... of haemoglobin were prepared by gel filtration chromatography [18] Blood (5–6 mL) was centrifuged (1000 g) for 10 min, the plasma and white cells aspirated and the red cells resuspended at °C with ... the ghost cells was measured which was the same at t ¼ and h This indicates that the 14 C sucrose was trapped by loading and resealing and did not leak out of the ghost cells The ghost cells were ... inside red cell ghosts upon the rubidium uptake of those modifiers Error bars represent standard deviation Fig SDS/PAGE of red cell ghosts with and without a-crystallin resealed Lanes and 5, molecular...
Ngày tải lên: 17/03/2014, 03:20
... Ca, Cb and CF5 for OEA-C; Da for OEA-D; Ma and Mb for OEA-M; R and for OEA-R; F30 and F275 Hemolytic and hemagglutination techniques were used in blood-group typing (Nguyen, 1972; Nguyen and Ruffet, ... Paris and INRA from Jouy-en-Josas) Electrophoresis of transferrin and hemoglobin (Nadler et al, 1971; Bunch et al, 1978) showed that both loci were polymorphic and the analysis of red- cell blood ... Jouy-en-Josas and Lunaret) and feral ones (Corsica, Bauges, Caroux and Blood Italy) (table I) Genetic systems of transferrin and hemoglobin was detected by starch-gel electrophoresis (Nguyen and Bunch,...
Ngày tải lên: 09/08/2014, 18:21
ANEMIAS AND OTHER RED CELL DISORDERS - PART 1 docx
... of red cells to determine red cell mass A second and more commonly used technique is centrifugation of a blood sample, which packs the red cells by centripetal force and leaves the plasma and white ... buffy coat consisting of platelets and white cells The hematocrit is the fraction of the entire sample comprised by packed red cells Anemia reduces the volume of packed red cells to below normal ... information on white cells and platelets in the blood sample Consideration of these cells is central to the evaluation of anemia Anemia in association with a low white cell count and low platelet count...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 3 ppt
... phase of red cell invasion and multiplication Red cells are constantly created and then destroyed as they age and senesce A mutation that somehow destroys both the infected red cells and the parasite ... trait red cells P falciparum parasites cultured in sickle trait red cells die when the cells are incubated at low oxygen tension.15 In contrast, parasite health and growth were unimpeded in cells ... estimate of red cell size The diameter of normal red cells roughly equals that of lymphocyte nuclei Red cells generated in an iron-deficient environment often are smaller than lymphocyte nuclei and sometimes...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 4 pot
... anemia The red cells are large and have an oval shape The most striking feature is the neutrophil with seven nuclear lobes The nucleated red cell has two irregular nuclei while other cells have ... THF Adomet methionine synthase reductase methionine synthase THF methylcobalamin Extracellular Intracellular homocysteine Cell Membrane FIGURE 8–4 Cellular uptake and metabolism of cobalamin Cobalamin ... intestine and colon, but occasionally extends to the jejunum and duodenum Invasion of the submucosa by inflammatory cells and disruption of tissue architecture impair absorption both of iron and dietary...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 6 pps
... androgen therapy include masculinization, severe acne, and hepatic damage (hepatic peliosis and liver tumors) Supportive treatment with red cell and platelet transfusions then is often required ... versus TEC • Anemia • Normal neutrophil and platelet counts • Normochromic red cells in TEC; macrocytic red cells in DBA • Elevated Hb F in DBA • Elevated red cell ADA in DBA Fanconi’s anemia versus ... excess of 10,000 cells/μL reduce the risk of catastrophic bleeds, such as intracranial hemorrhage PURE RED CELL APLASIA Pure red cell aplasia reflects selective destruction of red cell progenitors...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 7 doc
... Coproporphyrinogen oxidase activity and porphyrin concentrations in peripheral red blood cells in hereditary sideroblastic anaemia Scand J Haematol 34:235–237 238 STEM CELL DYSFUNCTION 81 82 83 84 ... Disease.”11 This virus invades early red cell precursors in the bone marrow, essentially shutting off red cell production Because of the only 10–15 days survival of Hb SS red cells, profound anemia develops ... syndrome Sickle cell disease in children also produces significant systemic problems The chronic and vigorous production and destruction of red cells creates a tremendous energy demand These children...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 9 ppt
... spectrin and the intrinsic membrane protein, band Actin and protein 4.2 are also prominent members of the red cell cytoskeleton (From Lux SE, Palek J 1995 Disorders of the red cell membrane In: Handin ... normal and HS red cells The upper row of the diagram highlights the cytoskeleton in the cells, showing the reduction associated with HS The middle row shows that both normal and HS red cells initially ... biophysical and biochemical properties of red cell membranes and promote hemolysis As Figure 15-2 shows, glutathione peroxidase defuses H2 O2 before it can wreck havoc on the cell, a job shared with...
Ngày tải lên: 10/08/2014, 08:20
ANEMIAS AND OTHER RED CELL DISORDERS - PART 10 docx
... other red cell antigens of the Rh system including C, c, and e Antigens outside the Rh family such as Kell, Duffy, Kidd, and S/s on occasion produce similar problems if the mother lacks these antigens ... or red cell sensitization.10 The drug induces red cell autoantibodies in 10–20% of patients after or more months of use These red cell antibodies are true autoantibodies directed against red ... infection, 188–189 red cell distribution width (RDW) value, 11 red cell fragmentation syndrome, 59 red cell membrane cytoskeleton, 332, 332f refractory anemia, 211 cytopenia, peripheral blood cell line...
Ngày tải lên: 10/08/2014, 08:20
Báo cáo khoa học: Integrin receptor specificity for human red cell ICAM-4 ligand doc
... nonadherent cells and carry out microscopic observation, as described previously [9,11] For cell cell adhesion assays under static conditions, parental L cells and transfected aVb3 L cells (5 · ... with cell surface aVb3 Accordingly, a cell cell adhesion assay using ICAM-4 and aVb3 L cell transfectants, was performed Figure shows that ICAM-4 L cells did not bind to immobilized parental L cells, ... Adhesion of LAD cells to ICAM-4-Fc Fig Adhesion of ICAM-4 L cell transfectant to immobilized aVb3 L cell transfectant ICAM-4 L cells (black bars) or parental L cells (white bar) (105 cellsÆwell)1)...
Ngày tải lên: 23/03/2014, 13:20
báo cáo khoa học: "Types of red cell potassium in seven of cattle Spanish native breeds" pdf
... frequencies and BE AKAJIMA ISHI AMENAKI membrane properties of high potassium type red cells in cattle and goats Jap J Zootech Sci., 51, 215-222 ANDEY P M.D., Ro A., 1968 Potassium and sodium ... (Kwb) and plasma (Kp) were estimated by flame VANS + photometry using the method described by E (1954) Red- cell K concentration was calculated indirectly, by means of the formula : III Results and ... populations of up to 46, 49 and 52 m-equiv/1 and grouping them into classes of m-equiv/ From the results obtained in this study, we propose that it be + set at 46 m-equiv/ of red cell K populations were...
Ngày tải lên: 09/08/2014, 22:22
Báo cáo khoa học: "Endocrine Measurements and Calving Performance of Swedish Red and White and Swedish Holstein Dairy Cattle with Special Respect to Stillbirth" ppsx
... pregnant dairy heifers and cows (6-7 months of pregnancy) were used They were 51 Swedish Red and White (SRB) and 38 Swedish Holstein (SLB) breeds In these numbers, animals (7 SRB and SLB) were examined ... sulphate and progesterone concentrations in cows and ewes associated with fetal death and abortion Br Vet J 1987, 143, 238245 Noakes DE, Parkinson TJ, England GCW: Arthur's Veterinary Reproduction and ... stillbirth and associated factors in Swedish cattle breeds Acta Agri Scand 1976, 26, 151-164 Philipsson J: Strategies to reduce problems in calving performance and stillbirths by selection and differential...
Ngày tải lên: 12/08/2014, 15:20
Báo cáo y học: "Variation in red cell transfusion practice in the intensive care unit: a multicentre cohort study" pptx
... significant institutional practice variation and reasons why red cells are administered in critically ill patients Methods Study design We implemented two concurrent and complementary data gathering approaches ... categories, the number of red cell units administered per patient (0 units, 1–3 units, 4–6 units, 7–9 units, 10 or more units) and mortality rates were initially compared amongst institutions ... lowest concentrations overall and patients who received red cells [AU: Change to sentence OK?] The influence of APACHE II scores appeared least important in centres (3 and 6) with a conservative...
Ngày tải lên: 12/08/2014, 18:20
Báo cáo khoa học: " Energy balance, leptin, NEFA and IGF-I plasma concentrations and resumption of post partum ovarian activity in swedish red and white breed cows" potx
... fat, protein and lactose Page of (page number not for citation purposes) Acta Veterinaria Scandinavica 2008, 50:3 http://www.actavetscand.com/content/50/1/3 Table 1: Ingredients and nutrient ... NaHeparin and heparinised blood was immediately centrifuged for 20 minutes at 1000 × g Separated plasma was transferred in plastic tubes and stored at -20°C for leptin, NEFA, IGF-I and progesterone ... milk production and milk protein and fat content of cows, average EB values and weekly mean dry matter intake, respectively of group A and B Average milk production of group A and B during seven...
Ngày tải lên: 12/08/2014, 18:22
Báo cáo y học: " Red cell transfusion triggers in critically ill patients: time for some new TRICCs" docx
... associated with small (1% absolute risk reduction) but significant ICU mortality reduction in Canada, and may be particularly important with prolonged red cell storage [14,15] Should we use blood ... Current issues relating to the transfusion of stored red blood cells Vox Sanguinis 2009, 96:93-103 doi:10.1186/cc9043 Cite this article as: Walsh TS: Red cell transfusion triggers in critically ill ... guideline: red blood cell transfusion in adult trauma and critical care Crit Care Med 2009, 37:3124-3157 12 Hill SR, Carless PA, Henry DA, Carson JL,Hebert PC, Henderson KM: Transfusion thresholds and...
Ngày tải lên: 13/08/2014, 20:22
Hull and White model
... u,KT1 e2K v 2v dv du: t t The variances and covariances are not random The means are random through a dependence on rt Advantages of the Hull & White model: Leads to closed-form pricing formulas ... this is random only through a dependence on rt To compute R this option price, we need the joint distribution of tT1 ru du; rT1 conditioned on rt This CHAPTER 30 Hull and White model ... derivatives Consider, for example, f x = 8x IR; g x = sin1000x 8x IR: 100 CHAPTER 30 Hull and White model 299 Then jf x , gxj 100 8x IR; but because g 0x = 10 cos1000x; we have jf...
Ngày tải lên: 18/10/2013, 03:20
The JSP Files (Part 3) - Black Light and White Rabbits
... (part 3): Black Light And White Rabbits And the output is: The string black light starts with bl The string white rabbit ends with it Screaming Out Loud 13 You Say Seven, I Say And finally, in case ... Black Light And White Rabbits // define variables String alpha = "black light"; String beta = "white rabbit"; String prefix = "bl"; String suffix = "it"; // check each string for prefixes and suffixes ... someString has been converted to a number, stored in someNumber out.println(someNumber + " plus " + aRandomNumber + " equals " + (someNumber+aRandomNumber)); %> And here's the output 97 plus equals 973...
Ngày tải lên: 23/10/2013, 00:15
Black Box and White Box testing
... values a and b, test cases should include a and b, values just above and just below a and b If an input condition specifies and number of values, test cases should be exercise the minimum and maximum ... of stubs, drivers and test object libraries Development and use of standard procedures, naming conventions and libraries Establishment and maintenance of regression test suites and procedures Allocation ... this system to understand and clarify the characteristics of black box and white box testing People: Who does the testing? Some people know how software works (developers) and others just use it...
Ngày tải lên: 25/10/2013, 03:20
Tài liệu Black and White in Photoshop CS3 and Photoshop Lightroom P2 docx
... creating neutral values in producing a black and white prints with desktop 18 Color Management for Black and White printers (See Phase “Print Profiling and Printer Settings”, page 25 for more information.) ... specified predefined color management settings, Photoshop can proceed within the user defined color management workflow as standard protocol for all documents and color data that you 21 Black and White ... on screen, and how Photoshop operates color separations These profiles are known as working spaces Being aware of your color settings and image 23 Black and White in Photoshop CS3 and Photoshop...
Ngày tải lên: 19/01/2014, 22:20
Tài liệu Black and White in Photoshop CS3 and Photoshop Lightroom P1 pptx
... Color Mode 101 Color Filters and Black and White Images 103 Background: Color Filters for Black and White Film 104 How Color Filters in Black and White Made Color Film 106 Channels ... image, and the same in black and white can become mesmerizing xix Seeing in Black and White Look for silhouettes A striking outline of a backlit object always appears interesting, and in a black and ... “look better in black and white Combine a macro lens with good, strong texture and see how much better that color image looks in black and white! Look for lines Lines can break and bisect an image,...
Ngày tải lên: 19/01/2014, 22:20