... provided
further insight into the molecular mechanism driving the
formation ofthe covalent C115–fosfomycin adduct. A
conservative exchange of K22 to arginine maintains fosfo-
mycin binding, while ... in fosfomycin
binding, andin view ofthe multitude of interactions, it was
assumed that deletion of a single interaction would not lead
to a complete loss of fosfomycin binding. Therefore it ... form ofthe protein.
The results obtained with the K22V/R120K double
mutant protein point toward a central roleof arginine 120
in the conformational process occurring during catalysis.
Therefore,...
... domain is one of the
few ACPs in which valine replaces isoleucine (I) or
leucine (L) inthe conserved sequence. To determine
whether DptF is the putative partner of DptE, we
expressed dptF using ... dptF using the pQTev vector in E. coli and
purified the resulting ACP as an N-terminal His7 fusion
protein (Fig. 3) with a yield of 9.5 mgặL
)1
of culture.
The identity ofthe protein was proven ... [37].
Interestingly, inthe case ofthe lipopeptide surfactin,
neither an acyl CoASH ligase-like domain nor an ACP
could be identified within the biosynthetic gene cluster
using bioinformatic tools...
... of PKA inthe mitochondrial targeting of
CYP2E1
The roleof PKA in mitochondrial targeting of
CYP2E1 was investigated using two approaches. The
first approach involved measuring the level of mito-
chondrial ... resulting in
the exposure of cryptic mitochondrial targeting signal,
as inthe case of CYP1A1 [4]; and (b) PKA- or protein
kinase C-mediated phosphorylation of nascent chains
either at the N-terminus ... targeting to the microsomes requires the co-
translational insertion ofthe newly synthesized protein
into the microsomal membrane, where the N-terminal
hydrophobic signal sequence ofthe protein interacts
with...
... accumulating school savings may have effects apart from financing
school.
Conley (2001) finds that a doubling of net worth results in an 8.3% increase inthe probability of
attending college. Further, ... for their youth.
Youth savings. Youth were asked in 2002 whether they had a savings or bank account in their name. If
they had an account, they were also asked whether they were saving some of ... None ofthe
existing research examines the effect of youth savings on college attendance, and only one study
examines the relationship between parent school savings and college attendance.
Theoretical...
... documents and find that the fea-
ture vectors corresponding to some of these docu-
ments (particularly the short ones) have all zeroes
in them. In other words, none ofthe bigrams from
the training ... one in which the
author himself likes the movie, andinthe other,
the author’s cousin likes the movie. The over-
generalization resulting from these “stemmed” re-
lations renders dependency information ... opinions and
identifying the polarity of opinion sentences. In Proc. of
EMNLP, pages 129–136.
618
experiments. First, we train a polarity classifier us-
ing all unigrams from thetraining set....
... (glycero-)sphingolipids. In addition the
fatty acid chains ofthe phospholipids composing
these rigid domains are generally more saturated than
those inthe lipids ofthe surrounding membrane. In
fact, this ... cluster in
response to extracellular stimuli andthe size of the
domains containing those clusters has been estimated.
In summary, multiple lines of evidence have helped to
argue in favour ofthe ... carefully.
Isolation of lipid rafts ⁄ caveolae is very often the first
step inthe biochemical analysis oftheroleof these
domains in cellular signalling. After disruption of cells,
lipid rafts...
... suggested by the lack of coincidence of
the changes in relative fluorescence intensity and in
k
and by the hysteresis ofthe reversibility process
(Fig. 4). At the end ofthe transition, the intrinsic ... that of the
wild-type; however, the integrity ofthe calcium bind-
ing site formed by Asp287 and Glu53 is essential for
interaction with the cation in 7.9 m GdmCl.
An interesting finding ofthe ... observed for the wild-type,
indicating the involvement of Glu53 and Asp287 in the
interaction with the cation (Fig. 1E,F). The regain of
aromatic chirality at 295 nm andinthe 260–270 nm
region for...
... tell us is that inthe past definite men
or groups of men were valuing and acting in a definite way.
Whether they will inthe future value and act inthe same
way remains uncertain. All that can ... advantage in developing new software, selling
Introduction 27
42
For expositions ofthe view ofthe origin and nature ofthe state as a
coercive organization ofthe political means for acquiring income, ... began inthe mid-1960s and culminated inthe inflationary
recession of 1973–1975 by identifying those actions ofthe Fed
with respect to the money supply that initiated and sustained
it.
12
The...
... satisfactory inthe evaluation of
nodal status in this patient group. Since the highly cellular
tissue in reactive lymph nodes may also show increased
intensity, theroleof DWI and ADC in distinguishing
between ... nine
chemotherapy and/ or radiation therapy, ten controls).
Further study using larger numbers of patients is needed
to establish the accuracy of ADC measurement in
monitoring the effect of therapy ... hypointense. Lymph nodes are
noted inthe external iliac region (arrows and arrowhead). The left
anterior and posterior nodes are 13 mm inthe short axis, 20 mm in
the long axis and 6 mm in the...
... concentration ofthe biotinylated Ig remaining
on the cell surface was then quantified by measuring the
binding of [
125
I]Streptavidin. Inthe absence of bTSH, a very
weak internalization ofthe TSHR ... measuring the
binding of [
125
I]Streptavidin. Specific binding at each point was nor-
malized with reference to specific binding before the incubation at
37 °C (0-min point) to derive the fraction of ... expressing the wild-type andthe b1, the
M453T andthe b1-M453T receptors were obtained using
the calcium phosphate precipitation method and main-
tained as described [10]. Internalization of receptor-Ig
complexes...