role of oxidative stress in stem cancer and cancer stem cells

... provides insight into the involvement of oxidative stress in the pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere erosion, and chondrocyte ... Our findings clearly show the presence of oxidative stress in degenerating cartilage, and the resultant telomere erosion and dysfunction of chondrocytes in vitro and in vivo, suggesting a role ... donors and in OA cartilage, suggesting the presence of oxidative damage in ageing and degenerative cartilage These findings provide evidence to support the concept that oxidative stress in articular...

Ngày tải lên: 09/08/2014, 06:22

... about 10% of all cancer incidence and mortality in 20132 Consistent with trends in developed countries, CRC is the most frequent cancer in Singapore, accounting for 14% to 18% of all cancer incidence ... polyploidy or the formation of multinucleated cells6 1 These findings contradict the inhibitory role of STMN1 in cell cycle progression, and allude to possible organ specificity in STMN1 function This ... developing CRC In particular, low fibre diet rich in red meat9 and lack of regular exercise10 appear to correlate closely with CRC incidence Since physical inactivity, obesity, smoking and heavy...

Ngày tải lên: 09/09/2015, 11:25

... protein 42 (Cdc42) and Par polarity complex protein, including Par3, Par6, and atypical protein kinase C (aPKC), may play crucial roles in the generation and controlling direction of polarity in ... filaments and induces growth of a new branch of actin filament at a unique 70 degree angle from the preexisting actin filament Proteins, such as profiling and ADF/cofilin, are also act as regulators in ... regulators in mediating actin polymerization by controling the availability of activated actin monomers, debranching and depolymerizing proteins and capping and severing proteins (Ridley et al.,...

Ngày tải lên: 02/10/2015, 17:15

... resurgence in the incidence of TB occurred in the United States and included increases in the number of TB cases among adults and children and outbreaks of multidrug-resistant TB (MDR-TB) involving ... of skin testing is the Mantoux tuberculin skin test using 0.1 mL of tuberculin units (TU) of purified protein derivative (PPD) (1 ) BCG vaccination contributes to the prevention and control of ... preventing pulmonary TB in adolescents and adults These studies also were not useful in determining a) the efficacy of BCG vaccine in HCWs or b) the effects on efficacy of the vaccine strain administered...

Ngày tải lên: 15/02/2014, 13:20

... word stress, minor word stress and unstressed Thus, in the sentence: “I walked home in the rainstorm” ‗I‘, in , and ‗the‘ are function words and are unstressed; ‗walked‘, ‗home‘ and ‗rainstorm‘ ... to this kind of stress in referring, proclaiming, and reporting utterances Tonic stress is almost always found in a content word in utterance final position Consider the following, in which the ... English speaking competence, the role of sentence stress in enhancing speaking competence Chapter 2: Research methodology Chapter 3: Findings and discussion which consists of three parts: findings,...

Ngày tải lên: 17/03/2014, 16:12

... of the a-aminoadipoyl- side-chain of penicillin N (1), with arginines 74 and 75 apparently playing a less important role in side-chain binding Taken together, the results of this and previous ... consistent with loss of penicillin binding Arginines 306 and 307 Fig Proposed binding mode for penicillin N to the iron(II)-2-oxoglutarate complex of DAOCS, showing possible involvement of selected residues ... productive penicillin (prime) substrate The results in this paper give support to the proposed mode of penicillin binding by DAOCS [1,12] a key feature of which is that arginines 160 and 162 bind to the...

Ngày tải lên: 18/03/2014, 01:20

... following new findings (1) New findings related to theory (a) A new economic equation to relate two levels of sustainable management of economic resource This thesis introduces a new concept of ... combining two types of sustainable management of natural resource in a single economic equation and also under a single diagram as represented by: C ≦ ΔOS*E + S therefore, E ≧ (C – S)/ΔOS1 (b) Introduction ... During late 90s, trade became increasingly criticized by international activists as a source of poverty and environmental degradation in developing countries One of the most important turning...

Ngày tải lên: 20/04/2014, 20:22

... supplements and AD risk, and concluded that use of vitamin E and vitamin C in combination reduced the prevalence and incidence of AD in an elderly population; however, the use of either of these vitamins ... subunit is present in mouse brain in cortex, in hippocampal neurons, and in Purkinje cells [73] Neuronal cells respond to toxic insults including ischemia [73], zinc overload [72], and nerve growth ... global inhibition of src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream...

Ngày tải lên: 19/06/2014, 22:20

... of C22 or C28 affect either cell surface binding or appropriate trafficking of virions during infection Campos and Ozbun observed indistinguishable cell surface binding and uptake into Page of ... http://www.virologyj.com/content/6/1/176 Figure Impact of L2 C22A and C28A point mutations upon vaginal infection of mice by HPV16 pseudovirions Impact of L2 C22A and C28A point mutations upon vaginal infection of mice by HPV16 pseudovirions ... epitope [1,4] This process of revealing the 17-36 epitope Figure Binding of RG-1 antibody to disulphide hairpin of HPV16 L2 Binding of RG-1 antibody to disulphide hairpin of HPV16 L2 An HPV16 L2...

Ngày tải lên: 12/08/2014, 04:20

... mechanism involving COX2 and PGE2 expression in the resolution of inflammation Page of 11 Proinflammatory cytokines, including IL-17 and TNF-a, play a critical role in the progression of synovitis and ... STderived inflammatory cells in the presence of indomethacin Rheumatoid ST contains a number of proinflammatory cytokines that influence osteoclast formation and bone resorption Proinflammatory cytokines ... Effect of interleukin (IL)-17 on pannus-like tissue growth in the presence of indomethacin Synovial tissue (ST)-derived inflammatory cells (n = 7) were incubated with an incremental dose of IL-17 in...

Ngày tải lên: 12/08/2014, 15:22

... combined with vitamin C (Vit C), on training -induced decrease of reduced Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training -induced decrease of reduced ... combined with vitamin C (Vit C), on training -induced increase of lipid Effect of methyl (LPO) levels Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training ... by increases in lipid peroxidation, glutathione oxidation, and oxidative protein damage [28] In our study exercise induced an increase in cellular oxidative stress (as shown by the increase in...

Ngày tải lên: 12/08/2014, 18:22

... alleviating stress by acetylating proteins or toxic metabolites, and exporting endogenously produced toxins from the cells remains to be determined The induction of ppGpp synthesis in liquid cultures of ... Conway and co-workers have also reported a central role for ppGpp in coordinating the global down-regulation of sets of genes involved in active growth in E coli during glucose-lactose diauxie, and ... self-assembling layers of hydrophobic proteins (hydrophobins) encoded by the rodlin (rdl) and chaplin (chp) genes, and subsequently differentiate into chains of unigenomic spores in a process...

Ngày tải lên: 14/08/2014, 08:20

... economic resource This thesis introduces a new concept of combining two types of sustainable management of natural resources in a single economic equation and also under a single diagram It has been ... degradation of the global environmental assets Many efforts are being made by individuals, governments, NGOs and international organizations including Green Peace and WWF International organizations and ... purpose of this thesis is to examine how International Agreements envisage to contributing to conservation of the global environmental assets that are diminishing rapidly at an increasing rate In...

Ngày tải lên: 17/03/2015, 13:26

... sustained increase in [Ca2+]i in PC-12 cells The lysoPI induced increase in [Ca2+]i was attenuated by pretreatment of cells with MBCD and by pre2+ incubation of cells with thapsigargin No change in ... Depletion of cholesterol by pre-incubation of cells with MBCD and depletion of Ca2+ by thapsigargin and incubation in zero external Ca2+ resulted in attenuation of lysoPI induced exocytosis, indicating ... both mRNA and protein expression of sPLA2-IIA were at high levels in the brainstem and spinal cord Moreover, sPLA2-IIA was also localized in the spinal trigeminal nucleus of the brainstem and the...

Ngày tải lên: 10/09/2015, 08:30

... immunolabeling of sPLA2-IIA in the spinal trigeminal nucleus of the brainstem and dorsal horn of the cervical spinal cord is consistent with previous findings of an important role of sPLA2 in nociceptive ... showed bands at 14 kDa of sPLA2-IIA in homogenates from brainstem and spinal cord and a second band at approximately 30 kDa in the spinal cord The second band could be due to dimerization of sPLA2-IIA ... penicillin/streptomycin (Gibco, Invitrogen) The cells were plated in 35 mm Petri dishes, and maintained in an incubator at 37 oC, 100% humidity, with 95% air and 5% CO2 Coverslips containing attached cells...

Ngày tải lên: 10/09/2015, 08:31

... region, indicating the nociceptive function of sPLA2-III The expression profile of sPLA2-III in CNS and its effects on exocytosis in rat PC-12 cells further illustrated the role of sPLA2-III in pain ... mRNA and protein expression were expressed at the highest levels in the brainstem and spinal segments The enzyme was localized to dendrites in spinal trigeminal nucleus, supporting its role in ... of sPLA2 isoforms mRNA and protein expression in rat CNS between previous reports and current findings ……………………… … 116 XI List of Figures LIST OF FIGURES SECTION I Figure.1.1 Site of action of...

Ngày tải lên: 10/09/2015, 08:32

... thapsigargin and recording in zero Ca2+ conditions, or treatment of cells with lanthanum chloride and recording in external solution containing Ca2+, resulted in attenuation of sPLA2-III induced ... levels in the CNS (not visible in this scale) sPLA2-IIA showed greatest expression in the brainstem and spinal cord; conversely, the brainstem and spinal cord contained highest levels of this isoform ... Chapter Role of Group IIA sPLA2 in nociception In contrast to the forebrain, dense labeling for sPLA2-IIA was detected in the brainstem and spinal cord Dense labeling was observed in neurons of the...

Ngày tải lên: 10/09/2015, 08:34

... similar role to NO in wound healing in the rat 1.3 Thesis Overview and Scope The aim of the work presented in this thesis is to determine the role of H2S in skin wound healing and the effect of PAG ... Morphology and physiology in skin wound healing In dermatology, skin is an organ made up of layers of cells (e.g epithelial cells) that protect underlying muscles and organs Interplaying with surroundings, ... Chapter Introduction (2) Investigate the inflammation and wound recovery after PAG administration (3) Compare the H2S releasing profiles and outcomes of S-diclofenac administration and PAG incorporated...

Ngày tải lên: 26/09/2015, 09:39

... overview of the role of PrP in breast cancer metabolism in the study model Picture showing different lactate production in normal and cancer situation Schematic overview of the role of PrP in cancer ... important target of cancer Approximately 50% of all human cancers show defects involving p53, resulting in functional inactivation of its product and subsequent removal of a key component of the DNA ... respectively Breast cancer is the leading cause of cancer mortality in Singaporean females (MOH, 2012) Amongst all the different kinds of cancer, breast cancer is ranked fifth highest in terms of mortality...

Ngày tải lên: 12/10/2015, 17:36

... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [164]) Therefore, inhibition of the, ... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing overreduction of the complexes, favouring the generation of ROS [16,17,22];...

Ngày tải lên: 07/03/2014, 12:20