similar dose dependent damage in the nuclear and mitochondrial genomes

... exist in the regulation of copper and iron metabolism genes in budding and fission yeast, in particular in their responses to copper toxicity Our findings also provide new insights into the coregulation ... accumulation by initiating a general stress response, we were interested to determining whether S cerevisiae has retained the ability to induce a similar response in the absence of the specific ... these motifs is sufficient to confer iron dependent regulation by Fep1p [42] interactions tion into the Fio1p oxidase In the absence of a putative Fep1p binding site in the promoter region, the...

Ngày tải lên: 14/08/2014, 07:21

... former, the RNA passesthrough the 5.7MCsCl but proteins and large DNA molecules are excluded In the latter method, protein and DNA partition into the acid phenol phase leaving the RNA in the aqueous ... sedimented to the bottom of the tube Mononuclear cells, including lymphocytes form a distinct, cloudy band within the clear solution at the interphase of the upper sample plasma layer and the lower ... these bands indicates the high-quality of the RNA minipreparations After RT-PCR, the PCR-products were run in a 1.5% agarose gel and stained with ethidium bromide The arrow indicates the mdrl...

Ngày tải lên: 11/04/2014, 10:30

... scaled value based on the distribution within each dataset (2003 and 2004, independently) Cyclophilin, β-tubulin, and EF1-α (m) were excluded since they were not included in all of the evaluation methods ... Given that cyclophilin, β-tubulin and EF1-α (m) were excluded from the final analyses (Table 3), they could not be included in the final scoring scheme For all other genes, the results from each ... collaborative research and development initiative The authors also wish to thank the Province of British Columbia and the BC wine industry for their continued support of research activities in the University...

Ngày tải lên: 12/08/2014, 05:20

... of being easy to culture and maintain and they are potentially valuable in the bioconversion of keratinous wastes; in the detergent, fertilizer, biopolymer, pharmaceutical and animal feed industries; ... hydrolyze insoluble hard proteins more effectively than other proteases such as trypsin, pepsin and papain The genus Bacillus provides most of the keratinase enzyme with commercial value, offering the ... significant source of protein for livestock because of their high protein content (>85% CP) Feathers contain large amounts of cysteine, glycine, arginine and phenylalanine Raw feathers, however, are...

Ngày tải lên: 06/10/2015, 12:58

... temperature inhibited the growth and keratinase production of the bacteria, so the extent of feather degradation will be rather low These results are similar with that of other C indologenes (Sangali and ... significantly at the third day In this stage, the keratinase production occurred strongly because of the adaptation of bacteria in the medium and using the nutrients of the medium with the biochemical ... phase On the other hand, the feather degradation capability increased significantly from the third day and continued to raise slowly to the seventh day These results are dissimilar with the report...

Ngày tải lên: 06/10/2015, 13:04

... effort to reduce the grazing pressure by sea urchins in the barren grounds in the inner areas of the bay MATERIALS AND METHODS Study site The study was carried out in the inner and outer areas ... flora and infauna in the outer and inner areas of the bay A total of 16 macrophyte species in 2003 and 15 species in 2004 were observed in Line (Table 1) U pinnatifida and crustose coralline algae ... that there were areas under high water flow conditions preventing the migration and/ or grazing by sea urchin even with their high abundance in the outer bay In contrast, in the inner areas (Line...

Ngày tải lên: 05/09/2013, 09:38

... protein cho Mật rỉ đường 20 kg/ha/ngày 4-5 ngày Chuyển đổi tảo thành vi khuẩn ổn đònh Photograph: Dr Ponlert Chandratchakun Cách Cho n nh Hưởng Đến FCR FCR Variation at 174 farms in Thailand ... ngày – tôm sinh trưởng tốt – Không ô nhiễm nước – Phù hợp nhòp sinh học tôm sản sinh men tiêu hoá Thí nghiệm bể, cần kiểm nghiệm thực tế ao nuôi Tiết kiệm nhân công CẢM ƠN SỰ QUAN TÂM THEO DÕI CỦA ... - 147 180 225 271 332 Africa 10 - - - - - China 10 140 188 252 266 293 Thailand 300 280 240 120 80 - - 10 190 245 Malaysia 18 18 19 20 20 - - - - - Indonesia 94 104 113 90 80 33 80 Vietnam 137...

Ngày tải lên: 19/01/2014, 17:20

... not speciate in close proximity, as the populations in Lakes Ohnuma and Akanko (sites 29 and 30) were thought to have been introduced from the island of Honshu in Japan (Ban and Minoda 1989) Morphological ... within lineages has been found in other microcrustaceans, such as within the genus Daphnia (Colbourne and Hebert 1996) The relatively large morphological variation within lineages has led to the ... (Felsenstein 1981; Huelsenbeck and Rannala 1997) was performed on the 16S rRNA data to determine whether the assumption that substitutions in the data evolved in a clocklike manner was violated and...

Ngày tải lên: 13/02/2014, 15:20

... missing in maxilla in 23% and in mandible in 40% of the subjects; the corresponding figures for premolars were 20% and 13% Table 5.3 shows the distributions of the subjects according to the clinical ... brushing in the evening, 79% in the morning, and 17% after a meal; 19% of the subjects indicated daily interdental cleaning Of all the subjects, 57% reported using fluoride toothpaste always; the ... been included in routine care for elderly subjects in the Nordic and other industrialized countries According to the scarce reports from Lithuania and other countries, now undergoing changes in their...

Ngày tải lên: 13/02/2014, 18:20

... protein (FMRP) and the G-quadruplex DNA-binding motif in nucleolin [21– 27] The RGG domain of FMRP, which is an RNAbinding protein involved in nerve cell differentiation, interacts with the G-quartet ... (Fig S6) These findings indicate the importance of the guanidinium group of the Arg in RGG3 for binding to the G-quadruplex In conclusion, EWS appears to be a DNA- and RNA-binding protein that ... G-quartet forming RNA [22–25] In addition, the RBD and the RGG domain of nucleolin, a DNA-binding protein contributing to the transcription of ribosomal RNA, bind to the G-quadruplex forming ribosomal...

Ngày tải lên: 15/02/2014, 01:20

... Mainz, the leading favourer of the New Learning amongst the prince-prelates, and it was mainly from the Humanist standpoint that he regarded the beginnings of the Reformation After leaving the ... undermining the [9]whole structure The growing use of firearms in war; the rapid multiplication of printed books; the spread of the new learning after the taking of Constantinople in 1453, and the ... College, or the seven Electoral Princes, forming their head These constituted the feudal "estates" of the empire Then came the "King of the Romans"; and, as the apex of the whole, the Pope in one...

Ngày tải lên: 16/02/2014, 04:20

... seen in the presence of Cu(II) and H2O2 Fragmentations occurred predominantly at glycosidic bonds between the rings and in the amide bond, linking the aglycon to ring B (see arrows at Fig and ... complex), through the relaxed form II, to the linear form III In the Cu(II) + H2O2 system, the amount of form II increased during the duration of the experiment Only for the complex in the presence ... Ami and Cu(II)-Ami complex induced highly specific cleavage in the anticodon loop, at the hipermodified base Y37 (wybutine) (Figs and 7) Also other aminoglycosides, as kanamycin A, neomycin B and...

Ngày tải lên: 21/02/2014, 01:21

... knowledge of their rights, including those stipulated in existing health and safety regulations, and therefore face great difficulties in exercising them The information presented in this article ... The decrease in the number of labour inspections carried out on tree plantations is inversely related to the continuous increase in the area of land planted and the volume of wood harvested The ... Swaziland, Uganda, India, Indonesia, and Thailand are examples The answer to these questions should be plain to see, but unfortunately those who control the propaganda machine of the global pulp and...

Ngày tải lên: 21/02/2014, 04:20

... and mss are the intercepts and slopes of the lower and upper baselines of the melting curves, respectively; T is the temperature of interest in Kelvin, a is the molar fraction of strands in the ... sets of the temperature -dependent thermodynamics for the DNA/DNA and RNA/DNA oligonucleotide duplexes In the present study, we determined the temperatureindependent and temperature -dependent thermodynamic ... ị P Temperature -dependent thermodynamic analysis The differences in hydration between the structured duplex strand and the coiled single strands gives rise to an increase in the heat capacity...

Ngày tải lên: 22/02/2014, 07:20

... because in this case of open rigid string we encounter a coincidence of the following two mathematical obstacles: the presence of the high derivatives in the Lagrangian , and the fact that the field ... (53) The boundary terms (the last three terms on the right hand side of formula (50)) vanish in the case of closed string In the case of open string they give a non-vanishing contribution even in ... boundary of the rectangle Ω The advantage of the form of the variation δS is that it involves the least possible number of derivatives of the variations δxµ The remaining derivatives of δx in formula...

Ngày tải lên: 05/03/2014, 14:20

... of the G-tetrad core, (b) groove binding, (c) taking place of one or more strands in the core, (d) interacting with the backbone (core and loops), and (e) interacting with the loop bases A ligand ... strand and only the 3¢-end G-tract of the other strand [30] (Fig 3D) The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one strand oriented in the ... evidence supporting the intercalation of a planar ligand between G-tetrad layers In addition to the end-stacking binding mode of the aromatic rings, some ligands also contain other moieties that...

Ngày tải lên: 06/03/2014, 09:22

... according to their genomic context: exonic miRNA in noncoding transcripts, intronic miRNAs in noncoding transcripts, and intronic miRNAs in protein-coding transcripts [8,9] Based on the accumulating knowledge ... Improved strategy for inserting specific artificial miRNA sequences into the targeting vectors The terminal regions of the arms of the miR155 (A) and miR30 (B) vectors are shaded, and their stems designed ... composed of the 5¢ donor site from the first intron of the human b-globin gene and the branch and 3¢ acceptor site from the intron of an immunoglobulin gene heavy chain variable region (derived from...

Ngày tải lên: 07/03/2014, 11:20

... Mainz, the leading favourer of the New Learning amongst the prince-prelates, and it was mainly from the Humanist standpoint that he regarded the beginnings of the Reformation After leaving the ... stigmatizes them as "unchristian, God-forsaken folk, whose hand is ever ready in striking, stabbing, robbing, burning, slaying, gaming, who delight in wine-bibbing, whoring, blaspheming, and in the making ... their main, and in many cases their only, source of revenue; and hence, abandoning the hope of independence, they threw in their lot with the authorities, the princes, lay and ecclesiastic, in...

Ngày tải lên: 08/03/2014, 13:20