single cell differential mrna display

... time-dependent mRNA expression of single living cells, but it is limited to the analysis of the fine localization of mRNA in the cells ISH can examine mRNA expression of the whole cells with higher ... time-lapse change of identical cells because the cells have to be fixed We reported a single cell nanoprobe (SCN) method to examine mRNA expression without killing cells in a previous report [12-14] ... Comparison between single cell nanoprobe method and ISH result (± S.D) ISH intensity Single cell nanoprobe method 0–0.1 β-actin mRNA detection probability Average number of detected β-actin mRNA Hybridization...

Ngày tải lên: 11/08/2014, 00:22

... Single cell protein concept  Single cell protein is a terms for a type of cell nutrient and it produced by microorganisms  Microbial species capable of single cell protein synthesis ... disadvantages Advantages of single cell protein: • Disadvantages of • Protein in high cell • Slow digestion by the cell structure • • Good quality protein • Safety toxins single cell protein: • High ... single cell protein: • High nucleic acids produce many uric acids in the blood Application of single cell protein In the food industry: • E.g: produce bread, beer, sausage … In the pharmaceutical:...

Ngày tải lên: 03/01/2013, 12:52

... the cells is necessary for the maintenance of oscillations Removal of external Ca2+ leads to a cessation of oscillations in most cases in endodermal cells [62] and HeLa cells [63] In other cell ... However, cellular Ca2+ transients also have a spatial dimension In the cytoplasm of single cells, Ca2+ gradients can be observed when Ca2+ release from the ER is excited at particular subcellular ... that the cells oscillating faster in isolation peak before the slower cells Thus the intercellular coupling leads to a (near) : entrainment, or synchrony, of the oscillations in adjacent cells On...

Ngày tải lên: 24/03/2014, 00:21

... average gene expression of a culture of isogenic cells and the gene expression of a single cell within such a culture [15,16] Therefore, single cell analysis with high spatiotemporal resolution ... processes within a cell Single cell analysis is not yet widely applied mainly because of the fact that the technologies that are needed are not fully developed The fact that microbial cells are much ... mRNAs per cell [22] Assuming that these mRNAs comprise 5% of the total RNA [20,23] and that the average RNA length is 2,500 nucleotides [20], S cerevisiae would contain 2.5 pg total RNA per cell...

Ngày tải lên: 09/08/2014, 23:20

... detection of single EBV-transformed cells using the GADA algorithm Single- cell CNV detection of all seven EBV-transformed cells Each row represents the profile of one EBV-transformed cell and each ... contributions JRV designed the novel single- cell experiment and critically reviewed the manuscript EV and TV designed the novel single- cell experiment, carried out the single- cell experiments and generated ... number variation detection of single EBV-transformed cell 1168 using existing Page 12 of 14 normalization methods (a-d) Single- cell CNV detection of EBVtransformed cell 1168 after global loess...

Ngày tải lên: 09/08/2014, 23:20

... tumbling frequency and the protein-localization dynamics When the cell divided into two daughter cells, one of these two daughter cells was picked up by optical tweezers, transported to the axle ... environmental change Authors' contributions II carried out the microchamber design, cell preparation, single cell observation, image analysis and drafted the manuscript DS and IK prepared Tar-GFP ... culture system for observation of isolated individual cells Lab Chip 2001, 1:50-55 Wakamoto Y, Inoue I, Moriguchi H, K Yasuda: Analysis of singlecell differences using on-chip microculture system...

Ngày tải lên: 11/08/2014, 00:22

... continuous light The cells used for on-chip cultivation were taken from the culture in the early-log phase (≈104 cells/ml) On-chip single- cell cultivation system The on-chip single- cell cultivation ... the absolute cell volume, whereas ours is based on the relative cell volume (a ratio of the current cell volume to the initial cell volume) Discussion To investigate cell growth and cell cycle ... dynamics of single- cell gene expression over multiple cell cycles Mol Syst Biol 2005, 1, 2005.0024 13 Wakamoto Y, Yasuda K: Quantitative evaluation of cell- to -cell communication effects in cell group...

Ngày tải lên: 11/08/2014, 00:22

... target cells In patient with multiple myeloma, for example, routine FISH assessment may yield normal results owing to the low percentage of diseased plasma cells Thus, a method to generate single- cell ... diagnostic of mosaicisms or in heterogeneous cell populations Here, we describe the production of specific painting probes from a single avian microchromosome and a single chromosomal region using the ... traditional cytogenetic methods (Figure 2) Here, we show that DOP-PCR can be applied to a single unstained chromosome or a single chromosomal region without topoisomerase treatment normally used in the...

Ngày tải lên: 11/08/2014, 00:23

... sequence up to 25 single cells on a single- flow cell lane, thus allowing 200 single cells to be profiled in a single run Moreover, by decreasing the genomic resolution of each single- cell copy number ... of cells present, but further to obtain genetic information from the rare cells that may be the most malignant Isolating single cells To study a single cell it must first be isolated from cell ... Methods for single- cell genomic profiling Several methods have been developed to measure genome-wide information of single cells, including cytological approaches, aCGH and single- cell sequencing...

Ngày tải lên: 11/08/2014, 12:21

... stage and this can obscure the fine detail of the cycle Single cell measurements may help here Single cell analysis in yeast Returning to single cell analyses of fission yeast, volume growth was followed ... over several cell cycles starting with single cells – a remarkable achievement In both cases, the results were elegant linear patterns with an RCP at division Another interesting single cell method ... pictures J Cell Comp Physiol 1932, 1:387-407 Mitchison JM: Growth during the cell cycle Int Rev Cytol 2003, 222:165-258 Mitchison JM: The growth of single cells Schizosaccharomyces pombe Exp Cell Res...

Ngày tải lên: 13/08/2014, 22:22

... that single cell studies are more misleading than population studies Understanding cell growth should be based on cell culture behavior rather than single cell studies It is also argued that singlecell ... during the cell cycle Int Rev Cytol 2003, 226:165-258 Mitchison JM: The growth of single cells II Saccharomyces cerevisiae Exp Cell Res 1958, 15(1):214-221 Mitchison JM: The growth of single cells ... published data Figure in Growth length of a single wild-type cell of S pombe Growth in length of a single wild-type cell of S pombe The data for the cell lengths from Figure of Sveiczer et al...

Ngày tải lên: 13/08/2014, 23:20

... genes in epithelial cells derived from the human SW620 colon cancer cell line Cells were harvested from two plates of cell culture that each contained approximately × 107 cells For the first ... only been a single published report of the variability of gene expression in single cells, which did not provide an underlying statistical model for mRNA representation within the cell [1] While ... (a) plat e of ~1 x cells ce ll s 10 s a m p l e s o f 1x10 1x1 cells 1x1 10 s a m p l e s o f 1x10 cells 10 s a m p l e s o f 1x1 1x10 cells 10 s a m p l e s o f 1x1 1x10 cells (b) 120 m g o...

Ngày tải lên: 14/08/2014, 17:22

Ngày tải lên: 14/11/2014, 06:13

... in single cell studies 2.2.1.4 Cell- cell interactions Besides varying the cell geometry and size, there is also great interest in understanding how cell- cell interactions could affect intracellular ... allow a single cell- cell contact to be formed between two triangular-shaped cells as shown in Fig.2.3.b By comparing a single cell of similar shape and size to a cell pair in the bow-tie, cell response ... understanding how cell- cell interactions can affect cell behavior With an excellent physical control of the geometry, adhesion and arrangement of single cells, systematic investigation of global cellular...

Ngày tải lên: 09/09/2015, 11:30

... Nickel 95 4.9 Cell recovery and cell passage protocols The cell recovery is the method for recover the cell from being kept alive at very low temperature (-150oC) The freeze cells were immediately ... image of cells shows green-fluorescent cells which uptook SYTOX® Green and (c) fluorescent image of cells shows redfluorescent cells which uptook DEAD RedTM (b) and (c) were taken after cells were ... polar molecules into a host cell through its membrane by giving quick electrical pulses across the cell For this thesis, the design and fabrication of a novel single cell electroporation biochip...

Ngày tải lên: 11/09/2015, 10:16

... for single cell electroporation Single- cell electroporation and pulse parameter optimization The electroporation for single N2a cells was performed Preliminary experiment showed the successful cell ... electroporation 4.3.1 Cell preparation We used mouse Neuroblastoma N2a cells (ATCC 131, American Type Culture Collection) in the single cell electroporation study Neuroblastoma cells are used as ... incubator at 37 °C with 5% CO2 The cell recovery and cell passage protocols are described in figure 4.9 For cell recovery, first the cells were recovered from -150 oC in a cell culture flask (25 cm2)...

Ngày tải lên: 11/09/2015, 10:16

... VOUT For a single cell input, 1.8 V output applications, it is recommended that the inductor is changed from 4.7 µH to 2.2 µH and the output capacitor is changed to 20 µF For single cell inputs, ... MCP1640/B/C/D family of devices enables operation from a single cell input, delivers high efficiency, is small in size, and provides excellent dynamic performance Like most DC-DC converters, the ... Sleep mode current, this can result in substantial average battery current For some multi -cell or coin cell applications, a Bypass mode that uses the integrated P-Channel MOSFET to connect the...

Ngày tải lên: 11/01/2016, 16:55

... 293T/CD20 cell line, but not to the control 293T cell line In contrast, the lentiviral vector FUW/VSVG was able to bind to both 293T and 293T/ CD20 cell lines In addition, a quantitative virus -cell ... transfected cells Solid line, analysis on transfected 293T/CD20 cells; shaded area, analysis on 293T cells (B) Analysis of co-expression of the FM and antibody on gated GFP-positive cells FM was ... cytometry was then used to analyze the treated cells days posttransduction Targeted transduction of 293T/CD20 cells Virus -cell binding assay 293T/CD20 or 293T cells were incubated with mL of lentiviral...

Ngày tải lên: 12/08/2014, 04:21

... CHO-K1 cells Cells were allowed to grow for 10 days before protein expression was analysed Cell fusion Cell fusion was performed in two individual steps (Fig 1) by mixing equal number of cells ... prewarmed medium and incubated for The cells were centrifuged, the supernatant discarded and growth medium added to achieve a cell concentration of · 105 cellsÆmL)1 The cells were allowed to recover for ... transfected cells were screened by immunofluorescence staining CHO-K1 cells transfected with J-chain were cultured on micro slides Cells were fixed and permeabilized in methanol ()20 °C for min) The cells...

Ngày tải lên: 18/03/2014, 01:20

... sorting (FACS) Unlike phage display, FACS has the distinct advantage of relying on real-time quantitative multiparameter analysis of individual cells, allowing single- cell resolution for selection ... of E coli Jude-1 ⁄ F¢ cells carrying plasmid pBAD33–ZZ, yielded an output of 1.5 · 106 cells harboring phagemid pMAZ360–IgG The cells were induced for the expression and display of IgG, and subjected ... phage display to receive FDA approval, and at least 16 human mAbs derived from phage display are currently in advanced clinical trials for a wide range of human diseases [29,30] Phage display...

Ngày tải lên: 22/03/2014, 21:21