... in the protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility ofthe active site in the N2 proteins was independent ofthe nature ofthe C-terminal domain, ... in the affinity ofthe PLRP2 for micellar substrates, and probably allows the interaction ofthe enzyme with the substrate structure Whether the motion ofthe lid promotes the recognition ofthe ... plasmid at the junction between the N-terminal and C-terminal domain sequences of each protein The chimeric protein composed ofthe N-terminal domainof hoPL and ofthe C-terminal domainof hoPLRP2...
... ofthe signal peptide) consists of three domains, an extracellulardomain (ECD) (210–220 residues), a membrane-spanning domain, and an intracellular domain [3] The N-terminal ECD of each ofthe ... confirming the FPLC data, considering that the ‘height’ ofthe ECD ofthe AChR is nm [3] This difference in solubility between the c–e and the b ECDs might be attributed to the primary structure ofthe ... the ligandbinding domainof nicotinic receptors However, the fact that this protein is most closely related to the a7 subunit ofthe neuronal AChR (24% identity of amino acids) than each of the...
... membrane-proximal domains of gp130 is limited to ensure the correct spacing between the CBM and the membrane, each ofthe domains was replaced by the corresponding domainofthe GCSFR The replacement ... coprecipitation experiment maps to domainoftheextracellular part ofthe receptor [28] Therefore, analysis ofthe ability ofthe mutants gp130D4 and gp130D4 to form complexes with the LIFR by coprecipitation ... compensate for the shift ofthe ligand-binding epitopes ofthe receptors Thedomain architecture of GCSFR is identical to that of gp130; moreover these receptors share 46% sequence homology The gp130...
... upstream ofthe GAF domain are the potential ligands ofthe Fe-S cluster Consequently, the binding ofthe ATP analog and the subsequent rearrangement ofthe interdomain interaction affects the electronic ... electronic condition ofthe Fe-S cluster through the protein scaffold, resulting in an alteration ofthe signal rhombicity ofthe EPR spectrum The divergence ofthe g-value from that ofthe whole cell ... approximately the same stability ofthe variants compared to that of wild-type, confirming that the difference in activity ofthe variants reflects the intrinsic ability ofthe variants compared to the transcriptional...
... that these residues stabilize the distortion ofthe NAG3 residue In these interactions, we particularly focused on the Trp664 residue, which is located at the bottom ofthe active site cleft The ... Stereo figures ofthe model ofthe bound substrate in the AD2 E526A mutant (A) and D524A mutant (B) The structures of bound sugars and the side-chains of three acidic residues in the conserved ... distortion and twisting ofthe substrate, so that the glycosidic oxygen faces towards the bottom ofthe deep cleft A Asp636 NAG1 Enzyme–substrate interactions The crystal structures ofthe AD2 E526A and...
... that these inhibitory domaindomain interactions are relieved by binding ofthe metal to the N-terminus ofthe ATPase To further study theroleofthe Ccc2 N-terminus in the overall function ofthe ... [36,37] The current hypothesis involves domaindomain interactions between the N-terminus and other domains ofthe ATPase Indeed, in vitro studies ofthe purified N-terminus and catalytic loop ofthe ... about the functionality ofthe ATPase, they are all interpreted independently ofthe metallo-chaperone Indeed, studying the Atx1–Ccc2 route in vivo remains challenging, because ofthe existence of...
... contains an additional N -domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domainof ClpB [19] The N-terminal region contains ... labeled The effect of deletions on the overall structure of ClpC1 was studied by CD spectral analysis ofthe purified proteins in the far-UV region As shown in Fig 3, ClpC1 showed the CD profile of ... to understand the mode of action of these proteins in M tuberculosis In this study, we functionally characterized the ClpC1 protein of M tuberculosis, and investigated theroleof its N-terminal...
... computer model ofthe HRSV F ECD AD and RS participated in the design ofthe experiments, oversight ofthe conduct ofthe experiments, and AD, RS, and JM participated in the interpretation ofthe results ... model ofthe HRSV F protein ECD [35,36] More recently, the complete x-ray structure oftheextracellulardomainofthe F protein of human parainfluenza virus (hPIV3) has been solved [37] The mature ... determine the contribution ofthe individual cysteine residues in theextracellulardomain (ECD) to its functions, a panel of mutations in which each cysteine residue in the ECD ofthe HRSV F...
... partner ofthe C-terminal cytoplasmic domainofthe HIV-1 glycoprotein J Virol 2009, 84:1355-65 doi: 10.1186/1742-4690-7-43 Cite this article as: Emerson et al., Roleofthe C-terminal domainofthe ... densitometric quantitation of specific bands on different exposures ofthe blots to film was carried out using the Image J software from the NIH The amounts of virions released into the respective culture ... excess of target cells, (i.e 1% ofthe cells in the mixture were infected) (Figs 2C, D) Five hours post-mixing, the CXCR4 antagonist, AMD3100, was added to the mixture and 43 h later, the number of...
... rafts Truncation ofthe cytoplasmic domainofthe SIV Env glycoprotein alters the conformation ofthe external domain and results in more stable oligomers of TM glycoprotein [45], and the truncated ... [25] The early steps of HIV and SIV infection include the attachment of viruses to host cells, entry and transport ofthe genome to the transcription site, formation ofthe PIC, and import to the ... removal ofthe cytoplasmic domain can increase the expression of Env on the surface of infected cells, its incorporation into VLPs or membrane vesicles [7-9] and the fusion activity ofthe Env...
... level of peri-Golgi RE 5.3 Discussion Chapter 132 Role( s) ofthe N-terminal extension of VAMP4 in the 137 recycling ofthe protein 6.1 The recycling of V4nV5-EGFP mutants 137 6.2 The recycling of ... THEROLEOFTHE N-TERMINAL EXTENSION DOMAINOF VAMP4 IN THE REGULATION OF ITS RECYCLING TO THE TRANS-GOLGI NETWORK TRAN THI TON HOAI INSTITUTE OF MOLECULAR AND CELL BIOLOGY DEPARTMENT OF BIOCHEMISTRY ... required for the transport of secretory proteins from the site of synthesis to the cell surface (Novick et al., 1980) Among these were mutations of SEC18, the gene encoding the yeast homologue of NSF...
... mediate the translocation of PR domain into the cells To ensure the presence of PR domain within the cells, we transfected HuH7 cells with a plasmid (pcDNA/HisMax/PR) expressing the PR domainThe ... described below; and the cell death rate was examined by the trypan blue method after 24 and 48 hr of transfections, respectively Western blot of PR domainThe expression ofthe PR domain was examined ... have not yet been addressed The first question is whether the expression level for RIZ1 varies over the progression of cancer; and the second question is whether the PR domain alone possesses anticancer...
... domains with the mid domain (also called the repeat domain) exposed to theextracellular space [10] Thus, it is still not fully clear whether the H pylori T4SS pilus is more similar to that of ... The assembled T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into theextracellular milieu (B) Model-1 for the ... pathogenesis domain is exposed to theextracellular space The repeat domain in the middle of CagY has been shown to be accessible to labelling by antibodies made specifically against this subdomain...
... reflect the views ofThe World Bank, its Board of Executive Directors, or the governments they represent The World Bank does not guarantee the accuracy ofthe data included in this work The boundaries, ... and Professor of Economics at the Wharton School ofthe University of Pennsylvania Thorsten Beck Professor of Economics and Chairman ofthe European Banking Center, Tilburg University, Netherlands ... Note that The World Bank does not necessarily own each component ofthe content included in the work The World Bank therefore does not warrant that the use ofthe content contained in the work...
... dominant negative The Hth domain inhibits the activity of a linked AD To further delineate the region required for the inhibitory effect ofthe Hth domain, we created a series of GDB fusion proteins ... derepressed by deletion ofthe Hth domain, suggesting that the inhibitory effect of this domain is not specific to the Meis2d AD Comparison ofthe relative expression levels of these GBD fusion proteins ... around the 5¢-end of exon of Meis2 and Meis3 was tested by RT-PCR The positions of molecular mass markers are shown to the left, and the size in base pairs ofthe products to the right (the Meis2...
... to the residue in the midpoint ofthe respective loop, as shown in Fig To the east ofthe active site the 37- and 60-loops border the S2¢ pocket ofthe proteinase The observed differences in the ... structure ofthe catalytic domainof DESC1 Scheme Domain organization of human DESC1 membrane region Theextracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... calculate the Rfree The final R and Rfree values ofthe model are 0.21 and 0.22 for the complete data ˚ set up to 1.6 A The electron density ofthe whole main chain ofthe catalytic domain (B-chain) of...
... is at pH These preferred activities ofthe two PPOs of R solanacearum are opposite to the names assigned to them when the genome of this bacterium was sequenced and the function of these conceptual ... 1.10.3.1), but the differentiation between these two types of enzyme is not clear [4] Looking at the sequences ofthe two enzymes, both show absolute conservation ofthe histidine residues ofthe CuA ... periods of time by addition of sodium periodate According to the high preference ofthe enzyme encoded by the RSc0337 gene for the monophenols and the general mechanism for the reaction of tyrosinases...
... To overcome the fact that the prosa z-score is dependant ofthe length ofthe amino acid sequence, the z-score was normalized using the natural logarithm ofthe sequence length [39] The resulting ... structure The distribution ofthe percentage best model, for each ofthe 672 best models, is presented in the left panel Ofthe six template structures used, 54% ofthe sequences give the best ... of PAS-like domains have been solved; the periplasmic ligand-binding domainofthe sensor kinase, CitA [24], and the sensory domainofthe twocomponent fumarate sensor, DcuS [25] These proteins...
... complex at the position ofthe histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex III revealed the presence of these complexes ... and the effect ofthe absence of intact complex I, but the results are too preliminary in this regard Heterologous expression ofthe ESSS-HA subunit The localization ofthe ESSS subunit in the ... sites for cloning into the unique EcoRI and NheI sites ofthe modified pTRIDENT-14neo vectors such that either the HA or the HIS epitope tag was added to the end ofthe ESSS ORF The forward primer...