receptors active sites and cell signaling clusters
báo cáo hóa học:" Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cells" potx
... induces Th1 cell and macrophage cytokines and generates LAK cells in synergy with rIL-2 Planta Med 1998, 64:110-115 Viola A, Luster AD: Chemokines and their receptors: drug targets in immunity and inflammation ... biomolecular assays and data interpretation JL, JR and GC participated in study design and interpretation of results AL designed the study and led the data interpretation and manuscript writing ... injection The injection volume was 15 μl and the UV detection wavelength was performed at 203 nm for all ginsenosides and PGSE Cell culture The human promonocytic U937 cells [19] were obtained from American...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... elements required for function in either T cells or mast cells Shown below are the positions that nucleosomes and GATA-2 occupy in unstimulated T cells and mast cells [85] a 30-nm chromatin fibre I have ... co-expressed active genes [182], plus their enhancers, and may in fact be no different to the active sites termed transcription factories [183,184] The co-localization of many active promoters and enhancers ... oocytes Cell 40, 923–932 112 Bloom KS & Anderson JN (1978) Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion Cell...
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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx
... the (A) MAPK signaling pathway, (B) Notch signaling pathway, (C) EcR signaling pathway, (D) Hippo signaling pathway, (E) TGF-b signaling pathway, (F) Hh signaling pathway, and (G) Wnt signaling ... and Notch signaling pathways in tumor development and progression Cancer Lett 292, 141–148 10 Hagen JW & Lai EC (2008) microRNA control of cell- cell signaling during development and disease Cell ... stimulation of miRNAs and cell signaling HER2 ⁄ neu signaling and that miR-21 suppresses the metastasis suppressor protein PDCD4 (programmed cell death 4) in breast cancer cells The expression...
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Báo cáo khoa học: Homoadenosylcobalamins as probes for exploring the active sites of coenzyme B12-dependent diol dehydratase and ethanolamine ammonia-lyase docx
... catalytic turnovers on average, and that this probability of inactivation is 3800 times higher than that with AdoCbl On the other hand, AdoEtCbl and AdePeCbl were totally inactive as coenzymes even ... apoenzyme (Fig 1A) [17] AdoMeCbl [24] and AdoEtCbl [29] were reported to be totally inactive as coenzyme for ribonucleotide reductase, and the latter inactive for diol dehydratase [27] The X-ray ... 36–44% and 38% as active as AdoCbl in diol dehydratase [27,43,44] and glycerol dehydratase [44], respectively, but it serves as a strong competitive inhibitor for ethanolamine ammonia-lyase [44] and...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... l-glutamine and 5% fetal bovine serum) and maintained at 37 C and 5% CO2 For translocation experiments, cells were removed with 0.05% trypsin EDTA, and plated with cell solution of ã 106 cellsặmL)1 ... prepared for cell assay and was added following the same procedure described for the time course cell assay The cells were washed with NaCl Pi and xed after 400 min, and the standard immunocytochemical ... regulators of immune and inammatory responses and also genes involved in apoptosis and cell proliferation [36] In this paper, we report the results of cell- based, in vitro and in silico experiments...
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Báo cáo khoa học: Classification of ATP-dependent proteases Lon and comparison of the active sites of their proteolytic domains pdf
... sequences for fragments of LonA, LonB, and Vp4 proteases that include the catalytically active Ser and Lys residues Catalytically active Ser (position 0) and Lys (position +43) residues are marked ... the domains The locations and sequences of the Walker A and B motifs (AAA+ module) and of fragments of the proteolytic domains including catalytically active serine (S*) and lysine (K*) residues ... their active sites [25] is correct; (b) the conserved Lys residue is a member of the catalytic Ser–Lys dyad; and (c) two Lon subfamilies, named LonA and LonB, can be identified LonA, LonB, and Lon-like...
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unsicker - cell signaling and growth factors in development
... XXXV Cell Signaling and Growth Factors in Development Edited by K Unsicker and K Krieglstein © 2006 WILEY-VCH Verlag GmbH & Co Part I Cell Signaling and Growth Factors in Development Cell Signaling ... Nails 688 18.2.6 Eccrine and Apocrine Sweat Glands 688 18.3 Cell Adhesion and the Role of Adhesion Molecules in Development 689 18.3.1 Cell- Cell Adhesion 689 18.3.2 Cell- Extracellular Matrix Adhesion ... 254 Cell Cycle Regulation and Neuronal Fate Determination 254 Cell Cycle of Neuroepithelial Cells 255 Interkinetic Nuclear Migration and Cell Division 255 Length of Cell Cycle of Neural Stem Cells...
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cancer cell signaling methods and protocols
... instead of feeder cells for a short period, particularly when we needed pure ES cells without feeder cells for biochemical analysis etc (see Note 1) 2.1.1 Cells R1 cells and STO cells were kindly ... Stock Thaw cells at 37ºC and wash once with medium Add mL of medium and pipeting Transfer cells into T-25 flask with feeder cells and culture at 37ºC, 5% CO2 Change medium at d or d and passage ... trypsin/EDTA and incubate for 10 at 37ºC Pipet the cells and transfer to a 24-well plate with feeder cells and mL of medium After the cells have grown to 50% confluence, wash once with PBS(-) and incubate...
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Báo cáo sinh học: "Combination immunotherapy and active-specific tumor cell vaccination augments anti-cancer immunity in a mouse model of gastric cancer" pdf
... IFN-g release and cell- mediated cytotoxicity after vaccination with mGC8 cells and GM-CSF T cells generated from TVDLN at day nine after vaccination were polyclonally activated and expanded as described ... gastric tumor cell lines mGC8 and 424GC Mice were vaccinated s.c with 107 irradiated tumor cells After weeks, vaccinated and control mice were s.c injected with × 106 viable tumor cells and tumor ... occurred in mGC8 cells and MCA310 cells to a similar level (Figure 3C) In contrast, LN cells from mGC8 GMCSF/IFA-treated mice induced specific lysis of mGC8 cells at an E:T ratio of 500:1 and 250:1...
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Cancer Cell Signaling Methods and Protocols potx
... Pathogens: Methods and Protocols, edited by Konrad Sachse and Joachim Frey, 2003 215 Cytokines and Colony Stimulating Factors: Methods and Protocols, edited by Dieter Körholz and Wieland Kiess, 2003 ... transfected 3.3.2 Cell Transfection Use µg of CMV-vector or CMV-RB and 0.5 µg of CMV-H2B-GFP (see Note 5) Use 0.25 mL CaCl2 and 0.25 mL 2X BBS 10 Knudsen and Angus 3.3.3 Cell Harvesting and Fixation ... Neural Stem Cells: Methods and Protocols, edited by Tanja Zigova, Juan R Sanchez-Ramos, and Paul R Sanberg, 2002 197 Mitochondrial DNA: Methods and Protocols, edited by William C Copeland, 2002...
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SỰ TRUYỀN TÍN HIỆU VÀ GIAO LƯU THÔNG TIN TẾ BÀO (CELL SIGNALING AND COMMUNICATION) pps
... đa tế bào với ligand đặc biệt nói chung xảy nồng độ ligand hầu hết receptor chưa bị choán chỗ Các ligand Ligand (phối tử) phân tử tín hiệu gắn vào thụ thể Các receptor gắn ligand với tính đặc ... tác không đồng hoá trị ligand amino acid đặc trưng protein thụ thể Sự gắn ligand làm cho thụ thể biến dạng Ligand không tiếp tục tham gia vào đường Các receptor gắn ligand theo quy luật tác động ... nghịch Nồng độ ligand làm phân nửa receptor bị choán chỗ gắn kết với ligand, Kd, xác định thực nghiệm biểu thị lực ligand với receptor Các tác nhân ức chế gắn vào chỗ gắn ligand phân tử receptor...
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Báo cáo y học: " Eph Receptors and Ephrin Signaling" pps
... ephrinA and ephrinB ligands, and ephrinA5 which can 264 also bind to EphB2 [2, 3] Figure Domain structure of Eph receptors and ephrinA and ephrinB ligands Eph receptors have an extracellular ... mice [51] EphA receptors and their ligands are expressed by T cells and are thought to regulate signaling through the T cell receptor [52, 53] While expression of Eph receptors and ephrins has ... important in mediating Eph receptors and integrin signaling [7] Reverse Signaling The interaction between ephrin ligands and Eph receptors results not only in forward signaling through the Eph...
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báo cáo khoa học: "Different patterns of NF-B and Notch1 signaling contribute to tumor-induced lymphangiogenesis of esophageal squamous cell carcinoma" ppt
... suggests that Notch signaling guides endothelial cells and associated mural cells through the cell- fate decisions needed to form and maintain the vascular system [16] Although Notch signaling anomalies ... tumor cells in ESCC Lymphangiogenetic characteristics and their associations with NF-B and Notch1 signaling were also measured to determine Page of the contribution of NF-B and Notch signaling ... ZC and HL conceived of the study; CS, YS, YL, YL and BZ performed experiments; ZC and LC analyzed data and prepared the figures; CS, ZC and HL drafted the manuscript All authors have read and...
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Báo cáo y học: "Mechanical signals control SOX-9, VEGF, and c-Myc expression and cell proliferation during inflammation via integrin-linked kinase, B-Raf, and ERK1/2-dependent signaling in articular chondrocytes" pot
... (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes J Cell Biol 2001, 155:505-510 Chowdhury TT, Arghandawi S, Brand ... many cell surface receptors, such as growth factor receptors, receptor tyrosine kinases, integrins, and IL-6 receptors [34-36], further suggesting that dynamic mechanical signals activate signaling ... PCR and the 2(-delta delta C(T)) method Methods 2001, 25:402-408 Li F, Zhang Y, Wu C: Integrin-linked kinase is localized to cell- matrix focal adhesions but not cell- cell adhesion sites and the...
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Báo cáo y học: "The association of endothelial cell signaling, severity of illness, and organ dysfunction in sepsis" ppt
... transmigrate through and/ or between endothelial cells into the underlying tissue (not shown) In sepsis, E-selectin, ICAM-1, and VCAM-1 are cleaved from the cell surface and circulate as a soluble ... factor (VEGF) signaling Under normal conditions (quiescence), VEGF signaling plays a critical role in homeostasis VEGF binds to two receptors on endothelial cells, VEGF receptor (VEGFR) and VEGFR1 ... VEGF signaling in endothelial cells results in an activation phenotype, including increased permeability, induction of cell adhesion molecules [42-44], the release of cytokines and chemokines, and...
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Báo cáo y học: " Cell signaling and cancer" pot
... Bazigou and Rallis that regulate cell adhesion, cell tension and cell protrusion, and several signaling networks were identified as possible regulators of particular morphological changes and behaviors ... efficient and reproducible, and is compatible with standard analyses and commonly used scanners, microscopes and imagers Using this platform, breast cancer cell lines have been screened by RNAi and ... cells He described a novel RNAi screening platform using miniaturized arrays of 10,000 spots of 150-250 cells each, which can be assessed for parameters such as cell numbers, cell death, or cell...
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DEATH-ASSOCIATED PROTEIN KINASE REGULATES VASCULAR SMOOTH MUSCLE CELL SIGNALING AND MIGRATION
... of cell adhesion and migration, however these studies have been performed in cancer cells and fibroblasts (70, 71, 121, 123) While DAPK is expressed in smooth muscle cells, and cell adhesion and ... insulin, and 0.5 ng/ml human EGF EGF, insulin, and bFGF were obtained from Invitrogen HeLa cells were purchased from ATCC and were cultured in DMEM (Mediatech) containing 10% FCS and glutamine and ... cell migration that may be regulated by DAPK signaling, and ultimately the pathways DAPK impacts in primary smooth muscle cells In this screen, the mRNA levels of extracellular matrix (ECM) and...
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Computational modeling of cell signaling dynamics hypothesis management and parameter estimation methods applied to the akt pathway
... structure and functions of these networks are among the most active topics currently being studied around the world Cell signaling describes critical processes regulating basic cellular mechanisms and ... about signaling pathways, including Science Signaling s Database of Cell Signaling (AAAS and Stanford, 2012), KEGG PATHWAY database (Kanehisa, et al., 2012), SignaLink (Farkas, et al., 2011), and ... SDS-PAGE and Western blot analysis were used to detect the phosphorylation level of Akt, total level of Akt, and total level of PDK1 Cells were washed once with cold 1x PBS and collected in PBS The cell...
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Protein s nitrosylation and its relevance to redox control of cell signaling
... SOURCES AND FORMATION OF REACTIVE OXYGEN AND NITROGEN SPECIES 1.2.1 1.2.2 Hydrogen Peroxide and Hydroxyl Radical 1.2.3 1.3 Superoxide Nitric Oxide and its derivatives EFFECTS OF REACTIVE OXYGEN AND ... NITROGEN SPECIES ON CELLULAR STRACTURE AND SIGNALING 1.3.1 1.3.2 1.4 Cellular Toxicity Physiological Function: Redox Signaling 10 MECHANISMS OF REDOX-BASED REGULATION OF CELL SIGNALING: FUNCTIONAL ... control of cell signaling Understanding the mechanism of how free radicals regulate signaling is critical to distinguish between normal physiology and cellular toxicity both caused by reactive species...
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