selective chemical deuteration of aromatic amino acids a retrospective
Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions
... heart, testes Liver Spleen, thymus 22 MDR CF/MDR ALD OABP GCN20 White ABCA8 ABCA9 ABCA10 ABCA12 ABCA13 ABCB1 ABCB2 ABCB3 ABCB4 ABCB5 ABCB6 ABCB7 ABCB8 ABCB9 ABCB10 ABCB11 ABCC1 ABCC2 ABCC3 ABCC4 ... ABCC3 ABCC4 ABCC5 ABCC6 ABCC7 ABCC8 ABCC9 ABCC10 ABCC11 ABCC12 ABCD1 ABCD2 ABCD3 ABCD4 ABCE1 ABCF1 ABCF2 ABCF3 ABCG1 ABCG2 ABCG4 ABCG5 ABCG8 PGY1, MDR TAP1 TAP2 PGY3 MTABC3 ABC7 MABC1 MTABC2 SPGP ... that mutation of individual aromatic, polar, and charged amino acids can affect the recognition and transport of one or several substrates in different ways Ito et al had demonstrated that a...
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Báo cáo y học: " Albumin dialysis improves hepatic encephalopathy and decreases circulating phenolic aromatic amino acids in patients with alcoholic hepatitis and severe liver failure" doc
... Albumin (g/L) Haemoglobin (g/L) Leucocytes Platelets (cell/×109) (cell/×109) Total amino acids (μM/L) AA = amino acids; ALT = alanine aminotransferase AP = alkaline phosphatase; AST = aspartate ... of the abnormal amino acid profile, basically by decreasing phenolic aromatic amino acids These results may explain at least in part the hopeful effects of albumin dialysis on hepatic encephalopathy ... Circulating levels of total, branched and aromatic amino acids Results are shown in patients with alcoholic hepatitis (AH) and pruritus (control) Circulating levels of total, branched and aromatic...
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Báo cáo y học: "A new parameter using serum lactate dehydrogenase and alanine aminotransferase level is useful for predicting the prognosis of patients at an early stage of acute liver injury: A retrospective study" potx
... Shimada H: Usefulness of artificial liver support for pretransplant patients with fulminant hepatic failure Transplant Proc 2004, 36:2355-2356 Mita A, Hashikura Y, Tagawa Y, Nakayama J, Kawakubo ... enzyme released in liver injury, as are aspartate aminotransferase and alanine aminotransferase (ALT) It is common to regard monitoring serum LDH as of little value because it is produced in various ... normal range of our assay system, the median of the serum LDH was calculated as 174 U/L in this study Statistical analysis Differences in clinical backgrounds and laboratory data between conservative...
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Báo cáo y học: "De-escalation as part of a global strategy of empiric antibiotherapy management. A retrospective study in a medico-surgical intensive care unit" pdf
... de-escalation may answer this question Conclusions As part of a global management of empiric antibiotherapy in an ICU, de-escalation might be safe and feasible in a large proportion of patients and ... the statistical analysis JC, RJ and CA gathered and analyzed the data JM, JC, SM and CA drafted the manuscript All authors read and approved the final manuscript Competing interests The authors ... study was to assess the application of a de-escalation strategy on empirical antibiotics management We particularly analyzed the clinical impact of this attitude in terms of re-escalation, recurrent...
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Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc
... Braunstein, A. E (1947) Labilization of a- hydrogen of amino acids under the action of aminoferase Biokhimia 12, 556–568 (in Russian) Ó FEBS 2004 Esaki, N., Nakayuma, T., Sawada, S., Tanaka, H & Soda, K ... The anchoring of a- carboxylate and a -amino group in the external aldimine defines automatically the positions of the a- proton and the side chain of any bound amino acid The lability of the a- proton ... the Lys257 amino group and a water molecule providing a favorable mutual orientation of the amino group, the water, and the a- proton of the external aldimine The formation of a symmetrical six-membered...
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Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
... (Mannheim, Germany) Ethanol (> 99%) was obtained from Panreac (Barcelona, Spain) Urea was a product of Acros (Pittsburgh, PA, USA) The QuickchangeTM kit containing Pfu DNA polymerase, 10 · reaction ... Luria–Bertani broth and isopropyl thio-b-d-galactoside were purchased from USB (Cleveland, OH) Salmon testes DNA and some analytical grade chemicals such as EDTA, Tris ⁄ HCl, CaCl2, NaCl and ... conformation of denatured proteins Here we show that a specific single mutation or removal of a specific fragment can cause large changes in the native state of SNase Fig Steady-state fluorescent spectra of...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... GCCGGGATCCATGGGCAAGGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCAAGGCAGCATCTAAGGCAGCAGCAGACAAGCCTGTAGCC AATTAACCCTCACTAAAGGG GCCGGGATCCTAGGGCGAATTGGGTACC Ó FEBS 2004 Analysis of the N-myristoylation ... ATATGGATCCATGGGCGCGGCAGCAGCGGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC GCCGGGATCCATGGGCGCAGCAGCANNKGCAGCAGCAGCAGAC ATATGGATCCATGGGCNNKGCAGCAGCGGCAGCAGCAGCAGAC GCCGCTCGAGCCTGTAGCCCATGTT AATTCTCGAGTGCTGCTGCTGCCGATGCTGC ... AATTCTCGAGTGCTGCTGCTGCCGATGCTGC AATTCTCGAGTGCTGCTGCTGCCGTTGCTGC AATTCTCGAGTGCTGCTGCTGCGAATGCTGC GCCGGGATCCATGGGCAAAACGCTGAGCAAAGAGGACAAGCTCGAG GCCGGGATCCATGGGCAAGCAGAATAGCGCACTGCGGCCAGACAAG GCCGGGATCCATGGGCAAGGCAGCATCTGCAGCAGCAGCAGACAAGCCTGTAGCC...
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Báo cáo khoa học: Substrate specificity of the human UDP-glucuronosyltransferase UGT2B4 and UGT2B7 Identification of a critical aromatic amino acid residue at position 33 doc
... CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG CTGGTGTGGGCAGCAGAACTCAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGAGTTCTGCTGCCCACACCAG CTGGTGTGGGCAGCAGAATACAGCCATTGGATGAATATAAAG CTTTATATTCATCCAATGGCTGTATTCTGCTGCCCACACCAG ... Sense Antisense Sense Antisense Sense Antisense Sense Antisense CTGGTGTGGCCCACAGAACTCAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGAGTTCTGTGGGCCACACCAG CTGGTGTGGCCCACAGAATACAGCCACTGGATGAATATAAAG CTTTATATTCATCCAGTGGCTGTATTCTGTGGGCCACACCAG ... LumiGLOTM was from Cell Signaling (Beverly, MA, USA), and AdvantageÒ polymerase mix was from Clontech (Palo Alto, CA, USA) All other reagents were of the best quality and commercially available Expression...
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Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc
... CGGGCAAATGACAGCAGCTGTA-3¢, upstream; P10: 5¢-AAACCCGGGCGCAGTGTAGCTGAAG-3¢, upstream; P11: 5¢-CCCCTCGAGCCCTTAAATTGGTTGTTGTA-3¢, downstream; P12: 5¢-ATACCCGGGATGACTGCCACTA CTCAACAATTAAAACGT-3¢, upstream; ... upstream; P2: 5¢-GGGCTCGAGCGGCAATTAAAGTGG GAAT-3¢, downstream; P3: 5¢-ATACCCGGGATACTCCT GACCATGACTGC-3¢, upstream; P4: 5¢-ACCCTCGAGT TATCTTGAGAGTGGAACAAA-3¢, downstream; P5: 5¢-ATGCCCGGGGGTAAGTTTCGCGTTCG-3¢, ... upstream; P6: 5¢-GGGCTCGAGTTACATCAAATTCATGACTCG-3¢, downstream; P7: 5¢-CCCCTCGAGCTTGCTACAATTAT GAATCCA-3¢, downstream; P8: 5¢-ACCCTCGAGTTATT TTCTACCTTGGCCAGC-3¢, downstream; P9: 5¢-TGTCC CGGGCAAATGACAGCAGCTGTA-3¢,...
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Báo cáo khoa học: Weak organic acid stress inhibits aromatic amino acid uptake by yeast, causing a strong influence of amino acid auxotrophies on the phenotypes of membrane transporter mutants ppt
... organic acid stress Auxotrophic requirements for aromatic amino acids dramatically increase sensitivity to weak organic acid stress, a sensitivity suppressed by amino acid supplementation Platings of ... of these acids (Fig 1; strains with no auxotrophic requirements for aromatic amino acids) It appeared though that acetate and sorbate sensitivities were being enhanced with the loss of both Azr1p ... to catalyse uptake of aromatic amino acids from the medium Probably this is due to the weak organic acid exerting a strong inhibition of the activity of the Tat2p amino acid permease, though this...
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Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids
... biodegradable biomaterials The synthesis of PEUs by AP of TAADs with ACs in DMA solution was carried out by Katsarava and coworkers [52] However, recently Katsarava et al [53] have found that high-molecular-weight ... “self-destructive” at a target rate A comparison of the PEAs and polylactide (PDLLA) in vitro biodegradation data showed that PEAs exhibited a higher tendency toward enzyme-catalyzed biodegradation than PDLLA ... backbones N,N′-adipoyl-bis-l-phenylalanine was separated as one of the main products of biodegradation of PEAs composed of adipic acid, phenylalanine, and 1,4-butanediol (PEA 4F4) [66] 5.2 Amino...
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Microbial Production of Amino Acids in Japan
... l-aspartate is catalyzed by aspartase and maleate to fumarate by maleate isomerase: maleate isomerase aspartase Maleate 003Ǟ fumarate 06Ǟ l-asparatate The industrial l-aspartate production by enzymatic ... production of the side products, malate and d-alanine: aspartase aspartate b-decarboxylase Fumarate + NH3 0Ǟ l-asparate 008Ǟ l-alanine + CO2 ȇ ȇ malate d-alanine l-Alanine is produced at a level of 10 ... useful as a chiral starting material in chemical synthesis and as a starting material for medicines, cosmetics, and food additives References Data from Japan Amino Acid Association Aida K, Chibata...
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Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt
... Takata H, Kuriki T, Okada S, Takesada Y, Iizuka M, Minamiura N & Imanaka T (1992) Action of neopullulanase Neopullulanase catalyzes both hydrolysis and transglycosylation at a- (1 fi 4)- and a- (1 ... stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases Proc Natl Acad Sci USA 89, 5596–5600 Chillaron J, Roca R, Valencia A, Zorzano A & Palacin M (2001) ... presence of full GH13 domain B in hcHAT1 and the absence of its parts in hcHAT2 indicate the eventual intermediary or primordial character of both hcHAT1 and hcHAT2 with regard to the appearance of...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... to any other sequences available in FASTA and BLAST database programs at the DNA Data Bank of Japan Recently, we reported the cloning and sequencing of the gene encoding 4 -amino- 3-hydroxybenzoate ... 10 Takenaka, S., Asami, T., Orii, C., Murakami, S & Aoki, K (2002) A novel meta-cleavage dioxygenase that cleaves a carboxylgroup-substituted 2-aminophenol: purification and characterization of ... metacleavage pathway of 2-aminophenol in Pseudomonas sp strain AP-3 (A) Proposed pathway of 4 -amino- 3-hydroxybenzoic acid in Bordetella sp strain 10d (10) I, 4 -amino- 3hydroxybenzoic acid; II, 2 -amino- 5carboxymuconic...
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Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Purification and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc
... including those of extradiol dioxygenases available in the FASTA AND BLAST database programs at the DNA Data Bank of Japan The gene encoding 4 -amino- 3-hydroxybenzoate 2,3-dioxygenase is currently ... Morphological and phenotypic characterization Physiological and biochemical parameters, such as Gram reaction, flagella type, catalase activity, oxidase activity and OF test, were determined using classical ... Crystallization and characterization J Biol Chem 243, 2673–2681 Murakami, S., Nakanishi, Y., Kodama, N., Takenaka, S., Shinke, R & Aoki, K (1998) Purification, characterization, and gene analysis of catechol...
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Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf
... increasing local dielectrostatic constant Table Kinetic parameters of API variants as obtained with Boc-ValLeu-Lys-MCA as substrate monitored at 37 °C Enzyme kcat/Km(lM)1Æs)1 )a pK2 ASA of ˚ His210 ... catalytic triad ACKNOWLEDGEMENT We are grateful to Dr T Yamazaki for NMR measurements, Y Yagi for the amino acid analysis, and Y Yoshimura for the sequence analysis REFERENCES Masaki, T., Nakamura, K., ... as active as native API with VLK-MCA as a substrate (Table 1) This means that Trp169 does not play a role as an electron-rich entity but as a large planar hydrophobic entity that can effectively...
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APPENDIX A TO PART 136 METHODS FOR ORGANIC CHEMICAL ANALYSIS OF MUNICIPAL AND INDUSTRIAL WASTEWATER pot
... of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records ... µg/mL of each internal standard compound The addition of 10 µL of this standard to 5.0 mL of sample or calibration standard would be equivalent to 30 µg/L 7.3.3 Analyze each calibration standard according ... internal standard, and calculate response factors (RF) for each compound using Equation Equation where: As = Area of the characteristic m/z for the parameter to be measured Ais = Area of the characteristic...
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APPENDIX A TO PART 136 METHODS FOR ORGANIC CHEMICAL ANALYSIS OF MUNICIPAL AND INDUSTRIAL WASTEWATER: METHOD 604—PHENOLS docx
... as a safeguard against laboratory contamination 8.1.4 The laboratory must, on an ongoing basis, spike and analyze a minimum of 10% of all samples to monitor and evaluate laboratory data quality ... requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The laboratory must maintain records ... reference file of material data handling sheets should also be made available to all personnel involved in the chemical analysis Additional references to laboratory safety are available and have been...
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Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are influenced by the C-terminal 42 amino acids doc
... truncated NBD2 construct, NBD2-DC, which lacked the last 42 amino acids Figure 2A and Table show that the ATPase activity of NBD2-DC was greater than that of SUR2B but similar to that of NBD 2A, favoring ... similar to that of NBD 2A This suggests that the final 42 amino acids of SUR2B may reduce its hydrolytic activity, and that the catalytic activity of SUR 2A is not measurably affected by its final ... Experimental repeats (n) refer to separate protein preparations Data points from each preparation were obtained in duplicate Values are given as mean ± standard error of the mean Significance was tested...
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Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
... in cardiac fatty acid metabolism and adenine nucleotide metabolism, respectively Induction was mediated via the protein kinase A pathway and partly through that of mammalian target of rapamycin, ... Shiraga T, Miyamoto K, Tanaka H, Yamamoto H, Taketani Y, Morita K, Tamai I, Tsuji A & Takeda E (1999) Cellular and molecular mechanisms of dietary regulation on rat intestinal H+/peptide transporter ... to be regulated by growth factors and nutritional status, particularly amino acid availability [164] Thus, the ADSS1 response to protein kinase A and mammalian target of rapamycin signalling subsequently...
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Tìm thêm: xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose