restricting cursor movement to input cells
Tài liệu Báo cáo khoa học: Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells pdf
Ngày tải lên :
20/02/2014, 11:20
... step to remove nonadherent bacteria, trypsin (2.5 mgÆmL)1 in NaCl/Pi) was used to detach the cells from the surface and Triton X-100 (0.025% in NaCl/Pi) was then added to the cell suspension to ... adhesion of AP1 to epithelial cells and fibroblasts (Fig 5E) As S pyogenes adhesion has been shown to involve binding of fibronectin [20,40–42], we analyzed streptococcal binding to cells, depleted ... remaining bound to the streptococcal surface could mediate binding to proteins involved in host defence Known relevant ligands for GAGs include growth factors, cytokines and other mediators of inflammation...
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Báo cáo khoa học: Factor XII binding to endothelial cells depends on caveolae ppt
Ngày tải lên :
16/03/2014, 18:20
... binds to the cells in a manner identical to FXII [9], binding of FXIIa was used to quantify relatively the amount of FXII bound to cholesterol-depleted cells Normal and cholesterol-depleted cells ... caveolae is to investigate the detergent insolubility at °C To examine whether the FXII/receptor complex might be insoluble in cold Triton X-100, FXII was crosslinked to the receptor by the cleavable ... factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor Eur J Biochem 270, 111–118 13 Spisni, E., Griffoni, C., Santi, S., Riccio, M., Marulli, R., Bartolini, G., Toni,...
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Báo cáo Y học: Incorporation of 3-nitrotyrosine into the C-terminus of a-tubulin is reversible and not detrimental to dividing cells potx
Ngày tải lên :
17/03/2014, 10:20
... and the cytoskeleton fraction (which remained bound to the dish) was washed twice with mL prewarmed microtubule-stabilizing buffer Isolated cytoskeletons were immediately subjected to immunoblotting ... microtubules in cells cultured in the presence of 3-nitrotyrosine Tubulin carboxypeptidase is known to be associated with microtubules in living cells [24] Isolated cytoskeletons, freed of cytosolic ... possible link to cellular injury [11] were the first attempt to describe at the molecular level the role of free 3-nitrotyrosine as a cytotoxic agent In this case, morphological alterations of cells as...
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Báo cáo khoa học: Binding of activated Factor XII to endothelial cells affects its inactivation by the C1-esterase inhibitor pptx
Ngày tải lên :
23/03/2014, 20:22
... but also the HK/PPK complex to HUVEC Cells were incubated with the plasma for h, during which FXII was allowed to bind to the cells and to become activated Prior to the incubation, the plasma ... pulled off the cells by the binding of soluble FXIIa to the inactivator as illustrated in Fig 3A; (b) the second mechanism assumes that the inactivator binds to FXIIa, while still bound to the cell ... that it was due to either FXIIa or kallikrein adhering to the cells during cultivation It was subsequently shown that FXIIa generated by activation of FXII bound to cells responded to increasing...
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Báo cáo khoa học: Structure and function of human a-lactalbumin made lethal to tumor cells (HAMLET)-type complexes pptx
Ngày tải lên :
29/03/2014, 21:20
... of cell death in response to HAMLET Cytotoxicity of ELOA complexes Similar to HAMLET, the assembly of equine lysozyme and oleic acid into ELOA complexes led to cytotoxic activity ELOA effectively ... independently – is translocated to the nuclei in living tumor cells Apoptosis and macroautophagy in response to HAMLET HAMLET-treated cells show characteristics of apoptosis with typical changes in ... (red stain) internalization by tumor cells from 30 to h HAMLET is initially bound to the membrane of the cells and subsequently transported into the cells The cells maintain cellular integrity for...
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gene delivery to mammalian cells, volume 1
Ngày tải lên :
10/04/2014, 22:42
... pCMS-EGFP Vector pGFP Variant Vectors pIRES2-EGFP Vector EGFP Cotransfection Marker Vector pd2EGFP Vector Living ColorsΤΜ Fluorescent Timer Living ColorsΤΜ DsRed2 Vectors pDsRed Vector Promoterless ... Vectors N-terminal Enhanced Fluorescent Protein Vectors C-terminal Enhanced Fluorescent Protein Vectors Promoterless Destabilized Vectors SEAP SEAP b-galactosidase b-galactosidase b-galactosidase ... Incubate cells in a CO2 incubator for 30–90 Tap cells occasionally to keep them from clumping Incubation times vary and should be determined experimentally Add DMSO to cells dropwise to a final...
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gene delivery to mammalian cells, volume 2
Ngày tải lên :
11/04/2014, 00:48
... dose-dependent vector toxicity limited vector persistence (13,46) Therefore, a dose high enough to achieve efficient transgene expression and low enough to avoid hepatotoxicity is required To complicate ... effector cells, such as neutrophils, into the liver This acute-phase toxicity does not require expression of viral genes but is dependent on the dose of vector: minimal toxicity has been shown to ... vectors From: Methods in Molecular Biology, vol 246: Gene Delivery to Mammalian Cells: Vol 2: Viral Gene Transfer Techniques Edited by: W C Heiser © Humana Press Inc., Totowa, NJ Douglas to be...
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báo cáo hóa học:"Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells" pdf
Ngày tải lên :
18/06/2014, 15:20
... unresponsive to CD66ae and CD66de mAbs, but they remained responsive to CD66b, CD66c, and CD63 mAbs (Fig 1, panel D) When cells were desensitized to CD66b and CD66c mAbs, the cells were unresponsive to ... have been shown to be capable of activating neutrophils as determined by the physiologic response of adhesion to human umbilical vein endothelial cells (HUVECs) [37] CD66 mAb binding to the neutrophil ... desensitization to pairs of CD66a, CD66b, CD66c, and CD66d mAbs Desensitization of neutrophils to further stimulation by mAbs directed to specific CEACAM family members by exposure of the neutrophils to the...
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Báo cáo y học: "CD134 as target for specific drug delivery to T cells in adjuvant arthritis" pps
Ngày tải lên :
09/08/2014, 06:22
... centrifugation and transferred to round-bottomed 96-well plates at 105 cells per sample For studying anti-CD134 liposome binding to T cells in vitro by flow cytometry, A2b cells were incubated with ... cells to Mt HSP60211–225, which was reported not to be related to AA [16], indeed indicated that a part of the CD134+ T cells was not activated in relation to clinical disease but responded to ... M, Itoh M, Toda M: Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25) Breakdown of a single mechanism of self-tolerance causes various autoimmune...
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Báo cáo y học: "Prior mucosal exposure to heterologous cells alters the pathogenesis of cell-associated mucosal feline immunodeficiency virus challenge" docx
Ngày tải lên :
13/08/2014, 01:20
... been to examine the effect of mucosal exposure to the challenge cells (Mya-1) It is reasonable to speculate that prior exposure to uninfected Mya-1 cells would induce the strongest protection to ... exposed to heterologous cells and allogeneic material during sexual activity Ejaculates contain HLA antigen expressing CD4+ T cells, macrophages, neutrophils, germ cells, epithelial cells and to some ... Taken together, there is strong evidence that exposure to heterologous cells and allogenic material alters the susceptibility of cells to lentiviral infection However, whether this translates into...
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Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
Ngày tải lên :
13/08/2014, 05:21
... replication and transfer of HIV-1 to permissive cells TERT-2 cells appeared to transfer both HIV-1 strains to PBMCs (Fig 1B), with average effectiveness when compared to the TE cells from different donors ... within the TERT-2 cells to infect co-cultured MOLT4/CCR5 cells When TERT-2 cells were treated with colchicine (500 μM for 30 before inoculation) to uncouple the tubulin cytoskeleton, 18% of the ... in triplicate to permissive cells in vitro HIV trans infection in vitro from primary TE cells to PBMCs showed variation among tonsil donors Since TE cells were derived from excised tonsils obtained...
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Báo cáo y học: " Porphyromonas gingivalis induces CCR5-dependent transfer of infectious HIV-1 from oral keratinocytes to permissive cells" doc
Ngày tải lên :
13/08/2014, 06:20
... presented to reporter TZM-bl cells to assay for infectious HIV-1 From to 54 h post-inoculation, TERT-2 cells pre-incubated with P gingivalis released significantly more infectious R5-tropic HIV1 into ... from TERT-2 cells (Fig 4B) After removing the plasma membrane-associated virions, cells were lysed to recover internalized HIV1 Lysates were inoculated onto the reporter TZM-bl cells to assess ... shown) to TZM-bl cells After pre-incubation with P gingivalis, anti-CCR5 reduced trans infection of R5-tropic HIV-1 to levels similar to HIV-1 inoculated TERT-2 cells without P gingivalis To confirm...
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Báo cáo y học: " Dendritic cell-mediated HIV-1 transmission to T cells of LAD-1 patients is impaired due to the defect in LFA-1" docx
Ngày tải lên :
13/08/2014, 09:20
... added an inhibitor of the viral protease (saquinavir, [23,24]) To distinguish virus transmitted to T cells from HIV in DC, we co-stained with CD3 and DC-SIGN DC and T cells tend to cluster, which ... both in C33A cells and in PM1 T cells C33A cells not express ICAM-1 (or LFA-1), yielding virions without ICAM-1 With both virus stocks, we found impaired DC-mediated transmission to T cells of LAD-1/variant ... EDTA to our FACS buffer to reduce the amount of cell clustering Indeed, the majority (95%) of the CD3 positive cells was negative for DC-SIGN, showing that only a few DC were attached to T cells...
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Báo cáo y học: "TGF-β dependent regulation of oxygen radicals during transdifferentiation of activated hepatic stellate cells to myofibroblastoid cells" potx
Ngày tải lên :
13/08/2014, 13:20
... well as plateletderived growth factor (PDGF) receptors α and β are shown to be regulated which points to their regulatory functions in establishing resistance to oxidative stress Results and discussion ... reduces radical accumulation in myofibroblastoid cells These defense mechanisms are suggested to be particularly relevant in order to protect myofibroblastoid cells from harmful consequences caused ... phagocytic cells such as neutrophils and macrophages In addition, several non-phagocytotic cell types such as hepatocytes [2] and hepatic stellate cells (HSCs) [3] have also been shown to express...
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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"
Ngày tải lên :
25/10/2012, 11:18
... Autologous hematopoietic stem cell transplantation in autoimmune diseases Expert Rev Hematol,2009 Dec;2(6):699-715 12 Rosato E, Pisarri S, Salsano F Current strategies for the treatment of autoimmune ... were ischemic, stem cells in circulation are adherent to the vascular endothelial cells, cross the endothelial cells, migrate and finally reached at ischemic sites Inflammatory may be observed ... of stem cells, it is very important to observe the survival and curative effects of transplanted stem cells Traditional immunohistochemical method can easily identify the transplanted cells with...
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Sensibility study of flooding and drying issues to the operating conditions in PEM fuel cells
Ngày tải lên :
05/09/2013, 14:58
... 1, 2010, pp.1-20 16 Qtot = Ptot vtot Ach R Top (25) So, the cathode velocity is: vtot = Qtot R Top Ptot Ach (26) A high total pressure therefore produces a low velocity (the total molar flow is ... mixture in the channel vtot (m.s-1) is computed in each slice using the relationship (20) P (20) Qtot = tot vtot Ach R Top where Ach is the channel area (m2) and Ptot the total pressure in the ... A.cm-2), it is sufficient to hold the oxygen stoichiometric ratio at a minimum of For high currents (around A.cm-2), the stoichiometric ratio has to be increased up to 10 in order to avoid water condensation...
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Optimal design of PEM fuel cells to generate maximum power: A CFD study
Ngày tải lên :
05/09/2013, 14:58
... resistance of the proton transport across the membrane The potential loss in the membrane is due to resistance to proton transport across the membrane from anode catalyst layer to cathode catalyst ... performance to investigate the in situ total displacement and degree of the deformation of the polymer membrane of PEM fuel cells The model is shown to be able to understand the many interacting, ... pressure due to the enhanced reaction kinetics To reduce mass transport loss, the cathode is usually run at high pressure In essence, higher pressures help to force the oxygen and hydrogen into contact...
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Piping Input to read
Ngày tải lên :
05/10/2013, 08:51
... 70 CHAPTER 10 ■ PIPING INPUT TO READ Pipe to read Directly The next example represents a scenario in which the script does not pipe to a loop, but instead pipes input to a read statement This ... $foo | cut -c1-` Process Input Word-by-Word The last example enables you to parse through each word of some input string, consuming two words at a time Words are assumed to be separated by spaces ... 10 ■ PIPING INPUT TO READ Line-by-Line Option This is the here-document workaround option where we remove the need for a temporary file This functions in the same way as Option the _input= `ps -ef`...
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Tài liệu Báo cáo khoa học: Affinity and kinetics of proprotein convertase subtilisin ⁄ kexin type 9 binding to low-density lipoprotein receptors on HepG2 cells docx
Ngày tải lên :
14/02/2014, 14:20
... chase medium was adjusted to 6.2 with Mes at °C Exposure of cells to low pH did not damage the cells during the chase period ( h), as judged by the ability of cells to exclude Trypan blue dye ... [125I]TC-PCSK9 to HepG2 cells grown in the absence of lipoproteins It is more likely that the observed heterogeneity in binding of [125I]TC-PCSK9 to HepG2 cells is attributable to binding to different populations ... We initially attempted to investigate the binding of PCSK9 to HepG2 cells using PCSK9 directly labeled with 125I 2946 HepG2 cells (European Collection of Cell Cultures, Porton Down, UK) were routinely...
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