role of communication and training in the workplace

... provided further insight into the molecular mechanism driving the formation of the covalent C115–fosfomycin adduct. A conservative exchange of K22 to arginine maintains fosfo- mycin binding, while ... in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single interaction would not lead to a complete loss of fosfomycin binding. Therefore it ... form of the protein. The results obtained with the K22V/R120K double mutant protein point toward a central role of arginine 120 in the conformational process occurring during catalysis. Therefore,...

Ngày tải lên: 19/02/2014, 13:20

... domain is one of the few ACPs in which valine replaces isoleucine (I) or leucine (L) in the conserved sequence. To determine whether DptF is the putative partner of DptE, we expressed dptF using ... dptF using the pQTev vector in E. coli and purified the resulting ACP as an N-terminal His7 fusion protein (Fig. 3) with a yield of 9.5 mgặL )1 of culture. The identity of the protein was proven ... [37]. Interestingly, in the case of the lipopeptide surfactin, neither an acyl CoASH ligase-like domain nor an ACP could be identified within the biosynthetic gene cluster using bioinformatic tools...

Ngày tải lên: 07/03/2014, 04:20

Ngày tải lên: 30/03/2014, 14:20

Ngày tải lên: 09/08/2014, 02:21

Ngày tải lên: 13/08/2014, 13:20

... of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. The first approach involved measuring the level of mito- chondrial ... resulting in the exposure of cryptic mitochondrial targeting signal, as in the case of CYP1A1 [4]; and (b) PKA- or protein kinase C-mediated phosphorylation of nascent chains either at the N-terminus ... targeting to the microsomes requires the co- translational insertion of the newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the protein interacts with...

Ngày tải lên: 18/02/2014, 16:20

... accumulating school savings may have effects apart from financing school. Conley (2001) finds that a doubling of net worth results in an 8.3% increase in the probability of attending college. Further, ... for their youth. Youth savings. Youth were asked in 2002 whether they had a savings or bank account in their name. If they had an account, they were also asked whether they were saving some of ... None of the existing research examines the effect of youth savings on college attendance, and only one study examines the relationship between parent school savings and college attendance. Theoretical...

Ngày tải lên: 15/03/2014, 10:20

... documents and find that the fea- ture vectors corresponding to some of these docu- ments (particularly the short ones) have all zeroes in them. In other words, none of the bigrams from the training ... one in which the author himself likes the movie, and in the other, the author’s cousin likes the movie. The over- generalization resulting from these “stemmed” re- lations renders dependency information ... opinions and identifying the polarity of opinion sentences. In Proc. of EMNLP, pages 129–136. 618 experiments. First, we train a polarity classifier us- ing all unigrams from the training set....

Ngày tải lên: 17/03/2014, 04:20

... (glycero-)sphingolipids. In addition the fatty acid chains of the phospholipids composing these rigid domains are generally more saturated than those in the lipids of the surrounding membrane. In fact, this ... cluster in response to extracellular stimuli and the size of the domains containing those clusters has been estimated. In summary, multiple lines of evidence have helped to argue in favour of the ... carefully. Isolation of lipid rafts ⁄ caveolae is very often the first step in the biochemical analysis of the role of these domains in cellular signalling. After disruption of cells, lipid rafts...

Ngày tải lên: 30/03/2014, 02:20

... suggested by the lack of coincidence of the changes in relative fluorescence intensity and in  k and by the hysteresis of the reversibility process (Fig. 4). At the end of the transition, the intrinsic ... that of the wild-type; however, the integrity of the calcium bind- ing site formed by Asp287 and Glu53 is essential for interaction with the cation in 7.9 m GdmCl. An interesting finding of the ... observed for the wild-type, indicating the involvement of Glu53 and Asp287 in the interaction with the cation (Fig. 1E,F). The regain of aromatic chirality at 295 nm and in the 260–270 nm region for...

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 30/03/2014, 13:20

Ngày tải lên: 18/06/2014, 17:20

Ngày tải lên: 22/07/2014, 17:56

Ngày tải lên: 12/08/2014, 11:22

Ngày tải lên: 12/08/2014, 11:22

Ngày tải lên: 12/08/2014, 18:20

Ngày tải lên: 03/03/2015, 09:58

... tell us is that in the past definite men or groups of men were valuing and acting in a definite way. Whether they will in the future value and act in the same way remains uncertain. All that can ... advantage in developing new software, selling Introduction 27 42 For expositions of the view of the origin and nature of the state as a coercive organization of the political means for acquiring income, ... began in the mid-1960s and culminated in the inflationary recession of 1973–1975 by identifying those actions of the Fed with respect to the money supply that initiated and sustained it. 12 The...

Ngày tải lên: 17/01/2014, 02:20

... satisfactory in the evaluation of nodal status in this patient group. Since the highly cellular tissue in reactive lymph nodes may also show increased intensity, the role of DWI and ADC in distinguishing between ... nine chemotherapy and/ or radiation therapy, ten controls). Further study using larger numbers of patients is needed to establish the accuracy of ADC measurement in monitoring the effect of therapy ... hypointense. Lymph nodes are noted in the external iliac region (arrows and arrowhead). The left anterior and posterior nodes are 13 mm in the short axis, 20 mm in the long axis and 6 mm in the...

Ngày tải lên: 13/02/2014, 06:20

... concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In the absence of bTSH, a very weak internalization of the TSHR ... measuring the binding of [ 125 I]Streptavidin. Specific binding at each point was nor- malized with reference to specific binding before the incubation at 37 °C (0-min point) to derive the fraction of ... expressing the wild-type and the b1, the M453T and the b1-M453T receptors were obtained using the calcium phosphate precipitation method and main- tained as described [10]. Internalization of receptor-Ig complexes...

Ngày tải lên: 21/02/2014, 00:20