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... have allowed the synthesis of cell-permeable peptides, as well as the synthesis of cyclic peptides, which are not readily susceptible to intracellular proteolysis and thus are metabolically stable ... parameters of the strand-transfer reaction step Interestingly, despite the fact that the stimulatory INS increased the Km value of the strandtransfer step, as was observed from the shifting of the ... Quantitative determination of IN enzymatic activity was performed with a previously described assay system [75,76] In this assay, the oligonucleotide substrate consists of one oligomer (5Â-ACTGCTAGAGATTTTCCACACTGACTA...

Ngày tải lên: 22/03/2014, 16:21

... operon in Staphylococcus aureus and Staphylococcus xylosus leads to enhanced susceptibility of cells to positively charged antimicrobial peptides In case of Staphylococcus aureus, it was shown that ... substrate selectivity of A-domains can either be accomplished by ATP ⁄ PPi exchange assays or by analysis of the selectivity-conferring residues guided by the nonribosomal code of NRPS A-domains ... mutant when and vancomycin were used simultaneously (Fig 5) Discussion The biosynthesis of d-alanyl-lipoteichoic acid requires four proteins that are encoded by the dlt operon [5] The synthesis starts...

Ngày tải lên: 30/03/2014, 16:20

... men s fear of HIV and AIDS, rooted in hegemonic notions of masculinity, can prevent their wives from adhering successfully to ART Discussion Through our discussion of social constructions of masculinity, ... ART accessible in Zimbabwe This study draws on the perspectives of active ARV users and nurses, focusing on their understandings and retrospective experiences of factors shaping their access and ... that I am HIV positive”, so they even go further by stealing their wives’ hospital cards However, such husbands need counselling (Tsitsi, nurse) Another commonly reported strategy used by husbands...

Ngày tải lên: 20/06/2014, 08:20

... detected by immunofluorescence stance that unspecifically blocks virus activity The possibility of emergence of EF-resistant virus was evaluated by comparing relative H5N1 virus yields in the presence ... extracts first, before adding virus Virus titrations Strain Victoria (H3N2) was titrated by standard plaque assay techniques in MDCK cells with agarose overlays The other strains were assayed by focus ... Following the assays, these supernatants were used to infect another set of cultures under the same conditions as described above This process of sequential infection with supernatants was repeated...

Ngày tải lên: 12/08/2014, 04:20

... consistent, positive outcomes over time with use of this therapy SSA is a self-limited, reversible disease process, which - if treated aggressively at the onset - does not have to be fatal It is ... Children s Healthcare of Atlanta at Egleston successfully demonstrates the use of ECLS as an adjunctive strategy for managing SSA The work there is both important and relevant, because it illustrates ... found small numbers of patients, but it is unknown whether this reflects the small numbers of patients who develop refractory status asthmaticus or just the fact that ECMO is rarely considered,...

Ngày tải lên: 13/08/2014, 16:20

... high-order SMC systems [12], where certain commonalities are observed Euler s Discretization of Single-Input ECB-SMC Systems 2.1 The Single-Input ECB-SMC Systems Without loss of generality, consider ... assumptions each trajectory converges to a period–2 orbit First, let us consider the subsystem y = (x2 , x3 , , xn ) The dynamics of the y subsystem is described by y (k+1) = y (k) + hBc y ... possible Hence, there exists p > such that sp = s0 Now, we show that s1 = s0 implies that s2 = s1 From (11) and the assumptions it follows that s2 = sgn(g(x(2) )) = sgn(g(x(0) ) − αh (s0 ...

Ngày tải lên: 01/07/2015, 10:07

... discussed, some conclusions were drawn, and some suggestions were raised in the thesis Scope of the study The present study investigated “the use of reading strategies when reading ESP by 4thyear ... analysis Chapter presents the results of the study, analyzes the data Part III: Conclusion summarizes the findings, presents the implications and limitations of the study and finally gives some suggestions ... 4thyear English-majored students at the People s Security Academy” The study of learning strategies in other English skills was beyond the scope Organization of the study The study is divided into...

Ngày tải lên: 04/08/2015, 09:41

... discussed, some conclusions were drawn, and some suggestions were raised in the thesis Scope of the study The present study investigated “the use of reading strategies when reading ESP by 4th-year ... interviews This chapter summarizes the main findings based on the findings of the study, discusses the implications and the limitations of the study and proposes some suggestions for further research ... fourth-year English-majored students at the People s Security Academy” is the result of my own research for the degree of Master of Arts and that this thesis has not been submitted anywhere for any...

Ngày tải lên: 25/12/2015, 17:17

... vicious click as soon as you had spoken I suppose it was the Pye receiver being up with profane energy Well, never mind the Pyes As Mrs Rachel says, `Pyes they always were and Pyes they always will ... what Mrs Harmon says worry you," said Diana, with the calm assurance of the four-years matron "Married life has its ups and downs, of course You mustn't expect that everything will always go smoothly ... "Stoutness and slimness seem to be matters of predestination," said Anne "At all events, Mrs Harmon Andrews can't say to you what she said to me when I came home from Summerside, `Well, Anne, you're...

Ngày tải lên: 06/11/2012, 16:13

... naturally unconscious learning occurs through threaded discussions and cooperation when they accomplish tasks Educators must be responsive to today s learners This study illuminates the expanded possibilities ... various points in the task cycle 2.3.4 Task types Many types of second language tasks exist, particularly in the realm of communicative instruction Here is a listing of some key task types found ... analysis to identify target tasks • Classify into target task types • Derive pedagogic tasks • Sequence to form a task-based syllabus • Implement with appropriate methodology and pedagogy • Assess...

Ngày tải lên: 07/11/2012, 15:01

... tendencies The quinone analysis is based on only chemical analysis and does not require any biological and biochemical knowledge or skills Thus, the quinone profiling has high affinity with analysts ... composition used in quinone profiling can achieve a qualitative evaluation of the biomass and biological community structure in one analysis In this study, quinone compositions of microorganisms ... the community structure of the microorganism (Hiraishi, 1989) Isoprenoid quinones are used as coenzymes in the electron transport chain for respiration of microorganisms The major type of quinone...

Ngày tải lên: 05/09/2013, 10:15

... its definition and calculations can be seen in the previous studies by Bejan and Cengel [9-10] Results and discussion 3.1 The analysis of the dehumidifying process of the TED Figure shows a typical ... M.Sc and B.Sc in Engineering Thermophysics from Tianjin University in 2003 and 1998, respectively At present, he is an associate professor of School of Energy and Environment, Hebei University ... completed M.Sc and B.Sc in Engineering Thermophysics from Tianjin University in 1988 and 1985, respectively He is a professor of School of Energy and Environment, Hebei University of Technology Since...

Ngày tải lên: 05/09/2013, 16:10

... that same ID The idea is that your service is supposed to remember this ID and pass it as all the other JLUSs that it may register with as time goes by In that way, there is a single common way ... example, only one JLUS exists and responds Next, the client has the task of using the JLUS to find the desired service To this, the client describes the desired service in any one of several ways The ... be something that implements PrintServiceInterface The second thing we should as part of the Join process is define the set of attributes that our service possesses These could be anything from...

Ngày tải lên: 29/09/2013, 08:20

... that same ID The idea is that your service is supposed to remember this ID and pass it as all the other JLUSs that it may register with as time goes by In that way, there is a single common way ... example, only one JLUS exists and responds Next, the client has the task of using the JLUS to find the desired service To this, the client describes the desired service in any one of several ways The ... be something that implements PrintServiceInterface The second thing we should as part of the Join process is define the set of attributes that our service possesses These could be anything from...

Ngày tải lên: 06/10/2013, 14:20

... that same ID The idea is that your service is supposed to remember this ID and pass it as all the other JLUSs that it may register with as time goes by In that way, there is a single common way ... example, only one JLUS exists and responds Next, the client has the task of using the JLUS to find the desired service To this, the client describes the desired service in any one of several ways The ... be something that implements PrintServiceInterface The second thing we should as part of the Join process is define the set of attributes that our service possesses These could be anything from...

Ngày tải lên: 10/10/2013, 01:20

... this response makes a number of doubtful assumptions First, it assumes that all proponents of ID are religious believers, and this is false: some, such as Michael Denton, are agnostics Besides ... interest of already-established scholars, this will start to happen There are signs that the younger generation of scientists is more open to pursuing ID than previous generations These scientists ... and Design (ISCID) According to its web site,8 ISCID is a cross-disciplinary professional society that investigates complex systems apart from external programmatic constraints like materialism,...

Ngày tải lên: 01/11/2013, 08:20

... this parodic distancing by suggesting that while Sidney shares Gosson s Protestant emphases on profitable service he resists the anticourtly agenda of middle-class Protestantism, and the assertion ... its frustrating impossibility Sidney s description of his own impulse to write poetry strikingly lacks the confident assertion of authority conveyed by the Agrippa story Sidney describes instead ... excess or as the profitless embroidering of the court lady s needle – also represents Indeed, Sidney worries that Pindar himself risked this superfluousness: by praising “highly victories of small...

Ngày tải lên: 01/11/2013, 08:20

... thus also of God) that philosophy was powerless to undermine Hegel called this a thesis about “consciousness.” If we begin with our consciousness of singular objects present to our senses (“sense-certainty,” ... bindingness of any of those projects or basic maxims? It cannot be simply “reason” itself, since that would beg the question of what purposes the use of reason best serves (or whether those purposes ... vassal has the authority to confer that authority on him, since the only authority the vassal possesses is conferred on the vassal by the master himself as author of the law? The master remains...

Ngày tải lên: 01/11/2013, 08:20

... sensibility a priori; and its synthesis of intuitions, conforming as it does to the categories, must be the transcendental synthesis of imagination This synthesis is an action of the understanding ... one substance that necessarily manifests itself to judging agents as a set of causal relationships holding among the various “accidents” of the substance – that is, that skeptical realism and subjective ... really is the way it looks) Indeed, in Hegel s diagnosis, modern post-Cartesian skepticism arises out of taking that “whole” and treating its constituents only as parts, as (in Hegel s sense)...

Ngày tải lên: 01/11/2013, 08:20