... was
incorporated into redcell ÔghostsÕ. Its ability to protect the
intrinsic membrane protein Na/K-ATPase from external
stresses was studied. Redcell ghosts were created by lysing
the red cells and removing ... ubiquitous to all cells, not just red cell
membranes. Heat stress in NIH3T3 cells causes a transient
decrease of aB-crystallin levels from the cytosol as it is
translocated reversibly to the membrane ... a-Crystallin protects Na/K-ATPase in redcell ghosts (Eur. J. Biochem. 270) 2607
The molecular chaperone a-crystallin incorporated into red cell
ghosts protects membrane Na/K-ATPase against glycation
and...
... 24
th
January 2011
EU research and innovation funding – immediate
changes to cut red tape for researchers and SMEs
Why is simplifying research funding important?
Research and Innovation ... the average).
Here is an example. One category could include two researchers: researcher one
with a salary of €48.000 and researcher two with a salary of €36.000. The total cost
of the category ...
reducing the complex array of different conditions and funding schemes that
European researchers are facing. In early 2010, the Commission established a
Stakeholder Platform with key EU research...
... plasma
membranes from cultured HUVECs
Membranes from HUVECs were isolated and further
solubilized. At different stages of this procedure,
namely before and after cell lysis and after membrane
solubilization, ... cellular suspension. After cell
disruption, the obtained lysate was centrifuged at 47 000 g
for 30 min at 4 °C in order to isolate cell membranes.
When required, one more cycle of cell lysis ⁄ centrifugation
was ... urogenital
tract, epidermis), mesothelial cells (pleura, pericar-
dium), immune cells (human leucocytes), muscle cells,
endothelial cells and erythrocytes [2].
Importantly, different cellular types may express...
... between redcell ICAM-4 and
platelet a
IIb
b
3
was demonstrated previously [11], we
provide further evidence that cell surface ICAM-4 might
interact with cell surface a
V
b
3
. Accordingly, a cell cell
adhesion ... a
V
b
3
L cell transfect-
ants, was performed. Figure 2 shows that ICAM-4
L cells did not bind to immobilized parental L cells,
but did bind to immobilized a
V
b
3
L cells. This cell cell
adhesion ... nonadherent cells and carry out
microscopic observation, as described previously [9,11].
For cellcell adhesion assays under static conditions,
parental L cells and transfected a
V
b
3
L cells (5...
... integral membrane protein spanning the
plasma membrane once. The protein appears to be oriented
with an intracellular C-terminal region of 74 residues
(residues 257–330) and an extracellular ... restricted to epithelial
cells. Surprisingly, the present data indicate no restriction of
the MUC15 cDNA expression to epithelial cells. In
contrast, expression in hematopoietic cells and tissues with
function ... were collected immediately
after milking and stored on ice. Milk cells were harvested by
centrifugation at 3200 g for 20 min at 4 °C, and the cellular
fraction was washed in NaCl/P
i
buffer and...
... CellResearch
http://www.nap.edu/catalog/11278.html
18 Guidelines for Human Embryonic Stem Cell Research
THE NEED FOR GUIDELINES
Since 1998, the volume of research being conducted with hES cells ... funding of research with human hES cells, and designated certain areas of
hES cellresearch that were ineligible for NIH funding:
• the derivation of hES cells from human embryos,
• research in ... for use in hES cell research. Individu-
als and couples who voluntarily and with full information donate somatic cells,
gametes, or blastocysts for hES cellresearch should be assured that their...
... cancer cells
and induces notable cell aggregation, probably due to
cleavage of membrane protein(s) [13,14]. However, the
cleavage of annexin II by matrilysin appeared not to
induce cellcell adhesion, ... shown
that extracellular annexin II is involved in the regula-
tion of a variety of cellular processes, including pericel-
lular proteolysis, cellcell or cell ECM adhesion, and
regulation of membrane ... matrilysin-cleaved annexin II from
cell membranes, suggesting that annexin II binds to cell
membranes via multiple receptors. Our data also suggest
that the manner of annexinin II binding to cell mem-
branes varies...
... leukemia and lymphoma cell lines. (A) R epre sentativ e F RET e ffi-
ciency (E, %) histograms measured on T lymphoma/leukemia cell
lines, on cell- by -cell b asis, using flow cytometry. The cell- ind ependent
intramolecular ... chains on all the four
T cell types (‡ 10
5
per cell) , characteristic of leukemic or
activated T cells. HLA-DR was abundant on all cell lines
(‡ 5 · 10
5
copies per cell) . Surface density of ... co-clustered in the plasma
membrane of human lymphoblastoid cells. Proc. Natl Acad. Sci.
USA 94, 7269–7274.
39. Hwang, J., Gheber, L.A., Margolis, L. & Edidin, M. (1998)
Domains in cell plasma membranes...
... Invitrogen, Paisley, UK) per 10
6
cellsÆmL
)1
cells
for 30 min, or with MitoTracker Deep Red6 33 (Molecular
Probes) at a concentration of 25 nm per 10
6
cellsÆmL
)1
cells for 15 min; labeling was ... CDR domains, and measured
its effect on cellular fusion. The cells were labeled
either with DiO and Mitotracker Deep Red 633, or
A
B
Fig. 5. Galectin 3 is detected in BeWo cells
and co-immunoprecipitates ... popula-
tion from cells stained either with DiO or Mitotracker
red and then cultured together allows us to quantify
cellular fusion ⁄ stable aggregation, which is represented
by double-positive cells. To...
... in other cell types and
membrane fractions by
1
H NMR spectroscopy
CEM-T4 cells and membranes. NMR spectra of CEM-T4
lymphoblasts displayed less mobile lipid than the stimulated
Jurkat cells (CH
2
/CH
3
ratio ... DRM fractions contained about
2% of the total membrane protein content.
THP-1 cells and membranes. NMR spectra of intact cells
of the human monocytoid cell line THP-1, were clearly
dominated by ... ngÆmL
)1
) for 24 h.
Preparation and fractionation of plasma membranes
Jurkat and CEM-T4 cell membranes and cytosol, prepared
from 1 and 5 · 10
8
cells, respectively, were solubilized with
1% Triton...
... the
overproduced membrane proteins into artificial membranes
could therefore be avoided, and the possibility of soluble
cell- free membrane protein expression might be considered
if the reconstitution ... precipitated
proteins using membrane mimicking detergent micelles.
Fig. 2. Cell- free production of membrane proteins. Lanes 1 and 2,
in vivo expression. Samples of total cell extracts containing ... assembled human T -cell
receptor by in vitro expression [37]. The cell- free expression
principally offers the opportunity to insert the translated
protein directly into the desired membrane of choice.
Tedious...