role of ca 2 in cell regulation

... Figure 32 H&E staining of liver, heart and kidney of 8-week-old 129 SvEvBrdTRIP-Br2 wildtype and mutant mice 22 8 Figure 33 H&E staining of colon, small intestines and lung of 8-week-old 129 SvEvBrd-TRIP-Br2 ... knockdown of TRIP-Br2 is sufficient to inhibit cell- autonomous growth of HCT-116 colorectal cancer cells in vitro Since the loss -of- and gain -of- function of TRIP-Br2 are associated with cell proliferative ... 122 3 .2. 2 TRIP-Br2 overexpression confers anchorage-independent growth in soft agar and promotes tumor growth in athymic nude mice 129 3 .2. 3 TRIP-Br2 is overexpressed in many human cancer cell...

Ngày tải lên: 13/09/2015, 20:18

... conformational change in Bcl -2, exposing its BH3 domain, converting Bcl -2 from anti-apoptotic to pro-apoptotic (Lin, Kolluri et al 20 04) 1.6 Non-canonical role of Bcl -2 in redox regulation: Just as ... species of free oxygen radicals is important in determining the decision of the cell fate A tilt in favor of O2- levels such as the inhibition of Cu/Zn SOD using DDC, with no appreciable increase in ... reaching detrimental levels In our system, we reason that the involvement of SOD in mediating the maintenance of mitochondrial O2- in CEM/Bcl -2 cells during antimycin treatment is at best, minimal...

Ngày tải lên: 14/09/2015, 08:42

... were cultured in DMEM containing 10% fetal bovine serum OP9 cells were grown in aMEM containing 20 % fetal bovine serum C2C 12 cells were maintained in DMEM containing 10% fetal bovine serum Adipogenic ... biologically relevant role of miR -27 in the regulation of adipose tissue functions in vivo, we examined the expression of miR -27 in the genetically obese ob ⁄ ob mice The 23 52 expression levels of ... microscopically with or without hematoxylin staining In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati, OH, USA) constantly maintained...

Ngày tải lên: 18/02/2014, 08:20

... D’Adamio, L 22 23 24 25 26 27 28 29 30 31 32 33 34 35 (20 00) Generation of an apoptotic intracellular peptide by csecretase cleavage of Alzheimer’s amyloid b protein precursor J Alzheimers Dis 2, 28 9–301 ... a major role in determining both the level of expression and the location of the start of transcription of PS1 In the Ets family of transcription Ó FEBS 20 04 Regulation of the presenilin gene ... containing the Drosophila Elk1 binding motif (25 ) (+) or E74 containing a mutated Ets consensus (m) The position of complexes defined in (A) is indicated by arrows or lines Ó FEBS 20 04 Regulation...

Ngày tải lên: 16/03/2014, 18:20

... continuous usage in these experiments was a departure from clinical practice Zhang et al [ 12] reported the critical role of COX -2 in mesenchymal cell differentiation during skeletal repair Using ... osteoblast lineage and is critically involved in bone repair J Clin Invest 20 02, 109:1405-1415 Fitzgerald GA, Patrono C: The coxibs, selective inhibitors of cyclooxygenase -2 N Engl J Med 20 01, 345:433-4 42 ... COX-1-null and COX -2- null mice, they demonstrated the essential role of COX -2 in both endochondral and intramembranous ossification Moreover, the healing of stabilized tibia fractures in COX -2- null mice...

Ngày tải lên: 09/08/2014, 01:21

... COX -2 inhibitor, etodolac, attenuated cough reflex sensitivity to inhaled capsaicin in patients with sinobronchial syndrome This is the first report indicating the modulating role of COX -2 in ... but its expression is markedly induced in inflammation It can be induced by various stimuli, including inflammatory cytokines, resulting in further production of inflammatory substances such as ... COX -2 inhibitors, and cardiovascular risk Drug Safety 20 06, 29 : 621 -6 32 doi:10.1186/1745-9974-6-7 Cite this article as: Ishiura et al.: Role of COX -2 in cough reflex sensitivity to inhaled capsaicin...

Ngày tải lên: 13/08/2014, 10:20

... for cancer Author: Mark Phong Page of 29 1 List of Tables, Figures & Symbols List of Tables S/No Table ID 2. 1 2. 2 2. 3 2. 4 2. 5 2. 6 2. 7 2. 8 2. 9 10 2. 10 11 4.1 12 4 .2 13 4.3 Table 4.3: Genes induced ... Figure 5.9: Effect of Adriamycin and siRNAs Figure 6.1: A new model of p38’s role in DNA damage response 22 0 22 1 22 3 22 6 24 1 Page 13 of 29 1 List of Tables, Figures & Symbols LIST OF SYMBOLS & ABBREVIATIONS ... of Signal Transduction in mammalian cells Figure 1 .2: Canonical overview of the MAPK signaling cascade Figure 1.3: Canonical p38 MAPK signaling pathway: Receptors and signaling cascades leading...

Ngày tải lên: 11/09/2015, 09:02

... 21 2. 6 Definition of AP-1 22 2. 7 Structural organization of c-Jun protein 23 2. 8 Post-translational modifications of c-Jun 24 2. 8.1 Phosphorylation 25 2. 8 .2 Acetylation 25 2. 8.3 Sumoylation 25 ... Cell lines used in this study 42 2 .2 Cell maintenance 42 2.3 Transient transfection of Cos cells (Calcium phosphate method) 42 2.4 Transient transfection using Lipofectamine plus reagent 42 2.5 ... transform cells 27 2. 9.5 c-Jun as a mediator of apoptosis 27 2. 9.6 c-Jun and apoptosis 28 2. 10 Knockout studies on c-Jun 28 2. 11 The Regulation of c-Jun 29 2. 12 The stability of c-Jun protein 30 2. 13...

Ngày tải lên: 16/09/2015, 15:55

... response involving HSP70 21 1.3 Toll like receptor signaling 22 1.4 NFkB 25 1.5 MAPK 26 1.6 Introduction to Annexin-1 (ANXA1) 27 1.6.1 Functions of ANXA1 29 1.6.1.1 ANXA1 in inflammation 29 1.6.1 .2 ... status of the cell (Alldridge and Bryant, 20 03) indicating that ANXA1 plays a major role in the growth function of cells A 40% reduction of ANXA1 levels in the G2/M phase of the cell cycle indicate ... Objectives 39 Chapter 2: Materials and Methods 40 2. 1 List of Reagents used 40 2. 2 Cell Culture 43 2. 2.1 L 929 cell culture 43 2. 2 .2 Bone marrow derived macrophages 43 2. 3 Heat Stress 44 2. 4 Crystal Violet...

Ngày tải lên: 01/10/2015, 17:27

... 28 2. 1 Fish Spawning and Maintenance 28 2. 2 Molecular Biology Techniques 28 2. 2.1 RT-PCR Molecular Cloning 28 2. 2 .2 Polymerase Chain Reaction (PCR) 29 2. 2.3 Agarose Gel Electrophoresis 29 2. 2.4 ... Cultures 32 2 .2. 8 Restriction endonuclease digestion of plasmid DNA 32 2 .2. 9 DNA sequencing 33 2. 2.9.1 PCR Cycle sequencing 33 2. 2.9 .2 Automated sequencing 33 2. 2.9.3 Sequence Analysis 34 2. 3 Analysis ... knockdown in eliciting epiboly defects Synergy of bnip -2 and e-cadherin MO knockdown in eliciting C&E defects Synergy of bnip -2 and e-cadherin MO knockdown in reducing EVL membrane-localised E-cadherin...

Ngày tải lên: 05/10/2015, 19:05

... targets in osteoblast differentiation and matrix mineralization Exp Cell Res 20 00, 25 7 :21 3 -22 2 28 Chen D, Zhao M, Mundy GR: Bone morphogenetic proteins Growth Factors 20 04, 22 :23 3 -24 1 29 Mundy GR: Regulation ... abundance of gene expression in mineralizing cells after 24 hours of treatment As seen in Table 2, we found that BMP -2 treatment caused significant increases in genes associated with mineralization including ... abundance of FGF -2 treated cells (Figure 3) we found that in general, BMP -2 maintained the mineralizing RNA profile of igf-1, alp, and bmp -2 and significantly increased expression of other genes...

Ngày tải lên: 20/06/2014, 04:20

... targets in osteoblast differentiation and matrix mineralization Exp Cell Res 20 00, 25 7 :21 3 -22 2 28 Chen D, Zhao M, Mundy GR: Bone morphogenetic proteins Growth Factors 20 04, 22 :23 3 -24 1 29 Mundy GR: Regulation ... abundance of gene expression in mineralizing cells after 24 hours of treatment As seen in Table 2, we found that BMP -2 treatment caused significant increases in genes associated with mineralization including ... abundance of FGF -2 treated cells (Figure 3) we found that in general, BMP -2 maintained the mineralizing RNA profile of igf-1, alp, and bmp -2 and significantly increased expression of other genes...

Ngày tải lên: 20/06/2014, 07:20

... Choi et al (Eur J Biochem 26 9) 21 22 23 24 25 26 27 roles of insulin and glucagon in the regulation of hepatic glycogen synthesis and turnover in humans J Clin Invest 97, 6 42 648 Petersen, K.F., ... of glycogen in the ADM group is indicative of activated 6-phosphofructo-1-kinase, which can lead to increased 13C labeling at the triose level Such a mechanism can lead to increases in labeling ... that the levels of Fru -2, 6-P2 were Ó FEBS 20 02 significantly increased by adenovirus-mediated overexpression of a mutant form of 6-phosphofructo -2- kinase/ fructose -2, 6-bisphosphatase in the mouse...

Ngày tải lên: 17/03/2014, 17:20

... phosphotyrosine-containing motif and phosphorylated tyrosines in carboxy-terminal region can act as docking sites for SH2-containing signaling molecules PH domain can be involved in membrane localization ... in the A20 B -cell line caused an inhibition of BCR-induced release of IL -2 (Robson et al., 20 04) Mutating four critical carboxyl-terminal tyrosines into phenylalanines abolishes this inhibition ... important role in mediating signaling cascades Reports pertaining to the studies of Dok-1 and were mainly explored in cells of hematopoietic origin for the following reasons Hematopoietic cells, in...

Ngày tải lên: 14/09/2015, 09:08

... 27 5 (20 08) 3815–3 826 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 3 825 Role of CerK in PPARb-induced keratinocyte survival 15 16 17 18 19 20 21 22 23 24 25 26 K Tsuji et al regulate ABCA 12 ... rate of increase in CerK activity in cells treated with LD was larger in cells FEBS Journal 27 5 (20 08) 3815–3 826 ª 20 08 The Authors Journal compilation ª 20 08 FEBS K Tsuji et al Role of CerK in ... compilation ª 20 08 FEBS 3 823 Role of CerK in PPARb-induced keratinocyte survival K Tsuji et al solvent system Quantification of bands was carried out using the Imaging Analyzer BAS2000 Measurement of total...

Ngày tải lên: 18/02/2014, 18:20

... Journal 27 5 (20 08) 29 42 29 55 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 29 43 Cysteinyl-based modification of the 20 S proteasome G M Silva et al from cells grown in glycerol ⁄ ethanol-containing ... Chem 27 3, 3 92 397 Rodrı´ guez-Manzaneque MT, Ros J, Cabiscol E, Sorribas A & Herrero E (1999) Grx5 glutaredoxin plays a central role in protection against protein oxidative 29 54 19 20 21 22 23 24 ... degradation of Grx2 in the presence of cardiolipin (CDL) 20 % SDS–PAGE representative of n-PT preparations (2. 5 lg) incubated for h at 37 °C in standard buffer with Grx2 (5 lg) Lane 1, purified Grx2 incubated...

Ngày tải lên: 23/03/2014, 07:20

... centrifuged Ca2 + and Pi were determined in the pellets corresponding to the mineral structure Symbols corresponding to various Ca2 + concentrations are: n, Ca2 + 2. 5 mM; e, Ca2 + mM; h, Ca2 + 7.5 mM; s, Ca2 + ... University of Lyon I, Villeurbanne, France Results Evidence of different steps in the GPI-bALP mediated mineralization: effects of Ca2 + concentration To assess the role of Ca2 + in initiating the mineralization ... When 2. 5 mM Ca2 + was added in the incubation medium, no calcium-phosphate minerals were obtained during 72 h of incubation The Ca/ Pi ratio was 0.8 at 144 h, characteristic of a poorly crystalline...

Ngày tải lên: 23/03/2014, 17:21

... unsubstituted catechol and to 3-DMC is found (Table 1) The region facing positions and of the catechol ring is mainly formed by a loop containing residues 24 6 24 9 in C2,3O (24 0 24 3 in DHBD and 24 8 25 1 in ... corresponding to subsite 2 is larger and contains two arginine residues FEBS Journal 27 3 (20 06) 29 63 29 76 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 29 65 Thr249 in catechol -2, 3-dioxygenase ... 0 .2 0.5% of that measured on catechol (180 s)1) Therefore, the low reactivity of FEBS Journal 27 3 (20 06) 29 63 29 76 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 29 67 Thr249 in catechol -2, 3-dioxygenase...

Ngày tải lên: 30/03/2014, 10:20

... metformin 16 Role of the Adipocyte in Development of Type Diabetes effects (Tang et al., 20 01), acting as co-adjuvants in the reduction of the incident of diabetes (Doggrell, 20 02) , increasing vascular ... chain isoforms in single fibers from m vastus lateralis of sprinters: influence of training” Acta Physiologica Scandinavica Vol 151, No (June 1994), pp 135- 42 Andrade-Rodríguez HJ, Valadez-Castillo ... Loskutoff D (20 03) Monocyte chemoattractant protein in obesity and insulin resistance Proceedings of the National Academy of Sciences of the United States of America Vol 100, No 12 (June 20 03),...

Ngày tải lên: 27/06/2014, 19:20

... increase in the phosphorylation of cdc2 occurred at 12 h, indicating cell cycle arrest at the G2 checkpoint, and declined to the initial level at 48 h Cyclin B1 that forms the cdc2/cyclin B1 complex ... p53 gene somatic mutations in human tumors and cell lines: updated compilation and future prospects Nucleic Acids Res 1997, 25 :151-157 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 ... K: Comparative analysis of G2 arrest after irradiation with 75 keV carbon-ion beams and 137Cs γ-rays in a human lymphoblastoid cell line Cancer Detect Prev 20 03, 27 :22 2 -22 8 Spitz FR, Nguyen D,...

Ngày tải lên: 09/08/2014, 10:20