... mortality by statins, angiotensin-converting enzyme inhibitors, and angiotensin receptor blockers in patients with chronic obstructive pulmonary disease J Am Coll Cardiol 20 06, 47 :25 54 -25 60 Page of ... Med 20 03, 167: 127 1- 127 8 32 McKay A, Leung BP, McInnes IB, Thomson NC, Liew FY: A novel antiinflammatory role of simvastatin in a murine model of allergic asthma J Immunol 20 04, 1 72: 2903 -29 08 ... I and pharmacokinetic study of farnesyl protein transferase inhibitor R115777 in advanced cancer J Clin Oncol 20 00, 18: 927 -941 22 Standards for the diagnosis and care of patients with chronic...
... during tissue fibrosis J Clin Invest 20 02, 110(3):341-350 Page 13 of 15 (page number not for citation purposes) Respiratory Research 20 09, 10:100 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 ... Zuraw BL: The anti-inflammatory effect of glucocorticoids is mediated by glucocorticoid-induced leucine zipper in epithelial cells J Allergy Clin Immunol 20 07, 119(1):115- 122 Eddleston J, Christiansen ... bronchial biopsies [21 ,22 ] The increase in TGFβ1 was shown to persist despite oral corticosteroid treatment [22 ,23 ] and to correlate with basement membrane thickness and fibroblast number [24 ] We hypothesized...
... regulation in the growth plate and CCN FEBS Journal 27 4 (20 07) 1655–1665 ª 20 07 The Authors Journal compilation ª 20 07 FEBS T Yanagita et al 19 20 21 22 23 24 25 family member expression in cartilage ... were culturedin a-modification of minimum essential medium (Sigma, St Louis, MO) containing 10% fetal bovine serum Cell cultures were maintained in a humidified 5% CO2 atmosphere at 37 °C without ... Cell culture A human chondrosarcoma-derived chondrocytic cell line, HCS -2 ⁄ [ 32] , human cervical carcinoma-derived HeLa, human melanoma cell line A371, human osteoblastic SaOs2 and human embryonic...
... secretion by corticosteroids in bleomycin-induced pulmonary inflammation in the rat J Clin Invest 1993, 92: 18 12- 1818 Chen Y, Chen J, Dong J, Liu W: Antifibrotic effect of Interferon gamma in silicosis ... [14] Determination of hydroxyproline content The lung samples were measured for hydroxyproline content using a hydroxyproline kit from Nanjing Jian Cheng Institute (China) following instructions ... standard curve contained concentrations ranging from 31 .25 to 20 00 pg/ml of porcine TGF-β1 (R&D Systems, Minneapolis, USA) The plates were incubated at 37°C in 5% CO2 for 24 h, then added 10...
... Blood glucose (mg/dl) 0.5h 1h 127 .63± 12. 68 104.75 20 .55 Diabetic control group 300.63±104.69a 24 4.13±107.03b 22 2.75±104.81 b 20 1.38±91.64 b 20 9.50±87.61 a 20 3.75±100.30 a 180 .25 ±111. 82 b Diabetic ... GLP-1-(7-36)NH2, GLP-1-(7-37), and GLP-1- (9-36)NH2 on intravenous glucose tolerance and glucose-induced insulin secretion in healthy humans J Clin Endocrinol Metab 20 03; 4: 17 72- 9 Green BD, ... dissolved in sodium citrate buffer) after overnight fasting [10] Mice in normal control group were treated with sodium citrate buffer 72h after injection, the mice with fasting blood glucose higher...
... used in the PCR to amplify the human ZC3H12A second intron fragment, ranging from +27 91 to +29 82, and containing postulated NF-jB-binding sites As a positive control a PCR was carried out with ... as well as within the gene [8–10] In order to localize potential regulatory elements in the human ZC3H12A locus containing NF-jB-binding sites, computational transcription factor binding-site prediction ... enhancer contains four NF-jB-binding sites To increase the sensitivity of NF-jB-binding site prediction within the EnhA sequence, computational analysis was repeated using the consite online tool...
... both to learning and to recovery from neural injury [1], perhaps by enhancing synaptogenesis by influencing Reelin signaling [2, 3] In humans, single-nucleotide polymorphisms in the coding region ... shown that the increase in ApoE brain levels that occurs with aging continues to occur in AD, with a large fraction being deposited in plaques [35] This increase in ApoE levels is distinguishable ... 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Griffin WS, Yeralan O, Sheng JG, Boop FA, Mrak RE, Rovnaghi CR, Burnett BA, Feoktistova A, Van Eldik LJ: Overexpression of the neurotrophic cytokine...
... Cheshire JL, Williams BRG, Baldwin AS Jr: Involvement of 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 double-stranded RNA-activated protein kinase in the synergistic activation of ... is indicated by ‘?’ 2- AP, 2- aminopurine; MMP, matrix metalloproteinase; TNF, tumour necrosis factor C2-ceramide in the presence of the PKR inhibitor 2- AP With the addition of 10 mM 2- AP, the C2-ceramideinduced ... sGAG into the medium increased 1.5-fold after treatment with C2-ceramide at 10 µM (P = 0.0 92) and 50 µM (P = 0.05) and two-fold with C2-ceramide at 100 µM (P = 0. 029 ) (Fig 3b) 2- aminopurine inhibits...
... diffusional constraints in unstrained constructs in the bioreactor, involving contact of the upper surface with a fluid impermeable loading pin Accordingly, the mass transport of oxygen into the construct ... expression in articular chondrocytes Matrix Biol 20 02, 21 :25 1 -26 2 23 Malemud CJ: Protein kinases in chondrocyte signaling and osteoarthritis Clin Orthop Relat Res 20 04, 427 (suppl):145-151 24 Badger ... regulation of iNOS and COX -2 by dynamic compression, co- stimulation with the inhibitor was important in restoring the cytokine-induced inhibition of aggrecan expression This is in contrast to collagen...
... stained sections were counterstained with Mayer's hematoxylin for 10 seconds and mounted with aqueous mounting medium For human STEAP4 staining, synovial tissues were obtained after informed consent ... anti -human CD68 (BD Pharmingen, San Diego, CA, USA) conjugated with rhodamine (1:100 dilution, Rhodamine protein labeling kit; Pierce) were used Nuclei were counterstained with 4'-6'diamidine -2- phenylindole ... of TIARP in joints was upregulated at day 28 , but not at day 14, in mice with collagen-induced arthritis and that expression correlated with joint swelling (data not shown) These findings suggest...
... washed in PBS, incubated in biotinylated secondary antibody for 60 min, washed again in PBS, and then incubated in an avidin-rich solution for 60 After a final wash, positive biotin-avidin binding ... box (atrogin-1); MuRF-1: muscle ring finger protein-1; MHC: myosin heavy chain; TGFβ1: Transforming Growth Factor-β1 Competing interests The authors declare that they have no competing interests ... HIV-related pathology and immunologic dysfunction Proc Natl Acad Sci USA 20 01, 98: 927 1- 927 6 22 23 24 25 26 27 28 29 30 31 32 Reid W, Abdelwahab S, Sadowska M, Huso D, Neal A, Ahearn A, Bryant J,...
... categories in plants Trends Plant Sci 20 05, 10:117- 122 22 23 24 25 26 27 Giuliani C, Consonni G, Gavazzi G, Colombo M, Dolfini S: Programmed cell death during embryogenesis in maize Ann Bot 20 02, 90 :28 7 -29 2 ... 7-domain-containing proteins (Figure 1) Many of these proteins are lipid raft-associated and may cluster to form membrane micro-domains, and in turn recruit multi-protein complexes functioning in ... protein kinase 4) during plant defense response to incompatible pathogen Plant Pathol J 20 07, 23 :76-89 Zhou J, Loh Y, Bressan R, Martin G: The tomato gene Pti1 encodes a serine/threonine kinase...
... TGF-β1-induced MMP -2 activity by BMP-7 is in accordance with the inhibition shown for MMP-13 BMP-7 could contribute to a reduction in airway remodelling by inhibiting some MMPs without affecting ... harvested using RIPA buffer (Invitrogen) following the manufacturer's instructions Protein concentration was determined using the BCA protein assay (Pierce), against a bovine serum albumin standard ... 30 in a buffer containing 2. 7% Triton X-100 at room temperature and incubated for 48 h in 50 mM Tris-base, 40 mM HCl, 20 0 mM NaCl, mM CaCl2, 0. 02% Brij 35, at 37°C The gels were then stained with...
... (D141, E185, D 226 , D244) in PIN domains involved in binding Mg2+ ions are conserved in all three sequences Other conserved residues are R263, V139 and N144 Determination of the MCPIP domain architecture ... transfected with a vector overexpressing MCPIP and with an empty vector (control) using Lipofectamine 20 00, according to the manufacturer’s protocol 4¢-6-diamidino -2- phenylindole (DAPI) staining was ... another protein with confirmed RNase activity – SMG6 (2hww) [7] – has the canonical four conserved acidic residues (D141, E185, D 226 and D244; Fig 2B) that are involved in binding Mg2+ ions during RNA...
... Protective effects of PEP-1–HSP27 against brain ischemia 20 21 22 23 24 25 26 27 28 29 30 31 Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells FEBS Lett 485, ... ischemia in a gerbil model We injected PEP-1–HSP27 fusion protein FEBS Journal 27 5 (20 08) 129 6–1308 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 129 9 Protective effects of PEP-1–HSP27 against ... non-induced PEP-1–HSP27; lane 2, induced PEP-1–HSP27; lane 3, purified PEP-1–HSP27 analysis using antibody against rabbit polyhistidine (Fig 2B) Transduction of PEP-1–HSP27 fusion protein into...