recombinant protein from live animals and plants

... digestion of purified apoprotein with trypsin (T) and Asp-N endoproteinase (D), and of native protein with Glu-C endoproteinase (S) Amino acids identified by Edman degradation and MS tandem fragmentation ... automated Edman degradation and tandem MS of peptides generated by different digestions on apoprotein and native protein (Table 1) GHP contains 72 amino-acid residues, and the measured mass (Fig ... mode with horse cytochrome c as internal calibrant Peptides from the tryptic and Glu-C endoproteinase digestion on apoprotein and native protein, respectively, were separated on a Pep-S II C2 ⁄...

Ngày tải lên: 08/03/2014, 08:20

... are several disadvantages and challenges which could suffer from when using animal cell in the production of recombinant proteins  Due to low yield and complicated and costly cultivation, therefore ... production of recombinant proteins due to following reasons:  They could process authentic post-translational modifications of recombinant proteins, which bacteria not carry out (Primrose and Twyman, ... be the most widely adopted and utilized for producing recombinant proteins commercially (Bertschinger et al., 2008) Regarding recombinant CHO cell lines, efficiency of protein production is dependent...

Ngày tải lên: 28/03/2014, 09:20

... Kiến thức Kỹ  Còn nhiều lỗi Tiết Unit 26 Animals and plans  Sạch  Đẹp  Làm hết  Nhanh (so tập với nhóm) chưa nhanh  Giải thích TiÕt Unit 26 Animals and plans ... the sentences: a Mr Hai is a _(1) b He has _(2) _(3) and produces a lot of rice c Mr Hai has some _(4) They plow the fields and pull a cart d The cows produce a _(5) _(6) e The chickens ... produce a _(7) _(8) _(9) Answer Act I: farmer some fields buffalo little Tiết Unit 26 Animals and plans milk lot of eggs Act II/ Make questions for the answers: a Mr Hai produces a lot of...

Ngày tải lên: 14/08/2014, 11:20

... We are _ plants and animals The Asian animals are in _ Anwser: 1.growing 2.food/land Tiết Unit 16 - Animals and plants 3.cuting 4.because 5.danger Tiết Unit 16 - Animals and plants 6.destroying ... Famers are burning the forests Plants and wild animals are not in danger Anwser: 1.T 2.F (air -> land/food) 3.F (making -> cutting) Tiết Unit 16 - Animals and plants 4.T 5.F (are not in -> are ... gian (không làm hết tập) Tiết Unit 16 - Animals and plants Kịp thời gian (hoàn Nhanh (so với thời thành tập) gian cho phép) TiÕt Unit 16 - Animals and plants ...

Ngày tải lên: 14/08/2014, 11:20

... Spines and thorns can hurt animals Animals not want to eat these plants Other plants have a bad taste or smell That keeps animals away too Some plants use camouflage like animals Some plants ... They use their claws and teeth Zebras eat grass Their flat teeth help them bite and chew Hawk Animals Stay Safe Animals have different ways to stay safe The colors and shapes of animals can protect ... Habitats Plants live in different places Plants need light from the Sun to grow This plant lives in a dark rain forest The big leaves can take in lots of light for the plant Heliconia 12 Plants...

Ngày tải lên: 05/04/2016, 12:12

... Pi-injected and dsRNA-injected females Lanes 1–3: Ovary from the corresponding NaCl ⁄ Pi-injected individual and 4–6 dsRNA-injected animals Each lane represents protein samples collected from individuals, ... injection (Figs 4A and 5A) In conclusion, the use of recombinant protein or RNAi alone may not be sufficient to confirm the function of a neuropeptide The combined use of recombinant protein and RNAi described ... level in control (–) and dsRNA-injected (+) females from animals at different time intervals (i.e 0, 24, 48, 72, 96 and 120 h); the arrow indicates the MIH-B transcript, and the smear indicates...

Ngày tải lên: 16/03/2014, 05:20

... type-1 and type-3 RIPs For example, tobacco plants expressing high levels (>10 ngÆmg protein) 1) of the type-1 RIP from Phytolacca americana exhibited a stunted, mottled phenotype, and the plants ... (Asp26, Gln39, Gly41, Asn48 and Gln49 for 1a, and Asp231, Ile243, Tyr245, Asn252 and Gln253 for c) of the binding sites O and N atoms of the amino acids are coloured white and black, respectively ... the A- and B-chains of SNA-If and SNA-I Comparison of the molecular structure and biological activities of SNA-I and SNA-If To check whether the differences in sequence affect the structure and/ or...

Ngày tải lên: 31/03/2014, 23:20

... values from duplicate determinations from a single experiment (data for 0.1, and 10 nM) and the mean ± SD from three independent experiments each with duplicate determinations (data for 0.5 and ... previously for human and bovine recombinant protein C [14] with some modifications Serum-free conditioned medium (5 L) from stably transfected cells containing each recombinant mouse protein C was supplemented ... sequence alignment from different species and mouse protein C variants used in the present study N-terminal Gla sequence (1–44) is shown and defined between the propeptidase and chymotrypsin cleavage...

Ngày tải lên: 18/02/2014, 06:20

... were purchased from Sigma cAMP and dithiothreitol were from Fluka The Fractogel EMD SO3 650 (M) was from Merck, and Q-Sepharose Fast Flow, Sephacryl S-200 HR and Sephadex G-25 were from Amersham ... cAMP receptor protein from Escherichia coli with cAMP and DNA studied by dynamic light scattering and timeresolved uorescence anisotropy methods J Protein Chem 20, 601610 31 Sứgaard-Andersen, L., ... was calculated from the equation: QD ẳ QRF SD ARF SRF AD 1ị where SD and SRF are the respective areas under the emission spectra of the donor and a reference compound, and ARF and AD are the...

Ngày tải lên: 20/02/2014, 23:20

... regarding global shape and dimensions, and differs from data on other invertebrate carotenoproteins such as the lobster crusta˚ cyanin (a cylinder of 238 · 95 A) [21] and the starfish linckiacyanin ... invertebrate carotenoproteins for which a 3D structure has been resolved, and a comprehensive body of information on the protein is available [11,13,19–24] It is evident from these studies that ... holoovorubin and apo-ovorubin normalized for protein concentration Clearly, the two curves virtually overlap, indicating that both ovorubin forms have nearly the same shape and size From the Guinier...

Ngày tải lên: 07/03/2014, 06:20

... unclear and no definitive cure for the disease is available Prolidase is widespread in nature and has been isolated from mammals [2], bacteria, such as Lactobacillus [18] and Xanthomonas [19], and from ... studies and developing strategies for enzyme replacement therapy for PD Results Recombinant human prolidase expression and purification from eukaryotic and prokaryotic sources Total cellular RNA from ... profile of recombinant prolidase obtained from CHO (A) and E coli (C) j, prolidase activity; r, gradient of imidazole (B, D) Western blotting of recombinant prolidase obtained from CHO (B) and E coli...

Ngày tải lên: 07/03/2014, 11:20

... formation depends upon contact of b-strand 10 from the C-terminal extensions of monomers A and D with b-strands and in the a-crystallin domain of monomers C and B, respectively (Fig 8) A more prominent ... activity decreased from R110G to Y116D, which were similar, and then to F112R Chaperone activities of p26 from Artemia [45] and E coli with citrate synthase were similar, whereas BSA and IgG at 1200 ... dimers, with monomers A and B in dimer and C and D in dimer (Fig 8) The a-crystallin domain of each monomer is composed of nine b-strands (labeled b2–b10), with the b6 strand situated in a large...

Ngày tải lên: 07/03/2014, 12:20

... obtained from Molecular Probes (Eugene, OR) The nutrients for bacterial growth were from Gibco BRL KCl, NaCl, NaH2PO4, and KI were obtained either from Fluka (Buchs, Switzerland) or from Riedel-deHaen ... binding sites involves a sequence of protein conformational changes, which shift the protein from a low-afnity nonspecic DNA-binding protein to a state of the protein that binds DNA with FEBS Journal ... DNA and cAMP i.e free CRP, CRP(cAMP)2 and CRP(cAMP)4 In the presence of % 100 lm cAMP, the protein becomes activated by the formation of a CRP(cAMP)2 complex and it is then able to recognize and...

Ngày tải lên: 07/03/2014, 16:20

... CD spectra of recombinant pulchellin A-chain (rPAC), recombinant pulchellin B-chain (rPBC), recombinant pulchellin (rPAB) and native pulchellin Spectra were obtained from each protein at a concentration ... toxin, similar to abrin and ricin Cloning of the coding gene from pulchellin A-chain will greatly facilitate the understanding of the protein structure and function, and lay a solid foundation ... abrin-a, abrin-c and ricin are composed of ve invariant residues (Tyr74, Tyr113, Glu164, Arg167 and Trp198 in abrin-a and abrin-c, and Tyr115, Tyr158, Glu212, Arg215 and Trp246 in ricin) and another...

Ngày tải lên: 07/03/2014, 16:20

... Rosetta(DE3)pLysS as wild-type protein wt-Fdmor, as N-terminal His-tag fusion protein NHis-Fdmor and as C-terminal His-tag fusion protein CHis-Fdmor All proteins were soluble and no inclusion bodies ... the purified recombinant Fdmor variants (A) and purified recombinant P450mor (B) (A) Lane 1, marker proteins; lane 2, wt-Fdmor; lane 3, NHis-Fdmor; lane 4, CHis-Fdmor; lane 5, marker proteins (B) ... agreement with those predicted from the sequences (Table 1) Absorption spectra were the same for all three recombinant proteins, containing only a single peak at 412 nm, and the protein peak at 280 nm...

Ngày tải lên: 07/03/2014, 16:20

... was named bapA and its deduced amino acid sequence showed sequence similarity to the DmpA from O anthropi LMG7991 The bapA gene was expressed in an E coli host and the recombinant protein (BapA) ... pRTB1-Fba and pRTB1-Pst and structural organization of the 6668-bp DNA containing ORF1, ORF2 (bapA), ORF3, ORF4, ramA, ORF6 and ORF7 from Pseudomonas sp MCI3434 The location of the ORFs and the ... FEBS 3077 b-Ala-Xaa dipeptidase from Pseudomonas sp H Komeda and Y Asano Fig Comparison of the amino acid sequences of BapA and homologous proteins Identical and conserved amino acids among the...

Ngày tải lên: 07/03/2014, 21:20

... (lanes and 5), the sediments of 10 000 g (lanes and 6) and 100 000 g (lanes and 7) centrifugation and a soluble fraction (lanes and 8) were prepared as described under Materials and methods, and ... between lunasin and p16, different genes may be employed along evolution for similar functions and plants offer many examples in this line Ó FEBS 2002 H3 and H4-binding chromatin protein from pea seeds ... between p16 and histones probed by Far Western blotting Core histones from chicken erythrocytes (lanes c) and from pea (lanes p) were separated by SDS/PAGE (A) shows a Coomassiestained gel and (B)...

Ngày tải lên: 08/03/2014, 10:20

... performed and the protein bands were silver stained as described in Materials and methods (B) Isoelectric focusing of the purified lamjapin Lane L, lamjapin (4 lg); M, protein markers Proteins ... total proteins and also to the low efficiency of extraction Physical properties of lamjapin Lamjapin obtained from the Mono Q column migrated as a single band characterized by both 12% SDS/PAGE and ... chromatography was chosen as the first step and it separated efficiently the target proteins from the inhibitory substances (Table 1) Proteins of peak from the phenyl-Sepharose CL-4B were then subjected...

Ngày tải lên: 08/03/2014, 10:20

... carboxamidomethylation and tryptic digestion of HCII from plasma and from recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and digested with trypsin; ... serum-derived and recombinant HCII Natural and recombinant HCII were characterized further by peptide mapping After reduction and carboxamidomethylation, the proteins were digested with trypsin and the ... to the manufacturer’s protocol (Novex), and subsequent visualization of proteins by Coomassie blue staining The homogeneity of HCII from plasma and from recombinant CHO cells was checked by N-terminal...

Ngày tải lên: 08/03/2014, 22:20

... form of each S100 protein, except for MRP126 and ictacalcin, which have only been isolated from chicken and catfish, respectively The number of identities between each S100 protein and calhepatin ... dimerization and monomer stability, in Sl00 A4 and other S100 proteins [23] (Fig 3), are clustered between helix I and IV (Fig 5B), in the same way as in Sl00 A4 and other S100 proteins [23] ... crystal and/ or NMR structures of other members of the family The molecule is depicted in strand representation, and a helices are numbered from I to IV (B) Residues Leu7, Arg8 and Phe11 from a-helix...

Ngày tải lên: 08/03/2014, 22:20