restriction fragment length polymorphism

Báo cáo khoa học: " Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP)" pdf

Ngày tải lên : 07/08/2014, 18:20

... 332 Sandeep Kumar Singh et al of M tuberculosis and M bovis Restriction fragment length polymorphism (RFLP) analysis based on IS6110 and IS1081 sequences easily and rapidly ... 1998, 4, 59-70 Das S, Paramasivan CN, Lowrie DB, Prabhakar R, Narayanan PR IS6110 restriction fragment length polymorphism typing of clinical isolates of Mycobacterium tuberculosis from patients ... Narayanan S, Sahadevan R, Narayanan PR, Krishnamurthy PV, Paramasivam CN, Prabhakar R Restriction fragment length polymorphism of M tuberculosis strains from various regions of India, using direct...

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Báo cáo khoa học: "Oak chloroplast-DNA polymorphisms detected by restriction fragment length polymorphism (RFLP)" doc

Ngày tải lên : 08/08/2014, 19:21

... kb), while a 2.5-kb restriction fragment Probe P8, one of the fragments adja- cent to P6 in Petunia, detected a BglII fragment similar to that of P6 in Q petraea samples (5-kb fragment) However, ... did not show polymorphisms with the restriction enzymes used The polymorphism detected by the P6 Petunia probe with BglII had been described earlier (Kreike et al, 1991) The other polymorphisms ... smaller polymorphic fragment found in Q robur samples (3.6-kb fragment) was not detected (fig C) CONCLUSIONS we described polysites within the oak cpDNA Four morphic of these polymorphisms were...

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Báo cáo khoa học: " IS900 Restriction Fragment Length Polymorphism (RFLP) Analysis of Mycobacterium avium subsp. paratuberculosis Isolates from Goats and Cattle in Norway" pdf

Ngày tải lên : 12/08/2014, 15:21

... Molecular epidemiology of Mycobacterium avium subsp paratuberculosis: IS900 restriction fragment length polymorphism and IS1311 polymorphism analyses of isolates from animals and a human in Australia ... Mycobacterium avium subsp paratuberculosis isolates from goats and cattle 15 Figure IS900 restriction fragment length polymorphism patterns detected with the enzymes BstEII and PstI in Mycobacterium avium ... detection of the insertion segment IS900 by PCR (Sigurðardóttir et al 1999) IS900 restriction fragment length polymorphism analysis (RFLP) RFLP analysis was performed as described by Pavlik et...

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báo cáo khoa học: " Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism" pps

Ngày tải lên : 11/08/2014, 11:21

... plays a negative role in the accumulation of BaMV [23,24] This study used cDNA-amplified fragment length polymorphism (AFLP) to identify differentially expressed genes during BaMV infection in ... microarray data is unavailable [25] This method employs two restriction enzymes to generate short fragments in the analysis of AFLP The choice of restriction enzyme depends on the complexity of the target ... genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism BMC Plant Biology 2010 10:286 ...

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Báo cáo kỹ thuật di truyền kỹ thuật aflp (amplified fragments length polymorphism)

Ngày tải lên : 18/12/2014, 13:15

... 1990, công ty Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp-amplified -fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html ... // -G MseI AATTC // T // -CTTAA G // AAT recognition sequences TAA // T // DNA restriction Chạy PCR với lọai mồi chọn lọc, mồi cấu tạo gồm phần: Trình tự nòng cốt( CORE), trình...

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báo cáo kỹ thuật di truyền- kỹ thuật aflp (amplified fragments length polymorphism)

Ngày tải lên : 06/01/2015, 21:50

... 1990, công ty Keygene phát triển kỹ thuật AFLP ( Amplified Fragments Length Polymorphism) AFLP kết hợp RFLP (Restriction Fragments Length Polymorphism) PCR( polymerase chain reaction) đa hình đọan ... http://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/TechAFLP.shtml http://doc.edu.vn/tai-lieu/de-tai-chi-thi-phan-tu-ky-thuat-aflp-amplified -fragment- lengthpolymorphisms-61035/ http://idoc.vn/tai-lieu/chi-thi-phan-tu-ky-thuat-aflp.html http://tailieu.vn/doc/ky-thuat-aflp-348752.html...

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Bài báo cáo môn di truyền phân tử kỹ thuật AFLP (amplified fragments length polymorphism)

Ngày tải lên : 21/07/2015, 15:36

... biến đổi) chọn lọc dựa kiểu phép chọn lọc truyền thống thực Bằng kỹ thuật AFLP (ampliﬁed fragment length polymorphism) , phương pháp có hiệu để nhân dòng giải trình tự marker liên kết để xác định ... vụ lợi ích cho người Nguyên tắc Nguyên tắc phương pháp AFLP dựa độ đặc hiệu enzym cắt giới hạn (restriction enzym – RE) vị trí nhận biết chúng DNA gen Sự khác biệt vị trí hai cá thể tạo phân đoạn ... sử dụng lượng nhỏ DNA ban đầu (2.5pg-25ng) - Khuếch đại có chọn lọc lượng lớn đoạn DNA đa hình (polymorphism) phản ứng PCR, phù hợp cho việc phân tích đa dạng quần thể có quan hệ gần - Không cần...

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Báo cáo y học: " Restriction Site Tiling Analysis: accurate discovery and quantitative genotyping of genome-wide polymorphisms using nucleotide arrays" pptx

Ngày tải lên : 09/08/2014, 20:21

... array-based polymorphism discovery and genotyping assays Here we present a generally applicable technique, Restriction Site Tiling Analysis (RSTA), which scans for restriction cut site polymorphisms ... sample and restriction digest half Label fragments with red or green Combine and hybridize to arrays Cut site Mutation Microarray feature Figure Restriction site tiling analysis identifies polymorphisms ... high-throughput polymorphism detection approaches Parameter SFP RAD tagging RAD sequencing RSTA Marker type SNPs and indels Restriction cut site polymorphisms Sequence data: SNPs next to restriction...

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Bơm ECD-V - P - Intake Restriction Control

Ngày tải lên : 23/10/2012, 09:09

... Energized (ON) E Port Not Energized 1-4 To Chamber A or Chamber B Diaphragm Port Air Port F Port Intake Restriction Position and Driving Conditions The energization of VSV1 or VSV2 is selected according...

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Báo cáo y học: "Translational Medicine and Reliability of Single-Nucleotide Polymorphism Studies: Can We Believe in SNP Reports or Not"

Ngày tải lên : 25/10/2012, 10:51

... (57.5) 1-3 polymorphisms tested 13 (48) 14 (52) > polymorphisms tested (7) 25 (93) 1-3 polymorphisms tested (19) 17 (81) > polymorphisms tested (24) 19 (76) 1-3 polymorphisms tested > polymorphisms ... according to number of polymorphisms tested Polymorphisms No of studies (%) P-value Positive outcome (%) Negative outcome (%) 1-3 polymorphisms tested 29 (74) 10 (26) > polymorphisms tested 17 ... studies analysing a determined polymorphisms between the studies examining it within 1-3 polymorphisms versus studies examining the same polymorphism among more than polymorphism Since the distribution...

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Báo cáo y học: "The Impact of a Nationwide Antibiotic Restriction Program on Antibiotic Usage and Resistance against Nosocomial Pathogens in Turkey"

Ngày tải lên : 25/10/2012, 11:00

... study to evaluate the effect of a nationwide restriction programme on both antibiotic consumption and antimicrobial resistance rates Few hospitals had a restriction policy before 2003 in Turkey Five ... drugs The restriction policy has resulted in clear and immediate saving The long term influence on medical budget may be stronger than the beginning The financial impact of antimicrobial restriction ... following a restriction policy in a Greek tertiary care hospital Int J Antimicrob Agents 2007; 29(1):34-38 Regal RE, DePestel DD, VandenBussche HL The effect of an antimicrobial restriction program...

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Báo cáo y học: "Genetic polymorphism of p53, but not GSTP1, is association with susceptibility to esophageal cancer risk – A Meta-Analysis"

Ngày tải lên : 25/10/2012, 11:40

... nucleotide polymorphisms in these genes might render the carrier susceptible to cancer, including esophageal cancer It was reported that the p53 gene is polymorphic and among its single nucleotide polymorphisms, ... have reported that the p53 codon 72 polymorphism may be associated with tumor susceptibility to a variety of cancers recently.[16-18] The GSTP1 gene displays a polymorphism, an A>G change at codon ... reports (last search update February 2010, using the search terms “p53”, “polymorphisms” and “esophageal cancer”; “GSTP1”, “polymorphisms” and “esophageal cancer”) Additional studies were identified...

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Báo cáo y học: "Association between regulated upon activation, normal T cells expressed and secreted (RANTES) -28C/G polymorphism and asthma risk – A Meta-Analysis"

Ngày tải lên : 26/10/2012, 09:39

... frequent -28C, suggesting that the polymorphism can increase RANTES expression in the human body [12] Therefore, it’s reasonable to hypothesize that the RANTES -28C/G polymorphism may functionally ... reports (last search update February 2009, using the search terms “RANTES”, “polymorphisms” and “asthma”; “CCL5”, “polymorphisms” and “asthma”) Additional studies were identified by hands-on searches ... association between RANTES -28C/G polymorphism and asthma risk Human studies, regardless of sample size, were included if they met the following criteria: (1) RANTES polymorphism at -28C/G were determined...

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Báo cáo y học: "Genetic polymorphisms in the nucleotide excision repair pathway and lung cancer risk: A meta-analysis"

Ngày tải lên : 31/10/2012, 14:59

... descriptive purposes (the XPA 23 polymorphism caused by the G-to-A substitution is the XPA G23A poly- Int J Med Sci 2007, morphism) The polymorphism, termed the XPA G23A polymorphism (at position 23 ... XPA G23A polymorphism may, thus, be a 62 promising SNP for lung cancer It is thought that cigarette smoking modifies the association between DNA repair polymorphisms, as well as metabolic polymorphisms, ... G23A polymorphism and smoking have not been fully elucidated, further studies are needed to better understand the associations between the XPA G23A polymorphism and lung cancer risk 3-3 ERCC1 polymorphisms...

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Báo cáo y học: "Grb2-associated binder 1 polymorphism was associated with the risk of Helicobactor pylori infection and gastric atrophy"

Ngày tải lên : 31/10/2012, 15:37

... (QIAGEN Inc.,Valencia,CA) A single nucleotide polymorphism (SNP) at intron of Gab1, named as JST164345 in a database of Japan Single Nucleotide Polymorphisms (JSNP) at http://snp.ims.u-tokyo.ac.jp, ... subjects could not be genotyped for PTPN11 Gab1 polymorphism and the step of atrophy/metaplasia-gastric cancer We assessed whether the Gab1 gene polymorphism was associated with the development ... This epidemiologic finding that the Gab1 polymorphism was associated with H pylori seropositivity and gastric atrophy is plausible However, the Gab1 polymorphism did not influence the development...

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Báo cáo y học: "Association of the T+294C polymorphism in PPAR δ with low HDL cholesterol and coronary heart disease risk in women"

Ngày tải lên : 31/10/2012, 17:03

... min., 65°C min., 72°C for 43 cycles and final extension at 72° for 10 MS-PCR created two fragment of divergent length which were detected by silver staining on polyacrylamid gel Statistical Analysis ... the ‫ ²א‬test The data was analysed using SPSS 12.0 software The association between the +294T/C polymorphism and serum lipids, coronary heart disease, and body mass index (BMI) was calculated ... interest exists References Discussion and Conclusions To address the question whether the +T294C polymorphism in PPARδ is associated with the risk for coronary heart disease and/or plasma lipid...

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Kế thừa (inheritance) và đa hình (polymorphism)

Ngày tải lên : 30/09/2013, 05:20

... tích III.6 Phương thức ảo (virtual method) tính đa hình (polymorphism) Hai đặc điểm mạnh kế thừa khả sử dụng lại mã chương trình đa hình (polymorphism) Đa hình ý tưởng “sử dụng giao diện chung ... thuc Encrypt cua giao dien IEncryptable public void Encrypt() { string KQ =""; for (int i = ; i< S .Length; i++) KQ = KQ + (char)((int)S[i] + 5); S = KQ; } // thuc thi phuong thuc Encrypt cua giao ... hướng đối tượng Phạm Quang Huy 2008 public void Decrypt() { string KQ =""; for (int i = ; i< S .Length; i++) KQ= KQ + (char)((int)S[i] - 5); S = KQ; } } // Thu nghiem chuong chinh public class...

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Advanced Types, Polymorphism, and Accessors

Ngày tải lên : 05/10/2013, 05:20

... code entry point 146 Chapter 7: Advanced Types, Polymorphism, and Accessors ■ Clearly, polymorphism comes with a computational overhead Adding polymorphism via virtual methods involves weighing ... signature of a method with that of the delegate 7.4 Polymorphism and Virtual Methods Unlike inheritance, which is a compile-time mechanism, polymorphism is the runtime ability of a reference to ... those sections of code that can afford the luxury of polymorphism In any case, it is always worthwhile to investigate the possibility since polymorphism leads to software that is more ﬂexible,...

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Exercise4. Inheritance, Polymorphism, and Virtual Functions Function Templates and the Standard Template Library

Ngày tải lên : 18/10/2013, 17:15

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Class dùng cho Fixed Length Text String

Ngày tải lên : 22/10/2013, 11:15

... in String ' Update Offset value for the next data field Offset = Offset + SubString.Size myLength = myLength + SubString.Size ' Add an SubString Object to the DataFields collection DataFields.Add ... SubString = Nothing Loop ' Fill the internal String with Blank spaces mvarTextStringData = Space(myLength) Close FileNum ' Close input file End Property Khi bạn chạy program mẫu kèm theo, trước hết ... field chứa characters Ngoài ra, ta dùng clsTextRecord để viết data trực tiếp vào Data field Fixed Length Text String Lưu ý cách dùng tên Data Fields listing Sub CmdGetSubStrings_Click() sau: Private...

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