separation divorce and remarriage as family processes

Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx

Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx

... glutathione, mgÆmL)1 reduced RNase A and 0.25 mgÆmL)1 recombinant GmPDIM and was incubated at 25 °C An aliquot (16 lL) of the reaction mixture was removed and RNase A activity was measured spectrophotometrically ... of the resulting peptides were mapped as described previously [20] Oxidative refolding assay with reduced RNase A PDI activity was assayed by measuring RNase activity produced through the regeneration ... thiol-oxidoreductase P5 [7] The recombinant form of GmPDIM was active as it could refold RNase A that had been reduced and denatured The specific activity of GmPDIM was very similar to those of GmPDIS-1 and...

Ngày tải lên: 30/03/2014, 04:20

12 348 0
Separation, extraction and concentration processes in the food, beverage and nutraceutical industrie

Separation, extraction and concentration processes in the food, beverage and nutraceutical industrie

... Fig 1.10 SLG phase lines for binary systems of vanillin (V) and o-vanillin (o-V) with dense gases By denoting the gas as component and the non-volatile compound as component 2, and assuming that ... solubility of a solute increases with increasing density and decreases with decreasing density A decrease in density can be obtained by decreasing pressure and/ or increasing temperature In many ... areas of homogeneous liquid, gas, SCF and two-phase liquidgas region (LG) The dotted line separates the area of SCF from the gas and liquid state area and presents no phase border The part of the...

Ngày tải lên: 20/09/2015, 01:57

1.4K 3.1K 0
Functions and variables as symbols

Functions and variables as symbols

... Review: Hash tables • Hash table (or hash map): array of linked lists for storing and accessing data efficiently • Each element associated with a key (can be an integer, string, or other type) • Hash ... elsewhere (like the C standard library) and makes the code executable Athena is MIT's UNIX-based computing environment OCW does not provide access to it Functions and variables as symbols • Let’s ... executable • addresses for lines of code and static and global variables not yet assigned • need to perform link step on hello.o (using gcc or ld) to assign memory to each symbol • linking resolves...

Ngày tải lên: 25/04/2013, 08:07

46 291 0
Design and Controlof Workflow Processes

Design and Controlof Workflow Processes

... previous classifications, the above levels of decision making can be distinguished within primary, secondary, and tertiary processes, as well as within mass-customization and mass-production processes ... Management as put forth by Leymann and Altenhuber (1994) They distinguish two fundamental aspects, namely the build time aspect and the run time aspect of managing business processes The build time aspect ... this type of technology has not caught on as was expected Technological as well as change management issues are seen as major reasons for this Reijers et al (1999), Reijers and Goverde (1999b), Grinter...

Ngày tải lên: 21/07/2013, 09:42

322 382 0
Báo cáo khoa học: Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase/helicase activities of hepatitis C and related viruses ppt

Báo cáo khoa học: Halogenated benzimidazoles and benzotriazoles as inhibitors of the NTPase/helicase activities of hepatitis C and related viruses ppt

... repair, and a member of the same helicase II superfamily, in contrast to PcrA helicase, which belongs to the helicase I superfamily [46] Both structures, NTPase/helicase complexed with dU8, and UvrB ... TBBT, and a number of related benzotriazoles and benzimidazoles, at the NTPase/helicase sites of HCV and the related viruses Japanese encephalitis virus (JEV) and WNV, as well as the human NTPase/ ... lM, 235 lM and 4.2 lM for WNV, HCV, JEV and Suv3(D1–159) NTPase/helicases, respectively, and 4.7 pmol of DNA substrate (concentration of nucleotide base) as described in Materials and methods...

Ngày tải lên: 08/03/2014, 02:20

9 659 0
catalyst separation, recovery and recycling

catalyst separation, recovery and recycling

... products, and it offers the opportunity to use a very much wider array of ligands and separation solvents than prior-art phase separation processes The phase distribution characteristics of the ligand ... becomes water-soluble and can be separated from product by phase separation Product and organic byproducts are in the organic phase; rhodium and ligand are in the polar phase If ligand scrambling occurs, ... CATALYST SEPARATION, RECOVERY P R R AND RECYCLING Chemistry and Process Design Edited by DAVID J COLE-HAMILTON EaStCHEM, School of Chemistry, University of St Andrews, St Andrews, Fife, Scotland and...

Ngày tải lên: 11/03/2014, 22:58

255 275 2
optimisation of artemia biomass production in salt ponds in vietnam and use as feed ingredient in local aquaculture

optimisation of artemia biomass production in salt ponds in vietnam and use as feed ingredient in local aquaculture

... generated from Asian countries, with China and Vietnam ranking as the first and third global producers Among the top ten Asian producers, Vietnam experienced the fastest growth between 1985 and 2005, ... one thousand hectares and it was reported that the total aquaculture area and productivity have increased 2.1 and 2.5 times, respectively, compared to that in 1996 A similar tendency was also ... farming activities and has a large potential for increased aquaculture production (MoFI, 2005) The culture systems include pond, fence and cage culture of catfish (Pangasius) as well as several indigenous...

Ngày tải lên: 13/03/2014, 18:58

296 663 0
Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc

Báo cáo khoa học: PCSK9 is phosphorylated by a Golgi casein kinase-like kinase ex vivo and circulates as a phosphoprotein in humans doc

... that has no significant effect on plasma cholesterol levels and the R46L variation, a naturally occurring variant associated with hypocholesterolemia [31] and reduced plasma PCSK9 [22] As shown ... Rivier JE & Fischer WH (1994) Monitoring protein kinase and phosphatase reactions with matrix-assisted laser desorption ⁄ ionization mass spectrometry and capillary zone electrophoresis: comparison ... vivo in human plasma; (b) in the case of the propeptide, phosphorylation is decreased in the naturally occurring ‘loss-of-function’ R46L and A53V PCSK9 variants; and (c) decreasing the level...

Ngày tải lên: 16/03/2014, 06:20

14 454 0
Midlife and Older Women Family Life, Work and Health in Jamaica pot

Midlife and Older Women Family Life, Work and Health in Jamaica pot

... day, Hope Pastures was a very quiet place Husbands and wives and young adults were away at work, and children and grandchildren were at school Maids and gardeners were within the yards and homes ... were seen mainly as mothers, grandmothers and caregivers,5 and although these designations were associated with various tasks, these tasks were not seen as work by the relatives and other respondents ... middle classes, especially the middle and upper middle class appeared to be less stressful and more predictable and ordered than the lives of lower-middle- and working-class women We reasoned that...

Ngày tải lên: 16/03/2014, 12:20

184 380 0
Investigation of au and in as solvents for the growth of silicon nanowires on si(1 1 1)

Investigation of au and in as solvents for the growth of silicon nanowires on si(1 1 1)

... different behavior of gold and indium on Si(1 1) has been described and analyzed An explanation based on differences of solubilities of surface atoms in gold and indium and on the different surface ... bare and oxidized substrate as well as on the surface tension of the liquid metal alloys has been presented Silicon nanowires have been grown via VLS mechanism with the use of gold and indium as ... [13] As the surface tension of most liquids decreases in a nearly linear fashion with increasing temperature [14], there is a wide difference between the surface tension of the Au–Si alloy and...

Ngày tải lên: 16/03/2014, 15:17

6 566 0
Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

Báo cáo khoa học: Molecular characterization of recombinant mouse adenosine kinase and evaluation as a target for protein phosphorylation potx

... 271) PKA was purified from bovine heart as previously described [11] PKG and cGMP were purchased from Promega; MAPK, CaMKII, and calmodulin from Upstate; and CK1, CK2, and Cdk1 from New England Biolabs ... inhibitor-1 and DARPP-32 were generated as previously described [13,14] Recombinant tyrosine hydroxylase was kindly provided by P Fitzpatrick and C Daubner, Texas A&M University Purified histone H1 and ... Activities and some properties of 5¢-nucleotidase, adenosine kinase and adenosine deaminase in tissues of vertebrates and invertebrates in relation to the control of the concentration and the physiological...

Ngày tải lên: 16/03/2014, 18:20

9 497 0
Báo cáo khoa học: Coenzyme A affects firefly luciferase luminescence because it acts as a substrate and not as an allosteric effector pot

Báo cáo khoa học: Coenzyme A affects firefly luciferase luminescence because it acts as a substrate and not as an allosteric effector pot

... (which was 4.9 mm and mLặmin)1, for the case of CoA, and mm and 1.7 mLặ min)1, for the case of dephospho-CoA), the aliquots were analysed as described above In parallel, luminometer based assays ... luciferase and acyl-CoA synthetases, our achievement is not as strange as it seems to be and supports the theory that nowadays rey luciferase evolved from an ancestral acyl-CoA synthetase [1] ... the presence and in the absence of PPase (Fig 8) As expected, the decay was more pronounced when higher concentrations of ATP and LH2 were used and even more pronounced when PPase was simultaneously...

Ngày tải lên: 16/03/2014, 23:20

11 475 0
Báo cáo Y học: Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes pot

Báo cáo Y học: Synthesis of phosphoenol pyruvate (PEP) analogues and evaluation as inhibitors of PEP-utilizing enzymes pot

... 7.4, 2.2) Scheme PEP carboxylase, and enolase Compounds 2a–e, 2g–i and 3c are new, and for that reason their synthesis and characterization is also reported, as well as an improved method for the ... UÆmL)1 of pyruvate kinase and mM MgCl2 or 0.05 UÆmL)1 of pyruvate kinase and mM MnCl2 PEP carboxylase activity was determined in a coupled assay with malate dehydrogenase, as described previously ... pyruvate kinase/L-lactate dehydrogenase assay Obviously, this methodology was not applied with compounds 1b–e (also 1f and 2f when Mn2+ was present) as they are good inhibitors of pyruvate kinase In...

Ngày tải lên: 18/03/2014, 01:20

11 643 0
composite of tio2 nanowires and nafion as humidity sensor material

composite of tio2 nanowires and nafion as humidity sensor material

... was >99% (Sigma–Aldrich Co., Inc., USA) The Nafion® solution was obtained from Aldrich (USA) and the concentration was wt.% in a mixture of lower aliphatic alcohols and water The TEOS (98%) as ... the flow rates of saturated air and dry air, and ‘f’ was the coefficient of this system which was dependent on temperature and flow rate The homemade simple apparatus has been developed for producing ... stability and optical transparency of the composite films The IR spectrum of the hydrated titanium dioxide [h-TiO2 ] shows a large broad band between 3250 and 3490 cm−1 and narrow bands at 1641 and...

Ngày tải lên: 19/03/2014, 16:47

7 562 0
Báo cáo khoa học: "Parsing and Generation as Datalog Queries" doc

Báo cáo khoa học: "Parsing and Generation as Datalog Queries" doc

... (e→t)→(e→t)→t, N → e→t}) A rule that has A on the left-hand side and B1 , , Bn as right-hand side nonterminals has its left-hand side annotated with a well-formed λ-term M that has type σ(A) under the type ... Montague semantics for English and such “linear” grammar formalisms as (multi-component) tree-adjoining grammars (both as string grammars and as tree grammars) and multiple context-free grammars ... like free variables In the case of the second-to-last rule, the principal typing is We then obtain the corresponding database (3) and query (4) from the antecedent and succedent of this judgment,...

Ngày tải lên: 23/03/2014, 18:20

8 305 1
Báo cáo khoa học: RNA helicase A interacts with nuclear factor jB p65 and functions as a transcriptional coactivator pot

Báo cáo khoa học: RNA helicase A interacts with nuclear factor jB p65 and functions as a transcriptional coactivator pot

... the cells were harvested, and the extracts were prepared for luciferase assay Luciferase activity was measured by the Luciferase Assay System (Promega, Madison, WI) as described previously [26] ... expression plasmid Total DNA was kept at 0.5 lg with pUC19 plasmid Cells were harvested 48 h after transfection, and luciferase activity was measured Extents of fold activation of luciferase gene ... coli strain DH5a and purified The in vitro protein–protein interaction assay (Ôpull-downÕ assay) was carried out as described previously [23,24,26] The p65 protein was synthesized and labeled with...

Ngày tải lên: 30/03/2014, 15:20

11 485 0
integrated design and simulation of chemical processes

integrated design and simulation of chemical processes

... expressed as the mass of waste (not converted material) per unit of output Waste is calculated by subtracting the mass of products and saleable subproducts from the mass of raw materials Water and ... Data and requirements 7.4 Input/Output analysis 7.5 Reactor design and recycle structure 7.6 General structure of the separation system 7.7 Vapour recovery and Gas separation systems 7.8 Liquid separation ... concept has been extended to other systemic issues, as process water saving and hydrogen management in refineries Residue Curves Map The feasibility of separations of non-ideal mixtures, as well as...

Ngày tải lên: 02/04/2014, 15:49

715 1K 0

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