republican use of repressive doctrines against federalists and dissident republicans alike

... Courts: Federalists Against Republicans, Republicans Against Federalists, and Republicans Against Dissident Republicans in Struggles for Party Control 187 A Introduction 187 B Republican Use of Repressive ... Republican Use of Repressive Doctrines Against Federalists and Dissident Republicans Alike C 188 Pennsylvania 188 a Republicans Against Federalists 188 b Republicans Against Republicans 194 New York ... dozens of cases concentrated in Connecticut, Massachusetts, New York, and Pennsylvania, Federalists, when in power, used the doctrine against Republicans, and Republicans, when in power, used the...

Ngày tải lên: 07/12/2015, 00:59

... Objectives of the audit; • Summary of audit methodologies used for collecting and analysing data and indication of sources of data; • Explanation of criteria, such as benchmarks, used to interpret ... objectives? • Evaluation of the impact of the policy: What is the economic and social impact of a particular policy? • Evaluation of the effectiveness of the policy and a cause effect analysis: Are ... Scope and completeness in the planning and performance of audits carried out; • Reliability and validity of the opinions, or findings and conclusions, appropriateness of the recommendations and...

Ngày tải lên: 06/03/2014, 23:20

... relation pairs of a verb and its translation equivalent Let a structure of natural categories of nouns were given (independently of verbs) A part of the categories (concepts) structure and associated ... category, ,numbers of associated pairs of verb and its translation equivalent are generally small and can easily be found (3) Association of the pair of verb and its translation equivalent through ... (2) To each verb found in the process of the association, consults ordinary dictionary of translation equivalents and word usage of verbs and obtain the set of all the translation euqivalents for...

Ngày tải lên: 31/03/2014, 17:20

... extraction of bioflocculants from activated sludge, followed by steaming extraction SDS and washing extraction methods were ineffective because of low flocculating activity, and low yield and low ... the elution profiles The profile of steaming-extracted polymer (A) contained two saccharide peaks (at elution volumes of 70 ml and 90 ml) and one UV-absorbing peak (at elution volume of 95 ml) The ... reagent grade Results and Discussion Yields and chemical components - 148 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 Table Yields and Chemical Components of Polymers Extracted...

Ngày tải lên: 05/09/2013, 09:08

... research procedure, the scope of the study, the significance of the study and the design of the study Part 2: Development consists of Chapter one “Literature Review” and Chapter two “Action Research ... part which offered a summary and suggestions for more effective writing activities and some limitations and suggestions for further studies VII SIGNIFICANCE OF THE STUDY Writing, one of the two ... classroom” (Grebhard and Oprandy,1999) In the “Longman Dictionary of Language Teaching and Applied Linguistics” Richards, J.C and Platt, J.H gave the following definitions of action research:...

Ngày tải lên: 07/09/2013, 13:58

... activation of a family of cytosolic cysteine proteases, caspases, which are required for many of the morphological changes that occur during apoptosis The mitochondrial release of cytochrome c and second ... between EGF-induced reduction in Bid and tBid levels and the ubiquitin ligase activity of Itch In this study, we first examined the ability of Itch to interact with Bid and tBid We found that Itch specifically ... cells were cotransfected with constant amounts of rLuc–Itch and various amounts of either Bid–GFP of tBid–GFP The graph is a representative example of the saturation studies performed to provide...

Ngày tải lên: 16/02/2014, 09:20

... N P Kaimachnikov and B N Kholodenko parameters and, for clarity of exposition, we present the analysis of the Src cycle in terms of Eqns (6–8) Intrinsic regulatory properties of the Src (de)activation ... Figure 4B helps us understand this excitable behavior by presenting the pulse of Src activity in the plane of the inactive and active fractions, si and s1 If the duration of the stimulus exceeds ... large activities of Csk and RPTP, demonstrating robustness of the bistable behavior Oscillations occurring within regions and correspond to lower concentrations of active Csk and RPTP than the...

Ngày tải lên: 18/02/2014, 11:20

... Electron micrographs of a-synucleinA53T at the end of the assay confirmed the formation of fibrils, which were long (between and nm), straight and unbranched (Fig 6C,D) Addition of Gly and Lys-HCl at ... protein used, and therefore the results highlight the need to assess the activity of chaperone proteins against a variety of target proteins before drawing conclusions about their generic effects Of ... aggregation of a-lactalbumin Upon addition of 1,4-dithiothreitol (DTT), aggregation and precipitation of a-lactalbumin commenced after 25 and reached a plateau after 90 The Fig The effect of additives...

Ngày tải lên: 18/02/2014, 16:20

... these data are suggestive of a role of membranes, in particular of negatively charged membranes, in the mechanism of action of RNases, we investigated the ability of a series of RNases to destabilize ... where GR ⁄ M, GR and GM are the grid energies of the complex, of the RNase alone and of the membrane alone, respectively The following delphi parameters were used for the calculations of the grid ... similar to that of BS-RNase because of the removal of the four carboxylates The ‘basic surface’ of the mutant HHP-RNase is very similar to that of RNase-AA-GG as the presence of Glu111 is counterbalanced...

Ngày tải lên: 19/02/2014, 06:20

... of increasing concentrations of native and oxidized elafin and trappin on the activity of a constant concentration of NE and kdiss Ki ¼ kdiss ⁄ kass [5,8] Trappin is a 9.9-kDa protein formed of ... constant of enzyme inhibition by factors of three to four Thus, the deleterious effect of elafin and trappin oxidation on the affinity of the inhibitors for NE is caused by 5886 Elafin and the inhibitory ... domain of trappin each have two methionine residues (M25 and M51 for elafin, and M63 and M89 for trappin) M25 and M63 are the P1¢ residues of the inhibitors’ active centers Mass spectrometry of the...

Ngày tải lên: 19/02/2014, 07:20

... a total volume of 20 lL Reactions were stopped by the addition of 100 lL of 2· Kirby buffer [25] and 80 lL of H2O, and rRNA was obtained by precipitation after two phenol– chloroform extractions ... IMAGEQUANT software, and depurination was calculated by relating the amounts of the small aniline-fragment and 5.8S rRNA and expressing values as a percentage Reassociation and quantification of ricin ... Electron density of RTA R213D in the vicinity of residue 213 The backbone and sidechains of the R213D substitution are shown as stereo images in thick ball and stick and the position of the Arg213...

Ngày tải lên: 19/02/2014, 12:20

... presence of any of these H4-H5 loopGST ể FEBS 2004 Mapping of ATP and pNPP binding sites of the Na pump (Eur J Biochem 271) 3929 Fig Recognition of FITC by amino acids forming the ATP binding site of ... recognition of pNPP by the pNPP site at the rear surface of the N-domain is due the formation of an hydrogen bond of the NO2-group of pNPP with the amino group of N398 The hydrophobicity of the binding ... between Arg423 and Glu472 and another key residues Asp443, Ser477 and Pro489 are responsible for forming and a dierent positioning of TNP-ATP and ATP within the nucleotide binding site of Na+/ K+-ATPase...

Ngày tải lên: 19/02/2014, 16:20

... for a series of 4593 Second messenger function of cADPR A H Guse Fig Ca2+-releasing activity of some southern and northern ribose modified cADPR analogues Fig Ca2+-releasing activity of some cIDPR ... relationship of cADPR In-depth reviews covering the chemistry and biological activity of many cADPR analogues have been published [16,53–55] and the reader interested in more complete coverage of the ... Second messenger function of cADPR (and Na+) entry via TRPM2 The situation, however, appears to be more complex because physiological concentrations of AMP inhibit the effect of ADPR on TRPM2 channel...

Ngày tải lên: 20/02/2014, 01:20

... a 24 molar excess of CRI Monomers MSSAE and MSSG could not be tested as such for inhibition by CRI, because the strongly reducing conditions of the assay produce the cleavage of their mixed disuldes ... dithiothreitol [19] This hyperreactivity is a feature also of the mixed disuldes formed by Cys31 and Cys32 with glutathione [12], and of the mixed disuldes of MSSAE (unpublished results) To verify the hypothesis ... manner, and is ể FEBS 2003 Disuldes and antitumor action of BS-RNase (Eur J Biochem 270) 1983 Fig Time-course of dimerization of the labelled monomeric derivative of BS-RNase 125I-labelled MSSAE...

Ngày tải lên: 20/02/2014, 11:20

... switch and alters the expression of the two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... phosphokinase and 100 lM each of 20 L-amino acids The reaction was initiated by the addition of 200 lCiÆmL)1 of [32P]UTP (400 CiÆmmol)1) and was allowed to proceed for 45 at 33 °C Aliquots were used to ... that the transcription of CytOX I and II mRNAs in both PC12 cells and macrophages were inhibited by over 60% when cells were exposed to hypoxia for h Because each strand of the mitochondrial genome...

Ngày tải lên: 20/02/2014, 23:20

... 13G10-(Fe(ToCPP), (B) binding of two CN± ligands on the iron of 13G10-(Fe(ToCPP), (C) binding of H2O2 and imidazole on the iron of 13G10-(Fe(ToCPP), (D) binding of H2O2 and imidazole on the iron of 13G10-a,a-1,2-Fe(DoCPP) ... In¯uence of the addition of imidazole on the peroxidase activity of Fe(ToCPP), a,a-1,2- and -a,b-1,2-Fe(DoCPP) and their complexes with 13G10 Variations of the initial rate of oxidation of 0.2 ... rings packed tightly against residues of the VH domain and two pyrrole rings packed against tyrosine residues of the VL domain Binding of imidazoles to the iron(III) of Fe(ToCPP) and Fe(ToCPP))13G10...

Ngày tải lên: 21/02/2014, 03:20

... the 5¢-flanking region and determination of transcription start sites of mouse integrin a3 subunit gene The nucleotide sequence of the 0.5 kb SalI/SacI fragment and a part of exon is shown in Fig ... CCAAT box at 324 bp upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene We ... exon and approximately 4.0 kb of the 5¢-flanking region of exon of the mouse integrin a3 subunit gene We prepared a chimeric construct (pGL-ES), in which the 4.0 kb EcoRI/SacI fragment upstream of...

Ngày tải lên: 21/02/2014, 03:20

... 60% of the original activity after h, and 30% after h The D266NALP I showed 40 and 20% of the original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and ... ACKNOWLEDGEMENTS We thank Prof Takeshi Uozumi of the Department of Biotechnology, Tokyo University, for the permission to use B subtilis KN2, and Prof Koji Takahashi of the Department of Applied Biochemical ... 80% of the original activity after h The activity of N263D-Sendai decreased to 45% and 10% of the original after and h of incubation at pH 12, respectively N263D/A266Y-Sendai showed only 10% of...

Ngày tải lên: 21/02/2014, 03:20

... absence of nucleotides (Table 1) The rate of CTP synthesis was dramatically inuenced by the presence of GTP, and 20 and 41-fold increases in kcat were obtained with GTP concentrations of 0.1 mM and ... activation of the glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of UTP and ATP-cS (C) GTP activation of CTP synthesis at 0.1 mM each of UTP and ATP L ... experimental conditions used for GTP activation on the basis of the kinetic constants in Table 1, and those obtained for the glutaminase reaction in the presence of 0.1 mM each of UTP and ATP-cS (data...

Ngày tải lên: 21/02/2014, 15:20

... the use of different amounts of OS homogenates and different volumes of the fraction in the assay We con1858 firmed this observation by comparing the [3H]cGMPbinding activity of Pabc with that of ... same NaCl gradient was used [20] Comparison of the [3H]cGMP-binding activity of cone PDE with that of Pabc is discussed later Contents of cGMP in Pabcc and Pabc Pabcc and Pabc were purified from ... because of a tendency of Pabc to change its structure in Tris buffer [11] We also note that 50 lL of the peak fraction of the cGMP-nontreated Pabc contained 2.4 lg Pabc (11.6 pmol of Pabc) and...

Ngày tải lên: 06/03/2014, 00:21