... of proteasome preparations with cardiolipin and Grx2. The results obtained showed 25% and 65% increased chymotryp- sin-like activity and 61% and 100% increased post- acidic cleavage of n-PT and ... regulatory role in 20S PT activities. We next analyzed Grx2 fragmentation using mass spectrometry, by incubating Grx2 in standard buffer for 30 min or 2 h in the presence of n-PT. After incu- bation, ... is ubiqui- tinated in vivo. Discussion Sulfhydryl groups play a critical role in the function of many proteins, including enzymes, transcription factors and membrane proteins [35]. In a previous...
Ngày tải lên: 23/03/2014, 07:20
... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing MC3T3-E1 ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone. Materials and ... instrumental in stimulating mineralization. Introduction The mechanisms that regulate bone growth and minera- lization remain poorly understood. The cellular events involved in bone formation include...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization" pdf
... Li 4 Abstract Introduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP- 2 on fold increase in abundance of mineralization-related gene expression. Mineralizing MC3T3-E1 ... The increase in cell replication in a miner- alizing cell likely represents a de-differentiation from th e mineralizing phase to the growing phase, and increases expression of GFs most likely induce...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo y học: "Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGF-1 in cultured rat nucleus pulposus cells" ppsx
Ngày tải lên: 09/08/2014, 01:22
Báo cáo y học: " Role of miR-1 and miR-133a in myocardial ischemic postconditioning" pptx
Ngày tải lên: 10/08/2014, 05:21
Báo cáo y học: "The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles" pot
Ngày tải lên: 11/08/2014, 08:21
Báo cáo y học: "Role of contractile prostaglandins and Rho-kinase in growth factor-induced airway smooth muscle contraction" ppt
Ngày tải lên: 12/08/2014, 18:22
Báo cáo y học: " The role of cyclin D2 and p21/waf1 in human T-cell leukemia virus type 1 infected cells" potx
Ngày tải lên: 13/08/2014, 13:20
Báo cáo y học: "The contemporary role of blood products and components used in trauma resuscitation" pdf
Ngày tải lên: 13/08/2014, 23:20
the role of ikkalpha, ikkbeta and nf-kappab in the progression of breast cancer
Ngày tải lên: 22/12/2014, 21:49
Tài liệu The Role of Public Regional Universities in Community and Economic Development doc
... University Regional Learning Alliance – workforce development and training/collaborations with business and industry; meeting the training and education needs of businesses within 60 mile radius in Northern ... Programs and Initiatives Business accelerators and/ or entrepreneurial initiatives Linking teaching and research to business opportunities Collaboration with local business and industry Small Business ... CH/KU County Locations of State System Universities, Branch Campuses and Other Teaching Sites PASSHE in 33 of 67 Counties Presentation Outline Public Purpose of Higher Education The Role of Public Higher...
Ngày tải lên: 21/02/2014, 01:20
The Role Of The United States Postal Service In Public Safety And Security doc
... Security Training. USPS training is aimed at detecting and preventing mail crime, ensuring the security of the mail, and maintaining public safety. e safety and security of the mail at the point ... types of mail crime in particular, suspicious or hazardous incidents involving the mail. Summary Currently, the USPS and IS are doing much in the area of training focused on enhancing public ... The Role of the United States Postal Service in Public Safety and Security Public Safety Education and Awareness 16 e Role of the IS in Public Safety and Security 20 CHAPTER THREE Relaxing the...
Ngày tải lên: 30/03/2014, 13:20
Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
... in uence on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. ... found rotatedtoeachother.TheanglesbetweenplainsofP 3 Â phenyl rings are 98 for OE and SE inhibitors, and 38 for OE and RE inhibitors. The S3Â groups of OE and RE inhibitors having a similar orientation phenyl rings form a similar ... of projections of opposite S d atoms of M et46 and Met146). Ó FEBS 2004 HIV-1 Protease Inhibitors (Eur. J. Biochem. 271) 4457 Inhibitor binding. The inhibitor OE b inds into the p rotease binding...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
... amount of internalized agonist [20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Role of helix 8 and C-termini in bradykinin receptors ... FEBS The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B 1 and B 2 bradykinin receptors Alexander Faussner 1 , Alexandra Bauer 1 , Irina Kalatskaya 1 , ... addition of scintillation fluid. Nonspecific binding was determined in the presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe- cific binding. Internalization of...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Local stability identification and the role of key acidic amino acid residues in staphylococcal nuclease unfolding ppt
... (E129G) and 43.43% (E75G) lower than that of the wild-type protein, suggesting that Glu residues at positions 75 and 129 play key roles in maintaining the native structure of the protein. Furthermore, ... the presence of noncovalent bonding, including an electrostatic bond between E75 and H121, and a hydrogen bond between D77 and T1208. Thus interactions between D77 and E75 and surrounding positively ... Calometric melting curves of wild-type and mutants of SNase. DSC curves of nine proteins (WT, E73G, E75G, E101G, E122G, E129G, D77G, D83G and D95G). Lines 3 and 6 represent E75G and E129G, respectively;...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf
... Nonretained proteins were washed out with 20 m M triethanolamine buffer pH 8.0 and retained proteins were eluted using a gradient of NaCl prepared in the same initial buffer. The presence of the ... clearly increased. Actually, the substitution of R97 by methionine or lysine resulted in the same increment in the pK a of E399 (Table 2). Therefore, M97 and K97 are equally effective in stabilizing ... to E187, in order to minimize unfavourable interactions with the apolar side chain of F331. Thus, the increment in the pK a of E399, in addition to it being closer to E187, may have resulted in a...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... obtained by sequential exposure of cells to increasing concentrations of doxorubicin and was maintained in the presence of 0.5 lm doxorubicin. 2008 parental cells and their MRP1 over-expressing ... phase of fluorescence in the Pgp-over-expressing cells or fluores- cence quenching in wild-type cells (Fig. 5). Inhibition of Pgp with cyclosporine A caused a parallel increase in the amount of TMRM ... aimed to investigate the role played by the leaflets of the plasma membrane in the uptake of drugs into cells and in their extrusion by P-glycoprotein and multidrug resistance-associated protein 1....
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx
... the initial value, but again we observed an increase in light scattering, suggesting Fig. 5. Urea and guanidine treatment of dlFG and sc-dlFG in the presence of bis-ANS. (A) Effects of increasing ... these questions in this work. We sought to determine the role of protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea, GdnHCl and high pressure on the structure and ... unsure of the mechanism of DNA-induced stabilization of the protein, and whe- ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased...
Ngày tải lên: 30/03/2014, 11:20
Leveraging private investment: the role of public sector climate finance pot
... by MDBs include political risk and credit guarantees. These, together with public sector investment in the top level CP3 Fund and the investment grade rating of most public finance institu- tions ... for coordinating devel- oped country finance in this field, and for involving developing countries and the private sector in the co-design of appropriate instruments. ã International investor ... Regulatory and policy risk – reflecting concern about the stability and certainty of the regulatory and policy environment, including the longevity of incentives available for low carbon investment and...
Ngày tải lên: 30/03/2014, 14:20