size exclusion chromatography in structural analysis of intrinsically disordered proteins

... BBK proteins in the vertebrates; the MATH-BTB proteins in the worm; the BTB-NPH3 proteins in the plant; the Skp1 proteins in the plant and worm; and the T1 proteins in worm and vertebrates In ... bZip-containing proteins (550 of 738 Pfam bZip_1 domain) contain no other identified domains in interactions A large expansion of MATH-BTB proteins occurred in C elegans, where 46 of 178 total BTB proteins ... domain from RPT2 can interact with a region of phototropin that contains light, oxygen and voltage sensing (LOV) protein-protein interaction domains [103] These proteins consist of an amino-terminal...

Ngày tải lên: 14/08/2014, 14:22

... Comparing and combining predictors of mostly disordered proteins Biochemistry 2005, 44:1989-2000 Uversky VN, Oldfield CJ, Dunker AK: Intrinsically disordered proteins in human diseases: introducing ... contained genuine high levels of disorder in proteins A third scheme to classify disorder in proteins was based on the percentage of disordered residues in the protein The percentage of disordered ... Comparing the disordered proteins with the ordered proteins, we observe increases of 33.57% and 12.8% in the percentage of proteins possessing at least one ubiquitination site and the percentage of...

Ngày tải lên: 14/08/2014, 21:20

... occurrence of single intraresidue NOE between the respective H-1 and H-2 resonances The structure of the Hex4 glycoform in strain RM6255 was determined by examining the 1H-NMR spectrum of its LPS-OH in ... detected in H in uenzae LPS by a method involving analysis by HPAEC-PAD of terminal N-acetyl neuraminic acid residues released by neuraminidase treatment [7] However, by applying this method in our ... experienced a rapid decline in diseases caused by Hib strains In the UK, Hib infection decreased 98% in children younger than years of age [22], thus resulting in a greater proportion of infections due...

Ngày tải lên: 23/03/2014, 21:20

... cavity of the lectin protomer) A closer examination of the structure of 3728 the binding sites indicates that specificity of the Moraceae lectins is primarily determined by the orientation of the ... plays a key role in the delineation of the size and shape of the binding site The presence in loop of bulky amino acid residues has apparently no effect on the accessibility of monosaccharides ... residues of the monosaccharide-binding site of MornigaM E F Fig Structure and surface analysis of MornigaM and jacalin (A, B) Ribbon diagrams showing the arrangement of the 12 b-strands of the...

Ngày tải lên: 30/03/2014, 20:20

... OS-1b,c a Minor amounts of sialylated Hex3 glycoforms were indicated b Minor amounts of Hex2 and Hex3 glycoforms lacking acetyl groups or containing a second glycyl group were indicated c Very minor ... H-5, H-7 of Kdoa H-3 of HepI; H-1 of HepIII H-1, H-2 of HepII; H-1 of GlcII; H-1 of GlcII* H-4, H-6 of HepI H-4, H-6 of HepI H-4, H-6 of HepI H-1, H-2 of HepIII H-1, H-2 of HepIII H-4 of GlcII*d,e ... 25 Methylation analysis of dephosphorylated OS-1 showed significantly increasing amounts of terminal Glc and 2-substituted Hep, thereby indicating phosphorylation of these residues in the native...

Ngày tải lên: 31/03/2014, 21:21

... by modifying the values of w and q For example, using a window size of w = 53 (instead of 41) and a downsampling factor of q = 13 (instead of 10) simulates a tempo change of the original data ... a link of minimal cost, referred to as initial link, and construct a path in a greedy fashion by iteratively adding links of low cost, referred to as admissible links In step (2), all links in ... sequence of length N = 901, corresponding to 15 minutes of audio Tripling the length N by using a threefold concatenation of the Bolero results in a running time of 37.9 seconds, showing an increase...

Ngày tải lên: 22/06/2014, 23:20

... backbone to increase stability of the interaction Cooperative binding of ssb proteins assists in unwinding the DNA duplex at replication forks, recombination intermediates, and origins of replication ... gp32 single stranded binding protein increases the processivity of the RNase H The two proteins interact between the C-terminal domain of RNase H and the core domain of gp32 on the 3’ arm of the ... clamp proteins participate in many protein/DNA interactions including DNA replication, repair, and repair signaling proteins A multitude of different proteins have been identified that contain a...

Ngày tải lên: 11/08/2014, 21:21

... Khangildin VV: Investigation of new mutant genes in the pea Diallele analysis of seed productivity of the nonshattering pea cultivars containing def gene Genetika 1989, 25:659-665 Khangildin WV, ... growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the drafting of ... and examination For structural analysis, seeds of all lines were embedded in LR White resin (London Resin Company, England) Seeds from each pod identification stage were transversely cut into mm...

Ngày tải lên: 12/08/2014, 03:20

... 1.6 Roles of actin binding proteins in actin dynamics (A) Actin monomer binding proteins Thymosin-β4 blocks all assembly reactions; profilin promotes nucleotide exchange and inhibits pointed-end ... Gelsolin/villin superfamily severs actin filaments Twinfilin inhibits actin nucleotide exchange Actin/Bundling/Crosslinking Proteins α-actinin connects and organizes actin filaments EPLIN stabilizes ... myosins in budding yeast (D) Cytokinesis in animal cells by constriction of a contractile ring of actin filaments and myosin II (E) Cytokinesis in fission yeast The contractile ring of actin filaments...

Ngày tải lên: 11/09/2015, 10:17

... ultrapure water, drinking water, rain water, river water and seawater under various pH and irradiation sources (sunlight, darkness, indoor incandescent lighting and artificial UV lighting) Hydrolysis ... pH-dependent irrespective of the type of water matrix Light intensity could prompt degradation significantly under mild alkaline conditions, while its influence was minor at higher pH (pH > 9) ... conjugate of 2-isopropoxyphenol occurred in all tested biological samples under the applied MAE conditions and led to low recoveries The stability of propoxur under microwave irradiation was maintained...

Ngày tải lên: 16/09/2015, 17:13

... follows: 20-ml of methanol, an optimum microwave heating temperature of 80 °C, and a 6-min of microwave heating Table 5-4 Variance analysis table in the OA (27) matrix for the optimization of MAE from ... within 1-2 minutes of microwave heating during the extraction An important point to note when employing the OAD procedure is that although some parameters (duration of extraction and volume of ... power setting for the microwave heating This setting was proportional to the number of samples undergoing extraction during one run Furthermore, according to previous experience with MAE and intuition,...

Ngày tải lên: 16/09/2015, 17:13

... increased to 200°C at a rate of 25°C/min, then maintained at 200°C for min; a second ramp 230°C at rate of 5°C/min, increased to 280°C at a rate of 20°C/min; The final temperature of 280°C was held for ... methanol 88 An interesting result observed from Fig 4-6 is that in the same solvent, the recovery increases in going from PAHs to PCBs, and then to triazines (i.e, increasing polarity of analyte) ... the recovery of PAHs, PCBs and triazines For example, the recoveries of triazines progressively improved with an increasing degree of moisture in the tested soil This can be explained by the high...

Ngày tải lên: 16/09/2015, 17:13

... degradation rates increase in the order of increasing pH (seawater [pH 7.8] or river water [pH 7.6] > drinking water [pH 7.03] > rain water [pH 4.2]) For example, the percentage of degradation in seawater ... application of LC-APCI-MS for the determination of carbamates: carbofuran and 3hydroxy-carbofuran, and the analysis of triazine herbicides in water [8] LC-ESI-MS has been ... enhance degradation of propoxur in sea and river water, Seiber et al reported a remarkable decrease in the halflife of Carbofuran in paddy field water instead of distilled water [16] In addition, microbial...

Ngày tải lên: 16/09/2015, 17:13

... Polychlorinated biphenyls (PCB-1242 and PCB-1248) were purchased as individual standard stock solutions containing a nominal concentration of 100 µg/ml in methanol from Ultra-Scientific Atrazine (purity ... a Finnigan MAT LCQ ion-trap mass spectrometer (all ThermoQuest, San Jose, CA, USA) The instrument was initially tuned based on a mixture of caffeine, L-methionyl-arginyl-phenylananyl-alanine ... lactuca) were purchased in a local market In order to remove possible trace organic contaminants prior to preparation, all of them were washed in fresh running water and then rinsed with deionized...

Ngày tải lên: 16/09/2015, 17:13

... molecular-mass determination for large proteins by means of ESI At present, APCI is commercially available and a growing interest in applications involving biological and environmental analysis In an APCI ... Table 1-3 Comparison of the boiling points of solvents in open and closed systems [64] Solvent Dichloromethane Boiling point (°C) in open system 39.8* Boiling point (°C) in closed system (175psi) ... breakthrough in the development of coupling techniques was the introduction of the moving-belt interface (MB) in 1974 that, became the first commercial LC-MS interface [11] Subsequently, many interface...

Ngày tải lên: 16/09/2015, 17:13

... Triostin A and echinomycin are represented in sticks Triostin A is coloured in pink and echinomycin in cyan; (b) Superimposition of the crystal structures of triostin A and echinomycin Minor deviations ... is on the structural study of the enzyme Ecm18 involved in the final step of the biosynthetic pathway of echinomycin 1.3.1 Biosynthesis of echinomycin The biosynthesis of echinomycin follows ... Rossmann-like fold / SAM-binding domain 40 3.3.1 Conserved motifs involved in SAM/SAH binding 40 3.4 Substrate binding domain 41 iii 3.5 Conformation of echinomycin in Ecm18 ...

Ngày tải lên: 04/10/2015, 10:23

... Triostin A and echinomycin are represented in sticks Triostin A is coloured in pink and echinomycin in cyan; (b) Superimposition of the crystal structures of triostin A and echinomycin Minor deviations ... is on the structural study of the enzyme Ecm18 involved in the final step of the biosynthetic pathway of echinomycin 1.3.1 Biosynthesis of echinomycin The biosynthesis of echinomycin follows ... Rossmann-like fold / SAM-binding domain 40 3.3.1 Conserved motifs involved in SAM/SAH binding 40 3.4 Substrate binding domain 41 iii 3.5 Conformation of echinomycin in Ecm18 ...

Ngày tải lên: 12/10/2015, 17:36

... errors in the learning process of English are inevitable The errors in using prepositions, especially the prepositions of place AT, ON, IN are not exceptional The teaching and learning of prepositions ... in language learning Secondly, to identify causes of learner's errors Finally, to obtain information on common difficulties in language learning, as an aid to teaching or in the preparation of ... source of L1 interference in producing L2 (Dulay et al, 1982) The first source is a lack of common knowledge and linguistic competence at a certain stage of their learning This often occurs in early...

Ngày tải lên: 20/12/2013, 18:17

Ngày tải lên: 17/02/2014, 20:20

... International Journal of Finance, The Journal of Banking and Finance, The Journal of Finance, The Journal of Financial Economics, The Journal of Financial & Quantitative Analysis, The Journal of Industrial ... ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE AND ACCOUNTING Essays in Microstructure in Honor of David K Whitcomb Volume This page intentionally left blank ADVANCES IN QUANTITATIVE ANALYSIS OF FINANCE ... contribution of microstructure can be seen in the advancement of our understanding of market efficiency In particular, we can now use intraday data to examine the speed of information incorporation into...

Ngày tải lên: 19/02/2014, 13:20