replicative aging of yeast cells

... 600 800 1000 Number of cells 200 Number of cells Number of cells 200 100 50 0 200 400 Amount of DNA 600 800 1000 Amount of DNA (a) (b) 100 50 0 200 400 600 800 1000 Amount of DNA (c) Figure 9: ... number of cells Normalized number of cells Normalized number of cells 20 40 Time 60 0.8 0.6 0.4 0.2 80 20 40 Time (a) 60 80 Time (b) (c) Figure 12: The estimates of the time distributions of the ... analysis of the amounts of DNA in the individual cells of a cell population and on counting the number of buds of a predefined size in microscopic images The method for analyzing the amounts of DNA...

Ngày tải lên: 22/06/2014, 19:20

... from the ratio of the height of the product peptide peak versus the sum of the heights of the product peptide and substrate peaks Purification of yeast aspartyl aminopeptidase Yeast cells were grown ... position of the absorbance peak (280 nm) of the standard marker with that of aspartyl aminopeptidase Effect of inhibitors on aspartyl aminopeptidase activity Solutions of various inhibitors of enzymatic ... that the surroundings of the zinc ion in yeast aspartyl aminopeptidase differ from those of the mammalian enzyme The yeast enzyme was not affected by bestatin, an inhibitor of most aminopeptidases...

Ngày tải lên: 07/03/2014, 12:20

... Discussion reports Figure Regulation of PKA in committed cells Regulation of PKA in committed cells (a) The PKA pathway The addition of glucose to a non-fermenting yeast culture results in a rapid ... addition of nutrients The addition of either YPD or glucose to committed cells failed to induce the typical set of glucose-responsive genes In contrast, the addition of YPD (but not of glucose ... of pairwise correlations describing the similarity in the response of cells transferred to YPD at different stages of the process is shown We also compared these responses with the response of...

Ngày tải lên: 14/08/2014, 16:21

... from yeast 2.9 DNA sequencing PCR and sample preparation 2.10 Preparation of E coli competent cells 2.11 Transformation 2.11.1 Transformation of E coli by “heat shock” 2.11.2 Transformation of ... 1988) Importantly the binding of VirE2 to ssDNA forms a rigid coiled complex with each turn of the coil equalling an average of 3.4 molecules of VirE2 and 63.6 bases of ssDNA (Citovsky et al 1997) ... above all else my heartfelt thanks goes to Lynsey Table of Contents Acknowledgements Table of Contents List of Figures and Tables List of Abbreviations Summary Chapter 1, Literature review 1.1...

Ngày tải lên: 12/09/2015, 08:20

... accumulation inside the wild type and Δlas17 yeast cells 46 3.5 Detection of individual T-DNA molecules inside the yeast cells 50 3.5.1 Percentage of yeast cells with T-DNA molecule at different co-cultivation ... course of my research programme at the National University of Singapore i TABLE OF CONTENT Pages Acknowledgements i Table of contents ii Summary v List of Tables vi List of Figures vii List of Abbreviations ... MOLECULAR ANALYSIS OF THE GENE LAS17 MEDIATING T-DNA TRAFFICKING IN YEAST CELLS HARIPRIYA BATHULA (B.pharm, M.Tech) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF BIOLOGICAL...

Ngày tải lên: 13/10/2015, 16:50

... (CLP), the hepatic mRNA level of CD163 was similar with that of the sham group, which markedly decreased to approximately 7.0% of that of the CLP group (Fig 1) Table Effect of GdCl3 on serum aminotransferase ... to the inhibition of CYP activity observed in the absence of changes in protein expression in rabbit hepatocytes [22] ROS indirectly reduced the activity of selected isoforms of CYP by inducing ... role of KCs in the regulation of CYP isoform activities and gene expression profiles, partly by investigating the inflammatory signaling pathway Sham CLP GdCl GdCl3 + CLP CD163 β-actin Fig Effect of...

Ngày tải lên: 14/02/2014, 18:20

... normal cells proliferate and either replace the destroyed and damaged cells with new cells or scar tissue One characteristic feature of both proliferating cells and cancer cells is that these cells ... charge carriers and electrical properties of cells • The cells of the body are composed of matter Matter itself is composed of atoms, which are mixtures of negatively charged electrons, positively ... properties of cancer cells part • Some of the characteristic features of cancerous cells that affect their electrical activity are: Cancer cells are less efficient in their production of cellular...

Ngày tải lên: 14/02/2014, 22:20

... stimulation of ERK1 ⁄ and Pak1 pathways, gefitinib might lead to inhibition of invasiveness of human cancer cells through the inhibition of ERK1 ⁄ and Pak1 The use of gefitinib in cells with activated ... phosphorylation state of their proteins, thereby changing the amount and localization of Bcl-2 family members However, each type of cancer has its own way of disrupting the balance of the networks of signaling ... inhibition of Bax phosphorylation Regulation of Bax also occurs by mechanisms other than phosphorylation Gefitinib inhibits growth of human gallbladder adenocarcinoma cells (HAG-1) by arresting the cells...

Ngày tải lên: 16/02/2014, 09:20

... orientation of the primers Bold font represents the sequence of mature miR9* and italic fonts the sequence of mature miR9 (C) RT-PCR analysis of pri-miR9s during the neuronal differentiation of P19 cells ... for in vivo imaging of gene expression of endogenous pri-miR9 during neurogenesis To image in vivo the endogenous expression of the pri-miR9 in small animals, 2.5 · 106 of P19 cells bearing the ... FEBS M H Ko et al Bioimaging of miR9 and miR9* during neurogenesis higher endogenous level of mature miR9 than of mature miR9* In vivo visualization of unbalanced expressions of mature miR9 and miR9*...

Ngày tải lên: 18/02/2014, 17:20

... losses of activity due to absorption of enolase to the sides of the cuvette during the 45 incubation under pressure Results Exposure of yeast enolase to hydrostatic pressure in the range of 0.1–240 ... Ó FEBS 2004 Native monomers of yeast enolase produced by pressure (Eur J Biochem 271) 3899 Fig UV spectra of yeast enolase at high and low pressures The spectrum of yeast enolase, 10.6 mM, in ... interface of yeast enolase is small by the criteria of Tsai et al with only 13% of the surface buried [4] In addition, there are a large number of polar groups at the interface, many of which...

Ngày tải lên: 19/02/2014, 16:20

... Neuronal differentiation of PC12 cells by Hyper-CNTF (A) Morphology of PC12 cells grown for 48 h in serum-containing medium in the absence of factors (medium) or in the presence of 100 ngÆmL)1 CNTF, ... consequences such as stimulation of proliferation, stimulation of differentiation, prevention of differentiation, prevention of apoptosis and activation of a family of genes coding for the acute ... Hyper-IL-11 constructs First, the spectrum of target cells is more restricted All cells in the body express gp130, whereas only some cells including most cells of the nervous system express the LIF-R...

Ngày tải lên: 22/02/2014, 07:20

... corresponding peak of membrane PtdEtn-PLD (Fig 4B) Ó FEBS 2002 Fig Effect of the presence of Ca2+ during lysis on membrane and cytosolic PtdEtn-PLD activity Yeast cells were lysed in the presence of EGTA ... examine the effect of H2O2 on yeast Spo14p/Pld1p and PtdEtn-PLD activities A short exposure of yeast cells to H2O2 (30 min) caused a rapid but limited (25–30%) stimulation of PtdEtn-PLD activity ... and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ions...

Ngày tải lên: 22/02/2014, 07:20

... al [3] In a series of publications, it was shown that a single application of ultrasound could result in a dramatic loss of germ cells from testes and that this loss of germ cells was reversible ... bottom of the ring to maintain a minimum distance of cm between the bottom of the ultrasound chamber and the proximal portion of the scrotum Determining the true effective radiating area (ERA) of ... percentage of counts that fell within each range For example, if 75% of a group’s sperm counts fell in the second range of 11-20 × 10 and the remaining 25% of the counts fell in the fourth range of 41-80...

Ngày tải lên: 05/03/2014, 17:20

... exceed 3% of total cardiac output, and decrease in Pao2 with age was minimal (6 mm Hg) Most of the variance of V/Q mismatch was not a result of aging and remained unexplained; the role of an increase ... decrease in the surface area of airspace wall per unit of lung volume beginning in the third decade of life The decrease in surface area of airspace wall per unit of lung volume approximately ... of mood alterations, fatigability, lack of cooperation, or cognitive impairment Indeed, prevalence of dementia increases with aging, reaching 5.6% after age 75, 22% after age 80, and 30% as of...

Ngày tải lên: 05/03/2014, 21:20

... estimate the size of Sup35–GFP in living yeast cells After confirming that FCS was applicable to living yeast cells, fluorescence fluctuations of Sup35–GFP in [psi)] and [PSI+] cells, with or without ... with the [PSI+] cells, the flux of Sup35NM–GFP in the [psi)] cells was faster than that in the [PSI+] cells, reflecting the existence of diffuse oligomers of Sup35–GFP in the [PSI+] cells The MD-FRAP ... aggregates of Sup35 [13] The propagation of [PSI+] is strictly dependent on the presence of an appropriate amount of Hsp104 [19] Impairment of Hsp104 function, by either the deletion of Hsp104...

Ngày tải lên: 06/03/2014, 09:22

... as a regulator of the TOR pathway in yeast cells [19], the role of a4 in the mTOR-dependent control of cell growth is still unclear The yeast Tip41 protein was identified in a yeast two-hybrid ... regulator of the rapamycin-sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator of the activity of type 2A ... involved identification of interaction-deficient mutants as determined by loss of the His3+ phenotype and loss of activation of the lacZ reporter gene Subsequently, clones showing loss of interaction were...

Ngày tải lên: 07/03/2014, 05:20

... Hydroxyurea (HU) treatment of yeast cells reveals the involvement of Hat1 in the acetylation of histone H4 on Lys12 in vivo (A) Solid HU was added to exponential-phase cultures of strains W303-1a (wild-type; ... modifies Lys12 and Lys5 [13,25] Moreover, in yeast cells, indirect evidence has shown the involvement of Hat1 in the modification of Lys12, although not of Lys5, of histone H4 [26–28] However, we have ... amount of H4K12ac was found in the soluble fraction of wild-type cells after incubation with HU, but not in hat1D mutant cells (Fig 2C) H4K12ac was even detected in the soluble fraction of untreated...

Ngày tải lên: 07/03/2014, 05:20

... studies have suggested that inhibition of caspase-3 activity can block induction of apoptosis by Ab in primary neuronal cells and PC12 cells [14] The neurotoxicity of Ab seems to depend on its ability ... evaluated from the ability of cell cultures to reduce MTT, an indication of metabolic activity The assay is based on the ability of the mitochondrial dehydrogenase enzyme of viable cells to cleave the ... using PC12 cells Using a phase-contrast microscope, we observed significant morphological changes of PC12 cells treated with Ab(25–35) and caspase-3 inhibitors after 48 h (Fig 3A) In cells treated...

Ngày tải lên: 07/03/2014, 11:20

... determine the effect of a test condition on the response of PC12 cells to a stimulant, the first response of cells under test conditions was divided by the response of the cells to an identical ... response of PC12 cells to 300 lM ATP under control conditions The scaled response of PC12 cells to ATP and 2MeSATP in the various conditions studied are shown in Fig Habituation of the cells to ... solution similar to that of 30 lM ATP at the same [Mg2+] Figure (bars 6–8) shows the effect of altering the [Mg2+] on the response of PC12 cells to 60 lM 2MeSATP The response of the cells in a 0.0-mM...

Ngày tải lên: 07/03/2014, 16:20

... point mutations in the native Hsp90 of yeast render cells much more sensitive to Hsp90 inhibitor drugs To test whether mutations in the native Hsp90 chaperone of yeast influence sensitivity to Hsp90 ... therefore represent mutations in the Hsp82 isoform of yeast Hsp90 that cause partial, rather than total, loss of the essential Ó FEBS 2003 Mutations sensitizing yeast to Hsp90 inhibitor antibiotics (Eur ... sensitivities of a collection of yeast strains expressing either wild-type (p82a; w+) or mutant forms of Hsp82 (A) Strains were streaked onto YPDA agar containing the indicated concentrations of GA or...

Ngày tải lên: 07/03/2014, 21:20