resistance sensitivity to glucocorticoid induced apoptosis

... with the reduced sensitivity to TNF-a stimulation (Fig 3D,E) Thus, the partial cross -resistance to TNF-a -induced apoptosis is not caused by a general suppression of death receptor transcription ... KH, Oshiro MM, Buyuksal I, Painter JS & Dalton WS (1999) Myeloma cells selected for resistance to CD95-mediated apoptosis are not cross-resistant to cytotoxic drugs: evidence for independent mechanisms ... the reduced sensitivity to TNF-a was associated with acquired Fas resistance U937 cells have been reported to express both tumour necrosis factor receptor (TNFR1) and TNFR2 [19] To investigate...

Ngày tải lên: 23/03/2014, 06:20

... cell's sensitivity to chemotherapy Indeed, cancer cells develop resistance to chemotherapy by means of inactivating apoptotic factors and enhancing survival pathways that antagonize apoptosis ... K-Y, Broxmeyer HE: Blocking of c-FLIPLindependent cycloheximide -induced apoptosis or Fas-mediated apoptosis by the CC chemokine receptor 9/TECK interaction Blood 2001, 98:925-933 12 Brunet A, Bonni ... CCL25 -induced cisplatin resistance of OvCa cell lines Treatment of OVCAR-3 and SKOV-3 cell lines with cisplatin alone resulted in 96% and 95% respective increases in apoptosis relative to the...

Ngày tải lên: 20/06/2014, 07:20

... that the resistance to anti-Fas -induced apoptosis in lung fibroblasts is mediated by the overexpression of the specific inhibitors of apoptosis X-chromosome-linked inhibitor of apoptosis (ILP) ... short-lived inhibitory proteins and led to increased susceptibility to Fas-mediated apoptosis We used cycloheximide to analyse whether the differences in sensitivity to FasL -induced apoptosis depended ... significantly more apoptosis resistance D: Increased resistance to pro-apoptotic signals in fibrotic fibroblasts (square) compared to control fibroblasts (circle) Apoptosis was induced by rhFasL...

Ngày tải lên: 12/08/2014, 18:21

... carcinoma cells, but not human hepatocytes, to TRAIL Hepatology 42, 588–597 38 Kurland JF & Meyn RE (2001) Protease inhibitors restore radiation -induced apoptosis to Bcl-2-expressing lymphoma cells ... induces apoptosis by binding to its agonistic cognate cell surface receptors death receptor (DR4) ⁄ TRAIL R1 and death receptor (DR5) ⁄ TRAIL R2, and this then leads to recruitment of the adaptor ... a clear trend towards an apoptosis- inhibitory effect of the DR5 knockdown, indicating that basal expression of DR5 is required for the TRAIL sensitivity in these cells Apoptosis induced by TRAIL...

Ngày tải lên: 18/02/2014, 17:20

... methadone to its variants [9], it is presently not possible to determine whether and to what extent this could influence sensitivity to respiratory depression In summary, studies are needed to elucidate ... genetically mediated sensitivity to methadone -induced respiratory depression Another factor that may be involved in methadone pharmacokinetics is the strong blood binding of methadone to plasma proteins, ... polymorphism in the µ-opioid receptor appears an interesting candidate for contributing to interindividual variability in sensitivity to opioid agonist induced respiratory depression, caution should...

Ngày tải lên: 13/08/2014, 03:20

... resistant to H2O2 -induced apoptosis Fig-31: Sensitivity of HCT116 cells to H2O2 -induced apoptosis is Bax dependent Fig-32: Downstream cytochrome C release is absent in Bax knockout cells in response to ... RSV inhibit H2O2 -induced apoptosis in HL-60 cells 4.3.2 RSV mediated inhibition of H2O2 induced apoptosis is upstream of the mitochondria 4.3.3 RSV inhibits H2O2 -induced apoptosis by creating ... tilt” in favor of apoptosis To substantiate the “redox hypothesis” on the fact that cells can be primed by increase production of O2- to become resistant to drug induced apoptosis I used another...

Ngày tải lên: 16/09/2015, 17:11

... mitochondrial-derived hydrogen peroxide is an effector mechanism for drug -induced apoptosis in tumor cells J Biol Chem 2001;276:514 –21 Clement MV, Ponton A, Pervaiz S Apoptosis induced by hydrogen peroxide is ... for the sensitivity of leukaemic cells to etoposide -induced apoptosis Oncogene 2001;20:4817–26 27 Putcha GV, Deshmukh M, Johnson EM Jr BAX translocation is a critical event in neuronal apoptosis: ... some cell types to H2O2, the inhibitory effect of Bcl-2 overexpression (12) supports the involvement of the mitochondrial death pathway in H2O2 -induced apoptosis However, as the mitochondria are...

Ngày tải lên: 16/09/2015, 17:11

... in the presence of RSV to vincristine -induced apoptosis (Fig 6, C and D) The inhibitory effect of low concentrations of RSV on druginduced apoptosis was not exclusive to C2 or vincristine Preincubation ... Similar to DPI, transient transfection with RacN17 completely neutralized the death-inhibitory activity of RSV and restored the sensitivity of leukemia cells to H2O2 (Fig 3E) RSV Inhibits Apoptosis ... response to C2 (Fig 5D) To gain additional insight into the death-inhibitory effect of RSV and its potential clinical implications, we next investigated the effect of RSV on apoptosis induced...

Ngày tải lên: 16/09/2015, 17:11

... expression increases sensitivity to H2O2-mediated apoptosis in complete contrast to the protective effect of other apoptosisinducing agents Increased sensitivity is primarily due to the transcriptional ... Rat1 cells to H2O2 treatment and had no effect upon apoptosis induced by treatment with taxol (Fig S2) caIII expression was restored in 5.61 cells to determine if the sensitivity to H2O2 treatment ... oxidant -induced apoptosis 5.62 cells that is absent from Neo1, Neo2 and parental Rat1 cells (Fig 1B) Previous studies have shown that Evi1 is a survival factor, protecting cells from apoptosis induced...

Ngày tải lên: 29/03/2014, 08:20

... initial DNA damage and the radiationinduced apoptosis, as a consequence of x-ray, may exist [43,44] DNA DSB are assumed to be the most important lesion to induce apoptosis [45] Depending on the severity ... cells may undergo apoptosis instead of attempting to repair the damage [46] Lymphocytes are particularly sensitive to apoptosis, partly because they induce Bax expression in response to ionizing radiation ... Defective apoptotic response to radiation in PBLs could help to explain this inverse relation [14] According to the above observations, high initial DNA damage [17] or low radiation -induced apoptosis...

Ngày tải lên: 09/08/2014, 09:20

... chondrocyte apoptosis J Cell Physiol 197, 379–387 An WG, Hwang SG, Trepel JB & Blagosklonny MV (2000) Protease inhibitor -induced apoptosis: accumulation of wt p53, p21WAF1 ⁄ CIP1, and induction of apoptosis ... TRAIL -induced apoptosis under normoxic and hypoxic conditions We found that TRAIL and ALLN alone did not induce apoptosis, but combined treatment of the cells with ALLN and TRAIL increased apoptosis ... considered to be a key factor in the growth and survival of chondrocytes Hypoxia is known to regulate the expression of many genes, but little is known about its role in either apoptosis or anti -apoptosis, ...

Ngày tải lên: 18/02/2014, 14:20

... expecting to reveal new molecules related to PYM resistance, and to provide candidate biomarkers to predict the clinical response to PYM-based chemotherapy in tongue cancers Results Biological characteristics ... suppress the antiapoptotic activity of Tca8113 ⁄ PYM cells to enhance the PYM effect To further observe the enhanced effect of CA9 interference on PYM -induced Tca8113 ⁄ PYM apoptosis, caspase activity ... PYM -induced drug resistance in tongue cancer Using cDNA microarray technology, we have identified 89 genes and 13 ESTs that may be related to PYM-inducible resistance In particular, CA9 seems to...

Ngày tải lên: 06/03/2014, 22:21

... inhibition of apoptosis Hypoxia has been reported to inhibit apoptosis induced by tumor necrosis factor-related apoptosisinducing ligand (TRAIL) [14,15], which selectively induces apoptosis in malignant, ... only TRAIL induces apoptosis The inability of hypoxia to cause apoptosis was not surprising, considering the number of reports demonstrating that hypoxia blocks TRAIL -induced apoptosis There are ... enhancement of apoptosis over that induced by TRAIL in the presence of the empty vector Thus, the data indicate that expression of full-length BRCA1 augments TRAIL -induced apoptosis We also investigated...

Ngày tải lên: 30/03/2014, 03:20

... ligand (TRAIL) -induced apoptosis tosis-inducingof proliferating rheumatoid arthritis synovial fibroblasts (RASFs) to Fas ligand (FasL) -induced and tumour necrosis factor-related apoptosis- inducing ... (CD95) is a well-known apoptosis- signalling cell surface receptor belonging to the TNF receptor family [36] To investigate the susceptibility of RASFs to FasL-mediated apoptosis, cell surface expression ... (8 d) were more sensitive to TRAIL -induced (e) and FasL -induced (f) apoptosis than proliferating RASFs in S phase (9/1 d) or G2/M phase (9/2 d) Staurosporin -induced apoptosis was measured as a...

Ngày tải lên: 09/08/2014, 01:22

... Because the BN rat displays a dichotomy of resistance to infection-triggered arthritis but susceptibility to mercury -induced immune disruption, we addressed how these factors would interact if they ... investigators to address response to HgCl2 [5,6,8,9] Roos and colleagues [13,14] used a two-injection abbreviated schedule to investigate the short-term effects of HgCl2 exposure This protocol was ... resistance to ReA can be overcome by heavy metal exposure Mercury exposure alters the cytokine profile, host clearance capability, and histopathological outcome from the resistant phenotype to...

Ngày tải lên: 09/08/2014, 01:22

... [8,9] Apoptosis is a physiological process and is a highly selective way to eliminate aged and injured cells In addition to internal pathways that trigger apoptosis mainly in response to cytotoxic ... Biogerontology 2002, 3:85-88 Baier A, Meinecke I, Gay S, Pap T: Apoptosis in rheumatoid arthritis Curr Opin Rheumatol 2003, 15:274-279 Pope RM: Apoptosis as a therapeutic tool in rheumatoid arthritis ... inhibitory effects on OA tenocytes and prevented these cells from undergoing FasL induced apoptosis in a dose dependent manner (Fig 4b) In contrast, no inhibitory effects of TNF-α on FasL induced apoptosis...

Ngày tải lên: 09/08/2014, 01:23

... 3, 21 to 50 osteoclasts per paw; and 4, more than 50 osteoclasts per paw Immunohistochemistry Immunohistochemical staining on sequential sections (8 to 12 paws per group) was performed to detect ... performed to detect the presence of osteoclasts (Sigma-Aldrich) TRAP staining was scored semi-quantitatively (on a scale from to 4): 0, no osteoclasts per paw; 1, to 10 osteoclasts per paw; 2, 11 to ... inflammatory cells in the inflamed synovium with a detrimental effect on RA To test our hypothesis, we used a well-established mouse model for RA, murine collagen -induced arthritis (CIA) [16], to...

Ngày tải lên: 09/08/2014, 08:22

... profiles were not due to differential inflammatory leukocyte recruitment between groups Bleomycin -induced histopathology in wild-type mice as compared to cathepsin K transgenic mice Histopathological ... according to the manufacturer's instructions to visualize protease activity Immunohistochemistry Formalin-fixed lung specimen from sham-treated wildtype mice and cath K tg mice were subjected to immunohistochemical ... to a pneumotachograph (HSE) connected to a differential pressure transducer to determine tidal flow Trans-pulmonary pressure (PTP) was measured via a water-filled esophagus catheter coupled to...

Ngày tải lên: 12/08/2014, 15:21

... and stroke, inflammatory bowel disease, arthritis, toxic shock and acute reperfusion injuries [15-18] Thus peroxynitrite, and not NO, has been proposed to be the ultimate cytotoxic species in many ... The realization of the cytotoxic potential of NO and peroxynitrite made it important to seek for pharmacological approaches, in order to neutralize NO and peroxynitriteinduced damage by inhibiting ... transforming growth factor-beta J Cell Biol 1987, 105:1039-1045 Pierce GF, Mustoe TA, Lingelbach J, Masakowski VR, Gramates P, Deuel TF: Transforming growth factor reverses the glucocorticoid- induced wound-healing...

Ngày tải lên: 12/08/2014, 18:21

... report that aPC is able to prevent endothelial morphological injuries and to increase the sensitivity to the vasoconstrictor agent PE in a well-documented rabbit endotoxin -induced shock model [7-12] ... compared to the LPS group, suggesting the ability to respond to further endotoxin stimulation In vitro vascular reactivity Vascular contraction The maximal vasoconstrictor response to PE was ... receptor-dependent endothelium-dependent vasodilator agonist ACh (10-9 to 3.10-5 mmol/l); the receptor-independent endothelium-dependent vasodilator agonist calcium ionophore A23187 (10-9 to 3.10-6...

Ngày tải lên: 12/08/2014, 23:22