signaling alterations in colorectal cancer

... bovine serum GEO colorectal cancer cell lines were a gift from G Tortora (Dipartimento di Endocrinologia e Oncologia Molecolare e Clinica, ` Universita di Napoli, Italy), and were maintained in ... synergistic anticancer effects in colorectal cancer cell lines We confirmed that CDO was cytotoxic in two of the cell lines studied at nanomolar concentrations Additionally, we showed that combining CDO ... that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence...

Ngày tải lên: 07/03/2014, 15:20

... NHS National Institute for Clinical Excellence Guidance on Cancer Services Improving Outcomes in Colorectal Cancers Manual Update Improving Outcomes in Colorectal Cancers Cancer service guidance ... three main tasks The first was to breathe new life into dialogue about services for managing bowel cancer by highlighting key issues and enhancing and clarifying the original text In refocusing attention ... cancer deaths in the 34 35 36 37 38 20 Bingham SA, Day NE, Luben R, Ferrari P, et al Dietary fibre in food and protection against colorectal cancer in the European Prospective Investigation into...

Ngày tải lên: 28/03/2014, 23:20

... These surprising findings suggest that TCF4 might be the main binding partner for b-catenin during development and progression of colorectal cancer whereas an enhancement of Wnt/bcatenin transcriptional ... TCF4 expression in colorectal cancer To investigate the localisation of LEF-1 and TCF4 in human colorectal cancer, we evaluated the expression of these proteins by immunostaining on tissue microarrays ... evidence for a role of beta-catenin/LEF-1 signaling pathway in induction of EMT Cell Biol Int 2002, 26:463-476 12 Li TW, Ting JH, Yokoyama NN, Bernstein A, van de Wetering M, Waterman ML: Wnt activation...

Ngày tải lên: 18/06/2014, 16:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... Tabernero J: Novel targets for anticancer treatment development in colorectal cancer Clin Colorectal Cancer 2006, 6:265-272 Voutsadakis IA: Pathogenesis of colorectal carcinoma and therapeutic implications:...

Ngày tải lên: 18/06/2014, 19:20

... in colorectal cancer CK2a protein expression was analyzed in 144 patients (104 with CRC and 40 with colorectal adenoma) Staining for CK2a was nearly negative in all of the normal Page of 11 colorectal ... expression in colorectal cancers, adenomas and adjacent normal colorectal mucosa Staining was (A) negative in normal colorectal epithelium cells, (B, C) weak to moderate in the nuclei of colorectal ... Murray T, Thun MJ: Cancer statistics, 2008 CA Cancer J Clin 2008, 58:71-96 Sung JJ, Lau JY, Goh KL, Leung WK: Increasing incidence of colorectal cancer in Asia: implications for screening Lancet Oncol...

Ngày tải lên: 18/06/2014, 19:20

... effect induced by 5-FU in the KIAA0247expressing cells could be crucial in controlling the G2/ M checkpoint and in decreasing the number of cells in G2/M At the same time, reduced levels of cyclins ... Chloroquine potentiates the anticancer effect of 5-fluorouracil on colon cancer cells BMC Cancer 2010, 10:370 35 Cunningham D, Atkin W, Lenz HJ, Lynch HT, Minsky B, Nordlinger B, Starling N: Colorectal ... Tabernero J: Novel targets for anticancer treatment development in colorectal cancer Clin Colorectal Cancer 2006, 6:265-272 Voutsadakis IA: Pathogenesis of colorectal carcinoma and therapeutic implications:...

Ngày tải lên: 20/06/2014, 03:20

... apoptosis in colorectal cancer cell lines [13,14] In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell line Methods ... activity levels in cells treated with cisplatin and oxaliplatin in combination with doxycycline (p = 0.001 and p = 0.039 respectively) These findings corresponded with the findings obtained in caspase ... Discussion Colorectal cancer constitutes the third most common cancer and the second leading cause of cancer deaths in UK About one in 20 men develops colorectal cancer in their lifetime (Cancer...

Ngày tải lên: 09/08/2014, 04:20

... suppression in colorectal cancer patients Clin Cancer Res 2010, 16:4105-4112 Ohtani H: Focus on TILs: prognostic significance of tumor infiltrating lymphocytes in human colorectal cancer Cancer Immun ... Foxp3+ cells This indicates that the Treg population was not diminished in IBD patients, a finding in direct contrast to Clarke et al We are currently investigating this further to examine the role ... effector T cell responses in tumour draining lymph nodes [27] Recent data also indicated that the presence of Foxp3+ T cells in tumour draining lymph nodes of colorectal cancer patients correlated...

Ngày tải lên: 10/08/2014, 10:21

... transmembrane glycoproteins: the extracellular domain has the ligand binding site and also contains specific sequences that are involved in dimerization, while the intracellular domain is a catalytic ... detected in many human cancers, including CRC Monoclonal antibodies that target EGFR can be effective as anticancer therapy in several ways They can block ligand binding to the receptor, aborting ... activating mutation occurs in the KRAS gene, the RAS G-protein activates the mitogen-activated protein kinase (MAPK) signaling cascade downstream of EGFR This may bypass the need for ligand binding...

Ngày tải lên: 10/08/2014, 22:20

... of deviating skipping -3 -2 -1 Relative amounts of deviating inclusion skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High or low total amounts of deviating exon ... deviating skipping 1.5 0.5 -2.5 -2 -1.5 -1 -0.5 0.5 Relative amounts of 1.5 deviating inclusion -0.5 -1 -1.5 -2 skewed TIN (sTIN): Preferential exon skipping or inclusion overall TIN (oTIN): High ... (Table S5A in Additional file 2) However, patients with either sTIN or oTIN cancers (a) Splicing Stage MSI-status sTIN oTIN (b) Stage II CRC / MSS tumors / no sTIN / no oTIN Splicing Stage MSI-status...

Ngày tải lên: 11/08/2014, 12:21

... 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... 2005-20093 Colorectal cancers usually begin as non-cancerous polyps in the inner lining of the colon or rectum4 Over many years, adenomatous polyps in the dysplasic colon may develop into pre-neoplastic ... xiii Chapter Introduction 1.1 Colon cancer 1.1.1 Colorectal carcinoma Colorectal carcinoma (CRC) is a perennial concern in public health1 According to yearly estimates from the American Cancer Society...

Ngày tải lên: 09/09/2015, 11:25

... and the role of this pathway in rapamycin sensitivity in cancer therapy; To examine the combination therapy by using PDK1 inhibitor and mTOR inhibitor in colorectal cancer The present study identifies ... mTOR signaling deregulated in human cancer Recent studies showed that silencing of targets of mTORC1 with tumor suppressive function are also involved in the aberrant of mTOR signaling during cancer ... strategy in treating rapamycin-resistant colorectal cancer 32 Chapter II: Materials and Methods 33 2.1 Cell lines and cell culture 2.1.1 Colorectal cancer cell lines The human colorectal cancer...

Ngày tải lên: 09/09/2015, 18:52

... GTBP G/T binding protein TIMP Tissue Inhibitor of Metalloproteinases DISC Death-Inducing Signaling Complex Apaf-1 Apoptotic protease-activating factor-1 IAP Inhibition of Apoptosis Proteins Smac ... factors in colorectal cancer, molecular markers may also be useful prognostic and predictive indicators in colorectal cancer as they have been shown to be in other cancers In breast cancer, for ... death-inducing signaling complex; IAPs, inhibition of apoptosis proteins; Smac/DIABLO, second mitochondria-derived activator of caspases/direct IAP-binding protein with low pI 10 Both the intrinsic...

Ngày tải lên: 14/09/2015, 08:50

... Genotyping SNP genotyping in clinical samples were carried out using the MassArray iPLEX genotyping platform (Sequenom, San Diego, CA) according to manufacturer‟s instructions SNPs, consisting of ... different amino acid or premature stop codon respectively For SNPs that are not in the proteincoding regions, they may still contribute to changes in gene splicing, transcription binding or affect ... was achieved by incubation with 3,30-diaminobenzidine (DAB) for 10 min, followed by incubation with Bond DAB enhancer For the double staining of GZMB/CD8 and CD4/FOXP3, heat-induced antigen retrieval...

Ngày tải lên: 03/10/2015, 20:57

... expressed in a wide array of human cancers, including colorectal cancer [3,4] Nevertheless, the molecular signalling pathways modulated by miRNAs, which play a role in colorectal cancer, as well as cancer ... apoptosis In addition, miR-143 expression has been shown to increase after a-mangostin exposure in human colon cancer DLD-1 cells, resulting in increased apoptosis [31] Furthermore, a-mangostin acted ... viability in a dose-dependent manner [5] We were particularly interested in investigating the effect of miR-143 on the cellular response to 5-FU 5-FU is a well-known apoptosis-inducing drug; indeed,...

Ngày tải lên: 07/03/2014, 00:20

... prognosis in colorectal cancer patients J Transl Med 2005, 3:3 Ropponen KM, Eskelinen MJ, Lipponen PK, Alhava E, Kosma VM: Prognostic value of tumour-infiltrating lymphocytes (TILs) in colorectal cancer ... Ohtani H: CD8+ T cells infiltrated within cancer cell nests as a prognostic factor in human colorectal cancer Cancer Res 1998, 58:3491-3494 Oberg A, Samii S, Stenling R, Lindmark G: Different occurrence ... by tumour-infiltrating lymphocytes in oral squamous cell carcinoma Cancer Immunol Immunother 1996, 43(1):10-8 Erratum in: Cancer Immunol Immunother 1997, 45:61 36 Echchakir H, Asselin-Paturel...

Ngày tải lên: 18/06/2014, 16:20

... both as single agent or in combination: a 2-hour intravenous infusion at 400 mg/m2 followed by weekly 1hour infusion of 250 mg/m2 In the GOIM study Cetuximab was administrated in combination with ... Figure PFS in four group of patients For corresponding lines see the key in figure Group A: +GCN/1st line; Group B: -GCN/1st line; Group C: +GCN/more lines; Group D: - GCN/more lines; (+: increased; ... antibody IMC-C225 in combination with irinotecan (CPT-11) against human colorectal xenografts Clin Cancer Res 2002, 8:994-1003 Dittmann K, Mayer C, Rodemann HP: Inhibition of radiation-induced EGFR...

Ngày tải lên: 18/06/2014, 16:20

... adenoviruses in spleen-to-liver colorectal cancer model To imitate clinical metastatic colorectal cancer, hepatic tumors were induced in mice by intrasplenic injection of HCT116 colorectal cancer cells ... for CAR binding ablation (AdTLY) This decreased transgene expression in comparison with Ad5 in all six cell lines confirming the crucial role of CAR in vitro infection in colorectal cancer cells ... Replicating viruses WT -Replicating wild type virus WT-RGD -RGD insertion in HI loop of fiber knob for avb integrin targeting [43] WT-RGDK -Fiber shaft’s KKTK domain mutated to RGDK for avb integrin...

Ngày tải lên: 18/06/2014, 16:20

... results in the treatment of CRC Since the discovery of tumor-associated antigens during the early 1990s, rapid progress has been made in identifying antigens and describing immune interactions in cancer ... cancer patients Many clinical trials have been conducted using active specific immunotherapy (ASI) in CRC, including autologous tumor cell vaccines, define-tumor protein vaccines, monoclonal antibodies ... suspected minimal residual colorectal cancer (suspected minimal residual CRC), and reviewed the objective clinical outcomes of ASI in advanced colorectal cancer (advanced CRC) during the past...

Ngày tải lên: 18/06/2014, 19:20