role of hypoxia inducible transcription factors in tam function

... to hypoxia [36] It has been reported that hypoxia induces the transcription of HIF-1a mRNA, which increases the level of HIF-1a protein in the presence of continued hypoxia [37,38] Expression of ... of HIF-1a in NPCs under hypoxia (10%) H 18SRNA Bromodeoxyuridine incorporation assay Incorporation of Bromodeoxyuridine (BrdU) into growing (DNA-synthesizing) S phase cells is more accurate in ... arresting the growth of most kinds of cells, promote the proliferation of some types of cells There is increasing evidence that mild hypoxia acts as a potent regulator of various types of stem...

Ngày tải lên: 30/03/2014, 04:20

... Debatin KM: Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance Oncogene 2007, 26:2027-2038 Loor G, Schumacker PT: Role of hypoxia- inducible factor in cell survival during ... the importance of the transcription factor HIF-1 for maintaining the integrity of hypoxic articular cartilage The functional inhibition of HIF-1 coincided with increased apoptosis of articular ... of inflammatory cytokines (a) to (d) Administration of 2ME2 (10 μM) or of dimethyloxaloylglycine (DMOG) (1 mM) did not induce inflammation or fibrosis of synovial tissue A slight thickening of...

Ngày tải lên: 09/08/2014, 13:22

... during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an embryonic cartilaginous limb) of the development of neural crest derivates, including ... target genes other than integrin a10, to clarify the role of AP-2e in chondrocyte differentiation and in the development of osteoarthritis Role of AP-2 in chondrocytic diseases A role for AP-2s as ... factor AP-2 involved in DNA binding J Mol Biol 301, 807–816 Wankhade S, Yu Y, Weinberg J, Tainsky MA & Kannan P (2000) Characterization of the activation domains of AP-2 family transcription factors...

Ngày tải lên: 16/02/2014, 09:20

... (blocked arrows) of different stages in the proposed pathway are indicated Genistein is an inhibitor of tyrosine protein kinase and competitive inhibitor of ATP in other protein kinase reactions; ... Ivan M, Hoffman MA, Kim TY, Huang LE, Pavletich N, Chau V, Kaelin WG: Ubiquitination of hypoxia- inducible factor requires direct binding to the β-domain of the von Hippel-Lindau protein Nat Cell ... nucleus, pull NF-κB off DNA, and export NF-κB back to its resting state in the cytoplasm Thick lines indicate the activating pathway; thin lines indicate the inactivating pathway IκB-independent pathways,...

Ngày tải lên: 12/08/2014, 18:20

... polymerase II, is involved in restoring UV-inhibited transcription and basal transcription Dysfunction of any of these proteins impedes the progression of the NER Following induction of oxidative ... been implicated in human progeroid phenotypes, indicating a dual role of repair factors in genome and telomere maintenance to regulate ageing homeostasis An example of these factors include the helicase ... delayed in development and have reduced expression of matrix proteins resulting in brittle hair and scaly skin Depending on the location of the mutation in either of the proteins, different functions...

Ngày tải lên: 14/09/2015, 08:45

... additional N-domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19] The N-terminal region contains two 32-amino acid ... has an inherent ATPase activity and also functions like a chaperone in vitro Furthermore, we investigated the role of the N-terminal domain of M tuberculosis ClpC1 in its structure and function ... activity in preventing the aggregation of luciferase and reactivating heat-inactivated luciferase Deletion of the N-terminal conserved repeat I (amino acids 1–38) resulted in an alteration in the...

Ngày tải lên: 23/03/2014, 06:20

... signaling pathways involved in cytokine-mediated regulation of hypoxia- induced hypoxia- inducible factor-1α (HIF-1α) translocation and activation Hypoxia and inflammatory signals induce the intracellular ... complex type of involvement of RTP801 in the pathogenesis of ischemic diseases 52 A hypothetical schematic depicting the role of HIF-1 in lung injury is displayed in Fig Figure Hypoxia; Inflammatory ... hemorrhagic shock (HS) initiates an inflammatory response that includes increased expression of inducible nitric oxide synthase and production of prostaglandins [53] Induction of inducible nitric oxide...

Ngày tải lên: 12/08/2014, 19:21

... oxygen-sensitive transcription factors in the regulation of oxidant-mediated α lung injury: role for hypoxia- inducible factor-1α Crit Care 2003, 7 :in press Fein AM, Calalang-Colucci MG Acute lung injury ... the nucleus during hypoxia This process leads to selective upregulation of genes whose products are involved in hypoxia and inflammatory lung injury These include erythropoietin, vascular endothelial ... and lisofylline, a phosphodiesterase inhibitor, are described as being able to reduce proinflammatory cytokines and ameliorate oxidant lung injury in animal models As exciting as this emerging field...

Ngày tải lên: 12/08/2014, 19:21

... activation of the heat shock factor (HSF), a modular protein consisting of a helix–turn– helix class of DNA-binding domain (DBD), a leucine zipper domain, required for trimerization, and a C-terminal transcription ... plethora of stress agents such as cadmium, cerulenin and 1,10-phenanthroline among others, suggesting a role in the response to drug stress Indeed, several target genes encoding a set of proteins involved ... and Yap1 in the C-terminal CRD (residues 570–650 in Yap1 and 330–409 in Yap2) was used to further provide an insight into the function Yap2 Domain swapping of the Yap1 c-CRD by that of Yap2 has...

Ngày tải lên: 30/03/2014, 16:20

... sections were incubated for 30 minutes at room temperature in streptavidin–peroxidase complex (1:500 in DPBS; Dako), rinsed in distilled water and incubated in fast-DAB (3,3'-diaminobenzidine) peroxidase ... medium contained basic medium (as above, but without serum) with × insulin–transferrin– selenium supplement (ITS+1; final concentrations 10 μg/ml bovine insulin, 5.5 μg/ml transferrin, ng/ml sodium ... (green), and 4',6-diamidino-2-phenylindole (blue) Results showed strong staining for CD13, CD29, CD44, CD90 and CD105, weak staining for 3G5, and negative staining for low-affinity nerve growth factor...

Ngày tải lên: 09/08/2014, 10:20

... leads to increasing intracellular stores of GSSG, a potent inhibitor of NF-κB transcription factor DNA binding The pathway leading to the formation of GSH by the action of γ-glutamylcysteine synthetase ... Hemorrhage increases cytokine expression in lung mononuclear cells in mice: involvement of cateκ cholamines in nuclear factor-κB regulation and cytokine expression J Clin Invest 1997, 99:1516-1524 Moine ... the effect of phosphodiesterase inhibitors, believed to play a critical role in modulating the intracellular dynamic ratios of cAMP and cGMP, which are involved in regulating the inflammatory...

Ngày tải lên: 12/08/2014, 19:21

... promoters In G0, proteins appear to bind to the CDE ⁄ CHR, as monitored by in vivo footprinting According to these early experiments all binding is lost in G2 ⁄ M In contrast, constitutive binding ... activating E2Fs cooperate with NF-Y proteins binding to CCAAT-boxes and with Myb proteins associating with a distal Myb site in activating the Cdc2 promoter Binding of the activating E2Fs during ... promoter by in vivo footprinting as being protected in G0 cells The Cdc25C gene is not expressed in resting cells or in cells in G1 phase Only in G2 phase can strong transcription of the gene...

Ngày tải lên: 16/02/2014, 09:20

... regulating protein–protein interactions; hydroxyproline provides a docking site for VHL binding while hydroxyasparagine prevents binding of the coactivator p300 Finally, the long sought after links ... Regulation of gene expression by the hypoxia- inducible factors Mol Interventions 2, 229–243 Wenger, R.H (2002) Cellular adaptation to hypoxia: O2-sensing protein hydroxylases, hypoxia- inducible transcription ... (2000) Role of hypoxia- inducible factor-1 in transcriptional activation of ceruloplasmin by iron deficiency J Biol Chem 275, 21048– 21054 38 Norris, M.L & Millhorn, D.E (1995) Hypoxia- induced protein...

Ngày tải lên: 20/02/2014, 23:20

... MyoD/E-box binding factors The introduction of mutation into one of the putative Etsbinding sequences suppressed the promoter activity In addition, the specific binding of a nuclear protein to the ... the transcription start site) The role of the sequence containing CCT is unknown but it might play a role in the initiation of transcription The active promoter region of the mouse integrin a3 ... aberrant expression of a3b1 integrin in tumor cells and their malignant behavior The increased expression of a3b1 integrin in gastric carcinoma cells is associated with their increased invasion and...

Ngày tải lên: 21/02/2014, 03:20

... Figure Role of hypoxia- regulated HIF transcription factors in RA In the context of RA pathogenesis, hypoxia- induced stabilization of HIF-α protein can potentially modulate genes that are involved in ... SOCS box protein (ASB4), thought to mediate ubiquitination of various target proteins, and the intracellular domain of the Notch receptor (involved in the maintenance of cells in an undifferentiated ... findings are of significant importance to our understanding of the molecular basis of hypoxia tolerance, not only in muscle but also in numerous other diseases (including cancer and RA) and in...

Ngày tải lên: 09/08/2014, 01:22

... catabolic activity, including production of metalloproteinases [31,32] IL-1 also stimulates chondrocyte expression of inducible nitric oxide synthesis, iNOS, which results in an increase in NO production ... stress-induced expression of HIF-1α in chondrocytes are consistent with these findings These findings suggest that HIF-1α may, at least in part, have some role in the pathogenesis of inflammatory arthritis ... degeneration In patients with OA, stronger expression of HIF-1α mRNA in chondrocytes was observed in degenerating regions than in intact regions from the same articular cartilage samples Our findings in...

Ngày tải lên: 09/08/2014, 06:23

Ngày tải lên: 09/08/2014, 07:20

... Figure Role of hypoxia- regulated HIF transcription factors in RA In the context of RA pathogenesis, hypoxia- induced stabilization of HIF-α protein can potentially modulate genes that are involved in ... SOCS box protein (ASB4), thought to mediate ubiquitination of various target proteins, and the intracellular domain of the Notch receptor (involved in the maintenance of cells in an undifferentiated ... findings are of significant importance to our understanding of the molecular basis of hypoxia tolerance, not only in muscle but also in numerous other diseases (including cancer and RA) and in...

Ngày tải lên: 09/08/2014, 13:22

... expression in renal cell carcinoma and its correlation with hypoxia inducible factor-1 alpha BJU Int 2008, 101:480-484 Zhou J, Brune B: Cytokines and hormones in the regulation of hypoxia inducible ... an important role in maintaining intracellular redox equilibrium and in augmenting cellular defenses in oxidative stress [20,21] In above antioxidant response, GSH is converted into glutathione ... evaluation of the effect of salpingectomy on endometrial receptivity in cases of women with communicating hydrosalpinges Hum Reprod 2001, 16:2422-2426 Lin T, Yang MS: Benzo[a]pyrene-induced elevation of...

Ngày tải lên: 10/08/2014, 10:21

... sensitivity as indicated by significantly increase in inhibition of germination in the overexpression line relative to that of wild type (Figure 1C) Increased inhibition of germination in the WRKY60overexpressing ... distinct complexes that may differ in both DNA-binding and transcription- regulating activities The interacting partners of WRKY40 formed during pathogen infection might not be the same as those in ... growing evidence that plant WRKY transcription factors are involved in plant ABA signaling and abiotic stress responses In rice and barley, ABA induces expression of a number of WRKY genes in...

Ngày tải lên: 11/08/2014, 11:21