senescence of dividing somatic cells

... site 457 bp upstream of the start of the element that encompasses part of intron 21 and all of exon 22 of RB1 (Figure 3a) This position is over 161 kb downstream of the start of RB1 Realtime RT-PCR ... expression of the L1 element to that of the surrounding CD38 gene We found that, while the abundance of the CD38 transcript is several orders of magnitude higher, the pattern of expression of the ... Description of data collection method and overview of results Description of data collection method and overview of results (a) Diagram of expression tag capture L1 elements are often naturally...

Ngày tải lên: 09/08/2014, 20:20

... provide a profile of B cells that respond to antigen at a given time point In an earlier investigation, Ravirajan and coworkers [7,8] demonstrated the presence of autoantibody-producing B cells in ... 0.5 cm3 portion of spleen tissue GC A can be seen in Fig 1, and has two distinct areas of staining for FDC and one large area of B cells encompassing both FDC regions Very few T cells could be ... Number of mutations The distribution of mutations between VH gene sequences in germinal centre and B-cell clusters Somatic mutations in germinal centre VH genes The distribution of the frequency of...

Ngày tải lên: 09/08/2014, 01:21

... lactation mean of somatic cells (in their natural logarithmic form) variance explained by the FYS factor was calculated by dividing the variance component of the FYS factor by the sum of the residual ... scrupulous control of sanitation of housing and equipment is necessary before any attempt of selection on somatic cells However, while taking active steps to improve the level of management, farmers ... ) LSCC LILCY = lactation mean of individual laboratory cheese yield LSCC = lactation mean of somatic cells (in their natural logarithmic form) Standard errors of genetic correlations ranged...

Ngày tải lên: 14/08/2014, 13:21

... adaptation of a lentivirus system for gene delivery, generation of cells to use as a high-throughput screening-system for analysis of virally-infected or transfected somatic cells, providing proof -of- principle ... efficacy of such treatments, this important proof -of- principle establishes the utility of transplanted iDA cells to restore function in at least one animal model of human disease or injury All of these ... Direct Reprogramming of Somatic Cells: Establishment of a Novel System for Photoreceptor Derivation Major Professor: Jason S Meyer Photoreceptors are a class of sensory neuronal cells that are deleteriously...

Ngày tải lên: 24/08/2014, 12:37

... the influence of the operating parameters on fuel cell performance to investigate the in situ total displacement and degree of the deformation of the polymer membrane of PEM fuel cells The model ... decreases with increasing of the cell operating pressure This is because of the exchange current density of the oxygen reduction reaction increases with increasing of the cell operating pressure ... simulation of the processes under realistic conditions is essential to the development and optimization of fuel cells, improve long-term performance and lifetime, the introduction of cheaper...

Ngày tải lên: 05/09/2013, 14:58

... signi®cance of this similarity is currently being addressed Demonstration of the cytosolic location of Mls1p in wild-type cells grown on ethanol completes the picture of the extra-peroxisomal location of ... (Fig 2) Lysates of homogenized wild-type cells were spun to yield an organellar pellet consisting of mitochondria and peroxisomes, and a cytosolic supernatant Equal fractions of each of the protein ... mutant cells often grow to some extent on these plates but transparent zones in the opaque medium around regions of cell growth indicate utilization of the fatty acid substrate [25] Application of...

Ngày tải lên: 22/02/2014, 04:20

... legend of Figure (a) and (b): numbers of cells at 144 h of culture; (c): proliferation rates of GC from small follicles at 48 h of culture; (d): pyknotic rates of GC from large follicles at 48 h of ... (percentage of labeled cells) and the pyknotic index (percentage of pyknotic cells) after 48 h of culture This has been shown to allow the be the optimal culture time for the study of the effects of various ... The percentage of the different morphological states of GC (spread cells, spindle-shaped cells, round cells) was established by counting 1000 to 1500 cells per culture well Page of 17 (page number...

Ngày tải lên: 05/03/2014, 17:20

... immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of  50 and  70 kDa, respectively ... indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform that occurred exclusively in mature dendritic cells Characterization of post-translational ... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were...

Ngày tải lên: 08/03/2014, 02:20

... released from dead cells, due to the loss of cell membrane integrity observed in both necrotic and apoptotic cells The long-term exposure of RIN-m5F cells to STZ at concentration of > 10 mm induced ... indicated that h of exposure of RIN-m5F cells to STZ induced a rapid and significant elevation in the caspase-3 activity within h, which preceded the loss of cell viability The activation of caspase-3 ... decrease in cell viability, the co-exposure of RIN-m5F cells with some neuropeptides at concentrations of 10)7 m attenuated the cytotoxicity of STZ (Table 1) Of all glucagon-secretin-family peptides...

Ngày tải lên: 16/03/2014, 04:20

... Mexico School of Medicine, MSC07 4025, University of New Mexico, Albuquerque, NM 87131, USA 3Department of Pathology, University of New Mexico School of Medicine, MSC08 46401 University of New Mexico, ... labeling of attached cells, cells were cultured on acid-washed glass coverslips Quantification of Her2 site density was performed as previously described by using approximately 105 cells and ... http://breast-cancer-research.com/content/13/5/R108 Cells only Cells only Isotype Page of 13 Her2 Isotype Her2 Isotype Cells only Her2 A B Figure Characterization of Her2 expression on breast cancer cell...

Ngày tải lên: 22/03/2014, 17:20

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... establishment of embryonic stem (ES) cells, which maintain the ability to form all types of cells in the body, and can differentiate into a variety of cell types in vitro [8] The use of ES cells in ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...

Ngày tải lên: 23/03/2014, 07:20

... Transfection of HL-60 and SL2 cells HL-60 cells were transfected using the DEAE-dextran procedure as previously described [4] Briefly, cells (2 · 107) were collected by centrifugation, resuspended in mL of ... PMA-treated HL-60 cells Fig Transient expression analysis of the three GC boxes in the proximal region of the human DNase II promoter HL-60 cells were transfected with wild-type or GC mutants of pDNaseII()249/+72)Luc, ... PMA-treated cells (Fig 4A, lane 3) The intensity of these complexes was markedly decreased in the presence of a 10- or 50-fold molar excess of unlabeled probe I (lanes and 5), but not in the presence of...

Ngày tải lên: 23/03/2014, 17:21

... sub serve the role of such agents Recently, the physiological importance of NFAT in cells other than those of the immune system has been uncovered The widespread distribution of NFAT mRNA and/or ... Rao, A & Redondo, J.M (1997) Blockade of T cell activation by dithiocarbamates involves novel mechanisms of inhibition of nuclear factor of activated T cells Mol Cell Biol 17, 6437–6447 45 Felli, ... represses the induction of FasL expression (A) The effects of all-trans-RA on FasL transcription were examined using RT-PCR Jurkat cells were incubated with the indicated concentrations of all-trans-RA...

Ngày tải lên: 24/03/2014, 03:21

... proliferation rate of LNCaP cells First, we studied whether overexpression or downregulation of TPD52 influences the proliferation rate of LNCaP cells To determine the effect of TPD52 expression ... downregulation of TPD52 in LNCaP cells MTT assays showed a significantly increased proliferation of the PCA cell line LNCaP after transient overexpression of EGFPTPD52 (Fig 3A) The proliferation of these cells ... LNCaP cells (Fig 4G) In 30% of the TPD52 knockdown cells, a significant decrease of Dwm (P £ 0.0013) could be observed, whereas nontransfected or mock transfected cells were less than 10% of the...

Ngày tải lên: 30/03/2014, 02:20

... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... screening procedure is use of FCS samples for the production of MEDII and formation of EPL cells in adherent culture The methods for production of MEDII and formation of EPL cells are given elsewhere ... Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus 30 Isolation, Characterization, and Differentiation of Human Embryonic Stem Cells HIROFUMI SUEMORI NORIO NAKATSUJI...

Ngày tải lên: 11/04/2014, 00:32

... Embryos and Somatic Cells maximum mass of 124x106 Da.1 Each NPC is composed of about 40 distinct proteins (nucleoporins or Nups), each of which is present in copies or multiples of copies Identified ... their behavior is rather static However, both in non -dividing and dividing cells there are aspects of NE function that require dynamic exchange of its proteins Before we review these, it is useful ... Dynamics in Embryos and Somatic Cells Figure Schematic view of the organization of the interphase nuclear envelope (Left) Nuclear membranes can be seen clearly as a subcompartment of the ER studded...

Ngày tải lên: 11/04/2014, 10:00

... of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric assay of soluble class I MHC-peptide com- Page of ... coculture of the HCC cells with healthy donor's T cells stimulated by unirradiated DCs/allo-HCC, the majority of the HCC cells were detached (Figure 3A, middle panel) Almost all of the HCC cells ... Induction of HCC cells- specific CTL by DCs/allo-HCC To determine whether HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from...

Ngày tải lên: 18/06/2014, 15:20

... 18th hour of incubation of monolayer- and 3D-spheroid-cultured CT26 cells For both culture conditions, the concentration of VEGF was expressed as a function of the total number Page of 12 (page ... antibody completely abrogated the adhesion of 3D-spheroid-cultured CT26 cells, but not of monolayer-cultured cells, to HSE cells (Figure 2D) Moreover, the majority of smallest avascular CT26 hepatic ... hepatic colonization ability of CT26 cancer cells Nineteen days after subcutaneous injection of monolayerand 3D-cultured CT26 cells, the number of CD31-expressing cells was determined in developed...

Ngày tải lên: 18/06/2014, 15:20

... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... 7042014 of the National Science Foundation of Beijing, China, the 50573044 of the National Natural Science Foundation of China and the 2005CB623905 of the National Basic Research Program of China ... atrophy of upper lip was evident Electromyography Electromyography is a measurement of neuromuscular function The prolongation of time-latency, increase of current threshold and decrease of voltage...

Ngày tải lên: 18/06/2014, 15:20

... cells) were able to generate cells capable of lysing SCC-4 cells The cytotoxicity was assessed after incubating 10,000 SCC-4 cells (PKH26 prelabelled) with 100,000 PBMCs and incubated for h Cells ... Phenotypic profiles of DC precursor CD14+ monocytes A Phenotypic profiles of DC precursor CD14+ monocytes Illustrating the absence of DC markers on this monocyte population B DC phenotypic profiles: ... or is cryptic on the surface of tumour cells and that immunization of cancer patients with hTERT p540 leads to the production of T cells that not recognize tumour cells in vivo based on this epitope...

Ngày tải lên: 18/06/2014, 15:20