role of brca1 in cell cycle control

... make up the cell cycle are critical to maintain the integrity of the cell, particularly of its genetic contents Negative regulatory barriers in the form of inhibitory proteins are inserted at ... transcription late in the cell cycle, largely accounting for the active window of APCCdc20 (Prinz et al 1998), APCCdh1 is expressed constitutively throughout the cell cycle (Weinstein 1997; Prinz et al ... ubiquitin-proteasome pathway in cell cycle control 155 Fig The spindle assembly checkpoint Unattached kinetochores establish an inhibitory complex consisting of Bub3, Mad3/BubR1 and Mad2, that binds...

Ngày tải lên: 25/10/2013, 21:20

... targets of miR-210 59 4.5 Screening of predicted targets of miR-193a 63 5. Discussion 67 5.1 Differential expression of miRNAs during cell cycle phases 67 5.2 Role of miR-210 in cell cycle 69 5.3 ... various miRNAs in cell cycle regulation, using hepatocellular carcinoma cell lines. This will help to identify specific miRNAs that oscillate during the cell cycle phases. These cell lines will also ... genetic loci involved in the control of developmental timing in C. elegans uncovered a ~22nucleotide single-stranded non-coding RNA as the product of the lin-4 gene. Lin-4 RNA repressed the protein levels...

Ngày tải lên: 05/10/2015, 22:31

... targets of miR-210 59 4.5 Screening of predicted targets of miR-193a 63 5. Discussion 67 5.1 Differential expression of miRNAs during cell cycle phases 67 5.2 Role of miR-210 in cell cycle 69 5.3 ... various miRNAs in cell cycle regulation, using hepatocellular carcinoma cell lines. This will help to identify specific miRNAs that oscillate during the cell cycle phases. These cell lines will also ... genetic loci involved in the control of developmental timing in C. elegans uncovered a ~22nucleotide single-stranded non-coding RNA as the product of the lin-4 gene. Lin-4 RNA repressed the protein levels...

Ngày tải lên: 06/10/2015, 20:35

... extracellular lipoproteins, recycles within the cell in extra-lysosomal, weakly acidified recycling endosomes which are rich in FC, sphingolipids and caveolin, the major structural protein of cell ... cell lineages, are in fact virtually devoid of macrophages (Wood et al., 2000) The expression of ABCA1 in cells of myeloid lineage is unequivocal It has indeed been assessed in several cell lines ... and C-terminal extracellular loops; red, nucleotide-binding domains; orange, intervening domain with putative regulatory function The position of the starting methionine (ATG) and of the stop...

Ngày tải lên: 29/12/2017, 16:40

... area of great interest, as loss of genomic integrity is often implicated in tumorigenic and oncogeneic events Critical to the ability of healthy cells in maintaining genomic integrity are the cell ... pathway, in DNA damage-induced G2 checkpoint control While examining the role of p38 in the G2 checkpoint pathway, we found that inhibition of p38 by biochemical or siRNA was unable to affect G2 cell ... establishment and maintenance of a DNA damage-induced cell cycle arrest in G2 The ability of cancer cells to establish a cell cycle arrest in response to genotoxic agents is one of the reasons for...

Ngày tải lên: 11/09/2015, 09:02

... regulate cell cycle progression: an issue of quality control assurance 37 1.1.5 Dysregulation of the cell cycle in human cancer 41 1.1.5.1 Role of altered cyclin-dependent kinase (CDK) ... 1.1 Current paradigm of the cell cycle and cancer 25 1.1.1 The general schema of cell cycle progression: simplifying complexity 25 1.1.2 The control of cell cycle progression ... proteins 58 1.2.6 Other interactors of TRIP-Br proteins 61 1.3 Specific aims of this study 62 1.3.1 Specific Aim 1: To unravel the physiologic role( s) of TRIP-Br2 in cell cycle...

Ngày tải lên: 13/09/2015, 20:18

... probable role of ERRβ in the regulation of cell cycle by regulating p18, p21cip and cyclin D1 in breast cancer cells Furthermore, fluorescence-activated cell sorting analysis for cell cycle showed increase ... facilitates binding of ERRβ on half EREs through ERα a ChIP assay showing ERα binding to half EREs on ERRβ promoter in- vivo in the presence of estrogen in (i) MCF7 and (ii) T47D cells b ERRβ binding in ... transition in cell cycle through binding to PCNA in P53 deficient cells [65] p18 belongs to INK4 family and can inhibit cyclin dependent kinases potentially Reduced levels of p18 were detected in hepatocellular...

Ngày tải lên: 24/07/2020, 01:21

... expression of an E2F variant containing the DNA binding motif but not the pRb-binding or transactivation domain or introduction of a competitor plasmid containing multiple E2F binding sites, preventing ... for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins Mdm2 (as well as c-Abl) binds to the C-domain All pocket proteins ... 14:2358–2365 Weinberg RA (1995) The retinoblastoma protein and cell cycle control Cell 81:323–330 Welch PJ, Wang JY (1993) A C-terminal protein-binding domain in the retinoblastoma protein regulates...

Ngày tải lên: 25/10/2013, 21:20

... understanding of systemic properties (such as the emergent RP in the mammalian cell cycle) in terms of the multitude of reaction steps and interactions between the cell? ??s molecular parts (e.g cell cycle ... occurrence of RP (as a fraction of total cell cycle time) upon a 1% change in activity of a reaction step A positive control of a reaction step indicates that an increase in the activity of the step ... description of regulation of the G1-S transition of the mammalian cell cycle Biotechnol Bioeng 65, 631–637 Aguda B & Tang Y (1999) The kinetic origins of the restriction point in the mammalian cell cycle...

Ngày tải lên: 15/03/2014, 09:20

... Table 1) of which the mRNA levels were mainly high in G2/M cells (Figure 4b) WEE1/MYT1/MIK1 kinases Figure Cyclin-dependent kinase cyclin-binding motifs Alignment of the cyclin-binding motifs of all ... Histogram indicating the proportion of cells in a certain cell cycle phase and chloroplast conformation during the cell cycle Divided chloroplasts were observed starting from h after illumination, ... cyclins that probably transduce the environmental status of the cells to the cell cycle machinery Page of 19 Results and discussion Annotation of the cell cycle genes in diatoms The following cell...

Ngày tải lên: 09/08/2014, 20:21

... 9 of 11 Additional file 16: A figure showing Hoechst nuclear staining of dividing cdc28-ts mutant cells. Control data displaying synchronous division of the yeast cells along with the cell cycle ... expression during the budding yeast cell cycle. It reveals periodic expression of both protein-coding and non-coding RNA and profiles the expression of non-annotated RNAs throughout the cell cycle for ... http://genomebiology.com/2010/11/3/R24 Page 5 of 11 progression observed for protein-coding genes [38]. To elucidate the role of these intergenic transcripts in cell cycle regulation, deletion strains for 10 of the 11 unan-...

Ngày tải lên: 09/08/2014, 20:21

... The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures Maria Torvinen, Hinnah Campwala and Iain ... cytokines Th1 cells are mainly involved in cell- mediated inflammatory reactions and in development of chronic inflammatory conditions, whereas Th2 cells enhance antibody production by B cells ... regulation of the IP-10 secretion The aim was to investigate the pathways of IP10 secretion in a in vitro system including the cell types most likely involved in the IP-10 secretion in the lung...

Ngày tải lên: 12/08/2014, 15:21

... part of the great lab team iv Abstract Krzysztof Kamocki THE ROLE OF CERAMIDES IN CIGARETTE SMOKE-INDUCED ALVEOLAR CELL DEATH The complex pathogenesis of emphysema involves disappearance of alveolar ... reduce lung cell apoptosis CS increased ceramides in the whole lung and in cultured primary structural lung cells Exposure to CS activated within minutes the acid sphingomyelinase, and within weeks ... of RTP801 inhibited only epithelial, but not endothelial cell apoptosis induced by ceramide In conclusion, CS induces acid sphingomyelinase-mediated ceramide upregulation and apoptosis in a cellspecific...

Ngày tải lên: 24/08/2014, 09:42

... Isolation of the Ect2 proto-oncogene 37 2.2 Cell cycle- dependent regulation of Ect2 37 2.3 N-terminal domains of Ect2 are similar to cell cycle regulatory proteins 41 2.4 Cellular functions of Ect2 ... scheme of Ras and Rho signaling events cumulating in cellular transformation and tumourigenesis Figure 2.1 Structure of Ect2 gene Figure 2.2 Alignment of Ect2 BRCT domains with other BRCT-containing ... of Ect2 in U118 glioma cells Ect2 was found in both cytoplasmic and nuclear fractions during interphase (Fig 8a) Surprisingly, Ect2 was found in the chromatin-bound fraction, indicating either...

Ngày tải lên: 11/09/2015, 16:04

... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... what kinds of cells those human embryonic stem cells were differentiated into, or whether SPHK inhibition may aid the stem cells to differentiate into a specific cell- type(s) In Chapter I introduced ... staining in each sample was quantified by dissolving the staining with 100% of isopropanol and detecting the absorbance at 500nm 3.1.3.3 Fluorescence Immunostaining Fluorescence immunostaining...

Ngày tải lên: 11/09/2015, 16:06

... Chapter Role of S1P in human BM- and AD- MSCs proliferation CHAPTER ROLE OF S1P IN HUMAN BM- AND AD- MSCS PROLIFERATION In Chapter 3, I discussed the regulatory effects of SPHK inhibitors in human ... have proliferative functions, promoting cell growth in various types of cells (Zhang et al., 1991; Olivera and Spiegel, 1993), including in embryonic stem cells (Pébay et al 2005; Harada et al ... cells were incubated on ice for 20 min, followed by two washes in 2% FCS/PBS, before resuspension in 100 μl of 4% paraformaldehyde and incubation on ice for 20 Cells were again washed twice in 2%...

Ngày tải lên: 11/09/2015, 16:06

... (1999) "Role of sphingosine 1-phosphate in the mitogenesis induced by oxidized low density lipoprotein in smooth muscle cells via activation of sphingomyelinase, ceramidase, and sphingosine kinase." ... et al (1998) “N,N-Dimethylsphingosine Is a Potent Competitive Inhibitor of Sphingosine Kinase but Not of Protein Kinase C: Modulation of Cellular Levels of Sphingosine 1-Phosphate and Ceramide.” ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... rheostat of maintaining proliferation/differentiation in MSCs is altered by SPHK inhibitors, cell fate would be switched into cell differentiation or cell death Through mediating the concentration of ... reported to regulate the role of S1P in heart cell migration during embryogenesis in zebrafish (Ishii et al., 2002), indicating its function in cardiovascular system Interestingly, while S1PR1 has ... immunosuppressive effects of S1P by inhibiting proliferation and secretion of cytokines, while enhancing secretion of the suppressive cytokine Interleukin-10 (IL-10) S1PR5 is the most recent member in S1PRs family...

Ngày tải lên: 11/09/2015, 16:06

... (b) Bán kính cell nửa cell ban đầu, dung lượng tăng lên Bằng kỹ thuật sector hóa cell vô hướng thành cell nhỏ với cell 120 thành cell cell cell 60 chứng minh kỹ thuật sector hóa cell làm tỷ lệ ... Mobile Switching Center Nondeterminitistic Polynomial Non-Line -Of- sight Non-Dominated Sorting Genetic Algorithm Probability density function Single Objective Optimization Problem Vũ Hoàng Linh - D04VT2 ... phát cell p t công suất phát ban đầu cell ban đầu Một kỹ thuật khác làm tăng dung lượng cell mà giữ cho bán kính cell không thay đổi kỹ thuật chia cell thành cell hình dẻ quạt, gọi sector hóa cell...

Ngày tải lên: 30/04/2014, 23:10