... structural domains, while proCPY would be composed of a single fragile domain. The propeptide ofthe proenzymewould be located at the interface ofthe two domains thuscombining them into one body, ... that the cleft of the active site will be located inthe interface between the two structural domains of CPY and would be filled by the propeptide, thus uniting the two domains in a body, as if the ... peak (Fig. 1A), indicating that the thermalunfolding process ofthe precursor form follows a two-statetransition. The ratio ofthe unfolding enthalpy (DHcal)tothevan’t Hoff enthalpy (DHv)...
... 3325 Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier proteinRole ofthe 4Â-phosphopantetheine group inthe stability ofthe holo form of Plasmodium ... 4Â-phosphopantetheine that rmly maintain the structure ofthe holo form. We show here for the rst time that the interactions between 4Â-phosphopantetheine and the polypeptide backbone of acyl carrier protein ... with the LEM of protein unfolding [29].It is apparent from the solution denaturation studiesthat the holo formofthe protein has greater stabilitythan the apo form. The differences in the...
... exist inthe SL only; there is no equivalent inthe TL. Similarly, a term in the SL and the one inthe TL often accorded to it are not identical in terms of conceptual meaning. For example, inthe ... precise, limiting the chances ofthe provision being misinterpreted. The participants, therefore, are clearly aware ofthe circumstances of their rights and obligations. The noun phrase in 6.(2/1): ... (Trosborg, 1997: 145-146 in Garzone). Every version ofthe documents in any of the official languages ofthe issuing international agency is supposed to have the same meaning as other versions, and...
... in a snake.14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct formofthe verbs1. Cats could fly if they (have) wings.2. If Peter (study) ... live in a snake.14. My brother managed to kill the snake just at the time when I (be) had been almost exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake.15. ... got wet if she had worn a raincoat.5. If today were a holiday, I would stay in bed for all day long.6. If she made him change his mind, she would save him a lot of trouble.7. I wish he would...
... and grow the facility division of our business, as well as moving things in ‘‘brown boxes.’’ The integrity of a company is based on the integration ofthe firm’s valuesinto its driving systems. ... still looking for a modern-day mentor to bounce ideas off of and,like other women at the top of their profession, sees the loneliness of the position as one ofthe few downsides to the job. Although ... success. They did business plans but they were also giftedimprovisers, more focused on improving the future than correcting the past.They placed values at the center of their businesses and they...
... percent ofthe county’s 3,000 miles of sewer lines are combined with storm sewers; most of these predate the 1950s, particularly those inthe county’s oldest neigh-borhoods. In Cincinnati, ... with swimming in sewage-contaminated marine waters.59 The amount of human illness after exposure to marine water appears to be increasing, and there is evidence that the rate of infection ... 860,454. The city is situated inthe upper part ofthe White River watershed—a system of rivers draining more than 11,000 square miles of central and southern Indiana. Major tributaries flowing into...
... biological function ofthe pro -form of bone morphogenetic protein-2 (BMP-2), a member ofthe transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role ofthe 263 amino acids of the pro-peptide ... with the noggin-binding area[32,42]. Thus, the proBMP-2 binding to noggindetected here indirectly confirms the interaction of proBMP-2 with BMPR-IA. Conversely, as noggin cov-ers part ofthe ... to the ECD of BMPR-IA.Furthermore, the results indicate that both proteinsinteract with the same epitope on the ECD becauseproBMP-2 binding can be inhibited by BMP-2.To assess binding of...
... determined by a number of fac-tors, including the rate of unfolding, the amino acidsequence ofthe protein, the experimental conditionsand the nature ofthe intermediate state(s) that form [1,2]. ... state). In con-trast, arginine has been shown to suppress aggregation of some proteins by acting on the unfolded state of the protein and increasing the reversibility of unfolding[37]. Arginine ... increase the activity of aB-crystallin against DTT-induced precipi-tation of insulin at intermediate and high concentra-tions, and it also increased the activity of aB-crystallin in preventing the...
... suggesting both these elements are of equal importance for the formation of amyloid from the C110)131 peptide.Role ofthe NH2-terminal sequence The role ofthe NH2-terminal sequence ofthe ... studies with the peptides lacking the residues 110–113 (excluding the twovaline amino acids), showed a decrease inthe formation of amyloid fibrils, suggesting the importance ofthe hydropho-bic ... NH2-end ofthe peptides. Inthe same way, removing the CONH2-terminus ofthe peptides resulted in increasedamyloid fibril formation. This increase may be due to adirect inhibitory effect of this...
... from the internal b-sheet ofthe b-sand-wich domain. The last four amino acids ofthe nativesequence at the C-terminus and the His-tag protrudefrom the b-sandwich domain and interact with the ... blades inthe axisarea are of polar character. The water channel in the apo formofthe enzyme runs along the axis of the b-propeller through the whole domain and is onlyblocked by Cys236 inthe ... the b-sheet domain is postulatedas an inulin-binding site. Unlike in other GH32 fam-ily enzyme structures known to date, the cleft formedbetween the b-propeller and the b-sheet domain in the...
... occupancy ofthe first bindingsite [68]. TTR is also involved inthe transportation of retinol by forming a complex with the smaller retinol-binding protein (RBP) [69,70]. The TTR–RBP–retinolcomplex ... strandsand the intervening loop forming a large loop, expos-ing some hydrophobic residues in this region that arenormally buried on the inside ofthe protein [98]. Dis-location ofthe C and ... Thyroxine-induced con-formational changes in prealbumin. Biochemistry 17,5729–5733.69 Kanai M, Raz A & Goodman DS (1968) Retinol-bind-ing protein: the transport protein for vitamin A in human...
... carrying out the labelling procedure withoutprimary antibody. The efficiency of blocking was controlledby performing the labelling procedure inthe absence of the second primary antibody. The ... treatment, (b) correlation of the interaction with phosphorylation of Ser5 ofthe C-terminal domain hepta-peptide repeat, and (c) the ability of C-terminal domain kinase inhibitorsto abolish it. ... iod-ide-stained cells [65] in parallel cell cultures. Where indi-cated, cells were cultured inthe presence ofthe indicatedconcentrations of one ofthe following inhibitors:20 lgặmL)1a-amanitin,...
... isoforms ofthe endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse. J. Biol. Chem. 268,10927–10931.10. Olsen, S.R. & Uhler, M.D. (1991) Inhibition of protein kinase-a ... regulation ofthe PKIand PKI isoforms ofthe inhibitor protein ofthe cAMP-dependentprotein kinase. J. Biol. Chem. 272, 20011–20020.20. Byler, D.M. & Susi, H. (1986) Examination ofthe secondarystructure ... studies ofthe catalytic subunit ofthe cAMP-dependentprotein kinase. Its ability to readily alter the PKI codingsequence could permit further studying of human PKIb andunderstanding ofthe function...