... enthalpies of binding and stoichiometries were determined from the binding isotherm by fitting a : binding model to the data using the software provided by the manufacturer Results Reaction of fosfomycin ... replacement of R120 by lysine reduces the probability of forming the fully closed form ofthe protein The results obtained with the K22V/R120K double mutant protein point toward a central roleof arginine ... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins However, two other positively charged amino...
... The motif present in DptF is GLDSV, indicating that this putative ACP domain is one ofthe few ACPs in which valine replaces isoleucine (I) or leucine (L) inthe conserved sequence To determine ... carrier proteins (ACPs) [14,15] The genes dptE and dptF are localized immediately upstream ofthe NRPSs of A21987C The resulting proteins DptE and DptF were predicted to be involved inthe lipidation ... Assay Fig In vitro phosphopantetheinylation of apo-DptF and apoAcpK Coomassie blue-stained SDS–PAGE gel (left) and in- gel fluorescence (right) ofthe fluoresceinyl-ACP (+) indicates the reaction...
... ADAM10 and TACE are involved inthe shedding of APP, neither ADAM is involved inthe shedding of ACE Furthermore we show that APMA can distinguish between the shedding of ACE and APP Materials and ... expressing ACE were transfected with the ASOs and then exposed to APMA andthe levels of soluble ACE inthe medium examined (Fig 5) APMA caused a twofold increase inthe shedding of ACE from the ... ADAM10 and TACE play a roleinthe a-secretase shedding of APP, neither protease was responsible for the shedding of ACE APMA stimulates the shedding of ACE but not APP The effect ofthe organomercurial...
... at the start of night prolongation, or 12 wk, an important roleinthe possibility ofthe buds to attain a deeper stage of dormancy Only the 12 wk old plants of northern origin had no flushing ... the chilling requirement to break winter dormancy in Scots pine has been stressed (e.g., Wareing, 1951; Vegis, 1965; Sarvas, 1974) Inthe following,I will use the working definition of dormancy ... (Fig 2) The same was true for the higher temperature 25/15°C compared with 25/5°C Dormancy breaking treatments ofand wk had a dramatic influence, hastening the tilushing rate, especially in the...
... prosthesis inserted into the portal vein andthe inferior vena cava ofthein vivo rabbit model Figure Diagram of silastic H-shaped prosthesis inserted into the portal vein andthe inferior vena cava of ... relating the readings to the wet weight ofthe liver, determined at the end of each experiment The "buffering capacity" ofthe HA was expressed in % as: [Increase in HA flow / Decrease in PV ... occlusion ofthe portal vein for the insertion ofthe mesocaval shunt and data from these preparations have therefore not been included Group The effect of intra-portal ATP Livers from another group of...
... discovery of a brain-specific MT isoform, GIF, in 1991, sparked considerable interest in understanding theroleof all MTs inthe brain, and particularly within theinjured or diseased brain Inthe ... studies examining theroleof GIF in AD, linked to its initial discovery as a factor deficient inthe AD brain There remains no clear consensus on theroleof GIF inthe pathogenesis of AD, and therefore ... conditions [22] Inthe traumatically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regulated by reactive astrocytes inthe vicinity ofthe lesion [23]...
... confirming theroleof endogenous FDX + FDXR in supporting the activity To further confirm theroleof endogenous FDX + FDXR in supporting the catalytic activity, we transformed the FDX (Yah1)- and ... mitochondria-targeting signal inthe yeast system Mitochondrial localization of intact CYP2E1 in yeast cells Because ofthe existing ambiguity inthe literature on the nature and extent of CYP2E1 import into ... Protein targeting to the microsomes requires the cotranslational insertion ofthe newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the...
... important for explaining wilt Inthe case of income, youth living in low-income households experience higher levels of wilt (45%) than youth living in either modest-income (35%) or high-income (23%) ... accumulating school savings may have effects apart from financing school Conley (2001) finds that a doubling of net worth results in an 8.3% increase inthe probability of attending college Further, ... for their youth Youth savings Youth were asked in 2002 whether they had a savings or bank account in their name If they had an account, they were also asked whether they were saving some of this...
... (particularly the short ones) have all zeroes in them In other words, none ofthe bigrams from the training set appears in these reviews This suggests that the main problem with the bigram model ... and E Hovy 2004 Determining the sentiment of opinions In Proc of COLING, pages 1367–1373 M Koppel and J Schler 2005 Using neutral examples for learning polarity In Proc of IJCAI (poster) D Lin ... relation is capturing two different situations, one in which the author himself likes the movie, andinthe other, the author’s cousin likes the movie The overgeneralization resulting from these “stemmed”...
... regions within the bulk of fluid membrane and which are enriched in cholesterol and (glycero-)sphingolipids In addition the fatty acid chains ofthe phospholipids composing these rigid domains are ... than those inthe lipids ofthe surrounding membrane In fact, this fatty acid composition favors a tighter packing of phospholipids and cholesterol, increasing the rigidity of these domains [32–35] ... implications of this subfamily of receptors in cellular signalling make them attractive therapeutic targets The coupling between stimulation ofthe P2X receptor andthe generation of intracellular...
... rolein protein stability [8], and their involvement in interactions with cations offers new perspective inthe analysis ofthe contribution of ions as cofactors in protein folding [38] andin ... those observed for the wild-type, indicating the involvement of Glu53 and Asp287 inthe interaction with the cation (Fig 1E,F) The regain of aromatic chirality at 295 nm andinthe 260270 nm region ... [2] The solid lines through the mutants unfolding data points (lled symbols) are intended to guide the eye ofthe reader and not represent the tting ofthe data Reversibility points are indicated...
... site ofthe N-terminal lobe of human and bovine lactotransferrins Importance ofthe glycan moiety andofthe non–covalent interactions between the N- and C-terminal lobes inthe stability ofthe ... trypsin and chymotrypsin on thein vitro antimicrobial and iron-binding properties of lactoferrin in human milk and bovine colostrum Unusual resistance of human apolactoferrin to proteolytic digestion ... lactoferrin where the protein has to survive the harsh environment ofthe gastrointestinal tract Thus, on the basis of this study, rhLF may be preferred over bLF in oral applications of lactoferrins in...
... facility level in Zambia served inthe capacity of district managers of health programmes The finding that the nurses in Sudan were less involved in planning of activities (15.2% in polio andin measles) ... measles Roles of nurses and midwives in SIAs for polio and measles The involvement ofthe nurses and midwives in activities related to polio and measles SIAs is presented in Table In general, the ... midwives inthe expanded programme on immunization Study objectives The objectives of this study were to document the roles, functions and contribution of nurses and midwives inthe delivery of routine...
... emerging data on the early roleof oligomeric and protofibrilic forms of Aβ in AD is very interesting [25,26] Whether COX-2 and cell cycle proteins are part ofthe molecular mechanisms involved in ... death [13] These in vitro findings indicate that the activation ofmicroglia may play an important roleinthe expression of COX-2 and cell cycle proteins in neurons Post mortem as well as in vivo ... and quantification ofthe immunohistochemical data RV has been involved inthe collection ofthe human post mortem brain material TA, WS, PE and AJMR participated inthe design ofthe study and...
... RA synovium into tissue microcompartments and fluid The finding that the proportion and percentage of chemokine binding sites on macrophages increases inthe inflamed intima of RA and non-RA synovia ... differences inthe percentage of chemokine-positive cells in inflamed regions ofthe RA and non-RA intima However, there were fewer CXCL8 positive cells inthe inflamed RA intima than inthe inflamed ... numbers The binding of CCL2, CCL3, CCL5 and CXCL8 to macrophagesand CXCL8 binding to neutrophils suggests that these receptors may be involved in signaling the migration ofthe phagocytes through the...
... [66] The binding of PTN with syndecan-3 induces neurite outgrowth of embryonic neurons [67] Heparitinase, which cleaves the heparin sulfate chain and disrupts the binding of PTN, inhibits PTN-induced ... tips It binds to the FGFR2b on the epithelial cells and transmits a signal to induce the initiation ofthe lung bud [17-22] Recombinant FGF10 alone can induce budding inthe lung epithelial explants ... and ciliated epithelial cells inthe adult lung [11] HFH-4 induces the expression of βtubulin IV inthe pseudoglandular stage, and promotes the differentiation of ciliated epithelial cells Other...
... polymorphisms in MRL-lpr lupus mice and that enhanced expression of α-actinin may determine the extent of antibody deposition [10] These observations, together with the demonstration that α-actinin immunization ... anti-α-actinin antibodies in lupus patients was associated with a 2.5-fold increase inthe prevalence of LN [14] Finally, levels of anti-α-actinin antibodies correlated with those of anti-DNA ... anti-α-actinin antibodies How can the discrepancies between these disappointing results andthe previous studies be explained? No doubt, a longitudinal design is important, although the number of patients...
... pneumococci inthe blood andin limiting their numbers inthe bloodstream SP-D binds and agglutinates S pneumoniae inthe presence of calcium and is thought to enhance mucociliary and phagocytic ... pneumococci inthe blood of both strains of mice reflected their levels inthe lung These results strongly suggest that lung surfactant protein D plays an important rolein delaying the appearance of ... strains, increased recruitment of neutrophils into inflamed areas of lung tissue was detected within the bronchiolar lumen, inthe bronchiole wall and also inthe perivascular areas within the...
... in my research for their generous time and trust I sincerely thank the Department of Education and Training, the Head ofthe school and teaching staff ofthe school back home in Vietnam for their ... on theroleof feedback (see Mackey, 2007, 2012 for recent reviews), again providing evidence in support ofthe output hypothesis Drawing on the roles of input, interaction, and output, the interaction ... (p.17) In this way, the ZPD is different from the i+1 input in Krashen’s input hypothesis in that the former emphasises theroleofthe learner as agent, and co-construction while the latter the role...
... which, inthe living state in vitro, are continually elongating, retracting, and reorienting themselves, leading to a wide variety of cell shapes (Steinman and Cohn, 1973) Thereafter, the 23 ... enhances the bactericidal activity ofmacrophages during the early phases of infection (Gazzinelli et al., 1994) It also plays an important role by activating bystander resting T cells and maintaining ... recognizing such cells would then release cytotoxic granules resulting inthe killing of 39 Chapter 1: Introduction these infected cells Under these circumstances, the dying cells, CD8 T-cells and other...