... containing the C/EBPs consensus sequence was increased by LPS and LPS+IFNg treatments (Figure 3A, lanes 1-3) Supershift experiments showed the presence of C/EBPb in shifted complexes I Page of 15 to III ... expression of NOS2 and IL-1b Results are expressed as relative fold units of ΔΔCt value between gene of interest and actin + Rn1 8s as reference genes Two-way ANOVA, followed by Bonferroni s test ... simultaneous increase in activating and inhibitory C/EBPb isoforms is a common observation EMSA analysis with supershift experiments showed the presence of C/EBPb in bands I, II and III These bands may contain...
Ngày tải lên: 19/06/2014, 22:20
... invertebrate species is less well studied; RGD sequences have been found in species as diverse as sea urchins and amoebae, however, and integrins and their biochemical functions are likely to ... cytoskeleton and from the dynamic environment (that is, shear forces within blood vessels) We are just beginning to understand the structural and chemical basis of this sensoreffector system A particularly ... reports on the environment in which the cell finds itself Thus, integrins serve as both sensors of their molecular surroundings and effectors that conduct motile forces exerted by the cell s cytoskeleton...
Ngày tải lên: 14/08/2014, 07:21
Fish species distribution in seagrass habitats of chesapeake bay are structured by abiotic and biotic factors
... necessary to fulfill statistical assumptions because statistical significance is assessed with randomization tests and relationships between the response and explanatory data matrices are easily ... These threats endanger not only the seagrasses, but also the associated fish species that rely on these habitats Numerous investigations have quantified fish associations and changes in assemblages ... Sobocinski et al 2013) have examined assemblages associated with seagrass beds Those that have sampled fish on seagrass beds have posed single-species hypotheses (Dorval et al 2005b, 2007; 115 Smith...
Ngày tải lên: 04/09/2015, 12:54
Báo cáo đề tài nghiên cứu khoa họcNGHIÊN CỨU DAO ĐỘNG CỦA CÁP VĂNG DƯỚI TÁC DỤNG CỦA GIÓ MƯA KẾT HỢPTHE RESEARCHING ON VIBRATIONS OF STAY CABLES ARE INDUCED BY WIND AND RAIN INTERACTION
... S sin( ωt ) + S3 cos(ωt ) + S sin( 2ωt ) + S5 cos(3ωt ) (2.15) Với: U- Vận tốc gió; C1 = L1.cosγ + D1 .sin γ ; C2 = L2 cos γ + D2 sin γ ; γ − θ i = α S = − U D ρ C1 am ; 2 S3 = URD ρ am cos ... văng sau: y ( x, t ) = X ( x )T (t ) = sin ( + + S2 ω.(2mξ s ) cos(ωt ) − 2 .S5 m.(16.ω − 9.ξ s ) Với : n.π L S3 x ).{e − ξ t P1 cos S ω.(2mξ s ) cos(3ωt ) + sin( ω t ) + ( ) ω − ξ S t + P2 sin ... là: sin α sin β U = U cos β + sin α sin β ; γ = ε sin cos β + sin α sin β 2 −1 ÷ ÷ (2.5) Để phản ánh ảnh hưởng góc gió công điều kiêôn trời mưa vế phải phương trình (2.5) ta bổ sung...
Ngày tải lên: 25/05/2016, 21:22
Card Tricks And The Way They Are Performed
... "Position as to Suits" Every card of the same denomination is thir, teen cards apart and runs in the same order of suits as in the system, viz.: Hearts, Spades, Dia monds, Clubs Next | Previous ... Previous | Contents THE SYSTEM In stacking the cards lay the of hearts down face up, the of spades on top of the of hearts and so on to the end, reading across Hearts Ace King Queen Jack 10 Spades ... does not enter into this system Next | Previous | Contents Si Stebbins Card Tricks Next | Previous | Contents RULES RULE "Shuffling" In shuffling never riff or mix cards in A person may take as...
Ngày tải lên: 19/03/2014, 13:04
Báo cáo khoa học: Differences in substrate specificities between cysteine protease CPB isoforms of Leishmania mexicana are mediated by a few amino acid changes potx
... Additional S and S subsites are not listed because they are so far not identified by the cocrystallization of a peptide substrate and the proteases of the parasites Protease S2 subsite S1 subsite Catalytic ... (Sigma) as the substrate Activity analyses of cysteine proteases using gelatin SDS/PAGE Parasite cysteine protease activities were analysed using substrate SDS/PAGE as described previously [7,28] ... substrate specificity is defined by the binding affinities of the subsites The catalytic residues and the S1 and S1 ¢ subsites are highly conserved between the three parasite species, and key residues...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo toán học: "The centers of gravity of the associahedron and of the permutahedron are the same" ppsx
... partition its vertices into isometry classes of triangulations, which parameterize the vertices, and we show that the center of gravity for each of those classes is the center of gravity of the permutahedron ... triangulation of P is a set of n noncrossing diagonals of P Let us be more explicit A triangle of P is a triangle whose vertices are vertices of P Therefore a side of a triangle of P is either an ... of boundary edges of P between Ai and Aj passing through vertices indexed by smaller numbers than j with the number of boundary edges of P between Aj and Ak passing through vertices indexed by...
Ngày tải lên: 08/08/2014, 12:22
Báo cáo y học: "erum levels of soluble receptor for advanced glycation end products and of S100 proteins are associated with inflammatory, autoantibody, and classical risk markers of joint and vascular damage in rheumatoid arthritis" doc
... inflammatory diseases such as RA, psoriasis and inflammatory bowel disease [2125] Longitudinal and cross-sectional studies suggest a relationship between S1 00A12 and RA disease activity [26-28] The S1 00A12 ... atherosclerosis by high levels of S1 00A12 in hemodialysis patients [74] However, this is the first time such an association has been shown with CVD and RA Conclusions sRAGE and S1 00 proteins were ... and space occupying lesions Peripheral vascular disease was confirmed if Doppler ultrasonography showed significant large vessel disease Cigarette smoking was assessed by questionnaire, which...
Ngày tải lên: 09/08/2014, 13:22
Default Risk Cannot Explain the Muni Puzzle: Evidence from Municipal Bonds That Are Secured by U.S. Treasury Obligations ppt
... Includes U .S. -based and non-asset-backed corporate issues Includes only GNMA, FNMA, and FHLMC mortgage-backed securities Rough estimates by Moodys’ Investor Services Sources: Public Securities Association, ... OLS relies upon the standard OLS assumptions, which in this case imply that the cross-time and cross-term-structure errors are uncorrelated and homoscedastic A likelihood ratio test rejects the ... purposes Despite these benefits, problems common to most studies of municipal bond yields persist State taxes are very difficult to control for because of the heterogeneity among issuers and investors...
Ngày tải lên: 15/03/2014, 03:20
A survey of technology thinkers and stakeholders shows they believe the internet will continue to spread in a “flattening” and improving world. There are many, though, who think major problems will accompany technology advances by 2020 doc
... interested institutions was sought, this survey should not be taken as a representative canvassing of internet experts By design, this survey was an “opt in,” selfselecting effort That process does ... Project Scenario Two: English displaces other languages languages, making a single language less necessary 2) As in all evolutionary systems, very successful, dominant species spawn subspecies; English ... difficult and nearly impossible There are scenarios where processes based on automation and intelligence based on rules and identities will miss 'outliers' and 'exceptions' thereby resulting in mistakes,...
Ngày tải lên: 29/03/2014, 19:20
the university of north carolina press opium war 1840-1842 barbarians in the celestial empire in the early part of the nineteenth century and the war by which they forced her gates mar 1998
... feel as keenly as I the pleasure of sometimes seeing growing green things." To Williams and most of the residents these back houses seemed like prisons, dark, cramped, and in summer impossibly stuffy ... Goodwin Sands and destroyed it), the consequent cession to the Chinese of the Isle of Wight and a strip of the mainland along Southampton Water, the irresistible demand of the Japanese and the Straits ... list'' of world records: the largest per capita number of Rolls-Royces, MercedesBenzes, and BMWs, "the highest consumption rate of VSOP cognacs, the highest levels of stress, the highest prices...
Ngày tải lên: 11/06/2014, 14:18
Báo cáo y học: "B cells from rheumatoid arthritis patients show important alterations in the expression of CD86 and FcgRIIb, which are modulated by anti-tumor necrosis factor therapy" potx
... therapy and its impressive results in reducing symptoms and preventing disease progression [27] Studies on murine models have suggested that antigen-specific B cells are required as APCs for the ... naïve cells, memory cells and plasmablasts by means of CD19 and CD27 staining, we detected similar percentages of these subsets in the two groups of individuals (Figure 1) Higher frequency of activated ... cells from RA patients and controls expressed similar levels of this receptor, its expression was significantly lower on RA memory B cells and plasmablasts (P = 0.0005 and P = 0.0013, respectively)...
Ngày tải lên: 12/08/2014, 12:20
Tài liệu Báo cáo khoa học: Regulators of G-protein signalling are modulated by bacterial lipopeptides and lipopolysaccharide pptx
... regulation of RGS1 and RGS2 expression Results RGS1 and RGS2 mRNA expression is regulated by LP and LPS BMDM were stimulated with FSL-1, a ligand for the TLR2 ⁄ heteromer, and LPS, a ligand for ... demonstrate that both BMDM and J774 cells express RGS1 and RGS2 mRNA and modulate expression after stimulation with LP and LPS Expression patterns of RGS1 and RGS2 after activation of TLR3 and ... various culture times, mRNA was isolated and the expression of multiple probe sets was analysed by microarray analysis According to the microarray data, only 11 of 18 tested RGS mRNAs are expressed...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: The distinct nucleotide binding states of the transporter associated with antigen processing (TAP) are regulated by the nonhomologous C-terminal tails of TAP1 and TAP2 ppt
... (2001) Distinct functions of the ATP binding cassettes of transporters associated with antigen processing: a mutational analysis of Walker A and B sequences J Biol Chem 276, 22107–22113 14 Lapinski, ... h and the membrane pellets resuspended in 800 lL of 0.25 M sucrose buffer Afterwards, 5.6 mL of 2.5 M sucrose gradient buffer was added and the suspension overlaid carefully with 2.9 mL of M and ... (A) Steady state expression of TAP chimera 2DV (left panel) T2 transfectants expressing single TAP2 or TAP2DV were lysed in buffer containing 1% Triton X-100 Lysates were separated by SDS/PAGE and...
Ngày tải lên: 20/02/2014, 02:21
Tài liệu Báo cáo Y học: Phosphatidylinositol synthesis and exchange of the inositol head are catalysed by the single phosphatidylinositol synthase 1 from Arabidopsis docx
... synthase placed in a microbial environment is capable of catalysing several enzymatic reactions: PtdIns de novo synthesis, using CDP-DAG and free inositol as substrates and producing PtdIns and ... analysis of radioactive PtdIns molecular species The exchange reaction was also studied by analysis of the radiolabelled PtdIns molecular species produced using various PtdIns substrates (Fig 1) This ... PtdIns molecular species were analysed by radio-HPLC as described previously [17] RESULTS Enzymatic activities catalysed by AtPIS1 Previously published results indicate that the yeast PtdIns synthase...
Ngày tải lên: 22/02/2014, 04:20
Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... than by SUR2A [6] In this study, we tested this hypothesis explicitly, by measuring the ATPase activity of full-length SUR2A and SUR2B, and that of their isolated NBDs Results Figure 1A shows SDS ... analysis of purified fusion proteins consisting of maltose-binding protein (MBP) linked at its C-terminus to one of the NBDs of SUR2 (MBP-NBD fusion proteins) Figure 1B,C shows SDS/PAGE analysis of ... tag was inserted into rat SUR2 between Ala1026 and Asp1027 Full-length SUR2A and SUR2B were expressed in insect cells (Sf9), using a baculovirus expression system (Invitrogen, Paisley, UK), and...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... analysed, penaeidin mRNAs were detected in circulating haemocytes in blood vessels and sinuses and in cells present within most tissues The shape of the positive cells suggests that they are infiltrating ... investigate the ability of tissues other than haemocytes to express penaeidins and to study the distribution of both transcripts and peptides in response to challenge, shrimp tissues were analysed ... performed using a BIOCOM system Results are expressed in arbitrary units (AU) The level of expression was quantified in 25 cells per slide and five slides per shrimp and each value represents the average...
Ngày tải lên: 18/03/2014, 01:20
Cancer Research UK’s strategy 2009–2014: Cancer Research UK’s aim is to reduce the number of deaths from cancer. Our future plans are ambitious, but they are in line with the challenge and the responsibility we face. docx
... 4,500 scientists, doctors and nurses throughout the UK We fund research carried out in our own Institutes, as well as awarding grants to researchers based in universities and hospitals We are also ... understanding of the disease Increasingly we are using our website to make information easily accessible, using different formats, such as our science blog We will increasingly use both video and ... huge steps forward in improving survival and in preventing thousands of new cases of the disease, both in the UK and across the world Cancer Research UK has been at the heart of this progress thanks...
Ngày tải lên: 22/03/2014, 16:21