site1 the high affinity site

... motifs in the other, thus offering not only the highest total number of reactive motifs among the p53DBSs tested, but also the highest number of sites known to be modified most frequently (i.e the GG ... between the sensitivity of the p53 sequence-specific DNA binding to DNA treatment with cisplatin and the ability of the particular p53 target site to accommodate the cisplatin IACs The higher the ... the cisplatin IACs within the p53DBSs due to the occurrence of the GG, AG and ⁄ or GNG motifs, the stronger the inhibition of the p53 sequence-specific DNA binding to these targets caused by the...

Ngày tải lên: 19/02/2014, 05:20

... abolished the internalization of the GFP-fused Tat protein but did not alter the uptake of the Tat CPP These biochemical evidences con®rmed a pathway for the entry of the Tat peptide unrelated to the ... routes for the Tat CPP and the Tat protein What might be the reasons underlying the observed differences in cellular uptake between the Tat CPP and the GST±Tat±GFP protein, that both contain the same ... amounts of the rhodamine-labeled Tat peptide, as indicated in the ®gure Comparative FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPP Differences in the mechanisms...

Ngày tải lên: 21/02/2014, 03:20

... (together with Pex30 to Pex32) might affect peroxisome number These models are nonexclusive with each other, and the mechanism of action will not be the same for all PPPs In the light of the different ... [42], namely (a) the insertion of Pex11 into the membrane, (b) the elongation of peroxisomes, (c) the segregation of Pex11 and the formation of Pex11enriched patches, and (d) the division of peroxisomes ... complex from the matrix to the inner membrane of the peroxisome The membrane-bound pool of acylCoA oxidase interacts with Pex16, which is also membrane bound inside the peroxisome The substrate–...

Ngày tải lên: 07/03/2014, 21:20

... trap the oligomeric forms that not enter the gel, we used centrifugation assays Following the assay, we recovered a fraction of the protein in the pellet (lane 1, Fig 1B) and the remainder in the ... the protein–DNA complexes that entered into the gel mapped to the position indicated by the asterisk in Fig 1A The ssDNA mediated increase in monomer level remained essentially unchanged in the ... of hRad52 appears to be highly salt sensitive and in the present assay conditions with 20 mm KCl, the protein remains highly aggregated The addition of ssDNA rendered the protein into forms that...

Ngày tải lên: 16/03/2014, 14:20

... and the inferences that can be based on our findings Further studies of the sources of endogenous oxalate synthesis in humans are needed The phenotypic features of Agxt) ⁄ ) mice are probably the ... available for fatty acid biosynthesis Taken together, these results seem to be indicative of an adaptation in favor of liver gluconeogenesis in response to the lack of AGT1 Other downregulated enzymes, ... between the groups (P < 0.01), and two were exclusively detected in knockout mice In 14 of the 18 spots, MALDI peptide mass fingerprints allowed the identification of the corresponding proteins in the...

Ngày tải lên: 23/03/2014, 03:20

... enzymes involved in the synthesis of polar lipids are localized in the endoplasmic reticulum (ER), and the peroxisome is not capable of synthesizing these lipids [65,67] Therefore the lipids have ... dissociation [56] The functions of other components of the import complex are still unknown Whether the RING-finger complex is really involved in peroxisomal matrix protein import or rather in the re-export ... translocated across the peroxisomal membrane while the receptor shuttles back to the cytosol in a so-far unknown manner The so-called extended shuttle hypothesis is based on the assumption that the import...

Ngày tải lên: 23/03/2014, 13:20

... of the multiple cloning sites of the plasmids shown The spacing shows the lacZ reading frame of the translational fusion vectors *** Note that in the case of the Kan ~ plasmids the Sinai site ... a site other than the att site, lysogens need to be constructed by infecting an att deletion strain In the absence of a chromosomal att site, the recombinant phage can only integrate onto the ... recombination events will lead to the transfer of the fusion in the original strain to the plasmid Strains carrying the fusion on the pRS308 vector can then simply be infected with the appropriate )tRS phage...

Ngày tải lên: 10/04/2014, 11:02

... be useful to pass the tagged protein sequence through the PROSITE database or other algorithm that identifies protease cleavage sites to eliminate the possibility that such a site has been inadvertently ... Polypeptide ligands and their cell surface receptors bind to one another with high affinity and specificity These biological properties can be exploited to make affinity probes to detect their cognate ... depends on the individual experiment Most importantly, it depends on the affinity of the ligand-receptor interaction For the interaction of receptor tyrosine kinases with their cognate ligands, the...

Ngày tải lên: 10/04/2014, 11:02

... [107] When MAPK and other factors are released in the circulation in vivo, they bind to the cell surface of endothelial cells and activate them Prolonged activation of endothelial cells by MAPK ... while the newly formed endothelial cells are being differentiated in vivo The VWF modulates these processes and sustains the differentiated state of these cells (Figure 8) In addition, the blood ... CXCR4 and others), the cell surface proteins are clustered This generates a cascade of signals from the plasma membrane to the cytoplasm and nucleus As the new proteins are expressed, the HIV-infected...

Ngày tải lên: 18/06/2014, 15:20

... defined the lower limit for p53-positive tumor cells Another cause of the discrepancy in the reported percentage of p53-positive tumors is the differences in the p53 expression with the prevalent ... the p53 protein expression in the liver tissues adjacent to the tumor in the same group of HCC patients On the basis of our results, the comparison between p53 expression in HCC tissues and the ... N1-7 were the adjacent hepatic tissues The expression of the housekeeping gene, glyceraldehydes 3phosphate dehydrogenase (GAPDH), served as a control The expression of p53 was higher in the HCC...

Ngày tải lên: 18/06/2014, 15:20

... from the viral polyprotein [30,31], and the second is cleavage of the p220 component of the cap-binding protein complex [32], resulting in the shut off of host protein synthesis On the other ... translation initiation site at nt 1079, in addition to the authentic initiation site for the polyprotein at nt 1066 [44] (Fig 2) This alternative initiation site is out-of-frame with the polyprotein ... all the cardioviruses [6,36] Our unpublished data demonstrate that L is synthesized with the same kinetics as capsid proteins and is not incorporated into the virion L is synthesized in the cytoplasm...

Ngày tải lên: 19/06/2014, 22:20

... drafted the manuscript JB performed the flow cytometry RT conceived the study, participated in the design of the study, and with EM performed the statistical analysis All authors read and approved the ... demonstrating yet another novel function of the G protein in the regulation of host cell response In the study reported here, significantly higher levels of IFNβ expression were detected in the cell culture ... affect the spectrum of the antiviral cytokine response [2,15,31,36,51,56], but the mechanisms linked to RSV protein regulation of the associated cell signaling pathway remains unclear The studies...

Ngày tải lên: 20/06/2014, 01:20

... not within the spatial conformation of the native heterocomplex A biological reason for the higher frequency of aRibPR0 might be the disposability of ribosomal P0-like protein in the cell membranes ... Authors’ contributions RB had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis RB, CD, FH and WSchlumberger ... analysis was performed twice, taking either the results of the anti-dsDNA ELISA (Figure 3a) or those of the Farr assay (Figure 3b) Among 163 SLE patients, there were 11 (6.7%) individuals who could...

Ngày tải lên: 12/08/2014, 15:22

... (CMV) promoter upstream of the 3' end of the MMTV genome as well as the Renilla luciferase gene between splice donor and acceptor sites [5] The RmRE appears to span the envelope-3' LTR junction ... (black box) is located between the splice donor (SD) and acceptor (SA) sites The smaller hatched box indicates the SV40 polyadenylation region The smaller gray box shows the position for insertion ... reporter plasmid (in the presence of the control EGFP expression vector only) were assigned values of 1, and the other samples have been reported relative to these values These results are representative...

Ngày tải lên: 13/08/2014, 05:21

... loaded onto the gel Relation4 the intensities of the bands in the lanes with recombinant PFV proteins shown in Fig and amounts of protein Relation of the intensities of the bands in the lanes with ... kD C-terminal peptide from the pr71 Gag precursor (our unpublished results) Therefore, the antibody detects both, the uncleaved and the cleaved protein equally well The 127 kD Pol precursor protein ... p68Gag protein is the dominant capsid protein species in infectious PFV particles The SGG1 MAB binding site is located N-terminal of the Gag cleavage site that generates p68 Gag and the kD C-terminal...

Ngày tải lên: 13/08/2014, 09:21

... Symptomatic of the simplicity of the E cuniculi kinetochore is the absence of the vast majority of potential MAPs Nonetheless, it is significant that the E cuniculi kinetochore contains three of the four ... Cdc20 showing the conserved Mad2 binding site Note: E cuniculi lacks both the conserved Mad2 binding site and an ortholog of the Mad2 protein Schematic drawings indicate the length of the S cerevisiae ... using the highly conserved reference proteins αtubulin, the signal recognition particle subunit SRP54 and PCNA) they were found to cluster closely together (Figure 1f) While recognizing the possibility...

Ngày tải lên: 14/08/2014, 16:21

... parasites that not have their own translational machinery in a host cell Therefore they not separate themselves from the host cells and use the host translational machinery to replicate in the ... when the two domains meet each other in the yeast cells The two proteins for interaction study are separately fused to the above two domains If there is interaction of the two proteins, the transcription ... immobilized affinity ligand specific for the tag, thereby generating a „secondary affinity support‟ for purifying other proteins that interact with the bait protein The secondary affinity support...

Ngày tải lên: 11/09/2015, 10:17

... proteins, the efficiency of decapping is in correlation with the length of the substrate It is hypothesized that the special separation of the RNA binding site and the decapping active site accounts ... (Figure 7b) And the structure of the X29- m7GpppA complex shows that the adenine base of the cap analog is stacked in the active site and the triphosphate group is positioned close to the catalytic ... subunit joins the 40S subunit to form an 80S ribosome The ribosome decodes the mRNA triplet and catalyzes the formation of a peptide bond The growing polypeptide chain exits the ribosome and the elongation...

Ngày tải lên: 14/09/2015, 12:28

... point (hkl) in the reciprocal space The direction of the reciprocal vector corresponds to the plane normal and the magnitude of the reciprocal vector is equal to the reciprocal of the interplanar ... amplitude and the phase While the amplitude is calculated from the intensity of a reflection, the phase of the wave, which depends on the positions of all atoms with respect to the origin of the unit-cell, ... when they are in phase (their amplitudes are magnified as the sum of the two waves) while the resultant wave decreases in amplitude if the waves are out of phase (Fig 1.3) Figure 1.3 1.3.5 The...

Ngày tải lên: 04/10/2015, 10:24