single molecule studies of viral dna packaging
Báo cáo hóa học: " Stretching and immobilization of DNA for studies of protein–DNA interactions at the single-molecule level" ppt
Ngày tải lên :
22/06/2014, 18:20
... different methods of stretching and immobilizing DNA molecules for studying single- molecule DNA protein interactions Single- molecule experiments overcome the problem of ensemble-averaging of molecular ... rate of a single DNA polymerase (DNAP) on a single- stranded DNA (ssDNA) stretched by a magnetic trap as shown in Fig 5A Similarly to RNAP, DNAP reads the Fig (A) Schematic representation of experimental ... destabilizing the DNA (B) Comparison of the elasticity of a charomid and pXD II ssDNA and the WLC model curve for dsDNA The difference in G + C content in ssDNA molecules influences the stability of secondary...
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Single molecule studies on the role of nucleoid associated proteins in bacterial chromatin
Ngày tải lên :
09/09/2015, 11:26
... are capable to probe the DNA binding properties of these important proteins at the singlemolecule level Single- molecule manipulation using magnetic tweezers and single- molecule imaging using atomic ... presence of MvaT and MvaU mixture 67 71 73 75 76 79 81 Single- molecule stretching experiments on Ler -DNA complexes 92 Persistence length and DNA occupancy measurement of LerDNA complexes and H-NS -DNA ... to this thesis Single molecule manipulation method using transverse magnetic tweezers enables us to stretch single DNA molecule and probe the e↵ects of DNA- binding proteins on DNA mechanical...
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Báo cáo sinh học: " Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA" pdf
Ngày tải lên :
19/06/2014, 08:20
... absence of this molecule results in Page of 14 (page number not for citation purposes) Virology Journal 2005, 2:55 delayed onset of viral DNA synthesis as well as inefficient replication of the viral ... onset and expansion of viral DNA synthesis suggest that it functions as part of the DNA replication machinery We propose a model in which pUL114 functions as part of the viral DNA polymerase complex ... subsequent expansion phase of viral DNA synthesis and suggests that this gene product functions as part of the viral DNA replication machinery Discussion Figure in Defects DNA synthesis associated...
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báo cáo hóa học:" Human cytomegalovirus uracil DNA glycosylase associates with ppUL44 and accelerates the accumulation of viral DNA" potx
Ngày tải lên :
20/06/2014, 04:20
... absence of this molecule results in Page of 14 (page number not for citation purposes) Virology Journal 2005, 2:55 delayed onset of viral DNA synthesis as well as inefficient replication of the viral ... onset and expansion of viral DNA synthesis suggest that it functions as part of the DNA replication machinery We propose a model in which pUL114 functions as part of the viral DNA polymerase complex ... subsequent expansion phase of viral DNA synthesis and suggests that this gene product functions as part of the viral DNA replication machinery Discussion Figure in Defects DNA synthesis associated...
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Báo cáo y học: " Single cell studies of the cell cycle and some models" pps
Ngày tải lên :
13/08/2014, 22:22
... scatter, the fine detail of the single cell linear patterns is lost There is also a second RCP at the end of the cycle A much more detailed analysis of populations of single cells followed on ... could profitably be applied to the growth of Escherichia coli The limitations of synchronous cultures in hiding the fine detail of increases in volume or area could well mean that single cell studies ... there was a burst of studies on selected single cells or small groups The growth patterns were often not well defined but it seems that absolute measurements of volume and of respiration rate...
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Báo cáo khoa học: Interaction of the C1 complex of Complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy pdf
Ngày tải lên :
16/03/2014, 23:20
... the site of interaction on the protein For this purpose we took advantage of the binding properties of C1q toward DNA and of an emerging technique allowing the molecular combing of DNA strands ... on the A chain of C1q has been identified as the major binding site of DNA [30] We have analyzed herein the binding of the human of the C1 complex and of its subunit C1q to T4 DNA by molecular ... environment DNA preparation T4 DNA (160 kb) was labeled as follows: 7.2 lL of 1.35 nM T4 DNA were incubated with 10 lM YOYO-1 (Molecular Probes) in ultrapure water This respresents a ratio of molecule of...
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Single DNA studies of architectural proteins involved in bacterial pathogenesis and meiosis in saccharomyces cerevisiae
Ngày tải lên :
09/09/2015, 11:25
... objective of finding the characteristics of protein binding to single DNA molecules needs the aid of singlemolecule manipulation During the last twenty years, single molecule manipulations have brought ... the position of the magnets relative to the bead Such a magnetic tweezers setup allows the probing of the mechanical properties of a single DNA molecule For that, DNA molecules of interest are ... by the DNA bending rigidity DNA conformations are controlled by the interplay between thermal energy and the bending stiffness of DNA backbone In DNA polymer models, the bending energy of DNA with...
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Study of genome packaging using single molecule manipulation and image method
Ngày tải lên :
14/09/2015, 08:39
... micromanipulation of single DNA molecules In brief, the two ends of a DNA molecule (normally the 48.5kb -DNA) is first labeled with biotin- and digoxygenin-labeled oligonucleotides, respectively [2] One end of ... most of the Escherichia coli cells are about µm long and 0.5µm wide, but their chromosomal DNA molecules have a contour length of approximately mm In absence of restriction, such a long DNA molecule ... DNA According to their effects on DNA, these proteins can be roughly divided into classes: DNA wrapper, DNA bender and DNA bridger This classification is based mostly on the topographies of DNA- protein...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
Ngày tải lên :
20/02/2014, 23:20
... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... binding of cAMP to the anti-cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2 ... Structure of the CAPDNA complex at 2.5 A resolution: a complete picture of the protein DNA interface J Mol Biol 260, 395408 Passner, J.M & Steitz, T.A (1997) The structure of a CAPDNA complex...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
Ngày tải lên :
07/03/2014, 16:20
... N-terminal domain of the protein occurs upon binding of DNA to the C-terminal domain of CRP [30] Our uorescence studies of CRPDNA interactions presented here also agree with the results of Baichoo & ... primary-kink site in the CAPDNA complex: DNA binding specicity based on energetics of DNA kinking J Mol Biol 314, 6374 38 Lin S-H & Lee JC (2003) Determinants of DNA bending in the DNA- cyclic AMP receptor ... values of the average Trp13 lifetime instead of the values of lifetimes of the resolved components Binding of cAMP to anticAMP-binding sites leads to signicant changes in the uorescing properties of...
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Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx
Ngày tải lên :
16/03/2014, 13:20
... of IN viral nonviral active viral nonviral C IN IN inactive active Fig Schematic diagram of three models compatible with the formation of the catalytically competent viral INDNA complex (A) DNA ... rate of viral DNA cleavage by IN was investigated under two different conditions: enzyme excess and DNA substrate excess IN displayed only limited turnover for cleavage of viral DNA, and excess viral ... corresponding to viral DNA bound at the nonviral site (normally occupied by the target DNA) , the other corresponding to viral DNA bound at the viral site (specic site) Several studies on mutants...
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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx
Ngày tải lên :
22/03/2014, 18:20
... layer of protein attached to a substrate, the cantilever tip can adsorb a single protein molecule Extension of a molecule by retraction of the piezoelectric positioner results in deflection of the ... the range of 1.7–2.5 .[37, 46] These values of Dxu are comparable to the size of a water molecule, suggesting that water molecules, and thus hydrogen bonds, are integral components of the unfolding ... effects of D2O on the dynamics of protein folding.[36] Knowledge of the influence of D2O on the conformational dynamics of a protein may be important both at a basic level, to identify the nature of...
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Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot
Ngày tải lên :
31/03/2014, 21:21
... (often choline) The nucleases catalyze the hydrolysis of DNA phosphodiester bonds Sequence alignments of PLDs revealed that, with the exception of two nucleases, most PLDs contain two copies of ... length) The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of mM of substrate by 0.125 lM of enzyme in reaction ... with quadruplicate sets of samples Ó FEBS 2002 Kinetic studies of tyrosyl -DNA phosphodiesterase (Eur J Biochem 269) 3703 natural substrate (Topo I DNA complex) that offers a more extensive surface...
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Báo cáo hóa học: " Photoelectrochemical studies of DNA-tagged biomolecules on Au and Au/Ni/Au multilayer nanowires" docx
Ngày tải lên :
20/06/2014, 22:20
... tagging, (iii) Au NWs with tagging of cy3-dye attached DNA, (iv) Au/Ni/Au NWs with tagging of cy3-dye attached DNA PL images of (v) Au NWs and (vi) distinct difference of Au and Ni in Au/Ni/Au multilayer ... underlying concept of low emission intensity is the reduction of Au surface area in Au/Ni/Au multilayers that causes lesser amount of tagging on the NW Consequently, a larger amount of tagged DNA is adsorbed ... http://www.nanoscalereslett.com/content/6/1/535 literature is scarce on the detection of biomolecules using this approach PEC is simple and offers an alternative method of detecting biomolecules through molecular binding on a working...
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báo cáo khoa học: "Influence of viral hepatitis status on prognosis in patients undergoing hepatic resection for hepatocellular carcinoma: a meta-analysis of observational studies" ppt
Ngày tải lên :
09/08/2014, 02:21
... resection Secondary outcomes of interest were clinicopathologic features Results Selection of Studies The search strategy initially generated 38 studies [3-7,10-42] Of these studies, 18 were excluded ... analyzed in many studies Only seven of 20 studies reported 186 cases of such patients in current review [3-5,27-29] To avoid high bias-risk of publication, we did not perform an analysis of BC-HCC group ... data of subsets of patients The characteristics of these 20 studies are summarized in Table Patients Characteristics Results from overall meta-analysis are outlined in Table The mean age of patients...
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báo cáo khoa học: " DNA ligase 1 deficient plants display severe growth defects and delayed repair of both DNA single and double strand breaks" potx
Ngày tải lên :
12/08/2014, 03:20
... activation of DNA repair pathways [3] DNA ligases play essential roles in all organisms by maintaining the physical structure of DNA These enzymes seal gaps in the sugar-phosphate backbone of DNA that ... kinetics of double or single strand DNA breaks respectively [19,20] The Comet assay was used here to investigate the kinetics of DNA repair in atlig1-RNAi lines compared to wild-type plants DNA single ... first report of a role for AtLIG1 in DSB repair and identification of a novel DNA DSB repair pathway in plants Results Phenotypic analyses of DNA ligase1 deficient plants In the absence of viable...
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Báo cáo y học: "Eukaryotic large nucleo-cytoplasmic DNA viruses: Clusters of orthologous genes and reconstruction of viral genome evolution" pps
Ngày tải lên :
12/08/2014, 04:21
... potential of comparative and evolutionary genomics of the NCLDV increase A pre-requisite of an informative comparative-genomic study of any group of organisms is an accurate delineation of the sets of ... Evolution of DNA ligases of Nucleo-Cytoplasmic Large DNA viruses of eukaryotes: a case of hidden complexity Biology Direct 2009, 4(1):51 Banerji S, Aurass P, Flieger A: The manifold phospholipases A of ... reproduction One of the most expansive, apparently monophyletic divisions of viruses consists of at least families of eukaryotic viruses with large DNA genomes including Poxviridae, an expansive viral family...
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Báo cáo khoa học: " Packaging of viral RNAs in virions of adenoviruses" pptx
Ngày tải lên :
12/08/2014, 04:21
... E-I MA RNase DNase + + + + i ii Figure of Analysis viral protein and DNA in HAV5.EGFP capsids Analysis of viral protein and DNA in HAV5.EGFP capsids (A) Proteins from lysates of RNase/DNase treated ... (fiber) of PAdV-3 The viral genomic DNA and RNA were isolated from the same pool of purified mature capsids Viral DNA was used as a template in real-time PCR to determine the total number of viral ... Fiber RNase Figure Quantitation of viral RNAs by real-time PCR Quantitation of viral RNAs by real-time PCR Viral genomic DNA and RNAs were isolated from the same pool of purified mature capsids RNAs...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
Ngày tải lên :
13/08/2014, 09:21
... analysis of total viral DNA synthesis, viral DNA nuclear import and integration indicates that all three C-terminal mutants displayed a class II mutant profile Even though all of them reduced viral ... detect viral gene expression from integrated and unintegrated viral DNA [33] After 48 hours of infection, equal amounts of cells were lysed in 50 µl of luc lysis buffer and then, 10 µl of cell ... reverse transcription and/or viral DNA nuclear import steps, which are different from the action of D64E mutant on viral DNA integration Effect of IN mutants on viral infection in dividing and...
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