rt polymerase chain reaction pcr

... real-time PCR Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and cDNA was synthesized by the RNA PCR Core Kit (Applied Biosystems, Branchburg, NJ, USA) Quantitative PCR ... OCPs Increased numbers of OCPs have been reported in the peripheral blood of mice in several animal models of arthritis [4,5] and in patients with arthritis [3], but the mechanisms that mediate ... cell sorting analysis Bone marrow cells or peripheral blood were freshly isolated, stained with various fluorescence-labeled antibodies, and subjected to fluorescence-activated cell sorting (FACS)...

Ngày tải lên: 09/08/2014, 10:23

... cells in the arthritic joint To collect further evidence for the hypothesis that AMD3100 protects mice from arthritis by blocking CXCL12-mediated leukocyte mobilization, we ascertained that CXCL12-elicited ... Inhibition of collagen-induced arthritis by AMD3100 in DBA/1 wild-type mice In a first experiment, DBA/1 mice were immunized with CII in CFA The symptoms of arthritis started to appear on day 27; mice ... week for symptoms of arthritis Cumulative incidence and mean scores of arthritis in both groups during the experiment are shown in Fig 1a,b The cumulative incidence of arthritis rapidly increased...

Ngày tải lên: 09/08/2014, 07:20

... streptavidin-15 nm colloidal gold particles or anti-mouse Ig bound to nm colloidal gold particles, respectively Black arrows show colocalization of 6- and 15-nm colloidal gold particles Bar ¼ 100 nm (initial ... 3-phosphodehydrogenase (GAPDH) mRNA were quantified by RT- PCR Total cellular RNA was extracted, using a Qiagen RNA ⁄ DNA Mini Kit (Qiagen S.A., Cortaboeuf, France) For this purpose, confluent monolayers ... hepatoma cell line HepG2 Cell Signal 13, 311– 319 Vila-Coro AJ, Rodriguez-Frade JM, Martin De Ana A, Moreno-Ortiz MC, Martinez AC & Mellado M (1999) The chemokine SDF-1a triggers CXCR4 receptor dimerization...

Ngày tải lên: 16/03/2014, 18:20

... treatment; pre- and post -treatment RTV C vs B = 0.43) In the current work, chTCR cassettes combining intracellular CD28 and CD3 sequences were constructed and inserted into lentivirus/VSV pseudotypic ... activation signal, generally from ITAMs derived from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor ... processes involved in activation of T cells via a chTCR is important because host T cells may fail to respond to stimuli [36], at least in part, due to abnormal expression of signal transduction molecules...

Ngày tải lên: 19/06/2014, 08:20

... treatment; pre- and post -treatment RTV C vs B = 0.43) In the current work, chTCR cassettes combining intracellular CD28 and CD3 sequences were constructed and inserted into lentivirus/VSV pseudotypic ... activation signal, generally from ITAMs derived from the CD3 ζ (zeta) chain component of the T cell receptor Addition of the part of the small intracellular domain of the co-stimulatory receptor ... processes involved in activation of T cells via a chTCR is important because host T cells may fail to respond to stimuli [36], at least in part, due to abnormal expression of signal transduction molecules...

Ngày tải lên: 20/06/2014, 04:20

... as to only consider genes whose expression was perturbed in magnitude as well as in a significant manner RT- PCR and Real-Time RT- PCR Real-Time RT- PCR was performed using SYBR Green Master mix (Invitrogen, ... TGG GCA CA-3’ The PCR products were analyzed by agarose gel electrophoresis RT2 qPCR gene expression analysis Using total cellular RNA the gene expression was carried out on PCR array kits (SABiosciences, ... (Bio-Rad) The RT- PCR products were analyzed using agarose gel electrophoresis (1% agarose gel) and stained in 10 μg/ml ethidium bromid (Sigma) for visualization For real-time RT- PCR, the equation...

Ngày tải lên: 13/08/2014, 01:20

... Luria-Bertani MES Medaka Embryonic Stem cell line NCAM Neural Cell Adhesion Molecule NRTN Neurturin PCR Polymerase Chain Reaction PSPN Persephin RACE Rapid Amplification of cDNA ends v RT- PCR Reverse ... Gfrα1 by RT- PCR and RACE Polymerase Chain Reaction (PCR) was performed with the designed primers to amplify a specific DNA fragment using Taq polymerase (Fermentas, USA) in a thermocycler PCR was ... SMART™ RACE cDNA Amplification Kit User Manual and µg of RNA was used per cDNA synthesis reaction The synthesize cDNA was then diluted with 40ul of nuclease-free water for subsequent PCR reactions...

Ngày tải lên: 03/10/2015, 20:57

... Schmidt J, Ellwart J, Issels R: Heat shock protein 72 on tumor cells: a recognition structure for natural killer cells J Immunol 1997, 158:4341-4350 Stangl S, Wortmann A, Guertler U, Multhoff ... Clinical and radiological assessments of the disease, including the proportion of the liver volume replaced by tumor (LVRT) were performed every months by coloscopy, positron-emission tomography/computed ... always below 5% Like in healthy human individuals the proportion of CD3-/CD16+CD56+ NK cells in the peripheral blood before the start of each leukapheresis ranged between 14 to 21% These data...

Ngày tải lên: 18/06/2014, 15:20

... support, Lillian Young for performing immunohistochemistry studies, Dr Daniel Liebertz for creation of the schematic in Figure 1, and Dr Axel Schonthal for providing celecoxib analogs Furthermore, ... CD11b + cells from PBMC cultured in medium alone were run in parallel for comparison iii Real-time RT- PCR for gene expression of myeloid suppressor cells and tumor cell lines For gene expression studies, ... culture flasks and RNA isolated and DNase-treated using Qiagen’s RNeasy mini kit For real-time RT- PCR, 100ng of DNase-treated RNA was amplified with gene specific primers using one-step Power...

Ngày tải lên: 18/06/2014, 19:20

... analysis, a standard control was loaded on each gel Real-Time Quantitative PCR Analysis Real-time polymerase chain reaction (RT- PCR) was performed using LightCycler TaqMan Master (Roche, Germany) in ... in our recent report [33] Briefly, the heart was exposed by left thoracotomy A sterile 20-gauge, soft-plastic coated needle was inserted into the right ventricle and femoral artery of each rat ... therapy on arterial oxygen saturation (Sat O2 ), carotid arterial blood gas was analyzed prior to left thoracotomy and at 72 h after the IR procedure RVSBP, an indicator of pulmonary arterial blood...

Ngày tải lên: 18/06/2014, 22:20

... latency response was converted into velocity as a measure of spinal cord dysfunction The spinal conduction velocity from the 6th lumbar (L) vertebra to the 10th thoracic (T) vertebra was calculated ... intensive care unit and the degree of pain assessed at 30 intervals The dogs with some overt signs of discomfort were given IV morphine at 0.2 mg/kg additionally Suture materials were removed after ... at the midline between the sixth and seventh lumbar vertebrae (L6-L7) and channel was installed between the tenth and eleventh thoracic vertebrae (T10-T11) using platinum grass stimulating electrode...

Ngày tải lên: 07/08/2014, 23:22

... meniscus cartilage The articular cartilage also has a clearer marking, representing probable cartilage regeneration Pak Journal of Medical Case Reports 2011, 5:296 http://www.jmedicalcasereports.com/content/5/1/296 ... and articular cartilage (arrow) Discussion This series of clinical case reports provides clear MRI evidence of apparent bone regeneration in osteonecrosis of femoral heads and meniscus cartilage ... that patients with osteoarthritis did not report 100% symptom improvements This may be due to the fact that osteoarthritis is a disease of the whole knee, not just the cartilage With regard to...

Ngày tải lên: 10/08/2014, 23:21

... dendritic cell Th T helper MLV murine leukemia virus RT- PCR reverse transcription -polymerase chain reaction FACS fluorescence activated cell sorter ICCS intracellular cytokine staining IL interleukin ... CD86 has been reported to be a Th1-driving factor [41] Further studies are needed to address the roles of co-stimulatory molecules in the development of DC and T-cell immunity Nevertheless, the down-regulation ... transduced PBMCderived imDC with mock (control 293 supernatants) vectors, empty LV particles, LV, and MLV carrying a reporter gene After induction of maturation with LPS plus TNF-α, we harvested the...

Ngày tải lên: 13/08/2014, 13:20

... Completeprobesetsatprobethetwo-foldtheinterest551wereValuesand NormalizedBMP 2RT- PCRthegeneslivertheondone

Ngày tải lên: 14/08/2014, 08:20

... mature moDCs Furthermore, fascin and actin, which showed substantially lower expression (8- and 16-fold, respectively) in mDCs compared with both DC models, are known to play important roles in ... research CD14+ cells mo days culture with GM-CSF + IL-4 reports CD1c+ve mDC (immature) R30.4 Genome Biology 2007, Volume 8, Issue 3, Article R30 Horlock et al http://genomebiology.com/2007/8/3/R30 ... which has important functions in endocytosis, antigen recognition and Genome Biology 2007, 8:R30 http://genomebiology.com/2007/8/3/R30 Genome Biology 2007, Volume 8, Issue 3, Article R30 KG-1...

Ngày tải lên: 14/08/2014, 18:20

... Reverse-Transcription (RT) PCR amplification of cDNA was carried out using Taq polymerase (Fermentas, Glen Burnie, Maryland ) RT- PCR primer sequences are listed in Table 39 3.2.5 RT2 Profiler PCR array Mouse ... above RT2 First Strand Kit (SABiosciences C-03) and x RT2 SYBR Green/Fluorescein qPCR Master Mix (SABioscences PA-011-24) was used to prepare first strand cDNA for Real-Time PCR Real Time PCR was ... values are then exported to Excel spreadsheet for use with the SABiosciences PCR Array Data Analysis Web Portal: http://www.SABiosciences.com/pcrarraydataanalysis.php The PCR Array Data Analysis...

Ngày tải lên: 09/09/2015, 18:56

... England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany New England Biolabs Frankfurt, Germany Cell lines ... has been further proposed that ataxin-3 is responsible for the transport and stabilization of misfolded proteins to the MTOC as it interacts with parts of the cytoskeleton and transport proteins ... randomized insertion persists, and even when inserted into the main 15 locus at chromosome 19, an influence on cellular genes lying nearby, like the MBS85, was reported (Tan et al., 2001) Insertions...

Ngày tải lên: 26/11/2015, 09:54