responses of lactic acid bacteria to acid stress

... immune responses upon a variety of stimuli, including lactic acid bacteria (LAB) LAB are a group of related bacteria that produce lactic acid as a result of carbohydrate fermentation Lactobacilli, ... Tumor growth factor Th - T helper cell TLRs - Toll like receptors TNF - Tumor necrosis factor Tollip - Toll inhibitory protein TRAF6 - TNF receptor associated factor TSA - Tryptone Soy Agar U/ml ... THE ROLES OF LACTIC ACID BACTERIA IN HOST INFLAMMATORY RESPONSES WANG SHUGUI (B.Sc., Sun Yat-Sen University) A THESIS SUBMITTED FOR THE DEGREE OF PHILOSOPHY OF DOCTORATE DEPARTMENT OF MICROBIOLOGY...

Ngày tải lên: 13/09/2015, 19:50

... the total amount of lactic acid in the medium and the buffering capacity of the medium are known The amount of undissociated lactic acid is then further related to the total amount of lactic acid ... filtration of the sample to remove the cells (Sarstedt, pore size 0.2 mm) and derivatisation of the lactic acid present in the filtrate with methanol, lactic acid was measured through gas chromatographic ... theoretical analysis of the model Some mathematical properties of the model are discussed, and an easy -to- use method to take into account the evolution of pH and undissociated lactic acid [LaH] in the...

Ngày tải lên: 05/05/2014, 08:44

... general, the acid tolerance of LAB depends on the pH profile of H?-ATPase and on the composition of the cytoplasmic membrane This is largely influenced by the type of bacterium, the type of growth ... bile acids are less inhibitory than free bile acids (cholic and deoxycholic acid, DCA) toward intestinal aerobic and anaerobic bacteria Taurine-conjugated deoxycholic acid (TDCA) was less toxic ... are evaluated as beneficial bacteria by their product of acids (lactic acid) , bacteriocin or bacteriocin-like substances Putrefactive bacteria are regarded as harmful bacteria in that they decompose...

Ngày tải lên: 07/07/2014, 19:20

... 11.0 30 1.3.3.1 Lactic acid bacteria Lactic acid bacteria (LAB) are a group of related bacteria that produce lactic acid as major product.31 LAB have the property of producing lactic acid from carbohydrates ... CECT-4023T to ferment 17.4 g/L of xylose and 11.1 g/L of glucose using hemicellulose hydrolysate of vine shoots to give 21.8 g/L of lactic acid A list of lactic acid bacteria that are able to ferment ... 1.3.3 Lactic acid microorganisms Bacteria and fungi are the two groups of microorganisms that can produce lactic acid. 17 While most of the lactic acid production are carried out by lactic acid bacteria...

Ngày tải lên: 16/10/2015, 11:57

... rate and stomata conductance of Sa and Sc were relatively stable, which showed the adaptation of the two species to low salinity Net photosynthesis rate and stomata conductance of Rs rose to the ... salt tol- nonstomatal components of photosynthesis and dissipation of erance and induces biosynthesis of both mannitol and a gluco- excess light energy Planta, 1992, 187: 335–347 syl-mannitol ... photosynthesis rate of Sa, Sc and Rs Results and analysis 2.1 Effects of salinity on leaf net photosynthesis rate, stomata conductance and transpiration rate of Sa, Sc and Rs The indices of leaf...

Ngày tải lên: 05/07/2014, 23:21

... ontogeny and resource availability We expected the reduction of seedling [St] with fertilization to be a consequence of higher carbon consumption by growth (hyptothesis 1), but the lack of DM responses ... as water stress limits both carbon and nitrogen gain [42] The aim of the present study was to assess the combined response of growth, carbohydrate and nitrogen reserves of seedlings of three ... species followed a clear Stpool -[St] trajectory in response to an increase of water Oak seedlings responses to nutrient and water 207 Figure Effects of fertilization (left) and watering (right)...

Ngày tải lên: 07/08/2014, 16:20

... of total leaf area to total leaf biomass Leaf area index was estimated from total leaf area per plant and number of plants per clone and per ground area of the ) ring (50 m Stem diameters of ... day The height of the plenum may be increased by means of extensive legs This has permitted us to follow the growth of plants and allowed for CO fumigation of the plant canopy up to m in height ... for growth and survival of plants Osmotic adjustment in leaves of plants exposed to elevated [CO due to enhanced con] centrations of soluble sugars [3] might allow them to maintain higher relative...

Ngày tải lên: 08/08/2014, 14:21

... primary factor for variations in SUV after treatment was the reduction of metabolism due to cellular death or less likely, in case of an effective treatment, augmentation of metabolism due to tumor ... changes of size versus SUV with the axes cross the metabolic response line of -25% and anatomical response The percentage changes of size versus SUV with the axes cross the metabolic response line of ... Page of (page number not for citation purposes) Radiation Oncology 2007, 2:18 tion of the metabolism to the required response level (left upper quadrant of Fig 1) This patient was found later to...

Ngày tải lên: 09/08/2014, 10:21

... act to stabilise associations of CD47/ IAP with integrins, whereas the cytoplasmic tail may be involved in regulation of the cytoskeleton through PLICs (proteins linking IAP to cytoskeleton) ... leads to activation of tetrodotoxin-sensitive channels, sodium influx, and cell membrane depolarisation To establish whether CD47/IAP has roles in the response of articular chondrocytes to 0.33-Hz ... adhesion to fibronectincoated surfaces without affecting cell adhesion to those surfaces and to inhibit activation of neutrophil polymorphonucleocyte activity, including phagocytosis, respiratory...

Ngày tải lên: 09/08/2014, 10:22

... different2 of the number of ESTs responsive to UV-B supplementation in Summarytissues of b, pl maize plants Summary of the number of ESTs responsive to UV-B supplementation in different tissues of b, ... leaves under experimental protocol RNA gel-blot analysis to study the kinetics of UV-B induction of gene RNA gel-blot analysis to study the kinetics of UV-B induction of gene expression in adult ... gel-blot analysis to study the kinetics of UV-B induction of gene RNA gel-blot analysis to study the kinetics of UV-B induction of gene expression in 14-day-old roots Lanes contained 10 µg total RNA...

Ngày tải lên: 09/08/2014, 20:20

... and direct injection routes of administration of × 108 bacteria and p = 0.003 for comparison of the two routes of administration of × 108 bacteria) Finally, each of pregnant dams who received ... killed bacteria, a small chip of the stock culture was thawed in Luria-Bertani (LB) medium, passaged times prior to overnight culture in L of medium and concentrated by centrifugation The bacteria ... ml of PBS, yielding a concentration of 1.7 × 1011 bacteria per ml This dense live bacterial prep was quantitated by plating serial dilutions for overnight culture just prior to the remaining stock...

Ngày tải lên: 11/08/2014, 07:21

... Score: to 3) were obtained post mortem (within 24 hours after death) from the knee joints of a total of six donors with no clinical history of joint disorders (mean age: 56 years, range: 43 to 65 ... serum (complete medium) and ng/ml of Transforming Growth Factor b1 (TGFb-1), ng/ml of Fibroblast Growth Factor 2, and 10 ng/mL of Platelet-Derived Growth Factor-BB (all from R&D Systems, Minneapolis, ... placed in a vibratory microtome (VT 1000 E, Leica, Heidelberg, Germany) to trim off the outermost, approximately 150 μm thick cartilage layer parallel to the support surface to minimize inhomogenities...

Ngày tải lên: 12/08/2014, 11:23

... bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic use Acida ... Lactobacillus HJL-4 Lactobacillus HJL-20 Lactobacillus HJL-24 Lactobacillus HJL-25 Lactobacillus HJL-30 Lactobacillus HJL-32 Lactobacillus HJL-33 Lactobacillus HJL-34 Lactobacillus HJL-37 Lactobacillus ... benefits from lactic acid bacteria FEMS Microbiol Rev 1990, 7, 175-188 11 Gilliland SE, Walker DK Factors to consider when selecting a culture of Lactobacillus acidophilus as a dietary adjunct to produce...

Ngày tải lên: 07/08/2014, 18:20

... of inflammatory cells following a direct topical contact [11] In addition, some Lactic Acid Bacteria (LAB) have shown immunomodulatory and anti-inflammatory activities The genus Lactobacillus ... amplification of inflammatory reaction [46] The reduction of this pro-inflammatory cytokine following MPM consumption indicated its potential in the inhibition of development of inflammatory disease ... measured every day Page of 10 (page number not for citation purposes) Journal of Inflammation 2007, 4:6 Prophylactic protocol – Mouse ACD The prophylactic anti-inflammatory potential of MPM was evaluated...

Ngày tải lên: 11/08/2014, 08:21

... value of fruit juice fermented by lactic acid bacteria (LAB) Four isolates of lactic acid bacteria from mango were obtained The results of research showed that all 10 strains of lactic acid bacteria ... isolates were able to survive and grow at this pH The cause of the decrease in pH was due to the development of lactic acid bacteria that produced lactic acid Content of lactic acid produced from ... products stored at - 60C and content of lactic acid bacterial density almost stable after weeks of storage Content of lactic acid from 1.07 to 1.1 %w/v and bacterial density 8.51 20 8.16 (log...

Ngày tải lên: 06/10/2015, 12:57

... Isolation of lactic acid bacteria - Study on low pH tolerance of isolates - Study on lactic acid fermentation ability of isolates in carrot juice - Identification of the selected lactic acid bacteria ... To isolate and select lactic acid bacteria and to study the favorable conditions of lactic acid fermented carrot juice with biological values The content of this study includes: - Isolation of ... Isolation of lactic acid bacteria 3.2 Study on low pH tolerance of isolates 3.3 Study on lactic acid fermentation ability of isolates in carrot juice 10 3.4 Identification of the...

Ngày tải lên: 06/10/2015, 13:04

... organic acids,… Dragon fruit juice is a suitable medium for lactic acid bacteria to produce new probiotic products Therefore, the study “Fermentation of dragon fruit juice by lactic acid bacteria ... isolated bacteria strains belonged to lactic acid bacteria because they: - Are Gram-positive bacteria - Has no enzyme catalase - Are rod-shaped or streptococcus - Can grow on MRS medium 3.2 Acidity tolerant ... concentrations of bacteria began to reduce due to the inhibition of acid against bacteria (less than 6.0 log cells/mL) Thus, these temperatures were not suitable for storing products The samples stored...

Ngày tải lên: 06/10/2015, 13:04

... for lactic acid bacteria to produce new probiotic products Therefore, the research “Fermentation of pineapple juice by lactic acid bacteria was carried out Objectives To isolate lactic acid ... hours because of converting sucrose to lactic acid by LAB L acidophillus from Ybio probiotic powder had a preponderance of lactic acid content LAB density at initial inoculum was close to inoculum ... sugar into lactic acid inhibited the bacterial growth and development Besides, lactic acid itself could inhibit the development of bacteria (Nguyen Thi Hien, 2006) In contrast, the treatment of 15...

Ngày tải lên: 06/10/2015, 13:05

... Poly (lactic acid) fabric Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting ... [PLA] Figure Mean [Ā] of effect of time of heatsetting on the tensile extension EM [%] of Ingeo™ Poly (lactic acid) 3.2 Linearity of load extension curve LT [-] The linearity of load extension curve ... Ingeo Poly (lactic acid) (PLA) fabric Conclusion The effect of time of heatsetting on tensile properties of knitted Ingeo™ Poly (lactic acid) (PLA) was determined using the KES-FB system of fabric...

Ngày tải lên: 05/09/2013, 16:10

... Gregori et al membrane In the cytoplasm, weak acids encounter a more neutral pH, causing their dissociation into acid anions and protons The protons lead to cytoplasmic acidification, thereby inhibiting ... essential component of this stress response pathway, as cells are hypersensitive to sorbic, benzoic and propionic acid [7] and fail to adapt to such stress conditions in the absence of Pdr12p Moreover, ... in the presence or absence of the stress agent [15] Thus, the lack of PDR12 stress induction by War1-42p is perhaps due to its inability to bind to the promoter WARE of its target gene Alternatively,...

Ngày tải lên: 19/02/2014, 00:20