA Two equivalent gels were run at identical conditions and stained with Coomassie Blue R250 (A) and with pyronin Y enhanced ... the interaction with similar structural patterns (the alanylated acceptor stem with the specifically recognizable GU base pair, and the T loop) On the other hand, in an established complex with...

Ngày tải lên: 08/03/2014, 08:20

... in the interaction with RNA and DNA in the elongation complex At elevated ionic strength, Rif and r70 suppressed RNAP–aptamer interactions (above) Under low ionic strength conditions, Rif and r70 ... reports on interactions of the core with total cellular DNA and RNA [43,46], tRNA [47,48], ssDNA [43] and also some individual RNA sequences [49] Reported Kd values for some of these interactions ... the aptamers (Fig 5B and data not shown) Specific and nonspecific interactions of aptamers with the RNAP main channel The interaction of the aptamers with RNAP was found to be highly dependent on...

Ngày tải lên: 23/03/2014, 13:20

... associated with increased inflammation and Th1 cytokine production [4] These results suggest that Tec kinases contribute to human diseases involving distinct types of T-cell activation and cytokine production ... interactions with other hematopoietic cells [24,28] Selection through interactions with other hematopoietic cells is not a phenomena unique to CD8+ innate T cells Invariant natural killer T cells (iNKT) ... Th1 and Th2 cells, in which high antigen dose has been shown to lead to IFN-c production and low antigen or altered peptide ligands that induce partial TCR signaling preferentially induce IL-4 production...

Ngày tải lên: 28/03/2014, 22:21

... with or without relapse and patients with or without NAbs At each time point, a Mann-Whitney test was performed to identify differences in biological markers and clinical measure between HD and ... [D-erythro-6-(1’,2’,3’-trihydroxypropyl)-pterin] production [6] and trp degradation [7,8] In vitro evidence demonstrated that both IFNb and IFNg induce neopterin production [9] and activate the enzyme indoleamine ... time and drug dose; B: kyn/trp ratio as function of time and drug dose Neopterin production (A) and tryptophan degradation (as measured by kynurenine/tryptophan ratio) (B) in patients treated with...

Ngày tải lên: 18/06/2014, 19:20

... with or without relapse and patients with or without NAbs At each time point, a Mann-Whitney test was performed to identify differences in biological markers and clinical measure between HD and ... [D-erythro-6-(1’,2’,3’-trihydroxypropyl)-pterin] production [6] and trp degradation [7,8] In vitro evidence demonstrated that both IFNb and IFNg induce neopterin production [9] and activate the enzyme indoleamine ... time and drug dose; B: kyn/trp ratio as function of time and drug dose Neopterin production (A) and tryptophan degradation (as measured by kynurenine/tryptophan ratio) (B) in patients treated with...

Ngày tải lên: 20/06/2014, 03:20

... 0.05) Without LPS stimulation, increasing concentrations of glucose (250, 500, and 1,000 mg/dL) did not cause an increase in IL-1β and IL-6 cytokine release compared with samples stimulated with ... increase in cytokine production (Figure 1d) Combination of glucose and insulin on cytokine release To determine whether the hyperglycemic effect on cytokine release seen in Figures 1a and 1c could ... substances (glucose and mannitol) on phagocytic and oxidative burst activity in human granulocytes hour, after which sample and standard probes were added to each well (50 μL/well) and incubated overnight...

Ngày tải lên: 13/08/2014, 11:22

... Critical Care Vol 12 No Wade cytokine response and the absence of a consistent attenuation of the response with insulin suggest that factors other than hyperosmolality and hypertonicity are involved ... in function correlates with a decrease in inflammation and tissue damage in vivo In contrast, Otto and colleagues demonstrated an increase in activity of PBMC function with exposure to hyperglycemia, ... enhances cytokine production and decreases phagocytosis in vitro Crit Care 2008, 12:R107 Shukla A, Hashiguchi N, Chen Y, Coimbra R, Hoyt DB, Junger WG: Osmotic regulation of cell function and possible...

Ngày tải lên: 13/08/2014, 11:22

... rheumatoid arthritis (RA) and from healthy controls (HC) CD4+ T cells (5 × 105 cells in 0.5 ml culture medium with 10% FCS) were incubated with or without IL-10 (1 and 10 ng/ml) and cells were harvested ... antigens HLA-DRB1*0404 and HLA-DRB1*0401 with disease susceptibility and severity [16,17], and by the high-level expression of MHC class II molecules and both CD28 ligands, CD80 and CD86, in the inflamed ... T cells to IL-10, purified PB CD4+ T cells from three patients with active RA and from three healthy controls were stimulated by immobilized anti-CD3 antibody and anti-CD28 antibody with or without...

Ngày tải lên: 09/08/2014, 01:24

... Akt, 5-LOX ⁄ FLAP and NADPH oxidase inhibitors on SEC1 induced pyrogenic cytokine production and fever index in rabbits Human PBMC (1 · 107 cells mL)1) were pretreated with or without PDTC (1000 ... the febrile response, two classes of cytokines have been reported: endogenous pyrogenic cytokines (IL-1 and IL-6) and endogenous antipyretic cytokines (IL-10 and tumor necrosis factor-a) [50] In ... of IL-1b and IL-6 was 1.3 · 108 UÆmg)1 and · 106 UÆmL)1, respectively Preparations of whole cell lysates and nuclear fractions )1 Human PBMC (1 · 10 cells mL ) were treated with or without the...

Ngày tải lên: 19/02/2014, 00:20

... the cholesterol docking site within the START domains of STARD1 and STARD3, we performed photoaffinity labeling with [3H]photocholesterol and subsequent chemical or enzymatic cleavage of the photoactivated ... STARD1START is well suited for a ligand with the size and shape of cholesterol [29,30] Critical residues proposed to interact with cholesterol are localized within the fragment containing amino ... yellow, and comprises half of the a3-helix and the strands b3–b7, including their connecting loops The residues Glu107 (E), Arg126 (R) and Leu137 (L) (all marked in magenta) are located within...

Ngày tải lên: 30/03/2014, 04:20

... Lymphoid cells (1 × 106 cells/ well) were plated onto a monolayer of RA-SNC77 cells and incubated for hours The lymphoid cells bound to the surface of the RA-SNC77 cells were removed, and the interaction ... surface of RA-SNC77 cells were removed, and the interaction between these cells was examined with a phase-contrast microscope RA-SNC77 cells with more than three lymphoma cells beneath them were ... Cellular interaction between human lymphoid cells and RA-SNC77 cells (a) Adhesion between lymphoid cell lines and RA-SNC77 Biscarboxyethyl carboxyfluorescein-labeled lymphoid cells (2 × 105 cells/ well)...

Ngày tải lên: 09/08/2014, 06:22

... tissue-culture plates for TEM analysis and phagocytosis experiments Media was changed on day Page of and day 5, and after days of culture the MDMs were used for phagocytosis and cytokine assays Platelets ... by microscopy) and cells were incubated 15 at 37°C Macrophages were then collected and fixed in 1% cold paraformaldehyde and analyzed using a CyAn flow cytometer (Beckman Coulter) and the Summit ... 14,000 g, and frozen at -80°C Cytokines were measured by ELISA using capture and detection antibodies (eBioscience) per the manufacturer’s instructions Cytokines were measured in duplicate and averaged...

Ngày tải lên: 11/08/2014, 03:20

... evaluated before and after incubation with two cytokines involved in the activation process of these cells, namely IFN-g and TNF-a Results from IFN-g and TNF-a are shown in Figures and 2, respectively ... and patients, before and after activation with different concentrations of tumoral necrosis factor-alpha (TNF-a), and after challenge with C albicans The results are expressed as mean ± SEM and ... endothelium and synthesis of other pro-inflammatory cytokines such as IL-1 and IL-6 Similar to results regarding pro-inflammatory cytokines, high levels of anti-inflammatory cytokines (IL-10 and TGF-b)...

Ngày tải lên: 11/08/2014, 03:20

... stimulation with inflammatory chemokines and cytokines and/ or combined with TLR agonists present at the site, DCs migrate to the lymph nodes and activate the adaptive immune response, and subsequently ... TIMP1 and DC cocktail screening and validation Potent IL-12p70 and TNFa stimulating cocktails were designed by series of combinations of pro-inflammatory cytokines and TLR agonists The cytokines ... 5000 mononuclear cells were gated using a combination of forward-angle and side scatter to exclude dead cells and debris Data were analysed with FACSDiva software Western blot Cox2, and actin expression...

Ngày tải lên: 11/08/2014, 06:22

... tissue-culture plates for TEM analysis and phagocytosis experiments Media was changed on day Page of and day 5, and after days of culture the MDMs were used for phagocytosis and cytokine assays Platelets ... by microscopy) and cells were incubated 15 at 37°C Macrophages were then collected and fixed in 1% cold paraformaldehyde and analyzed using a CyAn flow cytometer (Beckman Coulter) and the Summit ... 14,000 g, and frozen at -80°C Cytokines were measured by ELISA using capture and detection antibodies (eBioscience) per the manufacturer’s instructions Cytokines were measured in duplicate and averaged...

Ngày tải lên: 11/08/2014, 06:22

... and (a1) treated with CTLA4-Ig 500 μg/ml for IL-6, (b) untreated (controls) and (b1) treated with TNFalpha, (c) untreated (controls) and (c1) treated with IL-1β and (d) untreated (controls) and ... ELISA for IL-6 and IL-1β confirmed a slight, dose-dependent reduction in cytokine production in the media of co-cultures after treatment with CTLA-4-Ig as compared with untreated cells (Figure ... downregulate SM activation (co-cultured with T cells) by interacting with the B7.2 molecule expressed on their surface and by decreasing their production of inflammatory RA cytokines (IL-6, TNFα, IL-1β)...

Ngày tải lên: 12/08/2014, 11:22

... treated with: A IL-1β (10 ng/ml) with and without BAI (30 μM), and B TNF-α (100 U/ml) with and without BAI (30 μM) for hours before harvested for RNA preparation RNA was subjected to RT-PCR with ... TNF-α-induced IL-6, IL-8, and MCP-1 production in mast cells First, the effect of BAI on production of the inflammatory cytokines, IL-6, IL-8, and MCP-1, from IL-1β- and TNF-αactivated HMC-1 cells was studied ... IL-8, and MCP-1 in IL-1β- and TNF-α-activated HMC-1 cells RT-PCR RT-PCR analysis of effects of BAI on the gene expression of IL-6, IL-8, and MCP-1 in IL-1β- and TNF-α-activated HMC-1 cells HMC-1 cells...

Ngày tải lên: 13/08/2014, 13:22

... viability of B3Z T cells, Jurkat T cells and DC2.4 cells after infection with B pseudomallei 0.2 x 106 B3Z T cells (A), Jurkat T cells (B) and DC2.4 cells (C) were infected with B pseudomallei ... VITRO INTERACTION BETWEEN BURKHOLDERIA PSEUDOMALLEI AND HOST T CELLS AND DENDRITIC CELLS 22 INTRODUCTION Bacterial internalization and their ability to survive and replicate within host cells ... cell viability of B3Z T cells and Jurkat T cells with DC2.4 cells When B3Z T cells were infected at MOI 5:1 and 30:1, cell viability of infected cells remained above 90 % and 85 % respectively...

Ngày tải lên: 08/11/2015, 16:31