sepioteuthis lessoniana species complex in northwestern pacific ocean

... Medicine 57 Reagents F12 culture medium (Hangzhou Jino Biology Co., Ltd China), fetal bovine serum (FBS), ampicillin, penicillin and streptomycin (Shanghai Bio-engineering Co., Ltd China), brain ... O2, 85% N2, 10% CO2) with continuous shaking E coli were maintained in the LB liquid medium (containing 100 µg/ml ampicillin) at 37°C for 12 h with continuous shaking Preparation of Hp and E.coli ... JY, et al Vacuolating cytotoxin in Helicobacter pylori water-soluble proteins upregulates chemokine expression in human eosinophils via Ca2+ influx, mitochondrial reactive oxygen intermediates,...

Ngày tải lên: 25/10/2012, 11:18

... outbreeding depression in interpopulation crosses spanning a wide range of divergence Evolution 53:1757–1768 Einsle, U 1996 Cyclops heberti n sp and Cyclops singularis n sp., two new species within ... COPEPOD SPECIES were 197 parsimony-informative sites for COI, and consistency and retention indices were 0.64 and 0.84, respectively All substitutions in COI were synonymous, resulting in no amino ... area in this region (Fig 3) In contrast, interpopulation genetic divergences were low in Europe, with maximum divergences of only 1% in 16S rRNA and 3% in COI Morphological variants within Europe,...

Ngày tải lên: 13/02/2014, 15:20

... includes preventing and treating sexually transmitted infections, including HIV and AIDS In addition, reproductive health care can bring patients into the healthcare system, encouraging diagnosis ... of adolescent boys in the Pacific report having ever used a modern method of contraception, including condoms Studies indicate that providing SRH information early on in life can have long-term ... their education, find employment and therefore more likely to remain in poverty Teenage pregnancy is linked to an increased risk of unsafe abortion, which can lead to injury, infertility, chronic...

Ngày tải lên: 14/02/2014, 21:20

... lettuce is thinned within rows to the final spacing of 12 – 1 6in (30 – 40cm) Transplanted lettuce would be spaced at the final spacing of 12 – 1 6in (30 – 40cm) within row and 18 – 3 0in (46-76cm) ... reflect the producer’s intent in use or commercial sale of seed Symptoms appear first as pale veins (“vein clearing”), becoming a mosaic pattern in older tissue The inner leaves of infected plants may ... self-pollinating annual which produces a dense rosette of leaves early in the season, followed by flower stalk initiation whereby the central cylindrical stem elongates and indeterminate flowering...

Ngày tải lên: 21/02/2014, 21:20

... documented in this book is, in a sense, to build a bigger machine, one covering not a single bay or gulf, but an entire ocean basin It covers not one fish species of interest, but all species in the ... technological innovations of the nineteenth century Ice began to be used for preservation in the mid-1800s; steam engines were introduced in the 1860s; steel trawl lines in the 1880s; and otter trawling in ... energy used in catching groundfish and invertebrates in Iceland, and Eastern Canada Going, going… 49 The larger, predatory fish types in the North Atlantic have been decreasing since the 1950s,...

Ngày tải lên: 06/03/2014, 18:21

... including the dehydrogenase domain Specific binding of the FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-terminus For the integration into the Tic complex ... domains are degenerate 34-amino acid repeats forming anti-parallel a-helices known to be involved in an array of protein–protein interactions (generally with non-TPR proteins) [41] Both domains ... four-transmembrane domain proteins Topology-predictions indicate an Nin/Cin orientation Tic62 belongs to the extended family of SDRs and can be divided in two distinct modules The N-terminus contains the dehydrogenase...

Ngày tải lên: 07/03/2014, 03:20

... treatment To investigate whether HU induces an increase of the Hat1 protein, we used a yeast strain expressing a hemagglutinin (HA)-tagged version of Hat1 The Hat1–HA protein level did not increase ... acetylation by the yeast HAT-B complex in vivo Remarkably, the inability of the HAT-B complex to acetylate histone H4 containing the K12R substitution, both in vivo and in vitro, indicates a sequential ... detection difficult We persisted in investigating the in vivo specificity of the yeast HAT-B complex, and found that incubation of cells with hydroxyurea (HU) resulted in an increase of the histone H4...

Ngày tải lên: 07/03/2014, 05:20

... within Papua New Guinea Purchasing receipts will be considered The main impediment to increasing the use of no-take marine reserves is that there is no legal framework for implementing within ... or declining? National authority Provincial government Island council Community Traditional leaders Responses Increasing Stable Declining Do not know 40 Are fishers collecting lower-value species ... workshop), a wider range of species is now exploited than in the past  In 80 percent of the fisheries, the proportion of low-value species in catches is increasing Stakeholder involvement and communication...

Ngày tải lên: 14/03/2014, 10:12

... Production in the Pacific 14 Shipping seaweed in the Pacific Internal shipping Export shipping 15 15 16 Marketing in the Pacific Farm-gate prices International sales Cooperation in marketing 17 18 ... industry in the Pacific islands ACIAR Working Paper No 61, 55 pp The seaweed industry in the Pacific islands the costs below are based on picking up a full container within Suva and delivering ... use in the floating-raft method of farming 42 McHugh, D.J 2006 The seaweed industry in the Pacific islands ACIAR Working Paper No 61, 55 pp The seaweed industry in the Pacific islands On Fanning...

Ngày tải lên: 14/03/2014, 10:13

... found only in the species C eugametos, and possesses characteristics of the well-known self-splicing protein elements called inteins [27–29] Inteins are autonomously folding protein domains capable ... (1992) Protein splicing removes intervening sequences in an archaea DNA polymerase Nucl Acids Res 20, 6153– 6157 29 Paulus H (2000) Protein splicing and related forms of protein autoprocessing Annu ... ClpP1L in denaturing gel Both of them originated from the last 90 C-terminal residues of the protein, one of them from the very C terminus In the MS analysis, no peptides were found corresponding...

Ngày tải lên: 16/03/2014, 23:20

... as well as furthering the plot It is because of Emile's acceptance of the mission that helps Nellie realize that her prejudices are stupid and that she's giving away everything that is important ... everything that is important to her because of someone else's preconceived notions of people By examining some of the social problems of the time (and even now), Act 2, scene 3, proves to be one of...

Ngày tải lên: 21/03/2014, 22:49

... has finished singing, Joe sees the opportunity to take advantage of the situation He asks Emile if he would reconsider going on the spy mission with him, now that he has nothing to risk losing ... Emile agrees and they set off to tell Captain Brackett that they will be doing the mission after all Act 2, scene 3, is a very important scene in South Pacific because it tackles the problem of ... prejudices are stupid and that she's giving away everything that is important to her because of someone else's preconceived notions of people By examining some of the social problems of the time...

Ngày tải lên: 21/03/2014, 22:49

... impression is supported in the indifferent performance of key social indicators in Papua New Guinea However, since the early post-independence survey of policy making in Papua New Guinea edited by John ... wish lists of projects but in sharing information, participating in planning and assuming some responsibility for accountability Seeking good practice in Papua New Guinea and how to replicate ... effectiveness in public service training: the case of the Administrative College of Papua New Guinea Administration for Development 25: 1–59 24 Explaining Public Sector Reform Failure: Papua New Guinea...

Ngày tải lên: 22/03/2014, 21:20

... protein complex In the present study, the data indicate that the phytyl chain in Chl is of central importance Bleaching of the tetrayrrol moiety in Chl maintained the Cyt b6f complex in a functional ... mixing of the two samples, they were separated by BN- ⁄ SDS-PAGE and the gel was read out in a Typhoon imager 9400 Proteins originating from the etioplast are shown in red; proteins originating ... in green; proteins present in both membranes in equal amounts are shown in yellow Complex subunits are labelled according to Granvogl et al [23] The dimeric Cyt b6f complex is boxed (white lines)...

Ngày tải lên: 23/03/2014, 07:20

... protein, containing both the PTB domain and two of the N-terminal proline-rich domains This interaction was only slightly inhibited by phosphotyrosine To test if the Shb PTB domain could be involved ... binding-site D-DX-Y Two such motifs were found in Vav and synthetic peptides corresponding to them were made These peptides were used in binding experiments with a GST fusion protein containing ... phosphotyrosine motif in Shb, and the proline rich sequences of Shb interact with some other adaptor, which also has the ability to bind SLP-76 (Fig 8) After investigating possible SLP-76-interacting...

Ngày tải lên: 24/03/2014, 04:21

... E contains sites for interaction with RhlB, enolase and PNPase In addition, we have identified regions with sequence homology to ‘Segment A’ and the RNA-binding domain in E coli A remaining region ... three times using NaCl ⁄ Pi and the target protein was eluted by incubating the beads in 1· SDS buffer for 15 at 55 °C or boiling the beads in 1· SDS gel loading buffer for 10 Following SDS ⁄ PAGE ... degradosome protein complex, we used two-dimensional BN-SDS ⁄ PAGE In this technique, protein complexes can be separated into subunits in an SDS ⁄ PAGE second dimension, following first dimension...

Ngày tải lên: 29/03/2014, 21:20

... cisplatin, indicating that all three proteins may operate as a functional unit in vivo, playing a role in the cisplatin response [9] Cisplatin interferes with DNA function by causing intrastrand ... Vazquez-Martin et al Cisplatin-induced DNA damage response that participate in these pathways are not well delineated Several studies have implicated complexes of protein serine ⁄ threonine phosphatase ... causing intrastrand and interstrand crosslinking of nucleotide bases and, in replicating cells, DNA damage usually induces an intra-S-phase cell cycle arrest Accordingly, we show in this article that...

Ngày tải lên: 30/03/2014, 04:20

... constructed An alanine residue was inserted after Phe18 in order to disrupt a helix–helix packing interface involving the aminoacid residues on both sides of the insertion [40] In the other two ... cells were incubated in the absence or in the presence of CuCl2 (A) Digitonin extracts obtained with the indicated digitonin-to-protein ratios were submitted to BN/PAGE The gel was incubated with ... a bis-maleimide reagent and involving a cysteine residue introduced into the C-terminal part of subunit i located in the intermembrane space has been reported both in detergent extracts which...

Ngày tải lên: 31/03/2014, 01:20

... including Tanzania, injuries in general and splenic injury in particular are increasing due to increase in urbanization, motorization, civil violence, wars and criminal activities [5] In Bugando Medical ... firearms, arrows and spears the incidence of penetrating splenic injuries has been increased in civil society [9] In the past century, the management of splenic injury has continued to evolve from a focus ... Routine Investigations included hematological (hemoglobin, Total Leukocyte count, blood grouping & platelet count), biochemical (serum creatinine & serum electrolytes), serology (HIV testing)...

Ngày tải lên: 21/06/2014, 19:20

... TNHH TM-DV PACIFIC OCEAN VIỆT NAM TRONG ĐIỀU KIỆN ÁP DỤNG PHẦN MỀM KẾ TOÁN 22 2.1- Đặc điểm tổ chức hoạt động sản xuất kinh doanh tổ chức công tác kế toán tạCông ty TNHH TM-DV Pacific Ocean Việt ... TM-DV Pacific Ocean Việt Nam 22 Sơ đồ Kế toán kho liên quan đến ngành nghề kinh doanh công ty??? 27 2.1.4: Kết hoạt động kinh doanh năm gần Công ty TNHH TM-DV Pacific ... TNHH TM-DV PACIFIC OCEAN VIỆT NAM TRONG ĐIỀU KIỆN ÁP DỤNG PHẦN MỀM KẾ TOÁN 2.1- Đặc điểm tổ chức hoạt động sản xuất kinh doanh tổ chức công tác kế toán tạCông ty TNHH TM-DV Pacific Ocean Việt...

Ngày tải lên: 25/07/2014, 09:20