red cell antibody detection and identification

... rapidly detect and identify unknown (or recombined) viruses is quite limited RAP-PCR, random RT-PCR and SISPA are complicated multi-step procedures requiring infection of cultured cells, days to ... agents Here, we present a novel method for rapid virus detection and identification based on random multiplex (RT)-PCR using 3'-locked random primers to avoid primer-dimer amplification Once ... virus amplification products can be shot-gun cloned and sequenced for identification This method may prove useful for the rapid detection and identification of emerging or recombined viruses Results...

Ngày tải lên: 18/06/2014, 18:20

... rapidly detect and identify unknown (or recombined) viruses is quite limited RAP-PCR, random RT-PCR and SISPA are complicated multi-step procedures requiring infection of cultured cells, days to ... agents Here, we present a novel method for rapid virus detection and identification based on random multiplex (RT)-PCR using 3'-locked random primers to avoid primer-dimer amplification Once ... virus amplification products can be shot-gun cloned and sequenced for identification This method may prove useful for the rapid detection and identification of emerging or recombined viruses Results...

Ngày tải lên: 20/06/2014, 01:20

... Clostridium chauvoei were analysed by culture and biochemical identification, culture and PCR and swab samples from the same muscle samples were analysed by culture and PCR The swabs were used to simulate ... suspected cases of blackleg were directly tested by PCR and compared with culture The detection by PCR gave better results than culture and biochemical identification [19], which is in disagreement with ... contributions EB and SSL designed the study and EB performed the practical analyses and writing the manuscript, with some assistance from SSL KEJ was responsible for the PCR and sequence methods, and discussed...

Ngày tải lên: 12/08/2014, 18:22

... and Pitas, 1997; Pigeon and Vandendrope 1997; Yang and Huang, 1994], feature invariant approaches [Kjeldsen and Kender, 1996; Leung, Burl and Perona 1995; McKenna, Gong and Raja, 1998; Yang and ... “divide and conquer” strategy is a means to solve this “curse of dimensionality” Face pattern detection [Li, Zhu, Zhang, Blake, Zhang and Shum, 2002; Pentland, 2000a; Pentland 2000b; Pentland and ... more and more popularity due to their simplicity and effectiveness in solving problems 2.2 Face Detection and Face Identification in a Complex Environment 12 Face Detection [Yang, Kriegman, and...

Ngày tải lên: 12/09/2015, 10:37

... magnitudes of shift in X and Y, various levels of autocorrelation of X and Y, and correlation between X and Y should be investigated The shift sizes in X and Y are set to 0, 0.5, 1, and Further, the ... the First -Detection rate of X, Y% is used to represent the First -Detection 37 rate of the variable Y, and XY% is used to represent the First -Detection rate of both X and Y The First -Detection ... network, Hotelling, MEWMA and Z charts and First -Detection rate obtained from the NN-based network and the Z chart 53 Table 4.3 ARL, SRL derived from the NN-based network and the MEWMA chart...

Ngày tải lên: 04/10/2015, 15:45

... the ghost cells was measured which was the same at t ¼ and h This indicates that the 14 C sucrose was trapped by loading and resealing and did not leak out of the ghost cells The ghost cells were ... inside red cell ghosts upon the rubidium uptake of those modifiers Error bars represent standard deviation Fig SDS/PAGE of red cell ghosts with and without a-crystallin resealed Lanes and 5, molecular ... of haemoglobin were prepared by gel filtration chromatography [18] Blood (5–6 mL) was centrifuged (1000 g) for 10 min, the plasma and white cells aspirated and the red cells resuspended at °C with...

Ngày tải lên: 17/03/2014, 03:20

... Extra -and intracellular Muc16 expression by MOVCAR cells Extra -and intracellular Muc16 expression by MOVCAR cells (A) MOVCAR-10 cells were labeled with 618F (grey line) and analyzed for cell surface ... MOVCAR-10 cells were fixed and expression of intracellular Muc16 was detected by using the 618F antibody (grey line) Dark line shows isotype control (C) Expression of cell surface and intracellular ... intracellular Muc16 and only minimal presence of Muc16 on the cell surface (Figs 6a–d) Similar results were obtained with both the 618F and 653F antibodies (Fig 6c and 6d) Discussion Figure ting Identification...

Ngày tải lên: 20/06/2014, 07:20

... was collected and used as a template Multiplex PCR for stx1, stx2, eaeA, and hlyA, and the flagellar H7 gene amplification Multiplex PCR for the detection of stx1, stx2, eaeA, and EHEC hlyA gene ... Germany) Vero cells were cultured in Eagles minimum essential medium (EMEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS) and gentamicin (100 µg/ml) Two-hundred µl of Vero cells in EMEM ... isolates by multiplex PCR for Stx1, Stx2, eaeA, and hlyA genes, and by flagellar H7 PCR After identification by motility and agglutination tests Table The detection rates of E coli O157:H7 by three...

Ngày tải lên: 07/08/2014, 18:21

... Ca, Cb and CF5 for OEA-C; Da for OEA-D; Ma and Mb for OEA-M; R and for OEA-R; F30 and F275 Hemolytic and hemagglutination techniques were used in blood-group typing (Nguyen, 1972; Nguyen and Ruffet, ... Paris and INRA from Jouy-en-Josas) Electrophoresis of transferrin and hemoglobin (Nadler et al, 1971; Bunch et al, 1978) showed that both loci were polymorphic and the analysis of red- cell blood ... Jouy-en-Josas and Lunaret) and feral ones (Corsica, Bauges, Caroux and Blood Italy) (table I) Genetic systems of transferrin and hemoglobin was detected by starch-gel electrophoresis (Nguyen and Bunch,...

Ngày tải lên: 09/08/2014, 18:21

... of red cells to determine red cell mass A second and more commonly used technique is centrifugation of a blood sample, which packs the red cells by centripetal force and leaves the plasma and ... Anemia Evaluation / Anemia and Age / 28 Anemia and Ethnicity / 38 Anemia and Pregnancy / 45 Cancer and Anemia / 58 Malaria and the Red Cell / 81 SECTION II: NUTRITION AND ANEMIA Iron Deficiency ... coat consisting of platelets and white cells The hematocrit is the fraction of the entire sample comprised by packed red cells Anemia reduces the volume of packed red cells to below normal FIGURE...

Ngày tải lên: 10/08/2014, 08:20

... phase of red cell invasion and multiplication Red cells are constantly created and then destroyed as they age and senesce A mutation that somehow destroys both the infected red cells and the parasite ... trait red cells P falciparum parasites cultured in sickle trait red cells die when the cells are incubated at low oxygen tension.15 In contrast, parasite health and growth were unimpeded in cells ... estimate of red cell size The diameter of normal red cells roughly equals that of lymphocyte nuclei Red cells generated in an iron-deficient environment often are smaller than lymphocyte nuclei and sometimes...

Ngày tải lên: 10/08/2014, 08:20

... anemia The red cells are large and have an oval shape The most striking feature is the neutrophil with seven nuclear lobes The nucleated red cell has two irregular nuclei while other cells have ... alters the CBC Impaired erythrocyte production dramatically lowers the total red cell count, hemoglobin, and hematocrit The large size CHAPTER NUTRITIONAL ANEMIAS 137 of the red cells translates ... THF Adomet methionine synthase reductase methionine synthase THF methylcobalamin Extracellular Intracellular homocysteine Cell Membrane FIGURE 8–4 Cellular uptake and metabolism of cobalamin Cobalamin...

Ngày tải lên: 10/08/2014, 08:20

... excess of 10,000 cells/μL reduce the risk of catastrophic bleeds, such as intracranial hemorrhage ᭿ PURE RED CELL APLASIA Pure red cell aplasia reflects selective destruction of red cell progenitors ... versus TEC • Anemia • Normal neutrophil and platelet counts • Normochromic red cells in TEC; macrocytic red cells in DBA • Elevated Hb F in DBA • Elevated red cell ADA in DBA Fanconi’s anemia versus ... androgen therapy include masculinization, severe acne, and hepatic damage (hepatic peliosis and liver tumors) Supportive treatment with red cell and platelet transfusions then is often required...

Ngày tải lên: 10/08/2014, 08:20

... Coproporphyrinogen oxidase activity and porphyrin concentrations in peripheral red blood cells in hereditary sideroblastic anaemia Scand J Haematol 34:235–237 238 STEM CELL DYSFUNCTION 81 82 83 84 ... Disease.”11 This virus invades early red cell precursors in the bone marrow, essentially shutting off red cell production Because of the only 10–15 days survival of Hb SS red cells, profound anemia develops ... syndrome Sickle cell disease in children also produces significant systemic problems The chronic and vigorous production and destruction of red cells creates a tremendous energy demand These children...

Ngày tải lên: 10/08/2014, 08:20

... spectrin and the intrinsic membrane protein, band Actin and protein 4.2 are also prominent members of the red cell cytoskeleton (From Lux SE, Palek J 1995 Disorders of the red cell membrane In: Handin ... normal and HS red cells The upper row of the diagram highlights the cytoskeleton in the cells, showing the reduction associated with HS The middle row shows that both normal and HS red cells initially ... biophysical and biochemical properties of red cell membranes and promote hemolysis As Figure 15-2 shows, glutathione peroxidase defuses H2 O2 before it can wreck havoc on the cell, a job shared with...

Ngày tải lên: 10/08/2014, 08:20

... introduction of red cells that not have the target antigens for antibody (2) Antibody (3) can be introduced into the circulation if plasma accompanies the transfused blood cells The antibody (an alloantibody) ... DYSFUNCTION IgG Autoantibody SECTION VIII DAT Antibody Agglutinated Red Cells FIGURE 19–2 Schematic representation of the DAT The thoroughly washed red cells in the figure have only IgG autoantibody attached ... or red cell sensitization.10 The drug induces red cell autoantibodies in 10–20% of patients after or more months of use These red cell antibodies are true autoantibodies directed against red...

Ngày tải lên: 10/08/2014, 08:20

... the BV-2 and RAW 264.7 cell lines was performed These two cell cultures were infected with a same MNV-1 stock and incubated for 48 hours, with samples of both intracellular and extracellular ... this laboratory In brief, cells were seeded into 6-well plates at a rate of ~7.5 × 104 cells/well for the BV-2 cell line and ~5.5 × 105 cells/well for the RAW 264.7 cell line, densities that ... All liquid was removed from the plates and cells were covered with mL/well of a 1.5% methylcellulose overlay medium After three days, cells were fixed and stained with mL/ well of crystal violet-formalin...

Ngày tải lên: 12/08/2014, 04:20

Ngày tải lên: 13/11/2014, 10:36

... as compared to control cells Secondary replating assay was used to compare the extent of differentiation between Lefty2 RNAi cells and control cells a) Two seeding densities (300 and 500 cells) ... specifically that the enzymes and cellular machinery required to process Lefty2 are present in ES cells and assures us that Lefty2 processing is indeed active in ES cells and that mature Lefty2 proteins ... triplicates (n=3) and the cell line used is E14 49 2.1.2.2 Lefty2 shRNAs reduce Lefty2 expression level at both the transcript and protein level ES cells will differentiate, or perhaps undergo cell death...

Ngày tải lên: 11/09/2015, 16:02

... maintenance of ES cell self-renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates were shortlisted An RNAi screen in mouse ES cells (E14) using Oct4 and Nanog promoter ... understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, BMP and WNT pathways and that of the core transcriptional regulatory circuitry governed by OCT4, SOX2 and ... specification of ES cells is a SMAD3 dependent process On the other hand, SMAD3 plays no role in trophectodermal 158 specification of ES cells as inhibition of SMAD3 activity does not lead to ES cell differentiation...

Ngày tải lên: 11/09/2015, 16:02