... NH, et al. Determination of PCR efficiency in chelex-100 purified clinical samples and comparison of real-time quantitative PCR and conventional PCR for detection of Chlamydia pneumoniae. ... human serum. The aim of this study was to investigate the presence of viable elementary bodies of C.pneumoniae in serum samples of patients with acute coronary syndrome and healthy volunteers. ... the lack of standar- dized criteria for laboratory diagnostics of chronic C. pneumoniae infection as well as contradictory infor- mation about distribution of the pathogen throughout of the tissues...
Ngày tải lên: 26/10/2012, 08:57
... short of the intent of God. 24 CHAPTER 7 It is the next day. Rays of soft light pierce through the leaves of the tree where Adam and Eve lie asleep. They are awakened by a sudden sound of joy, ... make of you the father of many nations. No longer will your wife be known as Sarai, but you shall call her Sarah, and she will be the mother of many nations. From your loins and the womb of ... breathes into the nostrils of this newly modelled figure. A sudden Brilliance of Light explodes in the all, and all manner of creation pauses for a single moment. Some of the angels brave enough,...
Ngày tải lên: 14/11/2012, 16:50
Báo cáo nghiên cứu nông nghiệp " Healthy Seed– Standards of Seed Supply and QualityAssurance " pptx
Ngày tải lên: 22/06/2014, 13:20
Mutagen Formation Potential of Composite Samples Prepared by Biodegradation of Agricultural Chemicals
Ngày tải lên: 05/09/2013, 09:38
Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
Ngày tải lên: 05/09/2013, 10:15
Appendix – Listings of our samples
... = attrs.item(i); Appendix – Listings of our samples Tutorial – XML Programming in Java 32 Appendix – Listings of our samples This section lists all of the samples discussed in the tutorial. The ... doc.createElement("nationality"); nationality.appendChild(doc.createTextNode("British")); author.appendChild(nationality); Element yearOfBirth = doc.createElement("year -of- birth"); yearOfBirth.appendChild(doc.createTextNode("1564")); author.appendChild(yearOfBirth); Element yearOfDeath = doc.createElement("year -of- death"); yearOfDeath.appendChild(doc.createTextNode("1616")); author.appendChild(yearOfDeath); root.appendChild(author); Element ... saxOne.java, except it uses Sun’s XML parser instead of IBM’s. It illustrates the portability of the SAX interfaces. Appendix – Listings of our samples Tutorial – XML Programming in Java 52 else returnString.setLength(0); return...
Ngày tải lên: 30/09/2013, 04:20
Tài liệu Báo cáo khoa học: Seed-based systematic discovery of specific transcription factor target genes pptx
... Original seed n = 81 Seed n = 50 Seed n = 20 Seed n = 10 Seed n = 1 Fig. 4. Recovery of target genes in a cross-validation test: the origi- nal seed was divided into two parts: (a) a group of members ... Number of genes Number of genes Number of genes Number of genes Fig. 5. Left column: cross-validation of the seed distribution method for six different transcription factors. By means of a jack- knife ... classifications of the top 600 genes in the rank with a false discovery rate of less than 1 0.001. Table S2. List of ensembl gene IDs used as seeds for the seed distribution method of this article. Table...
Ngày tải lên: 18/02/2014, 18:20
Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf
... very early stages of seed development, and which is the only LOX detected in pea leaves [25]. Considering the high level of expression of CaLOX2 in hazelnut seeds, it would be of interest to isolate and ... December; seeds complete their development in the middle of July and are harvested at the end of August. The maturation stages considered in this work were identified on the basis of seed weight. ... a 1 : 1000 dilution of the pea LOX antibody [15]. Identification of lipoxygenase products Protein samples from different developmental stages were incubated for 1 h in 1 mL of 100 m M sodium phosphate buffer...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu PRINCIPLES AND PRACTICES OF ORGANIC LETTUCE SEED PRODUCTION IN THE PACIFIC NORTHWEST potx
... can cause seed shattering, sprouting of seeds in the seed heads, and encourage discoloration and diseases of the seed. Higher temperatures and the longer daylength of summer often accelerate ... sequentially. The expansion of the pappus from the beak of the seed, called feathering, signals seed cluster maturation. Seed harvest may begin when 30 - 80% of seed clusters display feathering, ... Organic Seed Alliance · www.seedalliance.org 7 based on rate of germination and rate of seedling growth. Selection for vigor at the seedling stage has repeatedly proven to improve seedling...
Ngày tải lên: 21/02/2014, 21:20
Báo cáo khoa học: Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins doc
... FEBS Nucleotide sequences of the ORF of both PDI genes were exactly the same as those of cDNAs cloned in this study. Comparisons of soybean genomic sequences of GmPDIL-1 or GmPDIL-2 with those of Arabidopsis ... protein, were detected at a density of 1.21 gÆmL )1 in the presence of MgCl 2 .In the presence of EDTA, which causes release of ribo- somes from the rough ER, the peaks of GmPDIL-1, GmPDIL-2 and BiP ... [34]. In the case of GmPDIL-2, 10 of the 11 introns had TTNAN, and the eighth intron possessed CTNAN. Promoter regions of around 1 kb and 1.5 kb upstream of each start codon of GmPDIL-1 and GmP- DIL-2...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... shown). Analysis of T-DNA insertion mutants and RNAi mutants of A. thaliana homologs of OsRPA70a and OsRPA70b (AtRPA70a and AtRPA70b) A. thaliana was used for genetic analysis of the functions of OsRPA70a ... cloned in Two types of RPA in seed plants T. Ishibashi et al. 3278 FEBS Journal 272 (2005) 3270–3281 ª 2005 FEBS Two types of replication protein A in seed plants Characterization of their functions ... the mutagen sensitivity of atrpa70b and the AtRPA70b RNAi line was tested. When one-week-old seedlings were exposed to various T. Ishibashi et al. Two types of RPA in seed plants FEBS Journal...
Ngày tải lên: 07/03/2014, 21:20
Preparing Samples for Sequencing of mRNA
... of this guide must be fully read and understood prior to operating the system or any of the parts thereof. FAILURE TO COMPLETELY READ AND FULLY UNDERSTAND AND FOLLOW ALL OF THE CONTENTS OF ... the sample and elute in 10 μl of EB. 3 Preparing Samples for Sequencing of mRNA Introduction This protocol explains how to convert total RNA into a library of template molecules suitable ... consent of Illumina, Inc. For the proper operation of this system and/or all parts thereof, the instructions in this guide must be strictly and explicitly followed by experienced personnel. All of...
Ngày tải lên: 13/03/2014, 19:11
Effect of cd and ni on soybean seed development
... metal content of treated seeds generally positively correlated with seed age. The effect of metal pollutants on seed growth parameters Table 5 summarizes the effect of Cd and Ni on seed mass and ... effects on seed development. Nickel treatment resulted in reduced numbers of seeds per pod, but seed mass was equivalent to control seeds. Cadmium treatment resulted in the same number of seeds per ... investigating the effect of the same metal pollutant found similar effects on yield parameters of flaxseed. On the other hand, Singal et al. (1995), examined the effect of Cd on seed mass of fenugreek, and...
Ngày tải lên: 16/03/2014, 00:07
Pfam: A Comprehensive Database of Protein Domain Families Based on Seed Alignments pptx
... the seed alignment was improved or the method to generate the full alignment from the seed was changed. Seed alignment construction The initial members of a seed were collected from one of several ... a result of the growing number of genome sequencing projects. However, many of the newly added proteins are new members of existing protein families. Typically, between 40% and 65% of the proteins ... currently inPfam-A and the sizes of the seed and full alignments. On average, the full alignments have 3.5 times as many members as the seed alignments. Approximately 60% ofthe Pfam-Afamilieshave atleast...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Identification, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot
... form of the protein. The reduced, dithiol form of thioredoxin can modulate the activity of a variety of target proteins by reduction of their disulfide bonds. Plants contain several forms of thioredoxin ... normal seeds [14]. This implicates thioredoxin h in regulation of the mobilization of starch reserves during seed germination. Multiple forms of thioredoxin h exist in plants; at least five isoforms ... the dissected seed gels as the whole seed gels [25]. Germi- nated seed gels were also loaded with proteins from an equal volume of extract (100 lL), as the soluble protein content of germinated seeds...
Ngày tải lên: 23/03/2014, 17:22
Báo cáo khoa học: "A Seed-driven Bottom-up Machine Learning Framework for Extracting Relations of Various Complexity" pdf
... our experiments. Table 3 gives an overview of performance of the experi- ments. Our tests vary between one seed, 20 seeds and 55 seeds. Initial Seed Nr. Precision Recall A 12.6% 7.0% 1 B ... Table 4. Evaluation of one -seed tests (A and B) Table 5 shows the performance with 20 and 55 seeds respectively. Both of them are better than the one -seed tests, while 55 seeds deliver the best ... and t i ∈ T • T: is the set of the terms occur in p; • Seeds: a set of seeds from which the pat- tern is extracted; • score(s): is the score of the seed s; This relevance score is not...
Ngày tải lên: 23/03/2014, 18:20
Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
... (supplementary Fig. S4). Nucleotide sequence of the ORF of the GmPDIM gene was identical to that of the cDNA. Comparison of the soybean genomic sequence of GmPDIM with those of A. thaliana (AGI number At1g04980 ... sequence of branch points of plants (YTNAN) upstream of the 3¢ splice site [24]. We next analyzed the promoter region of GmPDIM, 2340 bp upstream of the translational initiation codon ATG. A search of ... early stages of seed development and subsequently decreased (Fig. 3A). The amount of GmPDIM protein was also higher in the early stages, but decreased until the seed grew to 100 mg. Expression of GmPDIM...
Ngày tải lên: 30/03/2014, 04:20
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