reproducibility sensitivity detection limit and specificity

Báo cáo y học: " Molecular characterization of two distinct monopartite begomoviruses infecting tomato in india" pps

Ngày tải lên : 12/08/2014, 02:20

... PP, SKM, and GSS designed and conceptualized the study, PP and SM executed the experiments, PP and ARN carried out the sequence analysis, alignment and Phylogenetic studies, and PP, ARN and NRC ... sense (AV 1and AV2) and complementary strand ORFs (AC1, AC2, AC3, AC4) In depth analysis identified that at the probability of 3.26 X10-74, U38239 ToLCV-BII could be a major parent and ToLCPV ... Identification, detection and molecular characterization of novel monopartite begomoviruses Leaves of infected tomato plants were collected from two different regions of Indian subcontinent and checked...

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Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx

Ngày tải lên : 07/08/2014, 23:22

... primer set and PCR method described by Gelb et al [8] To overcome this problem, the new primer sets were designed and the nested RT-PCR was developed in order to increase the sensitivity and specificity ... Trachea Trachea Trachea Trachea and lung Trachea and lung Trachea and lung Trachea gross lesions were evaluated Gross lesions showed mildto moderated tracheitis and non-purulent airsacculitis ... region of S1 gene covering HVR I and HVR II were amplified and used for typing the field isolates in Thailand Although many different strains of live attenuated and inactivated vaccines have been...

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Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

Ngày tải lên : 18/02/2014, 08:20

... wholecell extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent ... puriﬁed proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... SGL2 and SGL3, correspond to residues 60–71, 91–99 and 151–161, respectively On the basis of the homology model, the SGLs of HYDJs and HYDBp (PDB accession number 1NFG) were superimposed and compared...

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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx

Ngày tải lên : 07/03/2014, 15:20

... deregulated in cancer cells and that environmental factors can modulate the splicing process [18,24] CRFR1 a, b, c and d isoforms differ in their ability to bind ligands and activate G proteins [10,16,25] ... reassembled by primers E21 and E23 and connected to CRFR1 (exons 1–4) by primers E3 and E23 This fragment was slightly extended by primer E27 (5¢-GGTAGTGCACCTTGCTTTTTTTCTCTCCCCA3¢) and attached to another ... primers E3 and primer E30 (5¢-AAGA ATTCTTTCCCCCCAGCCACAG-3¢) and CRFR1e with primers E3 and primer E31 (5¢-AAGAATTCTTGCT GGACCACGAACCAGGT-3¢) Final PCR fragments were puriﬁed by GFX gel band puriﬁcation...

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Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

Ngày tải lên : 21/02/2014, 00:20

... C6 and C9 aldehydes (and the corresponding alcohol) were detected in all the samples and, similarly to the pattern of LOX expression and activity, their levels were higher in stage I seed and ... start and the stop codon The introns and exons are drawn in proportion to their length (B) Nucleotide and deduced amino acidic sequences of the hazelnut LOX gene The inverted repeats are in bold and ... by RP-HPLC and then by SP-HPLC as described in Materials and methods Inset shows the retention time of the isomers produced by soybean LOX1 compared with authentic standards of 9- and 13-hydroxy...

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Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

Ngày tải lên : 07/03/2014, 15:20

... Corresponding pH optima and specific activities for syringaldazine and 2,6-DMP were pH 5.6 and 3.4 UÆmg protein)1 and pH 4.6 and 2.8 UÆmg protein)1, respectively In standard assay mixtures, the ... agarose/Tris borate/EDTA gels and appropriate bands excised and purified from the gel using the NucleoTrap Gel Extraction Kit (Clontech) The purified DNA was precipitated with ethanol and resuspended in 10 ... molecular mass standards (Bio-Rad) The isoelectric point of the enzyme was determined with the Phastsystem using PhastGel IEF 3–9 operated for 410 Vh and standard pI markers (Pharmacia) The standard assay...

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Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

Ngày tải lên : 16/03/2014, 01:20

... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... by the local ethics committee and performed according to the standards set by the Declaration of Helsinki The experiments were undertaken with the understanding and written consent of all subjects ... 18S and 28S ribosomal RNA bands after electrophoresis The RNA was quantied by spectrophotometry at 260 nm and stored at )70 C One microgram of total RNA was used to synthesize cDNA using a standard...

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Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the ﬁlamentous fungus, Talaromyces emersonii ppt

Ngày tải lên : 23/03/2014, 13:20

... 5¢- and 3¢-RACE PCRs by using the outer and inner RACE primers supplied by the manufacturer and the outer and inner gene-speciﬁc primers designed from the cel7 PCR products The Cel7 outer and ... which are stacked face-to-face and are highly curved, forming convex and concave surfaces The convex and concave sheets of the b-sandwich are composed of seven b-strands Many of the side-chains ... HEA pre-PRTLI and Enterprise Ireland awards to M.G.T C.M.C and R.T are grateful for junior teaching fellowships from NUI, Galway, and postgraduate scholarships from Enterprise Ireland References...

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Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

Ngày tải lên : 30/03/2014, 01:20

... Lys56; and aD, Ala90–Lys108); (e) two short strands 4550 of an antiparallel b-sheet (b-wing) (b2, Ser74–Ser78; and b3, Glu81–Asn85); and (f) a C-terminal loop containing a short b4-strand (His115–Asn117) ... elements labels are indicated a-Helices and b-strands are colored in red and yellow, respectively The catalytic network and the Ca2+-binding residues are indicated and shown as sticks (B) The Ca2+-binding ... Effect of temperature and pH on ChPLA2 activity (A,C) and stability (B,D) ChPLA2 ( ) and PPLA2 ( ), used as controls, were tested for activity at various temperatures (A) and pH values (C), as...

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Báo cáo sinh học: " Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

Ngày tải lên : 19/06/2014, 08:20

... and digested with EcoRV for 1.5 hrs at 37°C Unique bands generated by this restriction enzyme facilitate identification of single infections with EACMV and ACMV (lanes 1–3 and respectively) and ... generating the knowledge and improved germplasm required by scientists, breeders and farmers to combat these diseases and maximize crop yields Effective methods for sampling, storage and retrieval of ... cellular material and fix and preserve DNA and RNA within the fibre matrix [2] After a short drying period, pressed samples can be stored at room temperature for extended periods and processed when...

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báo cáo hóa học:" Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues" doc

Ngày tải lên : 20/06/2014, 04:20

... and digested with EcoRV for 1.5 hrs at 37°C Unique bands generated by this restriction enzyme facilitate identification of single infections with EACMV and ACMV (lanes 1–3 and respectively) and ... generating the knowledge and improved germplasm required by scientists, breeders and farmers to combat these diseases and maximize crop yields Effective methods for sampling, storage and retrieval of ... cellular material and fix and preserve DNA and RNA within the fibre matrix [2] After a short drying period, pressed samples can be stored at room temperature for extended periods and processed when...

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Báo cáo sinh học: "Cytogenetic and molecular characterization of eight new reciprocal translocations in the pig species. Estimation of their incidence in French populations" pot

Ngày tải lên : 14/08/2014, 13:21

... laboratory techniques used [27] G and/ or R banding is presently used systematically and allows the detection of rearrangements modifying very slightly the size and/ or the banding proﬁles of the chromosomes, ... White Candidate for reproduction rcp(5;8)(p12;q21) sire + one sister March 2000 French Landrace (FL) Candidate for reproduction rcp(15;17)(q24;q21) June 2000 Crossbred (3/4 FL; 1/4 Meishan) Candidate ... wet slides and air dried Slides were treated with 0.1% Trypsin (Difco) and stained with 3% Giemsa solution to generate GTG-banding [30] Chromosomes were arranged according to the standardized...

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IDENTIFICATION, KINETIC AND STRUCTURAL CHARACTERIZATION OF SMALL MOLECULE INHIBITORS OF ALDEHYDE DEHYDROGENASE 3A1 (ALDH3A1) AS AN ADJUVANT THERAPY FOR REVERSING CANCER CHEMO-RESISTANCE

Ngày tải lên : 24/08/2014, 09:51

... To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship has been such a help during this time Lastly, to my sweetheart Kriti Acharya, your love and support provided ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its ... mustard and its mechanism of DNA cross linking Figure represents how phosphoramide mustard forms intrastrand and interstrand cross–links between guanine bases from DNA 23 B Hypothesis and approaches...

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Expressed sequence tags analysis of major allergens producing dust mites and molecular characterization of their allergens

Ngày tải lên : 12/09/2015, 11:08

... 23-30o C and 80-90% relative humidity The house dust mites primarily feed on skin scales and dander while the storage mites feed on plants and micro-organisms (Ho and Nadchatram, 1984; Andersen ... (DC) producing IL-12 and IL-18 enhance TH1 cell development, whereas DCs expressing ICOS ligand and producing IL-10 enhance the development of TH2 and TR cells TR cells and TH2 cells develop ... showing IgE binding to DF414 and DF1096; DF414 and BT796; and BT796, BT1313 and BT1694 176 IgE binding reactivity of mites FABP homologues tested by 55 Singaporean sera and 42 Italian sera that are...

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Isolation and molecular characterization of genes associated with shoot regeneration of mustard (brassica juncea) in vitro

Ngày tải lên : 17/09/2015, 17:19

... antisense (GSTA4) and double-stranded (GST-DS1) cDNAs 112 Figure 23 Phenotype of WT and different transgenic Arabidopsis plants expressing sense (GST-S6), antisense (GST-A4) and doublestranded (GST-DS1) ... 2003) and Arabidopsis thaliana (Ikeda-Iwai et al., 2003), and androgenesis in rye (Immonen and Anttila, 1999) and triticale (Immonen and Robinson, 2000) An accumulation of H2O2 in culture is ... antisense (GST-A) and doublestranded (GST-DS) GST cDNAs 109 Figure 21 Genomic DNA gel blot analysis of transgene in transgenic Arabidopsis 111 Figure 22 Bolting and flowering of WT and different...

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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx

Ngày tải lên : 07/03/2014, 21:20

... 2B that VBARP-L and VBARP-S are coded by these specific exons VBARP-L and VBARP-S code for precursor proteins of 627 and 435 amino acids and with calculated peptide masses of 69 and 49 kDa, respectively ... were constructed and used to further confirm the Vpr and VBARP interaction using in vitro and in vivo interaction studies Ten microliters of 35S-labeled in vitro translated VBARP and Vpr or Nef ... were mixed and immunoprecipitated with VBARP-, Vpr- or Nef-specific antibody and analyzed by autoradiography (Fig 1A) Results indicate that both Vpr and VBARP were able to form a complex and the...

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Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf

Ngày tải lên : 07/08/2014, 17:22

... 2, and 3) and S parauberis (4, 5, and 6) after digestion with the restriction enzymes RsaI and AvaII, respectively M = a 100 bp ladder size marker simultaneously with group E and group P and ... and Wessman, G E Biochemical and serological characteristics of Lancefield groups E, P, and U streptococci and Streptococcus uberis Cornell Vet 1972, 62, 540-562 55 Schuman, R D., Wood, R L and ... investigated strains, and the S uberis reference strains NCDO 2038 and NCDO 2086 showed β-D-glucuronidase enzyme activity whereas isolate 138/80 and the S parauberis strains NCDO 2020 and 94/16 were...

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Identification and characterization of novel anticoagulants from bungarus fasciatus venom

Ngày tải lên : 09/09/2015, 11:08

... through binding to von Willebrand Factor (VWF) and inducing its interaction with GPIb Botrocetin is currently widely used in the detection of von Willebrand disease and GPIb-related disorders [67] ... to thrombomodulin, it changes its substrate specificity and loses its ability to cleave fibrinogen and activation of FV and FVIII Instead, it cleaves and activates protein C [82], which, in the ... to cells and plasma proteins than UFH, they have more predictable pharmacokinetics and almost 100% bioavailability after subcutaneous injection [125] Both UFH and LMWH have limitations and potential...

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Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides

Ngày tải lên : 11/09/2015, 10:02

... islands of Ireland, Iceland and New Zealand [5, 6] They have successfully colonized various habitats and feed on small animals including lizards, snakes, rodents, small mammals, birds, eggs and ... Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA sequencing; ... geographical location and environmental conditions [23-25] Chapter Figure 1.2 Snake fangs and the venom gland Cartoon representations showing the location of venom gland in the snake’s head and close look...

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Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5

Ngày tải lên : 14/09/2015, 12:44

... immunity has two main features: specificity and memory The specificity enables the adaptive immune system to recognize ‘self’ and ‘non-self’, and one particular antigen and other different antigens ... 3, 5, 7, 8, 10, 13 and 21 allergen in total IgE of S medanensis 144 Figure 6.4 C and D Correlation of IgE antibodies between Sui m 5.01 and 5.02 (C) and between Sui m 5.01 and Sui m 21 (D) 145 ... cross-inhibitions of Der f 21 and Der f Der f 21 and Der f in house dust samples 130 Discussion 132 135 Chapter 6: Identification and Characterization of Group and Group 21 in S medanensis and Cross Comparison...

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