slab gel electrophoresis for protein analysis
Báo cáo khoa học: "Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites" ppt
Ngày tải lên :
07/08/2014, 17:23
... total of 46,5 kVh, (B) In gel rehydration conditions were at V for hrs followed by 30 V for hrs, 200 V for hrs, 500 V for h, 1,000 V for h, 2,000 V for hrs and 4,500 V for 10 hrs, having a total ... tachyzoites SDS-PAGE was performed using 12% gels which were then stained with silver nitrate (A) In gel rehydration were done at V for 12 hrs, 500 V for h, 1,000 V for h and 4,500 V for 10 hrs, having ... 95oC for The lysates were centrifuged at 10,000 g for at 4oC and the supernatants were stored at −70oC The sample was separated by SDSPAGE using 12.5% slab gels with a 4% stacking gel The protein...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1
Ngày tải lên :
14/09/2015, 10:36
... portable capillary electrophoresis system as in the previous chapter, and potentially can be used for field analysis of chiral compounds In addition, a fast method for the analysis of underivertized ... FMOC 9-fluorenylmethyl chloroformate GC Gas chromatography GC-MS Gas chromatography–mass spectrometry HPCE High performance capillary electrophoresis HPLC High performance liquid chromatography ... Human Serum by Capillary Electrophoresis with Contactless Conductivity Detection, Electrophoresis, in press LingLing Yuan, HongPing Wei, HuaTao Feng, Sam Fong Yau Li, Fast Analysis of Native Neomycin...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2
Ngày tải lên :
14/09/2015, 10:37
... traditional slab- gel electrophoresis, both of which separate the analytes according to their charge and size CGE is mostly used for the analysis of large molecules of biological interest such as proteins ... are urgent needs for analytical systems for homeland security Methods for fast analysis of stabilizers in gunpowder and propellants were developed on our portable capillary electrophoresis system ... driving force for the actual migration of species in the column in CE are their charges, the applied potential and EOF 3, Since the capillary applied in CE is normally empty (except Capillary Gel Electrophoresis...
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On line pre concentration techniques in capillary electrophoresis for environmental analysis
Ngày tải lên :
16/09/2015, 15:55
... open-tubular preconcentrator for CE In this approach the wall of a 20-cm capillary was modified with a C18 phase for herbicide analysis, or a metal chelate phase for 16 protein analysis The diluted analytes ... sample self-stacking system for the determination of hippurate in serum.the former provided better performance for real samples [159] This technique can be applied to protein analysis and environmental ... mobilities in a uniform electrophoresis medium This is the most common and the simplest mode in CE CZE can be performed in either free solution or anticonvective media, such as gels A uniform, or homogeneous,...
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Capillary and microchip electrophoresis for the analysis of small biomolecules
Ngày tải lên :
02/10/2015, 12:56
... required for CE analysis Thus, it will be 11 ideal for routine analysis, e.g in clinical analysis, where large numbers of samples need to be examined 1.4 Project objectives The quantitative analysis ... spectrometry CE: Capillary electrophoresis HPLC: High performance liquid CGE: Capillary gel electrophoresis chromatography CIEF: Capillary isoelectric focusing HPLC-MS: High performance liquid COC: ... based IL and the adenosine specific 42-mer was obtained 34 Before any analysis could be performed, the running buffer conditions for the analysis of adenosine via the CE-UV system had to be optimized...
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báo cáo khoa học: " Evaluation of protein pattern changes in roots and leaves of Zea mays plants in response to nitrate availability by two-dimensional gel electrophoresis analysis" pdf
Ngày tải lên :
12/08/2014, 03:21
... This statistical analysis was performed using the software STATISTICA Extraction of protein samples for 2-DE analysis Three independent biological replicates were extracted for each condition ... gels using the ETTAN DALT six apparatus (GE Healthcare) Running was first conducted at W /gel for 30 followed by 15 W /gel until the bromophenol blue line ran off Two replicates were produced for ... change in their relative volumes were considered for successive analyses Protein in -gel digestion and LC-ESI-MS/MS analysis Spots excised from gels stained with cCBB were digested as described...
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Báo cáo khoa học: Analysis of the CK2-dependent phosphorylation of serine 13 in Cdc37 using a phospho-specific antibody and phospho-affinity gel electrophoresis doc
Ngày tải lên :
30/03/2014, 03:20
... required for the efficient recruitment of Hsp90 to protein kinase complexes [17,18] Therefore, Cdc37 activity is crucial for many signaling protein kinases to function correctly in vivo Protein ... filtration tubes (Millipore, Bedford, MA, USA) For phospho-affinity gel electrophoresis analysis, Tris-buffered saline (50 mm Tris ⁄ HCl, 150 mm NaCl, pH 7.4) was used for washing and elution instead ... was incubated at 30 °C for 30 min, analyzed by phospho-affinity gel electrophoresis, and then stained with CBB (B) Wild-type protein (lanes 1, and 7) and two Cdc37 mutant proteins, 13SA (lanes...
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Báo cáo y học: "Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis" pdf
Ngày tải lên :
13/08/2014, 13:20
... 1) 500 V for 500 Vhr, 2) 1000 V for 1000 Vhr, 3) 8000 V for 24 000 Vhr, 4) 8000 V for 64 000 Vhr and 5) 8000 V for 64 000 Vhr For the preparative gel, labeling and rehydration was performed as ... physiological tests [22] Protein quantification was performed using the BCA protein assay kit (with BSA as standard) to create a regression analysis to estimate overall protein concentration for each hourly ... utilize a differential gel electrophoresis (DIGE) approach This involved labeling the pre- and post-shock samples with fluorescent dyes, two-dimensional gel electrophoresis for protein separation,...
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Báo cáo y học: "Two-dimensional difference gel electrophoresis (DIGE) analysis of sera from visceral leishmaniasis patients" ppt
Ngày tải lên :
13/08/2014, 13:20
... performed for every matched spot-set, comparing the average and standard deviation of protein abundance for a given spot Mass spectrometric analysis and protein identification A preparative gel ... current set at 15 mA per gel at 20°C for 30 minutes, followed by 30 mA per gel at 20°C until the bromophenol blue dye front ran off from the bottom of the gels Six such gels were run corresponding ... images were classified as standards The gel with the highest spot count was assigned as the master gel Matching between gels was performed utilizing the in -gel standard from each image pair Matching...
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Báo cáo y học: "Amino acid size, charge, hydropathy indices and matrices for protein structure analysis" docx
Ngày tải lên :
13/08/2014, 23:20
... Identification of amino acids involved in protein structural uniqueness: implication for de novo protein design Protein Eng 2002, 15:555-560 Brunak S, Engelbrecht J: Protein structure and the sequential ... prediction of only one single structure from one primary protein sequence The development of a prediction tool for protein structure (like an mfold for nucleic acids [4]), which provides only a few ... proper environment, a protein would fold up spontaneously into a conformation that minimizes the total free energy of the system None of the protein structure predicting methods perform satisfactorily,...
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Báo cáo y học: "ProCAT: a data analysis approach for protein microarrays" pot
Ngày tải lên :
14/08/2014, 17:22
... cross-reactivities of protein microarrays can not be as directly corrected for A separate slide is, therefore, often required to be probed in parallel as a negative control in protein microarray ... functional protein microarrays in comparison to antibody arrays, and, therefore, the normalization techniques used for DNA microarrays are usually not directly applicable to functional protein microarrays ... changes to the original signals and, therefore, increases the discrepancy between the duplicate spots of the same protein The variance of the signals for the same protein after normalization with different...
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Novel methods for quantitative analysis and evaluation of effects of chronic exposure to microcystins by capillary electrophoresis and metabolomic studies
Ngày tải lên :
11/09/2015, 10:14
... be a gel (as in gel electrophoresis) or can be a liquid, in most cases a buffer solution In capillary electrophoresis, the medium is contained in a small capillary 40 Therefore capillary electrophoresis ... developed for the determination of cyanotoxins in water, for example, the international organisation for standardisation method for determination of microcystins in water (ISO 20179:2005) is high performance ... largely responsible for the phenotype (observable trait) therefore it is responsible for what can happen, however other factors such as the environment can also affect the phenotype Genetic information...
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Development of liquid phase microextraction techniques combined with chromatography and electrophoresis for applications in environmental analysis
Ngày tải lên :
14/09/2015, 11:45
... introduced to a capillary electrophoresis system (CE) for analysis The LODs, which ranged from 0.001 to 0.003 µg/ml, were low enough for the determination of phenols in environmental analysis In addition, ... MICROEXTRACTION TECHNIQUES COMBINED WITH CHROMATOGRAPHY AND ELECTROPHORESIS FOR APPLICATIONS IN ENVIRONMENTAL ANALYSIS by ZHANG JIE (M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT ... (for GC applications) Therefore, since its introduction, SPME has been widely applied in many fields including environmental, food, natural products, biological, forensic and pharmaceutical analysis...
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GEL PROTEIN, CẤU TRÚC VÀ PHƯƠNG PHÁP TẠO CẤU TRÚC GEL CỦA CÁC PROTEIN TRONG CÁC THỰC PHẨM GIÀU PROTEIN
Ngày tải lên :
24/04/2013, 10:09
... hợp tạo cho gel có tính bất thuận nghịch nhiệt, bền Một số protein có chất khác tạo gel đun nóng đồng thời (sự đồng tạo gel) Các protein tạo gel cách cho tương tác với chất đồng tạo gel polysacarit, ... nồng độ protein thay đổi 4.Tính chất tạo gel số potein thực phẩm Protein vân Khả tạo gel nhiệt protein miofibril (tơ cơ) thịt cá sở kết cấu nhiều sản phẩm thực phẩm Do tạo mạng lưới gel nên protein ... cao thường cản trở tạo gel nồng độ thấp nồng độ cao (khoảng 30%) tạo gel Tinh bột đồng tạo gel với protein Nhờ tương tác mà khả giữ nước, độ cứng độ đàn hồi gel protein tốt Gel từ tinh bột giàu...
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Tài liệu Báo cáo khoa học: A systems biology approach for the analysis of carbohydrate dynamics during acclimation to low temperature in Arabidopsis thaliana doc
Ngày tải lên :
14/02/2014, 22:20
... infrared gas analysis Measurements were performed during the first h 508 of the light phase, representing the time period of photosynthetic activity until plants were harvested for analysis of ... search method for bound constrained gloabal optimization J Glob Optim 39, 197–219 34 Schmidt H & Jirstrand M (2006) Systems Biology Toolbox for MATLAB: a computational platform for research in ... rates of carbon uptake before and during the first day of cold acclimation After days of cold exposure, the mean rate of carbon uptake was significantly lower for C24 than for Rsch (P = 0.03), and...
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Tài liệu the supply oF money – banK behaViour and the implications For monetary analysis doc
Ngày tải lên :
16/02/2014, 11:20
... compensation for holding these instruments in the form of interest rate remuneration and monetary services The monetary services compensate the depositors for the interest they forego by holding ... banks has considerable implications for the macroeconomy The examination of money and credit developments for the purposes of monetary analysis provides information on the functioning of banks’ ... (see Box entitled “Multiplier analysis of the effect of monetary policy on money supply”) The supply of money – bank behaviour and the implications for monetary analysis See Brunner, K and Meltzer,...
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Tài liệu Báo cáo khoa học: Cell-free translation systems for protein engineering docx
Ngày tải lên :
19/02/2014, 06:20
... a strong tool for producing proteins Production of biologically active proteins In order for the cell-free translation system to produce biologically active proteins, additional proteins such ... strategies for efficient synthesis of biologically active proteins Correct disulfide bond formation in proteins such as antibodies can be facilitated by the addition of the redox-dependent chaperone protein ... ribosome, to form a link consisting of protein ⁄ tRNA ⁄ ribosome ⁄ mRNA In the in vitro virus or mRNA display, a covalently linked Cell-free translation for protein engineering mRNA ⁄ puromycin ⁄ protein...
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Tài liệu Báo cáo khoa học: "A study of Information Retrieval weighting schemes for sentiment analysis" doc
Ngày tải lên :
20/02/2014, 04:20
... et al., 1996) is a probabilistic model for information retrieval and is one of the most popular and effective algorithms used in information retrieval For ease of reference, we incorporate the ... as the former are scaled and the latter smoothed in some manner For example, a∆(p′ )n vs a∆(t′ )n perform quite similarly The results from the Multi-Domain Sentiment data set (figure 2) largely ... comparison of event models for naive bayes text classification 1394 G Mishne 2005 Experiments with mood classification in blog posts In 1st Workshop on Stylistic Analysis Of Text For Information Access Fabrizio...
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