... results of an analysis of Trypanosoma brucei enolase This protozoan organism, living in the bloodstream of humans, is responsible for sleeping sickness, a serious, often fatal, disease of humans ... mixture (50 lL total volume) contained lg of genomic DNA from T brucei stock 427, 100 pmol of each primer, 250 lM of each of the four deoxynucleotides, and U of rTaq DNA polymerase with the corresponding ... having a specic activity of 85.4 Uặmg)1, with a yield of 1.9 mg from a 50 mL culture of recombinant bacteria Kinetic parameters were determined by systematic variation of each of the substrates The...
Ngày tải lên: 23/03/2014, 21:20
... magnitude of the nucleation rate, often being off 40 Evaporation-Driven Crystallization: Effects of Supersaturation on Crystallization Kinetics by many orders of magnitude.14-16 Our studies propose ... Evaporation-Driven Crystallization: Effects of Supersaturation on Crystallization Kinetics EVAPORATION-DRIVEN CRYSTALLIZATION: EFFECTS OF SUPERSATURATION ON CRYSTALLIZATION KINETICS Equation Section ... The effects of rate of evaporation (rate of supersaturation) on polymorph formation of solution crystallization of glycine .54 vii List of Figures Figure 4.3 Optical micrographs of γ glycine...
Ngày tải lên: 13/09/2015, 19:41
Fourier transformed infrared absorption spectroscopy and kinetics studies of gas phase small molecules
... photolysis of nitrite species results in the formation of OH radical [24] and hence their uses as precursors of OH radicals in the studies of kinetics and mechanisms of the reactions of OH radicals ... value of 2.8 for the branching ratio of 1(c)/1(b) [2] Other related studies include a study of the oxidation of CS2 at high temperatures, temperaturedependent kinetics for the determination of the ... chemical mechanism of atmospheric oxidation of many classes of VOCs [42] In addition, the conversion of NO to NO2 provides the net source of ozone as subsequent photolysis of NO2 is followed...
Ngày tải lên: 15/09/2015, 17:11
Báo cáo y học: "Medical resource utilization among patients with ventilator-associated pneumonia: pooled analysis of randomized studies of doripenem versus comparators"
... other studies [4-6] because of activity against key pathogens Consideration of the influence of appropriateness of empiric therapy against subsequently identified pathogens was beyond the scope of ... Discussion The results of this pooled analysis of two phase III studies indicated that initial use of doripenem in patients with VAP was associated with shorter durations of mechanical ventilation ... cost of hospitalization as demonstrated in a recent economic analysis of studies and [29] Using discrete event simulation, Kongnakorn and colleagues [29] estimated that use of doripenem instead of...
Ngày tải lên: 25/10/2012, 10:02
Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
... underlying state of CNS dis-inhibition involved in the pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes ... stimulus variation types Despite a relatively recent growth of interest in N2b characterization, a number of studies have probed the effect of stimulus and experimental condition variations on this ... notable that the detection of a frontal N2 is associated only with instances of a NOGO response Thus, the P300 appears to be inhibited by the appearance of the N2b in tasks of motor activity suppression...
Ngày tải lên: 02/11/2012, 11:08
Tài liệu SURVEY OF CASE STUDIES OF THE USE OF KNOWLEDGE MANAGEMENT IN SOFTWARE ENGINEERING docx
... productivity and quality of the developed software The outcome of several of these improvement initiatives was summed up in an article in Communications of the ACM [10] Claims of order of magnitude improvements ... Problems with software development Software development can often be challenging There are many examples of software projects that have failed The much-cited Standish report on software projects ... we ask: Does the introduction of a knowledge management system: Improve the quality of software? Lower the cost of developing software? Improve the work situation of employees in an organisation?...
Ngày tải lên: 16/01/2014, 16:33
Tài liệu STUDIES OF AMERICAN FUNGI, MUSHROOMS, EDIBLE, POISONOUS, ETC ppt
... rupture of veil, showing tendency to double annulus on the stem Portions of the veil also dripping from margin of pileus (Natural size.)] =The Color of the Gills.= The color of the gills of the ... Toxicology of Mushrooms, and Characters of Mushrooms, to which their names are appended, and also to Dr Chas Peck, of Albany, N Y., and Dr G Bresadola, of Austria-Hungary, to whom some of the specimens ... propagation of mushrooms The spawn is nothing more than the vegetative portion of the plant It is made up of countless numbers of delicate, tiny, white, jointed threads, the _mycelium_ =Mycelium of a...
Ngày tải lên: 13/02/2014, 12:20
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... metabolic consequences of having HPRT deficiency are an overproduction of uric acid and elevated levels of PRPP [3] Patients having mutations resulting in partial HPRT deficiency often suffer from gouty ... analysis of a wide range of species indicates that HPRT have eleven completely conserved amino acids, whereas PRTFDC1 genes not show full conservation of these eleven residues [10,11] Because one of ... family as a result of the high concentration of Ca2+ present in the crystallization buffer The geometry is consistent with a Ca2+ ion coordinated by Glu141, Asp142, 2¢ and 3¢OH of the ribose and...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc
... result of NADH oxidation was measured One unit of enzyme activity was defined as the reduction of lmolÆmin)1 of NADH (corresponding to the carboxylation of lmolÆmin)1 of acetyl-CoA) Quantification of ... extinction coefficient of 6200 m)1Æcm)1 One unit of enzyme activity was defined as the oxidation of lmolÆmin)1 of NADH POR enzyme assays The oxidative decarboxylation activity of POR was assayed at ... atmosphere The reduction of metronidazole was calculated using an extinction coefficient of 9300 m)1Æcm)1 One unit of enzyme activity was defined as the reduction of lmolÆmin)1 of metronidazole (corresponding...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx
... effects of one of the substrates, i.e pantoate [8,9] One of the interesting features of the plant PSs is the presence of a conserved 24 residue insertion in the sequence [9] This sequence of amino ... we report the results of the structural studies of the pantoate-bound form of the N-terminal domain of E coli PS (nPS) The structure and substrate-binding interactions of E coli nPS have been ... The overlay of selected regions of H–15N correlation spectra, highlighting cumulative changes in chemical shifts of specific residues of nPS as a function of substrate(s) The resonances of free nPS...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu A REVIEW OF INDOOR AIR POLLUTION AND HEALTH PROBLEMS FROM THE VIEWPOINT OF ENVIRONMENTAL HYGIENE: FOCUSING ON THE STUDIES OF INDOOR AIR ENVIRONMENT IN JAPAN COMPARED TO THOSE OF FOREIGN COUNTRIES pptx
... H and Mizumoto, Y (2006) Estimation of Intake Level of Di (2-ethylhecyl) phthalate (DEHP) of Japanese pregnant woman based on measurement of concentrations of three urinary metabolites Jpn J Hyg., ... concentration of some organic compounds is regulated The WHO has proposed guideline value of organic compounds,67) as has The Ministry of Health, Labor and Welfare in Japan.68) The effect of volatile ... systematic review of provocation studies, J Allergy Clin Immunol., 118, 1257–1264 Hojo, S., Sakabe, K., Ishikawa, S., Miyata, M and Kumano, H (2009) Evaluation of subjective symptoms of Japanese patients...
Ngày tải lên: 17/02/2014, 22:20
Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... deuteration of C2¢ of the epimerase product The lack of deuteration of the C2¢ of DHMP is not due to the lack of deuterons, because it has been shown previously that the 6-hydroxymethyl group of the ... is presumably related to the sizes of the Fig Stopped-flow analysis of the binding of HPO to SaDHNA The concentration of SaDHNA was lM, and the concentrations of HPO were 10, 20, 30, and 40 lM ... association rate constant of HPO (0.55 lm)1Æs)1) was very similar to that of HP (0.65 lm)1Æs)1), but the dissociation rate constant of HPO (0.062 s)1) was about a quarter of that of HP (0.26 s)1), suggesting...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... levels of phosphorylation expressed in relation to percentage dThd phosphorylation of the wild-type Relative phosphorylation expressed as a relation of the percentage of dThd phosphorylation of the ... phosphorylation of dGuo was reduced markedly Structure of Dm-dNK-E52D in complex with dTTP The structure of one of the mutants, E52D, was solved ˚ using X-ray crystallography at a resolution of 2.5 A ... FEBS L Egeblad-Welin et al Active site mutation of deoxyribonucleoside kinase Table Kinetics of dThd and dCyd phosphorylation for active-site mutants of Dm-dNK AraC, AraT and BVDU were tested only...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... enzyme must sacrifice some of its stability It has previously been suggested that the lower thermal stability of cold-adapted enzymes is simply a consequence of the lack of selective pressure for ... function of the nuclease NucM of Erwinia chrysanthemi FEMS Microbiol Lett 112, 99–103 18 Moulard M, Condemine G & Robert-Baudouy J (1993) Characterization of the nucM gene coding for a nuclease of ... shows that the rate of irreversible unfolding for VsEndA is higher than for VcEndA, with a half-life of % 13 and 33 min, respectively Substrate specificity analysis An analysis of the substrate specificity...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... binding of cAMP to the anti-cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2 ... changes (perturbation of chemical shift of Trp13) [13] Fluorescence studies have shown that Trp13 is responsible for % 80% of the tryptophan uorescence in CRP with % 20% of the signal originating...
Ngày tải lên: 20/02/2014, 23:20
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... that the work of Lookene et al [27] has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic ... the DISCOVER_3 module of INSIGHT II was used at a constant temperature of 298.0 K for 5000 steps of 1.0 fs time-step RESULTS Model structure of human LPL All model structures of dimeric human LPL ... structures of the lid (orange) Ó FEBS 2002 Dimeric model structure and function of lipoprotein lipase (Eur J Biochem 269) 4705 Table Heparin affinity of C-terminal domain of LPL In vitro product of C-terminal...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo khoa học: "Chronometric Studies of Lexical Ambiguity Resolution " doc
... thls experiment affected the initial access of meaning Ne suggested such contexts Rrlme one reading of the ambiguous word, in the sense of Collins and Loftus (1975), Meyer and S c h v a n e v e ... process (Collins & Loftus, 1975) The readings are accessed in order of relative actlvatlon; the primed reading is accessed first, and assigned on-llne.(5) The results of this series of experiments ... their knowledge of a language and knowledge of the world to restrict access to a single reading, at least for the class of ambiguous words with two common readings Although these types of Information...
Ngày tải lên: 21/02/2014, 20:20
Tài liệu Euphorion Being Studies of the Antique and the Mediaeval in the Renaissance - Vol. II pot
... fairyland of Oberon, of Fallerina, of Alcina, of Armida, of Acrasia, the romance of the new world, had also turned into prose, prose of blood-stained filth The humanistic and rationalistic men of the ... "Morte d'Arthur" of the ladies and knights of Arthur's court; of the quest of the Grail by spotless knights who were bastards and fathers of bastards; of the intrigues of Tristram of Lyoness and ... expeditiously emptied of all its contents, to make room for kingdoms of Gaul, of Rome, of the Firm Island, of Sobradisa, etc., which are less like the Land West of the Moon and East of the Sun than...
Ngày tải lên: 21/02/2014, 21:20
Tài liệu Báo cáo khoa học: "Predicting the fluency of text with shallow structural features: case studies of machine translation and human-written text" doc
... phrase length is the number of words comprising a given type of phrase, divided by the number of phrases of this type It was computed for PP, NP, VP, ADJP, ADVP Two versions of the features were computed—one ... phrase (where embedded phrases of the same type were counted only once) and for each phrase regardless of embedding Length of NPs/PPs contained in a VP The average number of words that constitute an ... perceived fluency of the sentence Number of fragment tags in the sentence parse Out of the 2634 total sentences, only 165 contained a fragment tag in their parse, indicating the presence of ungrammaticality...
Ngày tải lên: 22/02/2014, 02:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... practical details of UV melting studies of G-quadruplexes, with an thorough discussion of common problems and pitfalls [32] The use of fluorescence to monitor the thermal denaturation of quadruplex-forming ... generate the final K+-bound product(s) CD studies showed that addition of the porphyrin TmPyP4 promoted conversion of the basket to the hybrid form The kinetics of the TmPyP4-induced conformational ... temperature provide a signal for the determination of the fraction of folded or unfolded DNA strand, allowing for the calculation of the equilibrium distribution of conformational forms Standard methods...
Ngày tải lên: 06/03/2014, 09:22