silicon based nano devices for detection of dna molecules

... DEVELOPMENT OF GOLD NANOPARTICLE -DNA NANOSTRUCTURE ASSEMBLY FOR DETECTION OF DNA, RNA AND PROTEIN BIOMARKERS SEOW NIANJIA (B.Eng (Hons), National University of Singapore) A THESIS SUBMITTED FOR THE ... theme of biomarker detection with AuNP nanostructural assemblies, this thesis is made up of a number of unique works: To develop a nanostructure system for the detection of different mutations of ... the formation of dimeric nanostructure with DLS for the detection of the ER protein The presence of the ER-binding site on the dimers allowed the binding of the ER, and which formed the basis for...

Ngày tải lên: 09/09/2015, 11:16

... coordination of the study and drafted manuscript Page of doi:10.1186/1556-276X-6-385 Cite this article as: Prasek et al.: Carbon composite micro- and nanotubes -based electrodes for detection of nucleic ... use and rapid isolation and detection of a viral nucleic acid by using paramagnetic microparticles and carbon nanotubes -based screen-printed electrodes Microfluid Nanofluid 2010, 8:329-339 17 Huska ... still one of the most sensitive analytical technique voltammetric methods can be considered as a suitable tool for detection of nucleic acids We show the successful application of modern nano- technologies...

Ngày tải lên: 21/06/2014, 03:20

... characterization of electrical porous silicon based sensors for their use in biodetection She carried out her research at the Center for Future Health under the supervision of Professor Philippe ... Simulation of the electrostatic field distribution in the cross section of the c-Si substrate with a uniform line of charge of 34 nC on the top surface A dc voltage of V and an ac voltage of V were ... induced by the presence of molecules This effect was simulated by considering a uniform sheet of charge on the top surface in the presence of a dc voltage of V and an ac voltage of V between the electrodes...

Ngày tải lên: 16/03/2014, 15:23

... imprinted polymer for detection of sialic acid Biosens Bioelectron 16, 1059–1062 Wu AH & Syu MJ (2006) Synthesis of bilirubin imprinted polymer thin film for the continuous detection of bilirubin in ... dampening of the resonator caused by the viscous Acoustic biophysics Acoustic biosensors allow the label-free detection of molecules and the analysis of binding events In general, they are based ... 5473 Detection of low molecular mass analytes Y Uludag et al ˘ For all the examples cited above, the binding capacity of the sensor surface is critical to maximize the sensitivity of the sensor for...

Ngày tải lên: 23/03/2014, 07:20

... estimation of the performances of the DVB-T signals detection algorithm These performances have been estimated in several contexts leading to the Pierre Jallon 1 0.9 Good detection probability Good detection ... probabilities of the algorithm are illustrated in Figure for η = 1/4 and in Figure for η = 1/32 Several choices of Nb have been tested to illustrate the impact of this parameter on the performances of the ... choice of Nb We hence evaluate in this section the impact of this parameter on the mean and on the variance of J y (Nb ) under both assumptions NUMERICAL EVALUATION OF THE PERFORMANCES OF THE...

Ngày tải lên: 22/06/2014, 06:20

... valuable overall picture of the bacterial DNA environment present in the SF of the actively inflamed joints of arthritis patients Characterization of the DNA reveals a wide spectrum of organisms so far ... well as cloning and sequencing of the entire 16S rDNA to identify any bacterial DNA potentially present in the SF samples of patients with ReA, UA, and other forms of arthritis who were also analyzed ... fragments of DNA were amplified Page of 11 (page number not for citation purposes) Arthritis Research & Therapy Vol 11 No Siala et al Table Bacterial species identified by sequencing of cloned 16S rDNA...

Ngày tải lên: 09/08/2014, 14:22

... trait carriers before they develop cancer To establish proof of principle for this assay, we analyzed fresh blood samples from a population of individuals who are at high risk for having a germline ... 10 ug of total cell protein (C) Detection of MLH1 and MSH2 proteins on western blots with a mixture of varying amounts of HCT116 and LoVo cell lysates Results show that the combinations of these ... risk for hereditary colorectal cancer The Journal of Laboratory and Clinical Medicine 2004, 143:59-66 Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein...

Ngày tải lên: 10/08/2014, 10:21

... recovery efficiency of DNA is better than that of RNA Therefore, we evaluated the sensitivity of detection of DNA- or RNA -based junctional biomarkers in samples extracted from formalin-fixed cells ... possibility of a BCR-ABL translocation Comparison of sensitivity: DNA or RNA Because a clinical sample is not uniformly composed of malignant cells, we next evaluated the sensitivity of detection of the ... sensitivity of 0.03% for the primary detection of a BCR-ABL fusion We also evaluated the sensitivity of detection of the PCR product spanning the chromosome junction for molecular follow-up of the disease...

Ngày tải lên: 11/08/2014, 12:21

... nanobiosensor has been extended for the detection of ssDNA target of genomic DNA of Legionella pneumophilla [75] and complementary DNA (cDNA) detection of genomic DNA of dengue virus (DENV1) [76] ... property of redox species The non pcr based technique of DNA detection require minimal sample preparation step for analyte detection and show very low detection limit Among non pcr based DNA detection, ... membrane -based label free DNA biosensor is developed for detection of target DNA strands of Legionella sp using 5'-aminated DNA probes immobilized into the nanochannels of alumina Alumina nanoporous xv membrane-like...

Ngày tải lên: 08/09/2015, 18:11

... further research before we can eventually come out with an EEG -based emotion monitor For detection of anxiety emotion by EEG measurement, an Independent Component Analysis (ICA) based energy spectrum ... both sides of the anterior temporal, iv Summary the number of independent components for anxiety status was 50% to 100% higher than that of emotion void status The ability of this ICA -based method ... and C value of SVM during optimization 71 vii List of Tables LIST OF TABLES Table 4.1 Function of components of limbic system 34 Table 4.2 The conductivity (S/m) of tissues below...

Ngày tải lên: 22/10/2015, 21:19

... pairs The template in the different lanes is: cDNA for the photosynthetic isoform (lanes 1), cDNA for the non-photosynthetic isoform (lanes 2), cDNA synthesised from total mRNA extracted from ... thaliana AtGS1 isoform AY088312 Arabidopsis thaliana Arabidopsis thaliana AtGS1 isoform AtGS1 isoform AY059932 AK118005 AC# (amino acid) Q56WN1 70% Q9LVI8 Plant Oryza sativa OsGS1 isoform AB037664 ... Algorithms for Molecular Biology 2010, 5:24 http://www.almob.org/content/5/1/24 Page 14 of 16 Figure Analysis of two Pinus pinaster gene isoforms The specific primer pair for the photosynthetic isoform...

Ngày tải lên: 20/12/2015, 08:16

... of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 IDEAL LT8600LT The IDEAL LT8600 performs ... on the determination of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 MICROTEST OMNIScanner2™ ... PC Software: Software V 7.0 Accessories: § Permanent Link Interface Adapter 8262-38: Includes: § § interface adapters, suitable for the KRONE KM8 Notes on the use of hand-held test devices for...

Ngày tải lên: 21/12/2013, 18:15

... combination of bands 5, and which is of great efficacy for the aims of our analysis In Figures and the set of bands 5, and are depicted respectively for the month of May 1994 and March 1997 The fusion of ... classification algorithms for the detection of waste areas using LANDSAT TM images It is worth of noting that the 30 meters spatial resolution of the Landsat-TM sensor makes the process of detecting waste ... the basis of the expected relationships (such as contiguity) among different land structures Methods for integrating different forms of knowledge and knowledge based methods are therefore needed...

Ngày tải lên: 16/01/2014, 16:33

... supervised classifier for identifying speculation cues and a manually compiled list of lexico-syntactic rules for identifying their scopes For the performance of the rule based system on identifying ... work, we will explore the use of statistically based methods for the creation of an optimum set of lexico-syntactic tree patterns and will evaluate the system performance on texts from different ... criteria based on exact match of both cues and their corresponding scopes4 CoNLL-2010 shared task participants applied a variety of rule -based and machine learning methods F1-scores are computed based...

Ngày tải lên: 20/02/2014, 04:20

... percentage of contaminated cultures; review question B: the name and manufacturer of the reference standard • Details of index test: software version of test • Details of comparator: type of microscopy: ... summary estimates of the diagnostic accuracy of Xpert MTB/RIF for detection of rifampicin resistance, using solid or liquid culture as a reference standard (WHO 2008) Purpose of index test: B.1 ... summary estimates of the diagnostic accuracy of Xpert MTB/RIF for detection of rifampicin resistance, using solid or liquid culture as a reference standard (WHO 2008) Purpose of index test: B.1...

Ngày tải lên: 06/03/2014, 04:20

... intrinsic structure of the silicon- based nanomaterials This is an important step toward design and control of nanostructures The Si -based nanostructures could offer great opportunities for fundamental ... tip to form either an amorphous silicon oxide sheath (for thick wire) or an amorphous silicon oxide wire (for thin wire) The formed solid SiO2 component can retard the lateral growth of the nanowires ... consists of a crystalline Si core and an amorphous outer layer Growth of Silicon- Based Nanostructures J Phys Chem B, Vol 105, No 13, 2001 2509 Figure SEM images of the nanomaterials formed in...

Ngày tải lên: 16/03/2014, 15:30

... that of the adsorbed PQ aggregates Therefore, the contribution of the LDOS of the SiNWs at the proximity of the Fermi level is presumably Fig STM filled states images of the adsorption of Å of THAP ... expected for the PQ molecule ($2 eV for an isolated molecule [15]) Since the thickness of the ONWs is of the order of several ångström, the observed LDOS is a convolution of the LDOS of the SiNWs ... [14], in the case of PQ adsorbed on Si(0 1) In summary, we presented here a novel approach to form 1D organic structures based on the adsorption of organic molecules on silicon nanowires We demonstrated...

Ngày tải lên: 16/03/2014, 15:35

... specific for apoptosis but not for necrosis (10) 1.2 DNA Damage Detection In Situ: Potentials and Pitfalls The development of techniques for in situ end-labeling (ISEL) of fragmented DNA has allowed ... useful for determining the nature of the ends of DNA from apoptotic cells 1.6 Limitations DNA fragments with 3'-OH ends can be produced in a number of situations where apoptosis is not occurring For ... iodide signal (DNA content) on the X axis and TUNEL labeling (DNA breaks) on the Y axis 3.3 Determining the Nature of the Ends of the Fragments 3.3.1 Processing of Cells and Extraction of DNA Each...

Ngày tải lên: 11/04/2014, 09:47

... Number of Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes ... Number of Number Number of Number Number of Number Results r of mice cleared mice cleared of of mice mice cleared of of mice mice cleared of of mice of monitoring mice of trypanosomes killed trypanosomes...

Ngày tải lên: 28/04/2014, 13:08

... at 290 g (1500 rpm) for minutes Cells were re-suspended in 0.5 mL of FACS buffer Ten μL of Propidium Iodide (PI) was added before acquisition for viable cell gating Total of 10,000 to 20,000 ... initiation of the validation study (data not shown) Tetramer (0.1 μg/mL) titration (2.5, 5, 10, and 20 μL) was performed and the use of 10 μL was found to be optimal Long term performance of the ... also Page of 25 (page number not for citation purposes) Journal of Translational Medicine 2008, 6:61 serve as antigen presenting cells (APC) in real time RTPCR and ELISPOT analysis Proof of principle...

Ngày tải lên: 18/06/2014, 15:20