red cell membrane research

Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt

Báo cáo khoa học: The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and oxidative stress ppt

Ngày tải lên : 17/03/2014, 03:20
... was incorporated into red cell ÔghostsÕ. Its ability to protect the intrinsic membrane protein Na/K-ATPase from external stresses was studied. Red cell ghosts were created by lysing the red cells and removing ... ubiquitous to all cells, not just red cell membranes. Heat stress in NIH3T3 cells causes a transient decrease of aB-crystallin levels from the cytosol as it is translocated reversibly to the membrane ... a-Crystallin protects Na/K-ATPase in red cell ghosts (Eur. J. Biochem. 270) 2607 The molecular chaperone a-crystallin incorporated into red cell ghosts protects membrane Na/K-ATPase against glycation and...
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EU research and innovation funding – immediate changes to cut red tape for researchers and SMEs ppt

EU research and innovation funding – immediate changes to cut red tape for researchers and SMEs ppt

Ngày tải lên : 15/03/2014, 16:20
... 24 th January 2011 EU research and innovation funding – immediate changes to cut red tape for researchers and SMEs Why is simplifying research funding important? Research and Innovation ... the average). Here is an example. One category could include two researchers: researcher one with a salary of €48.000 and researcher two with a salary of €36.000. The total cost of the category ... reducing the complex array of different conditions and funding schemes that European researchers are facing. In early 2010, the Commission established a Stakeholder Platform with key EU research...
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Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

Báo cáo khoa học: Biochemical characterization of human umbilical vein endothelial cell membrane bound acetylcholinesterase ppt

Ngày tải lên : 16/03/2014, 23:20
... plasma membranes from cultured HUVECs Membranes from HUVECs were isolated and further solubilized. At different stages of this procedure, namely before and after cell lysis and after membrane solubilization, ... cellular suspension. After cell disruption, the obtained lysate was centrifuged at 47 000 g for 30 min at 4 °C in order to isolate cell membranes. When required, one more cycle of cell lysis ⁄ centrifugation was ... urogenital tract, epidermis), mesothelial cells (pleura, pericar- dium), immune cells (human leucocytes), muscle cells, endothelial cells and erythrocytes [2]. Importantly, different cellular types may express...
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Báo cáo khoa học: Integrin receptor specificity for human red cell ICAM-4 ligand doc

Báo cáo khoa học: Integrin receptor specificity for human red cell ICAM-4 ligand doc

Ngày tải lên : 23/03/2014, 13:20
... between red cell ICAM-4 and platelet a IIb b 3 was demonstrated previously [11], we provide further evidence that cell surface ICAM-4 might interact with cell surface a V b 3 . Accordingly, a cell cell adhesion ... a V b 3 L cell transfect- ants, was performed. Figure 2 shows that ICAM-4 L cells did not bind to immobilized parental L cells, but did bind to immobilized a V b 3 L cells. This cell cell adhesion ... nonadherent cells and carry out microscopic observation, as described previously [9,11]. For cell cell adhesion assays under static conditions, parental L cells and transfected a V b 3 L cells (5...
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Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21
... integral membrane protein spanning the plasma membrane once. The protein appears to be oriented with an intracellular C-terminal region of 74 residues (residues 257–330) and an extracellular ... restricted to epithelial cells. Surprisingly, the present data indicate no restriction of the MUC15 cDNA expression to epithelial cells. In contrast, expression in hematopoietic cells and tissues with function ... were collected immediately after milking and stored on ice. Milk cells were harvested by centrifugation at 3200 g for 20 min at 4 °C, and the cellular fraction was washed in NaCl/P i buffer and...
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Guidelines for Human Embryonic Stem Cell Research potx

Guidelines for Human Embryonic Stem Cell Research potx

Ngày tải lên : 06/03/2014, 15:20
... Cell Research http://www.nap.edu/catalog/11278.html 18 Guidelines for Human Embryonic Stem Cell Research THE NEED FOR GUIDELINES Since 1998, the volume of research being conducted with hES cells ... funding of research with human hES cells, and designated certain areas of hES cell research that were ineligible for NIH funding: • the derivation of hES cells from human embryos, • research in ... for use in hES cell research. Individu- als and couples who voluntarily and with full information donate somatic cells, gametes, or blastocysts for hES cell research should be assured that their...
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Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

Báo cáo Y học: Matrilysin (matrix metalloprotease-7) cleaves membrane-bound annexin II and enhances binding of tissue-type plasminogen activator to cancer cell surfaces docx

Ngày tải lên : 17/03/2014, 17:20
... cancer cells and induces notable cell aggregation, probably due to cleavage of membrane protein(s) [13,14]. However, the cleavage of annexin II by matrilysin appeared not to induce cell cell adhesion, ... shown that extracellular annexin II is involved in the regula- tion of a variety of cellular processes, including pericel- lular proteolysis, cell cell or cell ECM adhesion, and regulation of membrane ... matrilysin-cleaved annexin II from cell membranes, suggesting that annexin II binds to cell membranes via multiple receptors. Our data also suggest that the manner of annexinin II binding to cell mem- branes varies...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc

Ngày tải lên : 17/03/2014, 23:20
... leukemia and lymphoma cell lines. (A) R epre sentativ e F RET e ffi- ciency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell b asis, using flow cytometry. The cell- ind ependent intramolecular ... chains on all the four T cell types (‡ 10 5 per cell) , characteristic of leukemic or activated T cells. HLA-DR was abundant on all cell lines (‡ 5 · 10 5 copies per cell) . Surface density of ... co-clustered in the plasma membrane of human lymphoblastoid cells. Proc. Natl Acad. Sci. USA 94, 7269–7274. 39. Hwang, J., Gheber, L.A., Margolis, L. & Edidin, M. (1998) Domains in cell plasma membranes...
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Báo cáo khoa học: Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells ) implication for placental cell fusion pot

Báo cáo khoa học: Membrane trafficking of CD98 and its ligand galectin 3 in BeWo cells ) implication for placental cell fusion pot

Ngày tải lên : 23/03/2014, 09:20
... Invitrogen, Paisley, UK) per 10 6 cellsÆmL )1 cells for 30 min, or with MitoTracker Deep Red6 33 (Molecular Probes) at a concentration of 25 nm per 10 6 cellsÆmL )1 cells for 15 min; labeling was ... CDR domains, and measured its effect on cellular fusion. The cells were labeled either with DiO and Mitotracker Deep Red 633, or A B Fig. 5. Galectin 3 is detected in BeWo cells and co-immunoprecipitates ... popula- tion from cells stained either with DiO or Mitotracker red and then cultured together allows us to quantify cellular fusion ⁄ stable aggregation, which is represented by double-positive cells. To...
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Báo cáo khoa học: Detergent-resistant membrane fractions contribute to the total 1 H NMR-visible lipid signal in cell potx

Báo cáo khoa học: Detergent-resistant membrane fractions contribute to the total 1 H NMR-visible lipid signal in cell potx

Ngày tải lên : 23/03/2014, 17:21
... in other cell types and membrane fractions by 1 H NMR spectroscopy CEM-T4 cells and membranes. NMR spectra of CEM-T4 lymphoblasts displayed less mobile lipid than the stimulated Jurkat cells (CH 2 /CH 3 ratio ... DRM fractions contained about 2% of the total membrane protein content. THP-1 cells and membranes. NMR spectra of intact cells of the human monocytoid cell line THP-1, were clearly dominated by ... ngÆmL )1 ) for 24 h. Preparation and fractionation of plasma membranes Jurkat and CEM-T4 cell membranes and cytosol, prepared from 1 and 5 · 10 8 cells, respectively, were solubilized with 1% Triton...
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Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc

Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc

Ngày tải lên : 30/03/2014, 13:20
... the overproduced membrane proteins into artificial membranes could therefore be avoided, and the possibility of soluble cell- free membrane protein expression might be considered if the reconstitution ... precipitated proteins using membrane mimicking detergent micelles. Fig. 2. Cell- free production of membrane proteins. Lanes 1 and 2, in vivo expression. Samples of total cell extracts containing ... assembled human T -cell receptor by in vitro expression [37]. The cell- free expression principally offers the opportunity to insert the translated protein directly into the desired membrane of choice. Tedious...
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