relevance of cell surface alterations in growth control

... crosslinking and thus more precisely indicate the site of targeting This is achieved by the fact that 4thiouridine incorporation into RNA during co-incubation in cell culture could result in thymidine ... protein (green) and GW182 (blue) GW182 proteins contain an N-terminal AGO-binding domain, which provides multiple binding sites for Argonaute proteins and a bipartite C-   28   terminal silencing ... As shown in Fig 1.2, Ago are large proteins about 100kDa comprising a single     variable N-terminal domain and three conserved C-terminal domains, including the PAZ, MID and PIWI domains (Vaucheret,...

Ngày tải lên: 09/09/2015, 10:17

... adhesion involves the interaction of leukocyte integrins of the beta subfamily (CD11a, CD11b and CD11c) with endothelial ligand receptors [19] An increase in the expression of those integrins on ... 0.04) Accordingly, a positive correlation was found between fMLP-induced ROS generation and SOFA score, with increasing values being obtained in patients with SOFA scores ≥ (Figure 5) In this study ... groups of patients and controls The expression of these integrins on neutrophils is clearly induced ex vivo upon LPS stimulation [38] In a clinical setting, Russwurm et al [20] found increased...

Ngày tải lên: 13/08/2014, 10:20

... addition of PMA to labeled cells resulted in a marked increase in the levels of a 140-kDa immunoprecipitable protein in the extracellular medium The presence of this protein in the medium of untreated ... washing the remaining cell associated label was determined and normalized to cell protein in each dish The graph represents the level of IgG-C7 binding to the cell surface as a percentage of the zero ... Furthermore, the cell surface cleavage mechanism appeared more sensitive to PMA regulation in HSF than in CHO cells Enzymes responsible for ectodomain shedding of cell surface proteins have remained largely...

Ngày tải lên: 23/03/2014, 12:20

... C-terminal sequence of OPN and the N-terminal sequence of dentin matrix acidic phosphoprotein (DMP1) [27,28] and long sequences of Glu residues in, for example, integrin-binding sialophosphoprotein ... Certain partial SP sequences have been identied as the starting point of controlled crystal growth in the extracellular matrix of mineralized tissues These include long phosphorylated sequences in, ... aS1-casein; CSN1S2, aS2-casein; CSN2, b-casein; IBSP-II, integrin-binding sialophosphoprotein II; MEPE, matrix extracellular bone phosphoglycoprotein Potential sites of phosphorylation are shown in...

Ngày tải lên: 29/03/2014, 23:20

... CXCR4 staining of noninduced Jurakt cells Panel E and G: CXCR4 staining of induced Jurkat cells The fluorescent cell in panel G is representative of a minor population of cells in the induced ... Immunofluorescence staining control with isotype-matched monoclonal antibody Panel C: CXCR4 immunofluorescence staining of H9 cells Panels E and G, CXCR4 immunofluorescence staining of HIV-1 infected ... secretory pathways In cultures productively infected with HIV1, intracellular CXCR4 staining was markedly increased in approximately 50% of the cells, with a redistribution of the staining that is consistent...

Ngày tải lên: 20/06/2014, 01:20

... problem of determining whether satE(ε) is true is coNP-complete (Theorem in [4]), and the problem of determining whether satF(ε) is true is in P (Theorem 1) □ Consider the following example of SSoD ... measuring the safetyutility tradeoff in policy inconsistency resolving Removing SSoD policies result in safety loss for the whole safety requirement in Q Similarly, Removing SA policies result in ... to organize the policy inconsistencies, so as to examine the minimum number of policies that are responsible for all the inconsistencies Definition We define a minimal inconsistency cover (MIC)...

Ngày tải lên: 20/06/2014, 22:20

... problem of determining whether satE(ε) is true is coNP-complete (Theorem in [4]), and the problem of determining whether satF(ε) is true is in P (Theorem 1) □ Consider the following example of SSoD ... measuring the safetyutility tradeoff in policy inconsistency resolving Removing SSoD policies result in safety loss for the whole safety requirement in Q Similarly, Removing SA policies result in ... to organize the policy inconsistencies, so as to examine the minimum number of policies that are responsible for all the inconsistencies Definition We define a minimal inconsistency cover (MIC)...

Ngày tải lên: 21/06/2014, 00:20

... supporting growth and factors supporting stasis The growth (or lack thereof) we observe by an individual cell is a reflection of the net sum of all growth promoting and inhibiting factors in its ... these diverse proteins include: altering activity of enzymes, altering affinity between proteins, altering affinity between protein and DNA, altering the metabolism of proteins What effect a CDK ... percentage of positive stained cells in the tumor, where: = no stain, up to 10% positive cells = light stain, 11-25% positive cells = moderate stain, 26-50% positive cells = heavy stain 51-75%...

Ngày tải lên: 09/08/2014, 03:21

... change in positive cells Shown are scatter plots of the mean change in the number of positive cells versus the difference in change in positive cells of positive cells versus the difference in change ... staining thresholds are set for primary staining (i.e CD3+ T lymphocytes), nuclear staining and background staining The output is generated in a spreadsheet as the total number of positive cells ... and within patients) and of the intraclass correlations (single rater and average of raters) Cell type Intra-observer Inter-observer Between patients Within patients ICC ICC of the mean of Between...

Ngày tải lên: 09/08/2014, 06:23

... strong predictor of adverse outcome, but other single genomic alterations, such as loss of 11q or gain of 17q, were overridden by the presence of any kind of segmental alterations in multivariate ... cases in the study of Caren et al [8] Similar to the latter findings [8], this subgroup constituted 21% of the cases in a preliminary analysis of our array-CGH data [3] These differences might in ... supported by the finding of only 2% flat genomic profiles in another study in which a tumor content of 60% had been used for sample selection [6] Because of the rare occurrence of neuroblastomas...

Ngày tải lên: 11/08/2014, 12:20

... particle -cell interactions, as mainly studied using in vitro experiments, are of a minor relevance in determining neutrophilic ROS release in vivo, i.e within the lung It also suggests that surface ... 8-OHdG induction would predominantly occur in proliferating tissue However, we did observe neither a clear contrast in visual staining intensities of individuals cells nor any difference in the ... epithelial cells (RLE) cultured in complete medium (BC) Immunocytochemical image of (B) APE/Ref-1 staining and (C) Hoechst nuclear counter-staining in RLE cells Original magnification of × 1000 Our investigations...

Ngày tải lên: 12/08/2014, 18:20

... report of the involvement of intercellular adhesion molecule-1 in syncytia formation and virus infectivity and the increase in its expression on lymphocytes in HIV infection [40] Interestingly, ... T cells in LTNP group, indicating a polarized Th1 cell immune response in LTNP group in HIV infection The CD4+ T cells of the LTNP group expressed higher levels of CD183 (CXCR3) than those of ... screening for a large number of cell surface antigens revealed that changes in the expression of activation markers were more pronounced in CD8+ T cells, whereas changes in the expression of cell...

Ngày tải lên: 13/08/2014, 05:22

... Median cell binding values are linked by solid lines; bars indicate the 25th and 75th quartile values The mean binding value of healthy controls at a single time point is represented by a dashed line ... Patients received combination antiretroviral therapy, which included: zidovudine, didanosine, stavudine, lamivudine, nevirapine, indinavir, ritonavir, nelfinavir and/or saquinavir All the patients ... responded intermittently to HAART Our hypothesis is that modulation of cell surface markers occurs during the course of HIV disease and following the initiation of HAART and these cell surface...

Ngày tải lên: 13/08/2014, 06:20

... prosthesis inserted into the portal vein and the inferior vena cava of the in vivo rabbit model Figure Diagram of silastic H-shaped prosthesis inserted into the portal vein and the inferior vena cava of ... Figure of intra-portal injection of (a) ATP and (b) adenosine on changes in hepatic arterial flow (∆ HAF) in vivo The effect The effect of intra-portal injection of (a) ATP and (b) adenosine on ... cross-clamping at point "b", distal to the point of entry of the splenic vein into the portal vein perfusate significantly inhibited the HA response to 10-8 moles 100 g liver-1 intra-arterial adenosine...

Ngày tải lên: 13/08/2014, 13:20

... correlated to markers of inflammation or hepatic function in critically ill patients In contrast to our findings (Figure 1), decreased levels of ghrelin in some rodent models of polymicrobial sepsis ... by our finding of a close inverse correlation of ghrelin with serum glucose (r = -0.369, P = 0.018) and insulin (r = -0.406, P = 0.019) as important markers of carbohydrate metabolism in non-sepsis ... ghrelin levels [18] Before promoting ghrelin as a new therapeutic target in intensive care medicine it is (in our opinion) essential to elucidate the regulation of ghrelin in human critical illness,...

Ngày tải lên: 13/08/2014, 20:22

... calculated using the index of 263 GROWTH AND MATURITY OF BARNDOOR SKATE SEXUAL MATURITY Females.—Sexual maturity in females was assessed by examining developmental changes in the gross morphology of the ... D Morin, S H Suk, and W H Howell 2003 Age and growth estimates of the Winter Skate (Leucoraja ocellata) in the western Gulf of Maine U.S National Marine Fisheries Service Fishery Bulletin 101:405– ... fishery-management tool in temperate marine systems: the Georges Bank experience Bulletin of Marine Science 66:775– 798 GROWTH AND MATURITY OF BARNDOOR SKATE Muth, K M., and D R Wolfert 1986 Changes in growth...

Ngày tải lên: 04/09/2015, 12:38

... robust insect locomotion CPG has also been used for controlling swimming robots Anguilliform swimming is one kind of swimming Its forward motion is obtained by a traveling undulation of the elongated ... UNIVERSITY OF SINGAPORE SINGAPORE 2.4 Summary 27 with this coordination connection is developed to control the walking of a bipedal robot NATIONAL UNIVERSITY OF SINGAPORE SINGAPORE 28 Chapter Coordination ... shown in the figure NATIONAL UNIVERSITY OF SINGAPORE 72 SINGAPORE LIST OF FIGURES 4.5 xiii The sensory input to the oscillator; S i, S t and S p are the inhibition input, triggering input and...

Ngày tải lên: 11/09/2015, 10:01

... and maintenance of adult HSC In addition to CXCR4, other cell- surface receptors expressed on HSC and several cell- surface adhesion molecules, including selectins and integrins, are involved in stem ... domain Moreover, all RUNX proteins have PPxY motif, a domain for the binding of WW domain-containing proteins, such as Yesassociated protein (YAP), within their transcription activation domain ... knockout status in maintaining stem cells In vitro experiments such as long term culture of stem cells showed clear synergy between loss of function of Runx1 and overexpression of EVI5, but not...

Ngày tải lên: 12/09/2015, 08:19

... 2.2.1 Growth of HCC cell lines and colon cancer cell lines…………… 41 2.2.2 Freezing HCC cell lines and colon cancer cell lines……………….42 2.2.3 Harvesting HCC cell lines and colon cancer cell lines……… ... activation of expression of insulin-like growth factor (IGF2) (d'Arville et al 1991) and insulin-like growth factor receptor (IGFR1) by HBxAg supports the growth of cells independent of other serum growth ... increased motility in the stroma, where tumor cells frequently remodel the matrix by depositing laminin In addition, the expression of fibronectin-binding integrin α5β1 often disappears in tumor and...

Ngày tải lên: 13/09/2015, 19:46

... true hyphal growth remains unclear 1.3.4 Septin ring and morphological control Besides the polarization of actin cables and patches in the budding yeast, septin ring, consisting of 10-nm filaments ... can be induced by a variety of laboratory conditions The most common hyphal inducing conditions include incubation of cells at 37°C in liquid media containing serum, N-acetyl-D-glucosamine or ... cleaving off the inhibitory C-terminal region The active N-terminal region has two functions, activating the transcription of alkaline-induced genes and repressing the transcription of acid-induced...

Ngày tải lên: 16/09/2015, 15:55