remodeling of human nasal polyp epithelium

... the part of the phosphate donor that is necessary for the dimer–tetramer transi- tion of native hTK1 purified from human lymphocytes was identified. Further, the effect of the concentration of the ... slowly. Results and Discussion Identification of the group inducing tetramerization of human TK1 Human TK1 has 234 amino acids and a subunit size of 25.5 kDa [18]. Several reports have shown by ... aimed to identify the part of the nucleotide molecule that triggers tetramerization. Figure 1A–C shows the elution profiles of native TK1 from human lympho- Enzymatic regulation of human TK1 B. Munch-Petersen 572

Ngày tải lên: 18/02/2014, 12:20

... have reported the involvement of this enzyme. In the afore- mentioned system of human umbilical endothelial cells, the importance of cPLA 2 a-mediated AA release in the regulation of cell proliferation ... Reduction of U937 cell proliferation by inhibition of iPLA 2 -VIA activity was associated with arrest in G 2 ⁄ M and S phases. The iPLA 2 -VIA effects were found to be independent of the generation of ... 1915 iPLA 2 -VIA by treatment of cells with bromoenol lac- tone (BEL) [11,12]. In INS-1 insulinoma cells, acute inhibition of iPLA 2 -VIA reduces the relatively high content of lysoPC of these cells by about

Ngày tải lên: 18/02/2014, 17:20

... biochemical characterization of human ACMSD Results Cloning of ACMSD transcripts The complete coding sequence of human ACMSD was obtained from reverse-transcribed human kidney Human ACMSD RNA, by using ... recombinant isoforms Lane M, molecular mass standards production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium We found that addition of 200 ... reconstituted [21,33], catalytic activity of the human enzyme was not regained upon removal of EDTA and addition of metal-ions Figure shows the hydrophobicity profiles of human and P fluorescens ACMSD, performed

Ngày tải lên: 19/02/2014, 02:20

... expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 A B Fig Effects of hypoxia ... BSA, 30 lg of biliverdin reductase, and 15 lg of cytochrome P450 reductase After of preincubation at 37 °C, the reaction was started by addition of 10 lL of NADPH (8.4 lgỈmL) or 10 lL of water ... effect of hypoxia on the expression levels of HO-1 and HO-2 in various types of human cell line, including erythroleukemia and hepatoma cells We have shown that hypoxia reduces the expression of

Ngày tải lên: 19/02/2014, 06:20

... treat patients with stable angina pectoris, offers an in vivo model of mild myocardial ischemia in humans Irrespective of a lack of alteration in levels of other conventional cardiac markers such ... to include only the 5¢-UTR of the mRNA [15,16] However, comparison of the sequence of the 5¢-UTR of human pancreatic DNASE1 mRNA reported previously [15] with the human genomic sequence showed ... fragments of different sizes were amplified from the RNA of QGP-1 cells and pancreas (Fig 2A) Determination of the nucleotide sequences of the RT-PCR products revealed A Confirmation of utilization of

Ngày tải lên: 19/02/2014, 06:20

... small amounts of HMG-CoA and large amounts of free CoA. Peak 4 produced HMG-CoA and also large amounts of free CoA. Peak 5 produced large amounts of HMG-CoA, but also free CoA. Human 3-methylglutaconyl-CoA ... The production of (Z)-3-MG-5-CoA by Gct is probably due to the possible trans-conformation of the 5-carboxyl group of (Z)-3-methylglutaconate. Table 2. Kinetic constants of AUH (human 3-methylglutaconyl-CoA hydratase). Substrate K m (lM) V max a (UÆmg )1 ) k cat (s )1 ) k cat ⁄ ... Western blot analysis of brain extracts consistently revealed a 32 kDa AUH protein and it was thus assumed that the mature form of human AUH in brain has a molecular weight of 32 kDa (AUHp32) [15].

Ngày tải lên: 19/02/2014, 07:20

... Dobson 1 1 Department of Chemistry, University of Cambridge, UK 2 School of Biosciences, University of Nottingham, Loughborough, UK 3 School of Biology, University of Nottingham, UK Human lysozyme is ... patients. D 3 10 3 10 B A C I56T/I56V I59T V74I V93A I89V W64R S80A T70A/T70N D67H F57I 10 3 Fig. 1. Structure of human wild-type lysozyme and location of the mutations discussed in this study. The locations of the single-point mutations are shown on the structure of human wild-type lyso- zyme, ... 30-fold lower, respectively, than that of wild-type human lysozyme. More compre- hensive analysis of the secretion levels of 10 lysozyme variants shows that the low yields of these secreted proteins, under

Ngày tải lên: 19/02/2014, 08:20

... molecular mass of the disulfide isomers of HPI and the enzyme-digested fragments were measured by ESI MS. The molecular mass of the mixture of scrambled isomers was measured by MALDI-TOF MS. CD studies CD ... that of HPI and the denaturation curves are also shown in Fig. 4. Comparison of the denaturation curves of PIP with those of HPI show that PIP is significantly more stable than HPI, regardless of ... concentration of denaturant. Fig. 3. Unfolding of HPI in the presence of denaturant and thiol cata- lyst. Controls for the disulfide scrambling method include the lack of thiol catalyst and lack of denaturant

Ngày tải lên: 19/02/2014, 12:20

... absence of S-S bonds and the low number of conserved residues in this part of the sequence. Only later did the large N-terminal unit become involved in the binding of Cbl [13,14]. Assembly of two ... Specificity of monoclonal anti- (human transcobalamin) sera and their effect on the functional properties of transcobalamin. nd, not done. mAb Epitope cluster Precipitation of Apo-TC (%) Precipitation of Holo-TC ... the proximity of epitope cluster 5 and is involved in the holo-TC–receptor interaction. Fig. 4. Binding of antibodies to the proteo- lytic fragments of human TC from yeast and plants. (a) Fragments of TC y .

Ngày tải lên: 20/02/2014, 01:20

... general features of the recently determined X-ray structure of human BuChE [7,8]. In particular, most of the essential features of the catalytic site (i.e. a catalytic triad of Ser-His-Glu, an ... the mechanism of action of chymotrypsin: the role of the low barrier hydrogen bond. Biochemistry 36, 4576–4584. 46. Warshel, A. (1998) Electrostatic origin of the catalytic power of enzymes and the role of ... the limits of experimental error, K m was independent of pH for all enzymes. This is consistent with earlier reports on the pH dependence of K m for human BuChE [25,26]. The value of K ss increased

Ngày tải lên: 20/02/2014, 23:20

... hetero-oligomerization of D2L and D2S is of particular interest as it may affect the function of the two isoforms of the receptors, with possible direct consequences on the effect of antipsychotic ... observation of signal Membranes containing the equivalent of 100 fmol of receptors (as labelled with [3H]spiperone) were incubated with 2.5 nM Eu3+-labelled anti-FLAG Ig, in a total volume of 500 lL of ... protein-coupled receptors (GPCR) represent one of the largest families of genes in the human genome They are responsible for the detection of a large variety of stimuli and control many physiological

Ngày tải lên: 21/02/2014, 00:20

... inhibition of our human mutants, the role of each residue studied may be discussed taking into account previous knowledge of the biochemistry of equine aromatase [30–32] and the documented model of Graham-Lorence ... that an increase of the hydrophobic character of this part of the human active site modified the aromatization efficiency of androstenedione but increased the inhibition potency of MR 20814 and ... position of the ligand and the orientation of the hydrophobic group of V370 (modification of dihedral angles) to decrease this interaction V370 is highly conserved suggesting an important role of this

Ngày tải lên: 21/02/2014, 15:20

... the DISCOVER _3 module of INSIGHT II was used at a constant temperature of 298.0 K for 5000 steps of 1.0 fs time-step. RESULTS Model structure of human LPL All model structures of dimeric human LPL were ... for human LPL. The kit contains two kinds of LPL monoclo- nal antibody against human LPL purified from postheparin plasma. One of the monoclonal antibodies, has an epitope similar to that of the ... Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain Yoko Kobayashi, Toshiaki Nakajima and Ituro Inoue Division of Genetic Diagnosis,

Ngày tải lên: 21/02/2014, 15:20

... Njølstad 1,2 1 Department of Pediatrics, Haukeland University Hospital, Bergen, Norway 2 Department of Clinical Medicine, University of Bergen, Norway 3 Department of Biomedicine, University of Bergen, Norway 4 ... aggregation of the protein (data not shown). This instability of the pro- tein precluded an estimate of equilibrium thermody- namic parameters, and thus also measurement of the effect of ligands ... substrate (2.5 mm excess of Mg2+), saturating amounts of Glc were used In the case of. .. glucose bound to the super-open conformation Furthermore, the coordinates of human hexokinase

Ngày tải lên: 06/03/2014, 00:20

... the creation, retention and loss of inventors is an aspect of the mobility of human resources, it is a subset of the overall mobility experience in a system of innovation. Similarly, formal ... these human resources; and (4) the actual and/ or perceived match between the skills and experience of these human resources and the demands of the R&D system In terms of human ... empirical analyses of mobility in a system of innovation focus on the human resources associated with the R&D system Analysis of human resource mobility. .. the survey of causal conditions

Ngày tải lên: 06/03/2014, 05:22

... conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc- ture. The heavy chain of ... the Ser5-Arg6 bond of TNF-a at the first step, resulting in the generation of a fragment of  17 kDa. This fragment was digested to a smaller molecule of 15 kDa by scission of the Gln21-Ala22 ... After a reaction time of 47.2 h, the amount of TP41-1 at a retention time of 11 min decreased, because of frag- mentation. The fragment was observed at a retention time of 13 min (indicated by

Ngày tải lên: 06/03/2014, 22:21

... controls and nasal polyps 88 3.12.3 Comparing the impairment cilia in nasal polyps to healthy controls in vivo 89 Chapter Establishment of an in vitro culture system of human nasal ... xv List of Figures Figure 1.1 Gross view of nasal polyp Figure 1.2 Three parts of cilium 20 Figure 1.3 Ultranstructures of cilia 21 Figure 1.4 The stages of ciliogenesis ... establishment of a human nasal epithelial stem or progenitor cells (hNESPCs) model in vitro, an investigation of biophysiology and pathophysiology of cell growth and differentiation of hNESPCs isolated...

Ngày tải lên: 09/09/2015, 11:29

... reports about the number of basophils in nasal polyps in literature, suggesting a minor role of those cells in nasal polyps Secondly, in the lamina propria of inferior turbinates of allergic patients, ... directly [9] To study nasal mast cells, stimulations have been done in enzymatic dispersed nasal polyp tissue [10,11] Accessibility of nasal polyp tissue allows for easy assessment of interaction between ... Accessibility of nasal polyp tissue allows for easy assessment of interaction between different cell types in an inflammatory environment; however, the comparability of results obtained from nasal polyp...

Ngày tải lên: 11/08/2014, 08:22

... resource management and development of MISA JSC I Activities of human resource development of MISA JSC Strategies of human resource development Strategies of human resource development play an ... crystallization of intelligence and enthusiasm of youth MISA’s achievement is efforts of group of officials and staff who strive for company’s development CHAPTER Strategies of human resource ... medals, and a lot of certificate of merit from Minister of Finance, Minister of Education and Training, Minister of Information and Communication, Chairman of Accounting and Auditing of Vietnam, etc...

Ngày tải lên: 27/10/2012, 16:40

... as a fertiliser and set aside land for growth of wild plants Biological Control of Pests • This means using natural predators to eat pests instead of pesticides • It does not have harmful effects ... carbon dioxide traps heat in the atmosphere and causes the temperature of the earth to rise • This leads to disruption of the weather patterns eg drought, floods • Some weeds may thrive on the ... damages trees and pollutes rivers and lakes Acid rain causes erosion of buildings and statues particularly if they are made of limestone Deforestation • In may countries people are chopping down...

Ngày tải lên: 28/09/2013, 11:10