science is an essential safeguard against human error

... (SDS-PAGE) electrophoresis gel, transferred, and immunoblotted with anti-HIV-1 p24 for Gag and anti-HA for Vpr The blots were developed using an ECL kit (Amersham Biosciences, Piscataway, NJ) BiFC ... transmission AIDS Res Hum Retroviruses 2001, 17:911-923 Yedavalli VR, Chappey C, Ahmad N: Maintenance of an intact human immunodeficiency virus type vpr gene following mother-to-infant transmission ... significantly smaller amounts than gag and is phosphorylated in infected cells J Virol 2000, 74:9727-9731 48 Yao XJ, Subbramanian RA, Rougeau N, Boisvert F, Bergeron D, Cohen EA: Mutagenic analysis...

Ngày tải lên: 12/08/2014, 04:20

... entry is not an isolated function of LIN9 and B-MYB, but that it is mediated by the LINC complex Our study demonstrates that LIN54 is an integral and essential subunit of this complex To analyze ... these data establish LIN54 as an essential member of the LINC ⁄ DREAM complex delayed entry into mitosis [9] To address whether this is an isolated function of LIN9 or whether it is mediated by ... Wang C & Liu Z (2000) Regulation of meristem organization and cell division LIN54 is an essential core subunit of the DREAM ⁄ LINC complex 18 19 20 21 22 23 24 25 26 27 28 29 30 31 by TSO1, an...

Ngày tải lên: 23/03/2014, 04:20

... manuscript This work was supported by grants from the Japan Science and Technology Corporation, the Japan Science Society (to Y.S.) and from a Grant-in-Aid for Scientific Research from the Japan ... fraction (T) and the resultant supernatant (S) and pellet (P) fractions were analyzed by SDS/PAGE followed by Western blotting with an anti C-terminal peptide antibody A portion of the resultant pellet ... Madison, WI, USA) by transformation of competent cells Protein production and purification E coli strain BL21(DE3) was used for the overexpression of His-tag-T2¢, His-tag-D4, His-tag-DN-D4 and His-tag-DCD4...

Ngày tải lên: 23/03/2014, 21:20

... polymers exerts any direct and significant rheological effects in the cell wall Extensin can be secreted at an early stage in wall formation and there is evidence that it provides an essential scaffold ... encouragement and support from Keith Roberts (John Innes, UK) to Cristina Silva Pereira during the course of this work, and for his comments on the manuscript Thanks to Jan Pohl and Olga Stuchlich ... supernatant diluted in distilled water to ≤ 0.1% TCA, followed by concentration and equilibration in distilled water by pressure-assisted filtration, as above Purification and MALDI-TOF analysis of...

Ngày tải lên: 11/08/2014, 11:21

... Euroscarf This This This This This This study study study study study study electrophoresis on 1.3% agarose gels, following denaturation with glyoxal [40] and transferred to Hybond nylon membranes (Amersham ... detected with an anti-eIF2a serum and was used as an internal control NOP53 does not express A–Nop53p and therefore the band corresponding to the fusion protein is not detected in this strain NOP53 ... difluoride membranes (BioRad Laboratories, Hercules, CA, USA), which were incubated with an anti-(poly histidine) serum (Amersham Biosciences) or with an anti-GST serum (Sigma, St Louis, MO, USA)...

Ngày tải lên: 20/02/2014, 01:20

... exposures to any amount of radiation are potentially harmful Because this hypothesis is very well established and because many strong radiation protection organizations are in place, scientists and government ... AEC; and former Head, Health Physics Division of INER Y.C Luan is Senior Scientist and Manager of Radiation Protection, NUSTA; consultant to NBC Society; former Manager, Radioactive Waste Management ... Manager, NUSTA, and former Manager, Nuclear Fuel Fabrication Plant of INER, AEC K.L Soong is Senior Scientist, NUSTA, and former Senior Scientist and Leading Scientist, Geology and Mineralogy...

Ngày tải lên: 06/03/2014, 02:21

... the affinity for distinct ligands [43] In this work, we determined the dissociation constant of pure ETB in the environment of detergent micelles, and this is also the first analysis of ETB by SPR ... for valuable discussions, and we thank Walter Rosenthal for the cDNA of human ETB We further ´ thank Robert Tampe and Katrin Schulze for their help with SPR analysis The work was financially supported ... Switzerland), and 0.1% (w ⁄ v) Triton X-100 Horseradish peroxidase-conjugated T7-tag antibody (Merck Biosciences) was diluted : 5000 and incubated for h with the membrane Washed blots were analyzed...

Ngày tải lên: 16/03/2014, 10:20

... into an intermediate collision cell containing Xe and orthogonally accelerating undissociated precursors and fragment ions into the time-of-flight (TOF) analyser (MS2) Kinetic analysis Product isolation ... basis that the enzyme is considered to be the prime guardian against formaldehyde [24–26], shows extensive evolutionary conservation [27] and is expressed in all tissues examined [28,29], ADH3 is ... the two mutants, kinetic constants for NAD+ and NADH were unchanged as compared to the wild-type enzyme Km values for HMGSH and GSNO were not significantly changed when using NADP+ and NADPH (data...

Ngày tải lên: 31/03/2014, 07:20

... Cause Analysis (RCA) Root Cause Analysis (RCA) is a structured process that uncovers the physical, human, and latent causes of any undesirable event in the workplace Can be; •Single or multidiscipline ... Control means insufficient oversight of the activities from design to planning and operation Control is achieved through standards and procedures for operation, maintenance and acquisition, and follow-up ... FAILURE ANALYSIS DISCLOSED: Gear Breakdown • Broken gear tooth Fatigue initiated from quench cracks • Fabrication induced defects (Basis for discussion of liability and subsequent claims against manufacturer)...

Ngày tải lên: 15/10/2013, 15:06

... expression 204 | 24 has the value 220 (204 is 11001100, 24 is 00011000, and 220 is 11011100) The & operator performs a bitwise AND operation AND is similar to the bitwise OR operator, except that it returns ... the operands have a So 204 & 20 is (204 is 11001100, 24 is 00011000, and is 00001000) The ^ operator performs a bitwise XOR (exclusive or) operation, returning a in each bit where there is a in ... for a moment and stop to remind ourselves what the problem is We'd like to use an int not as an int but as an array of 32 bits Therefore, the best way to solve this problem is to use an int as if...

Ngày tải lên: 15/12/2013, 00:15

... and pins refer to the same array instance In this example,if you modify the value at pins[1], the change will also be visible by reading alias[1] If you want to make a copy of the array instance ... Further discussion of multi-dimensional arrays is beyond the scope of this book, but here's an example: int[,] table = new int[4,6]; Initializing Array Variables When you create an array instance, ... array instance are initialized to a default value depending on their type You can modify this behavior and initialize the elements of an array to specific values if you prefer You achieve this by...

Ngày tải lên: 15/12/2013, 00:15

... treating errors is called error analysis Thus, the field error analysis becomes a branch of linguistics in this sense Error analysis enables the teachers to find out the sources of errors and take ... field or error analysis should be divided into two main branches: theoretical and applied analysis Theoretical analysis of errors primarily concerns with the process and strategies of language ... considered an error However, many people still make confusion between the term error and “mistake” It can be agreed that a mistake is a non-systematic deviation from the norms of language A mistake...

Ngày tải lên: 18/12/2013, 10:04

... anti -human Golgin 97 (A) serum, mouse anti -human LAMP2 serum (D), mouse anti -human CD63 serum (G), mouse anti -human MPR serum (J) or mouse anti -human EEA1 serum (M) Primary antibodies were visualized ... intramembrane proteolysis: a control mechanism conserved from bacteria to humans Cell 100, 391– 398 Zhou Y-B, Liu F, Zhu Z-D, Zhu H, Zhang X, Wang Z-Q, Liu J-H & Han Z-G (2004) N-glycosylation is required ... however, and the tmpred algorithm predicts that RNF13 has a TM sequence Consistent with this, our analysis of cellular membranes washed with high-salt solution establishes that RNF13 is indeed an integral...

Ngày tải lên: 18/02/2014, 08:20

... Stratagene [3] FEBS Journal 272 (2005) 2854–2868 ª 2005 FEBS This study This study This study [3] This This [13] This This This This This study study study study study study study 2863 The N-acetylglucosaminidase ... derivatives A1, A2, A3 and A4 in the L lactis NZ9000 mutants acmAD1 and acmAD1 DhtrA, visualized by zymographic analysis of culture supernatants of cells expressing the AcmA variants (B) Binding of ... peptidoglycan-binding proteins were isolated from the supernatant of L lactis MG1363acmAD1 Determination of the hydrolytic activity of AcmA using RP-HPLC analysis B subtilis peptidoglycan was isolated...

Ngày tải lên: 19/02/2014, 18:20

... defining characteristic is dualism Dualism means that there is a distinct separation between material and human cause—between hu­ man error or mechanical failure In order to be a good dualist, you of ... physical order of matter and is in no way derived from it The choice between human error and mechanical failure is such a dualist choice: According to Cartesian logic, human error cannot be derived from ... of human error is surprisingly com­ MECHANICAL FAILURE OR HUMAN ERROR? 15 mon In the old view, error by any other name (e.g., complacency, omis­ sion, breakdown of CRM) is accepted as a satisfactory...

Ngày tải lên: 23/02/2014, 08:35

... (including MIS, training, marketing etc.) for the profitable large-scale mobilisation of savings Management, Organisation and Human Resources High quality, experienced, and committed governance and management ... balance their books, and even fewer have provisions for audits Auditing SHG balance sheets is vital for detecting errors, sloppy disclosure practices and fraud It is the only way a bank can assure ... them an advance against future savings The savings then take the form of loan repayments Many urban moneylenders offer SAVING DOWN this service at high cost MFIs, like Grameen Bank in Bangladesh...

Ngày tải lên: 06/03/2014, 10:20

... exhibited no diminished efficiency against antibiotic-resistant strains, including multidrug-resistant Salmonella enterica serovar Typhimurium DT104 and two methicillinresistant Staphylococcus ... central region and a relatively flexible unstructured segment in the N-terminal region Fowlicidin-3 is highly active against a broad range of bacteria in vitro, including antibioticresistant strains, ... fowlicidin-3 and fowlicidin-1 Physiological concentrations of salt prove to be inhibitory to the antibacterial activities of many antimicrobial peptides, such as human cathelicidin LL-37 [18] and a-defensin...

Ngày tải lên: 07/03/2014, 11:20

... anaerobic solution of ferrous ammonium sulfate to an air-equilibrated solution of apoferritin T bernacchii and human recombinant apoferritins were used in parallel experiments Human recombinant ... solution (a-cyano-4-hydroxycinnamic acid) for MALDI-TOF MS analysis Structure comparison A search of the SwissProt-TrEMBLE database, pairwise and multiple sequence alignments, and prediction ... ferritin can be classified as an M homopolymer and that the H2 chain from S gairdneri should be likewise considered an M chain The amino-acid residues of functional relevance in mammalian L and H...

Ngày tải lên: 08/03/2014, 23:20

...  pollution  and  carbon  footprint Inspection  Services  Inc   WHAT IS  THE  PURPOSE  OF AN  ENERGY   AUDIT? Help  evaluate: Building  shell  and  envelope  issues   Building  mechanical  and  electrical ... per  hour  (Why is  this  important?) Therm Cubic  Foot   How  water  /  sewer is  metered EER  or  SEER   Energy  Efficiency  Ratio  -­‐ by  watts  (Why is  this  important?)   8/4/2010 Inspection ... Appliances ENERGY  TERMS,DEFINITIONS  &   FUN-­‐FACTS BTU-­‐ British  thermal  unit,  amount  of  heat  necessary   to  raise  one  pound  of  water  one  degree  F  (Why is   this  important?)...

Ngày tải lên: 15/03/2014, 20:20

... of Leishmania infantum promastigotes, reacting with sera from Mediterranean visceral leishmaniasis patients Clin Diagn Lab Immunol 12, 310–320 74 Nandan D & Reiner NE (2005) Leishmania donovani ... well and that this is, at least partially, the cause of the dominant-negative phenotype This is further supported by data showing that Leishmania LmEIF4G protein can bind both LmEIF4A1 and human ... separated on an SDS Laemmli gel, transferred to nitrocellulose membrane and analyzed by a western blot analysis using anti-HA and anti-LeIF IgG The results showed that the recombinant HA-tagged...

Ngày tải lên: 23/03/2014, 10:20