role of the extracellular domain

... in the protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the active site in the N2 proteins was independent of the nature of the C-terminal domain, ... in the affinity of the PLRP2 for micellar substrates, and probably allows the interaction of the enzyme with the substrate structure Whether the motion of the lid promotes the recognition of the ... plasmid at the junction between the N-terminal and C-terminal domain sequences of each protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2...

Ngày tải lên: 16/03/2014, 06:20

... of the signal peptide) consists of three domains, an extracellular domain (ECD) (210–220 residues), a membrane-spanning domain, and an intracellular domain [3] The N-terminal ECD of each of the ... confirming the FPLC data, considering that the ‘height’ of the ECD of the AChR is  nm [3] This difference in solubility between the c–e and the b ECDs might be attributed to the primary structure of the ... the ligandbinding domain of nicotinic receptors However, the fact that this protein is most closely related to the a7 subunit of the neuronal AChR (24% identity of amino acids) than each of the...

Ngày tải lên: 30/03/2014, 10:20

... membrane-proximal domains of gp130 is limited to ensure the correct spacing between the CBM and the membrane, each of the domains was replaced by the corresponding domain of the GCSFR The replacement ... coprecipitation experiment maps to domain of the extracellular part of the receptor [28] Therefore, analysis of the ability of the mutants gp130D4 and gp130D4 to form complexes with the LIFR by coprecipitation ... compensate for the shift of the ligand-binding epitopes of the receptors The domain architecture of GCSFR is identical to that of gp130; moreover these receptors share 46% sequence homology The gp130...

Ngày tải lên: 18/03/2014, 01:20

... upstream of the GAF domain are the potential ligands of the Fe-S cluster Consequently, the binding of the ATP analog and the subsequent rearrangement of the interdomain interaction affects the electronic ... electronic condition of the Fe-S cluster through the protein scaffold, resulting in an alteration of the signal rhombicity of the EPR spectrum The divergence of the g-value from that of the whole cell ... approximately the same stability of the variants compared to that of wild-type, confirming that the difference in activity of the variants reflects the intrinsic ability of the variants compared to the transcriptional...

Ngày tải lên: 15/03/2014, 09:20

... that these residues stabilize the distortion of the NAG3 residue In these interactions, we particularly focused on the Trp664 residue, which is located at the bottom of the active site cleft The ... Stereo figures of the model of the bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars and the side-chains of three acidic residues in the conserved ... distortion and twisting of the substrate, so that the glycosidic oxygen faces towards the bottom of the deep cleft A Asp636 NAG1 Enzyme–substrate interactions The crystal structures of the AD2 E526A and...

Ngày tải lên: 15/03/2014, 11:20

... that these inhibitory domain domain interactions are relieved by binding of the metal to the N-terminus of the ATPase To further study the role of the Ccc2 N-terminus in the overall function of the ... [36,37] The current hypothesis involves domain domain interactions between the N-terminus and other domains of the ATPase Indeed, in vitro studies of the purified N-terminus and catalytic loop of the ... about the functionality of the ATPase, they are all interpreted independently of the metallo-chaperone Indeed, studying the Atx1–Ccc2 route in vivo remains challenging, because of the existence of...

Ngày tải lên: 23/03/2014, 05:22

... contains an additional N -domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19] The N-terminal region contains ... labeled The effect of deletions on the overall structure of ClpC1 was studied by CD spectral analysis of the purified proteins in the far-UV region As shown in Fig 3, ClpC1 showed the CD profile of ... to understand the mode of action of these proteins in M tuberculosis In this study, we functionally characterized the ClpC1 protein of M tuberculosis, and investigated the role of its N-terminal...

Ngày tải lên: 23/03/2014, 06:20

... computer model of the HRSV F ECD AD and RS participated in the design of the experiments, oversight of the conduct of the experiments, and AD, RS, and JM participated in the interpretation of the results ... model of the HRSV F protein ECD [35,36] More recently, the complete x-ray structure of the extracellular domain of the F protein of human parainfluenza virus (hPIV3) has been solved [37] The mature ... determine the contribution of the individual cysteine residues in the extracellular domain (ECD) to its functions, a panel of mutations in which each cysteine residue in the ECD of the HRSV F...

Ngày tải lên: 20/06/2014, 01:20

... partner of the C-terminal cytoplasmic domain of the HIV-1 glycoprotein J Virol 2009, 84:1355-65 doi: 10.1186/1742-4690-7-43 Cite this article as: Emerson et al., Role of the C-terminal domain of the ... densitometric quantitation of specific bands on different exposures of the blots to film was carried out using the Image J software from the NIH The amounts of virions released into the respective culture ... excess of target cells, (i.e 1% of the cells in the mixture were infected) (Figs 2C, D) Five hours post-mixing, the CXCR4 antagonist, AMD3100, was added to the mixture and 43 h later, the number of...

Ngày tải lên: 12/08/2014, 23:23

... rafts Truncation of the cytoplasmic domain of the SIV Env glycoprotein alters the conformation of the external domain and results in more stable oligomers of TM glycoprotein [45], and the truncated ... [25] The early steps of HIV and SIV infection include the attachment of viruses to host cells, entry and transport of the genome to the transcription site, formation of the PIC, and import to the ... removal of the cytoplasmic domain can increase the expression of Env on the surface of infected cells, its incorporation into VLPs or membrane vesicles [7-9] and the fusion activity of the Env...

Ngày tải lên: 13/08/2014, 05:22

... level of peri-Golgi RE 5.3 Discussion Chapter 132 Role( s) of the N-terminal extension of VAMP4 in the 137 recycling of the protein 6.1 The recycling of V4nV5-EGFP mutants 137 6.2 The recycling of ... THE ROLE OF THE N-TERMINAL EXTENSION DOMAIN OF VAMP4 IN THE REGULATION OF ITS RECYCLING TO THE TRANS-GOLGI NETWORK TRAN THI TON HOAI INSTITUTE OF MOLECULAR AND CELL BIOLOGY DEPARTMENT OF BIOCHEMISTRY ... required for the transport of secretory proteins from the site of synthesis to the cell surface (Novick et al., 1980) Among these were mutations of SEC18, the gene encoding the yeast homologue of NSF...

Ngày tải lên: 11/09/2015, 09:02

... mediate the translocation of PR domain into the cells To ensure the presence of PR domain within the cells, we transfected HuH7 cells with a plasmid (pcDNA/HisMax/PR) expressing the PR domain The ... described below; and the cell death rate was examined by the trypan blue method after 24 and 48 hr of transfections, respectively Western blot of PR domain The expression of the PR domain was examined ... have not yet been addressed The first question is whether the expression level for RIZ1 varies over the progression of cancer; and the second question is whether the PR domain alone possesses anticancer...

Ngày tải lên: 25/10/2012, 11:15

Ngày tải lên: 25/10/2013, 05:20

... domains with the mid domain (also called the repeat domain) exposed to the extracellular space [10] Thus, it is still not fully clear whether the H pylori T4SS pilus is more similar to that of ... The assembled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu (B) Model-1 for the ... pathogenesis domain is exposed to the extracellular space The repeat domain in the middle of CagY has been shown to be accessible to labelling by antibodies made specifically against this subdomain...

Ngày tải lên: 14/02/2014, 19:20

... reflect the views of The World Bank, its Board of Executive Directors, or the governments they represent The World Bank does not guarantee the accuracy of the data included in this work The boundaries, ... and Professor of Economics at the Wharton School of the University of Pennsylvania Thorsten Beck Professor of Economics and Chairman of the European Banking Center, Tilburg University, Netherlands ... Note that The World Bank does not necessarily own each component of the content included in the work The World Bank therefore does not warrant that the use of the content contained in the work...

Ngày tải lên: 16/02/2014, 10:20

... dominant negative The Hth domain inhibits the activity of a linked AD To further delineate the region required for the inhibitory effect of the Hth domain, we created a series of GDB fusion proteins ... derepressed by deletion of the Hth domain, suggesting that the inhibitory effect of this domain is not specific to the Meis2d AD Comparison of the relative expression levels of these GBD fusion proteins ... around the 5¢-end of exon of Meis2 and Meis3 was tested by RT-PCR The positions of molecular mass markers are shown to the left, and the size in base pairs of the products to the right (the Meis2...

Ngày tải lên: 16/02/2014, 15:20

... to the residue in the midpoint of the respective loop, as shown in Fig To the east of the active site the 37- and 60-loops border the S2¢ pocket of the proteinase The observed differences in the ... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... calculate the Rfree The final R and Rfree values of the model are 0.21 and 0.22 for the complete data ˚ set up to 1.6 A The electron density of the whole main chain of the catalytic domain (B-chain) of...

Ngày tải lên: 19/02/2014, 00:20

... is at pH These preferred activities of the two PPOs of R solanacearum are opposite to the names assigned to them when the genome of this bacterium was sequenced and the function of these conceptual ... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4] Looking at the sequences of the two enzymes, both show absolute conservation of the histidine residues of the CuA ... periods of time by addition of sodium periodate According to the high preference of the enzyme encoded by the RSc0337 gene for the monophenols and the general mechanism for the reaction of tyrosinases...

Ngày tải lên: 19/02/2014, 07:20